Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,658,572	G→T	M168I (ATG→ATT)	ynfE →	putative selenate reductase YnfE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,658,572	0	G	T	100.0%	180.6 / NA	52	M168I (ATG→ATT)	ynfE	putative selenate reductase YnfE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (29/23);  total (29/23)

GAAGGCAAATTCGAACGGATTAGCTGGGATGAAGCCCTGGATACTATCGCCAGTAGCCTGAAGAAAACCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATG  >  NC_000913/1658438‑1658702                                                                                                                                       |                                                                                                                                   gAAGGCAAATTCGAACGGATTAGCTGGGATGAAGCCCTGGATACTATCGCCAGTAGCCTGAAGAAAACCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATTACCCg                                                                                                                               <  2:106718/140‑1 (MQ=255)    ggCAAATTCGAACGGATTAGCTGGGATGAAGCCCTGGATACTATCGCCAGTAGCCTGAAGAAAACCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGctc                                                                                                                            >  1:749719/1‑140 (MQ=255)               aCGGATTAGCTGGGATGAAGCCCTGGATACTATCGCCAGTAGCCTGAAGAAAACCGTCGAACAATATGGCAATGAGGCTGTATACATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAg                                                                                                                 >  2:576792/1‑140 (MQ=255)                             aTGAAGCCCTGGATACTATCGCCAGTAGCCTGAAGAAAACCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCaaa                                                                                                   >  1:690492/1‑140 (MQ=255)                             aTGAAGCCCTGGATACTATCGCCAGTAGCCTGAAGAAAACCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCaaa                                                                                                   >  1:632472/1‑140 (MQ=255)                             aTGAAGCCCTGGATACTATCGCCAGTAGCCTGAAGAAAACCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCaaa                                                                                                   >  1:45077/1‑140 (MQ=255)                                      tGGATACTATCGCCAGTAGCCTGAAGAAAACCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCtgatg                                                                                          >  2:491281/1‑140 (MQ=255)                                        gATACTATCGCCAGTAGCCTGAAGAAAACCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGaa                                                                                        <  1:236632/140‑1 (MQ=255)                                           aCTATCGCCAGTAGCCTGAAGAAAACCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTg                                                                                     <  2:451804/140‑1 (MQ=255)                                             tATCGCCAGTAGCCTGAAGAAAACCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAAc                                                                                       >  2:371281/1‑136 (MQ=255)                                             tATCGCCAGTAGCCTGAAGAAAACCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAAc                                                                                       <  1:371281/136‑1 (MQ=255)                                             tATCGCCAGTAGCCTGAAGAAAACCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCt                                                                                   >  2:286127/1‑140 (MQ=255)                                              aTCGCCAGTAGCCTGAAGAAAACCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTa                                                                                  >  2:251097/1‑140 (MQ=255)                                                  ccAGTAGCCTGAAGAAAACCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTAcggc                                                                              >  2:678079/1‑140 (MQ=255)                                                      tAGCCTGAAGAAAACCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTAcggcgggt                                                                          >  1:353637/1‑138 (MQ=255)                                                           tGAAGAAAACCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTc                                                                     <  2:454972/140‑1 (MQ=255)                                                           tGAAGAAAACCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGGTCACTc                                                                     >  1:718439/1‑140 (MQ=255)                                                            gaagaaAACCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCa                                                                    <  1:812548/140‑1 (MQ=255)                                                             aagaaAACCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTTAcccca                                                                   >  2:737883/1‑136 (MQ=255)                                                              agaaAACCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAAc                                                                  <  1:286127/140‑1 (MQ=255)                                                                aaaaCCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTAcggcgg                                                                            >  2:802093/1‑128 (MQ=255)                                                                aaaaCCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTAcggcgg                                                                            <  1:802093/128‑1 (MQ=255)                                                                  agccGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGt                                                              <  1:305990/138‑1 (MQ=255)                                                                  aaCCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGt                                                              <  2:591091/140‑1 (MQ=255)                                                                       tCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGc                                                         <  1:737883/140‑1 (MQ=255)                                                                       tCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGc                                                         <  2:799592/140‑1 (MQ=255)                                                                       tCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGc                                                         <  1:491281/140‑1 (MQ=255)                                                                       tCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGc                                                         >  1:351910/1‑140 (MQ=255)                                                                          aaCAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGGTCACTCCACCCGGATgggccc                                                      >  2:481557/1‑136 (MQ=255)                                                                                    cAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTg                                            <  2:351910/140‑1 (MQ=255)                                                                                         aGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGGATGGCTCCCTCAGCACTgcgcca                                       >  1:571857/1‑138 (MQ=255)                                                                                         aGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGGTCACTCAACCACTATGGCTCCCACAACACCGCGCaa                                       >  2:481494/1‑140 (MQ=255)                                                                                         aGGCTGTATACATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCaa                                       <  1:576792/140‑1 (MQ=255)                                                                                            cTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCCACCACTATGGCGCCCACAAGACTGCGCCAAtt                                    >  2:147708/1‑140 (MQ=255)                                                                                                atatatTCAGTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCt                                <  1:283636/140‑1 (MQ=255)                                                                                                atatatTCAGTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCt                                <  1:238130/140‑1 (MQ=255)                                                                                                     ttCAGTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTTCCTCAAACCGGATGGCTCCCACAGCACCGCGCCAATTTTCTTTGcc                           >  1:435286/1‑140 (MQ=255)                                                                                                     ttCAGTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGcc                           <  1:102876/140‑1 (MQ=255)                                                                                                      tCAGTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCa                          <  1:508880/140‑1 (MQ=255)                                                                                                       cAGTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCCAATTTTCTGTGccct                         >  1:310758/1‑138 (MQ=255)                                                                                                         gTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTTACTCAACCAAGATGGGTCCTCCAGGACTGCGCAAAATTTCTTTGCCCTGc                       >  2:238130/1‑140 (MQ=255)                                                                                                         gTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCCCTCAACCAGTATGGCGCCCCCAGCACCGCGCAAAATTTCTTTGcccatc                       >  2:450961/1‑136 (MQ=255)                                                                                                         gTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGcc                           <  2:52748/136‑1 (MQ=255)                                                                                                         gTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGcc                           >  1:52748/1‑136 (MQ=255)                                                                                                         gTACTCTTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGGTCACCCCACCCGTATGGCTCCTACAACACCGCGCCAATTTTCTTTGCCCTGc                       >  2:518089/1‑140 (MQ=255)                                                                                                            ctctTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATGCCCt                    <  1:147708/140‑1 (MQ=255)                                                                                                             tctTCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTTCTGtgccctgcccca                   >  1:263443/1‑138 (MQ=255)                                                                                                               ttCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCACAAATTTCCTGTGCCATGCCCTaca                 >  1:100052/1‑140 (MQ=255)                                                                                                               ttCGGGGATCGTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGGTTACTCCACCAATATGGGTCCCTCAACACCGCGCCAAATTTCTTTGGCCTGCCCCacc                 >  2:641816/1‑139 (MQ=255)                                                                                                                        cGTTGGCGGCAATATTACCCGCTCTGCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCa        <  2:632472/140‑1 (MQ=255)                                                                                                                         gTTGGCGGCAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGGTCACTCAAACAGTATTGGTCCTACAACACCGCGCCAAATTTCTTTGCCCTTCCCCACACCCACCGCAg       >  2:108831/1‑140 (MQ=255)                                                                                                                              cggcAATATTACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAatg  <  2:690492/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                   GAAGGCAAATTCGAACGGATTAGCTGGGATGAAGCCCTGGATACTATCGCCAGTAGCCTGAAGAAAACCGTCGAACAATATGGCAATGAGGCTGTATATATTCAGTACTCTTCGGGGATCGTTGGCGGCAATATGACCCGCTCTTCGCCATCAGCCTCGGCGGTCAAACGCCTGATGAACTGCTACGGCGGTTCACTCAACCAGTATGGCTCCTACAGCACTGCGCAAATTTCCTGTGCCATGCCCTACACCTACGGCAGTAATG  >  NC_000913/1658438‑1658702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑