Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,230,822	G→T	S19I (AGC→ATC)	umuD →	DNA polymerase V protein UmuD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,230,822	0	G	T	100.0%	262.6 / NA	73	S19I (AGC→ATC)	umuD	DNA polymerase V protein UmuD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (43/30);  total (43/30)

AGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGG  >  NC_000913/1230691‑1230957                                                                                                                                    |                                                                                                                                        aGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTg                                                                                                                                 >  2:244280/1‑140 (MQ=255)   ccTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGtt                                                                                                                               >  1:334321/1‑140 (MQ=255)   ccTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGtt                                                                                                                               <  1:323513/140‑1 (MQ=255)      gAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAg                                                                                                                            >  1:184951/1‑140 (MQ=255)       aaTCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAgt                                                                                                                           >  2:466669/1‑140 (MQ=255)                     tgctgGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCa                                                                                                             >  2:181864/1‑140 (MQ=255)                       ctgGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCAcc                                                                                                           >  1:154915/1‑140 (MQ=255)                       ctgGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCAcc                                                                                                           >  2:171067/1‑140 (MQ=255)                       ctgGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCAcc                                                                                                           <  2:184951/140‑1 (MQ=255)                       ctgGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCAcc                                                                                                           >  2:470344/1‑140 (MQ=255)                                aCAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGa                                                                                                  <  2:334321/140‑1 (MQ=255)                                aCAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGa                                                                                                  <  2:625816/140‑1 (MQ=255)                                 cAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGAt                                                                                                 >  2:49427/1‑140 (MQ=255)                                 cAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGAt                                                                                                 >  2:176514/1‑140 (MQ=255)                                         tGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGa                                                                                         >  1:766061/1‑140 (MQ=255)                                          gTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGaa                                                                                        <  2:604424/140‑1 (MQ=255)                                          gTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGaa                                                                                        <  1:49427/140‑1 (MQ=255)                                            atataAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACa                                                                                      <  2:149568/140‑1 (MQ=255)                                              ataAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAgc                                                                                    >  1:104165/1‑140 (MQ=255)                                              ataAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAgc                                                                                    >  1:96365/1‑140 (MQ=255)                                              ataAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAgc                                                                                    >  1:616816/1‑140 (MQ=255)                                                 aaaaCAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCa                                                                                 <  2:96365/140‑1 (MQ=255)                                                  aaaCAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCAt                                                                                >  1:161273/1‑140 (MQ=255)                                                  aaaCAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCAt                                                                                >  1:725669/1‑140 (MQ=255)                                                  aaaCAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCAt                                                                                >  1:232295/1‑140 (MQ=255)                                                  aaaCAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCAt                                                                                >  1:279239/1‑140 (MQ=255)                                                  aaaCAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCAt                                                                                >  1:787852/1‑140 (MQ=255)                                                  aaaCAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCAt                                                                                >  1:93070/1‑140 (MQ=255)                                                     cAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGa                                                                             <  1:181864/140‑1 (MQ=255)                                                     cAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGa                                                                             >  2:589626/1‑140 (MQ=255)                                                      aGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGAt                                                                            <  1:708774/140‑1 (MQ=255)                                                       gTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATc                                                                           >  2:192464/1‑140 (MQ=255)                                                        tataACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCt                                                                          >  2:270007/1‑140 (MQ=255)                                                            aCTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAAt                                                                      <  2:725669/140‑1 (MQ=255)                                                             cTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATc                                                                     >  2:212467/1‑140 (MQ=255)                                                              ttCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCa                                                                    <  2:232295/140‑1 (MQ=255)                                                                cAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAAc                                                                  >  2:465266/1‑140 (MQ=255)                                                                  ggCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTg                                                                >  1:33934/1‑140 (MQ=255)                                                                    cAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGtt                                                              <  1:358293/140‑1 (MQ=255)                                                                       attattATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGa                                                            >  2:334691/1‑139 (MQ=255)                                                                       attattATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGa                                                            <  1:334691/139‑1 (MQ=255)                                                                                 tgttTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATccc                                                 >  2:793811/1‑140 (MQ=255)                                                                                 tgttTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATccc                                                 >  1:730718/1‑140 (MQ=255)                                                                                 tgttTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATccc                                                 >  2:204568/1‑140 (MQ=255)                                                                                   tttATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAg                                               >  1:477939/1‑140 (MQ=255)                                                                                   tttATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAg                                               >  1:68754/1‑140 (MQ=255)                                                                                   tttATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAg                                               >  1:770801/1‑140 (MQ=255)                                                                                   tttATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAg                                               >  1:777415/1‑140 (MQ=255)                                                                                   tttATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAg                                               >  1:847362/1‑140 (MQ=255)                                                                                   tttATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAg                                               >  1:414154/1‑140 (MQ=255)                                                                                     tATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAgcg                                             >  2:343415/1‑140 (MQ=255)                                                                                     tATCAAGCCTGCGGATCTCCGCGAAATTGTGACCTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAgcg                                             >  2:664437/1‑140 (MQ=255)                                                                                            ccTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGactta                                      <  2:93070/140‑1 (MQ=255)                                                                                                cGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTc                                  >  2:406314/1‑140 (MQ=255)                                                                                                cGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTc                                  >  1:575784/1‑140 (MQ=255)                                                                                                   aTCTCCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTc                               <  2:730718/140‑1 (MQ=255)                                                                                                      tcCGCGAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCaaa                            >  2:744489/1‑140 (MQ=255)                                                                                                         gcgAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCa                         <  2:154915/140‑1 (MQ=255)                                                                                                           gAAATTGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAg                       <  2:104165/140‑1 (MQ=255)                                                                                                               ttGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAgtggt                   <  1:192464/140‑1 (MQ=255)                                                                                                               ttGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAgtggt                   <  1:212467/140‑1 (MQ=255)                                                                                                               ttGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAgtggt                   <  1:577882/140‑1 (MQ=255)                                                                                                               ttGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAgtggt                   <  1:466669/140‑1 (MQ=255)                                                                                                               ttGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAgtggt                   >  2:373175/1‑140 (MQ=255)                                                                                                               ttGTGACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAgtggt                   >  2:2443/1‑140 (MQ=255)                                                                                                                 gtgACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGa                 <  2:720959/140‑1 (MQ=255)                                                                                                                 gtgACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGa                 <  1:793811/140‑1 (MQ=255)                                                                                                                   gACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGAtt               <  1:406314/140‑1 (MQ=255)                                                                                                                   gACTTTTCCGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGAtt               <  2:33934/140‑1 (MQ=255)                                                                                                                          ccGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTAtgat        <  2:575784/140‑1 (MQ=255)                                                                                                                          ccGCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTAtgat        <  1:171067/140‑1 (MQ=255)                                                                                                                            gCTATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTAtgattg      <  2:616816/140‑1 (MQ=255)                                                                                                                              tATTTATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTAtgattgat    <  2:847362/140‑1 (MQ=255)                                                                                                                                tttATCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGAtgg  <  2:786492/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                        AGCCTGAATCAGTATTGATCTGCTGGCAAGAACAGACTACTGTATATAAAAACAGTATAACTTCAGGCAGATTATTATGTTGTTTATCAAGCCTGCGGATCTCCGCGAAATTGTGACTTTTCCGCTATTTAGCGATCTTGTTCAGTGTGGCTTTCCTTCACCGGCAGCAGATTACGTTGAACAGCGCATCGATCTGAATCAACTGTTGATCCAGCATCCCAGCGCGACTTACTTCGTCAAAGCAAGTGGTGATTCTATGATTGATGG  >  NC_000913/1230691‑1230957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑