Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,820,775	G→T	A147E (GCG→GAG)	chbC ←	N,N'‑diacetylchitobiose‑specific PTS enzyme IIC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,820,775	0	G	T	100.0%	130.6 / NA	39	A147E (GCG→GAG)	chbC	N,N'‑diacetylchitobiose‑specific PTS enzyme IIC component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (17/22);  total (17/22)

GGAATTAATGCCGAGAAGGAACGCGATACTGAAGCAGGTACGCTGTCGGGCAGTTTAATGACCCAATTGCGGCGGACAATAAAGGTAAACATTTCTGCCACCACCAGGCCAATAATAATCCCGGAGATGATATTCGCCCCACCTAACCAGTTTGCACCAACCGCATAGGCCTCACCGACACTATATGGGGTGACGGTCATAAATGCTGCAACGGATAACAACCCAGCCGCCAGCGCATCGACTTTACGCTCTTCTGCCAGCGCCATGCCA  >  NC_000913/1820640‑1820909                                                                                                                                        |                                                                                                                                       ggAATTAATGCCGAGAAGGAACGCGATACTGAAGCAGGTACGCTGTCGGGCAGTTTAATGACCCAATTGCGGCGGACAATAAAGGTAAACATTTCTGCCACCACCAGGCCAATAATAATCCCGGAGATGATATTCTcccc                                                                                                                                    >  2:4955/1‑140 (MQ=255) ggAATTAATGCCGAGAAGGAACGCGATACTGAAGCAGGTACGCTGTCGGGCAGTTTAATGACCCAATTGCGGCGGACAATAAAGGTAAACATTTCTGCCACCACCAGGCCAATAATAATCCCGGAGATGATATTCTcccc                                                                                                                                    <  1:292428/140‑1 (MQ=255) ggAATTAATGCCGAGAAGGAACGCGATACTGAAGCAGGTACGCTGTCGGGCAGTTTAATGACCCAATTGCGGCGGACAATAAAGGTAAACATTTCTGCCACCACCAGGCCAATAATAATCCCGGAGATGATATTCTcccc                                                                                                                                    >  1:539430/1‑140 (MQ=255)       aaTGCCGAGAAGGAACGCGATACTGAAGCAGGTACGCTGTCGGGCAGTTTAATGACCCAATTGCGGCGGACAATAAAGGTAAACATTTCTGCCACCACCAGGCCAATAATAATCCCGGAGATGATATTCTCCCCACCTaa                                                                                                                              <  1:705141/140‑1 (MQ=255)                          aTACTGAAGCAGGTACGCTGTCGGGCAGTTTAATGACCCAATTGCGGCGGACAATAAAGGTAAACATTTCTGCCACCACCAGGCCAATAATAATCCCGGAGATGATATTCTCCCCACCTAACCAGTTTGc                                                                                                                     <  2:414562/130‑1 (MQ=255)                          aTACTGAAGCAGGTACGCTGTCGGGCAGTTTAATGACCCAATTGCGGCGGACAATAAAGGTAAACATTTCTGCCACCACCAGGCCAATAATAATCCCGGAGATGATATTCTCCCCACCTAACCAGTTTGc                                                                                                                     >  1:414562/1‑130 (MQ=255)                                 aGCAGGTACGCTGTCGGGCAGTTTAATGACCCAATTGCGGCGGACAATAAAGGTAAACATTTCTGCCACCACCAGGCCAATAATAATCCCGGAGATGATATTCTCCCCACCTAACCAGTTTGCACCAACCGCATAGGCCt                                                                                                    >  2:146277/1‑140 (MQ=255)                                  gCAGGTACGCTGTCGGGCAGTTTAATGACCCAATTGCGGCGGACAATAAAGGTAAACATTTCTGCCACCACCAGGCCAATAATAATCCCGGAGATGATATTCTCCCCACCTAACCAGTTTGCACCAACCGCATAGGCCTc                                                                                                   >  1:823877/1‑140 (MQ=255)                                    aGGTACGCTGTCGGGCAGTTTAATGACCCAATTGCGGCGGACAATAAAGGTAAACATTTCTGCCACCACCAGGCCAATAATAATCCCGGAGATGATATTCTCCCCACCTAACCAGTTTGCACCAACCGCATAGGCCTCAc                                                                                                 <  1:42173/140‑1 (MQ=255)                                     ggTACGCTGTCGGGCAGTTTAATGACCCAATTGCGGCGGACAATAAAGGTAAACATTTCTGCCACCACCAGGCCAATAATAATCCCGGAGATGATATTCTCCCCACCTAACCAGTTTGCACCAACCGCATAGGCCTCAcc                                                                                                >  1:325777/1‑140 (MQ=255)                                     ggTACGCTGTCGGGCAGTTTAATGACCCAATTGCGGCGGACAATAAAGGTAAACATTTCTGCCACCACCAGGCCAATAATAATCCCGGAGATGATATTCTCCCCACCTAACCAGTTTGCACCAACCGCATAGGCCTCAcc                                                                                                <  1:4955/140‑1 (MQ=255)                                     ggTACGCTGTCGGGCAGTTTAATGACCCAATTGCGGCGGACAATAAAGGTAAACATTTCTGCCACCACCAGGCCAATAATAATCCCGGAGATGATATTCTCCCCACCTAACCAGTTTGCACCAACCGCATAGGCCTCAcc                                                                                                <  1:639444/140‑1 (MQ=255)                                       tACGCTGTCGGGCAGTTTAATGACCCAATTGCGGCGGACAATAAAGGTAAACATTTCTGCCACCACCAGGCCAATAATAATCCCGGAGATGATATTCTCCCCACCTAACCAGTTTGCACCAACCGCATAGGCCTCACCGa                                                                                              <  2:426034/140‑1 (MQ=255)                                        aCGCTGTCGGGCAGTTTAATGACCCAATTGCGGCGGACAATAAAGGTAAACATTTCTGCCACCACCAGGCCAATAATAATCCCGGAGATGATATTCTCCCCACCTAACCAGTTTGCACCAACCGCATAGGCCTCACCGac                                                                                             <  2:823877/140‑1 (MQ=255)                                          gCTGTCGGGCAGTTTAATGACCCAATTGCGGCGGACAATAAAGGTAAACATTTCTGCCACCACCAGGCCAATAATAATCCCGGAGATGATATTCTCCCCACCTAACCAGTTTGCACCAACCGCATAGGCCTCACCGacac                                                                                           >  1:21360/1‑140 (MQ=255)                                          acgctggggCAGTTTAATGACCCAATTGCGGCGGACAATAAAGGTAAACATTTCTGCCACCACCAGGCCAATAATAATCCCGGAGATGATATTCTCCCCACCTAACCAGTTTGCACCAACCGCATAGGCCTCACCGacac                                                                                           <  1:586393/134‑1 (MQ=255)                                           cTGTCGGGCAGTTTAATGACCCAATTGCGGCGGACAATAAAGGTAAACATTTCTGCCACCACCAGGCCAATAATAATCCCGGAGATGATATTCTCCCCACCTAACCAGTTTGCACCAACCGCATAGGCCTCACCGACACt                                                                                          <  1:565229/140‑1 (MQ=255)                                            tGTCGGGCAGTTTAATGACCCAATTGCGGCGGACAATAAAGGTAAACATTTCTGCCACCACCAGGCCAATAATAATCCCGGAGATGATATTCTCCCCACCTAACCAGTTTGCACCAACCGCATAGGCCTCACCGACACta                                                                                         >  2:29736/1‑140 (MQ=255)                                               cGGGCAGTTTAATGACCCAATTGCGGTGGACAATAAAGGTAAACATTTCTGCCACCACCAGGCCAATAATAATCCCGGAGATGATATTCTCCCCACCTAACCAGTTTGCACCAACCGCATAGGCCTCACCGACACTatat                                                                                      <  1:207993/140‑1 (MQ=255)                                                   cAGTTTAATGACCCAATTGCGGCGGACAATAAAGGTAAACATTTCTGCCACCACCAGGCCAATAATAATCCCGGAGATGATATTCTCCCCACCTAACCAGTTTGCACCAACCGCATAGGCCTCACCGACACTATATgggg                                                                                  <  1:607237/140‑1 (MQ=255)                                                             aCCCAATTGCGGCGGACAATAAAGGTAAACATTTCTGCCACCACCAGGCCAATAATAATCCCGGAGATGATATTCTCCCCACCTAACCAGTTTGCACCAACCGCATAGGCCTCACCGACACTATATGGGGTGACGGTCAt                                                                        <  2:494983/140‑1 (MQ=255)                                                              cccAATTGCGGCGGACAATAAAGGTAAACATTTCTGCCACCACCAGGCCAATAATAATCCCGGAGATGATATTCTCCCCACCTAACCAGTTTGCACCAACCGCATAGGCCTCACCGACACTATATGGGGTGACGGTCATa                                                                       >  2:650741/1‑140 (MQ=255)                                                                cAATTGCGGCGGACAATAAAGGTAAACATTTCTGCCACCACCAGGCCAATAATAATCCCGGAGATGATATTCTCCCCACCTAACCAGTTTGCACCAACCGCATAGGCCTCACCGACACTATATGGGGTGACGGTCATaaa                                                                     >  2:780738/1‑140 (MQ=255)                                                                  aTTGCGGCGGACAATAAAGGTAAACATTTCTGCCACCACCAGGCCAATAATAATCCCGGAGATGATATTCTCCCCACCTAACCAGTTTGCACCAACCGCATAGGCCTCACCGACACTATATGGGGTGACGGTCATAAAtg                                                                   <  2:362247/140‑1 (MQ=255)                                                                       ggcggACAATAAAGGTAAACATTTCTGCCACCACCAGGCCAATAATAATCCCGGAGATGATATTCTCCCCACCTAACCAGTTTGCACCAACCGCATAGGCCTCACCGACACTATATGGGGTGACGGTCATAAATGCTGCa                                                              <  2:325777/140‑1 (MQ=255)                                                                         cggACAATAAAGGTAAACATTTCTGCCGCCACCAGGCCAATAATAATCCCGGAGATGATATTCTCCCCACCTAACCAGTTTGCACCAACCGCATAGGCCTCACCGACACTATATGGGGTGACGGTCATAAATGCTGCAAc                                                            <  1:650741/140‑1 (MQ=255)                                                                         cggACAATAAAGGTAAACATTTCTGCCACCACCAGGCCAATAATAATCCCGGAGATGATATTCTCCCCACCTAACCAGTTTGCACCAACCGCATAGGCCTCACCGACACTATATGGGGTGACGGTCATAAATGCTGCAAc                                                            <  1:405894/140‑1 (MQ=255)                                                                           gACAATAAAGGTAAACATTTCTGCCACCACCAGGCCAATAATAATCCCGGAGATGATATTCTCCCCACCTAACCAGTTTGCACCAACCGCATAGGCCTCACCGACACTATATGGGGTGACGGTCATAAATGCTGCAACgg                                                          >  1:469159/1‑140 (MQ=255)                                                                                              tCTGCCACCACCAGGCCAATAATAATCCCGGAGATGATATTCTCCCCACCTAACCAGTTTGCACCAACCGCATAGGCCTCACCGACACTATATGGGGTGACGGTCATAAATGCTGCAACGGATAACAACCCAGCCGCCAg                                       >  2:584513/1‑140 (MQ=255)                                                                                                       accaGGCCAATAATAATCCCGGAGATGATATTCTCCCCACCTAACCAGTTTGCACCAACCGCATAGGCCTCACCGACACTATATGGGGTGACGGTCATAAATGCTGCAACGGATAACAACCCAGCCGCCAGCGCATCGAc                              >  1:392391/1‑140 (MQ=255)                                                                                                            gCCAATAATAATCCCGGAGATGATATTCTCCCCACCTAACCAGTTTGCACCAACCGCATAGGCCTCACCGACACTATATGGGGTGACGGTCATAAATGCTGCAACGGATAACAACCCAGCCGCCAGCGCATCGACTTTAc                         >  2:447208/1‑140 (MQ=255)                                                                                                                ataataatCCCGGAGATGATATTCTCCCCACCTAACCAGTTTGCACCAACCGCATAGGCCTCACCGACACTATATGGGGTGACGGTCATAAATGCTGCAACGGATAACAACCCAGCCGCCAGCGCATCGACTTTACGctc                     >  2:104450/1‑140 (MQ=255)                                                                                                                  aataatCCCGGAGATGATATTCTCCCCACCTAACCAGTTTGCACCAACCGCATAGGCCTCACCGACACTATATGGGGTGACGGTCATAAATGCTGCAACGGATAACAACCCAGCCGCCAGCGCATCGACTTTACGCtctt                   <  1:626984/140‑1 (MQ=255)                                                                                                                      atCCCGGAGATGATATTCTCCCCACCTAACCAGTTTGCACCAACCGCATAGGCCTCACCGACACTATATGGGGTGACGGTCATAAATGCTGCAACGGATAACAACCCAGCCGCCAGCGCATCGACTTTACGCTCTTCTGc               >  2:199061/1‑140 (MQ=255)                                                                                                                       tCCCGGAGATGATATTCTCCCCACCTAACCAGTTTGCACCAACCGCATAGGCCTCACCGACACTATATGGGGTGACGGTCATAAATGCTGCAACGGATAACAACCCAGCCGCCAGCGCATCGACTTTACGCTCTTCTGcc              >  1:756187/1‑140 (MQ=255)                                                                                                                            gagaTGATATTCTCCCCACCTAACCAGTTTGCACCAACCGCATAGGCCTCACCGACACTATATGGGGTGACGGTCATAAATGCTGCAACGGATAACAACCCAGCCGCCAGCGCATCGACTTTACGCTCTTCTGCCAgcgc         <  2:658631/140‑1 (MQ=255)                                                                                                                                 gatATTCTCCCCACCTAACCAGTTTGCACCAACCGCATAGGCCTCACCGACACTATATGGGGTGACGGTCATAAATGCTGCAACGGATAACAACCCAGCCGCCAGCGCATCGACTTTACGCTCTTCTGCCAGCGCCATGc    <  1:584513/140‑1 (MQ=255)                                                                                                                                 gatATTCTCCCCACCTAACCAGTTTGCACCAACCGCATAGGCCTCACCGACACTATATGGGGTGACGGTCATAAATGCTGCAACGGATAACAACCCAGCCGCCAGCGCATCGACTTTACGCTCTTCTGCCAGCGCCATGc    <  1:447208/140‑1 (MQ=255)                                                                                                                                   tatTCTCCCCACCTAACCAGTTTGCACCAACCGCATAGGCCTCACCGACACTATATGGGGTGACGGTCATAAATGCTGCAACGGATAACAACCCAGCCGCCAGCGCATCGACTTTACGCTCTTCTGCCAGCGCCATGCCa  <  2:469159/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       GGAATTAATGCCGAGAAGGAACGCGATACTGAAGCAGGTACGCTGTCGGGCAGTTTAATGACCCAATTGCGGCGGACAATAAAGGTAAACATTTCTGCCACCACCAGGCCAATAATAATCCCGGAGATGATATTCGCCCCACCTAACCAGTTTGCACCAACCGCATAGGCCTCACCGACACTATATGGGGTGACGGTCATAAATGCTGCAACGGATAACAACCCAGCCGCCAGCGCATCGACTTTACGCTCTTCTGCCAGCGCCATGCCA  >  NC_000913/1820640‑1820909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑