Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	302,016	G→T	V186V (GTC→GTA)	paoA ←	aldehyde dehydrogenase, Fe‑S subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	302,016	0	G	T	100.0%	173.3 / NA	50	V186V (GTC→GTA)	paoA	aldehyde dehydrogenase, Fe‑S subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (16/34);  total (16/34)

CCTTCATGATTTTATCTCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGGCCGCCTGCATGGGG  >  NC_000913/301881‑302142                                                                                                                                        |                                                                                                                               ccTTCATGATTTTATCTCCCCCGCAGCTTCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCTACcg                                                                                                                            <  2:717244/140‑1 (MQ=255)          ttttATCTCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCTACCgtgacgtgac                                                                                                                   <  1:224743/140‑1 (MQ=255)             tATCTCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTgg                                                                                                                <  2:180164/140‑1 (MQ=255)                  cccccGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATg                                                                                                           <  1:70498/140‑1 (MQ=255)                   ccccGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGc                                                                                                          <  1:718038/140‑1 (MQ=255)                    cccGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCACTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGcc                                                                                                         >  2:512553/1‑140 (MQ=255)                     ccGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATg                                                                                                           <  2:731108/137‑1 (MQ=255)                     ccGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATg                                                                                                           >  1:731108/1‑137 (MQ=255)                      cGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGCCGt                                                                                                       >  2:557043/1‑140 (MQ=255)                            aTCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGCCGTCCTGaa                                                                                                 <  2:586846/140‑1 (MQ=255)                            aTCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGCCGTCCTGaa                                                                                                 <  2:300073/140‑1 (MQ=255)                            aTCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGCCGTCCTGaa                                                                                                 <  1:557043/140‑1 (MQ=255)                            aTCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGCCGTCCTGaa                                                                                                 <  2:576625/140‑1 (MQ=255)                              cTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGCCGTCCTGAAtc                                                                                               <  2:521408/140‑1 (MQ=255)                                 cAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGCCGTCCTGAATctct                                                                                            >  1:183228/1‑140 (MQ=255)                                       cggcAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGc                                                                                      >  1:497175/1‑140 (MQ=255)                                       cggcAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGc                                                                                      <  1:577/140‑1 (MQ=255)                                       cggcAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGc                                                                                      <  1:226875/140‑1 (MQ=255)                                       cggcAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGc                                                                                      >  1:769497/1‑140 (MQ=255)                                        ggcAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCa                                                                                     >  2:541091/1‑140 (MQ=255)                                              gATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTa                                                                               <  2:332607/140‑1 (MQ=255)                                                                  aGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCg                                                           >  2:592083/1‑140 (MQ=255)                                                                  aGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCg                                                           >  1:400990/1‑140 (MQ=255)                                                                    cGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGa                                                         <  2:585611/140‑1 (MQ=255)                                                                    cGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGa                                                         >  1:281499/1‑140 (MQ=255)                                                                    cGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGa                                                         >  1:524503/1‑140 (MQ=255)                                                                     gACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAg                                                        <  1:847645/140‑1 (MQ=255)                                                                     gACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAg                                                        <  1:541091/140‑1 (MQ=255)                                                                     gACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAg                                                        <  1:536833/140‑1 (MQ=255)                                                                           tGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAg                                                  <  1:489157/140‑1 (MQ=255)                                                                               gCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGc                                              >  2:452648/1‑140 (MQ=255)                                                                                 cGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCg                                            >  1:469222/1‑140 (MQ=255)                                                                                    tCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGGGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGGCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCAc                                         >  2:393043/1‑140 (MQ=255)                                                                                     cATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACt                                        >  2:253760/1‑140 (MQ=255)                                                                                        aCGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGa                                     <  1:592083/140‑1 (MQ=255)                                                                                         cGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGaa                                    <  2:317055/140‑1 (MQ=255)                                                                                          gTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAg                                   <  1:393043/140‑1 (MQ=255)                                                                                             cACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCca                                <  1:452648/140‑1 (MQ=255)                                                                                               cGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCcatc                              <  2:497175/140‑1 (MQ=255)                                                                                                  aTCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCTAGCGTGACGTGTCTGGGAATGCCGTACTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTTGAAGCcatcaca                           >  2:41820/1‑138 (MQ=255)                                                                                                   tctcATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGc                          <  2:183228/140‑1 (MQ=255)                                                                                                         cGGCAGTTGTTTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATa                    <  2:281499/140‑1 (MQ=255)                                                                                                            cAGTTGTTTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAg                 >  2:321265/1‑140 (MQ=255)                                                                                                              gttgttTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGgc               <  2:670881/140‑1 (MQ=255)                                                                                                               ttgttTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAGTGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGgcc              >  2:403920/1‑140 (MQ=255)                                                                                                                tgttTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGgccg             <  2:62805/140‑1 (MQ=255)                                                                                                                tgttTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGgccg             <  1:321265/140‑1 (MQ=255)                                                                                                                tgttTCTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGgccg             <  2:734052/140‑1 (MQ=255)                                                                                                                     cTGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGGCCGCCTGc        <  2:400990/140‑1 (MQ=255)                                                                                                                      tGGAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGGCCGCCTGCa       <  2:838752/140‑1 (MQ=255)                                                                                                                       ggAGCGGAAACCAAATCTACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGGCCGCCTGCAt      <  1:253760/140‑1 (MQ=255)                                                                                                                           cGGAAACCAAATCTACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGGCCGCCTGCATgggg  <  2:469222/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                               CCTTCATGATTTTATCTCCCCCGCAGCATCTTCAATGGCGGCAAGGATGTTAGCGTATGCACCACAGCGACAGATGTTGCCGCTCATACGTTCACGGATCTCATCGGCAGTTGTTTCTGGAGCGGAAACCAAATCGACCGTGACGTGACTGGGAATGCCGTCCTGAATCTCTTTTAGCACCGCTACTGATGAGCAAATTTGCCCGGAGGTGCAGTAGCCGCACTGGAAGCCATCATGCTTGATAAAGGCCGCCTGCATGGGG  >  NC_000913/301881‑302142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑