| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 646,747 | 0 | T | G | 29.6% | 44.6 / 15.1 | 27 | T255P (ACC→CCC) | citG | triphosphoribosyl‑dephospho‑CoA synthase |
| Reads supporting (aligned to +/- strand): ref base T (0/19); new base G (8/0); total (8/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.50e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAG > NC_000913/646619‑646887 | gTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑Gcccc < 2:516017/140‑1 (MQ=255) ttAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCttt < 1:95278/140‑1 (MQ=255) tAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCtttt < 1:418634/140‑1 (MQ=255) atTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGGCGGAT‑GCCCCCctttttgaaa > 2:295074/1‑135 (MQ=255) ttAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCaata < 2:687293/140‑1 (MQ=255) tAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTTGCATATCCCCCCTTTTgataaa > 1:752596/1‑135 (MQ=255) tAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCaataa < 1:548814/140‑1 (MQ=255) tgtgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGAT‑GCCCCCCTTTTTCCATAAAGtttt > 2:75132/1‑139 (MQ=255) gtgatAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAAT‑GCCCCCCTTTTTCAATAAAGttttc > 1:524743/5‑138 (MQ=255) tgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCTAAT‑GGCCCCCTTTTTTAATAATAtttttg > 2:211086/1‑137 (MQ=255) tgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAAT‑GCCCCCCTTTTTTCATAATttttttg > 2:207281/1‑137 (MQ=255) aaaaaCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTc < 1:448730/140‑1 (MQ=255) aaaaaCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTc < 1:527074/140‑1 (MQ=255) aaaaCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGAT‑GCCCCCCTTTTTCAATAATTTTTTCGccccc > 2:577363/1‑137 (MQ=255) tAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGc < 1:207281/140‑1 (MQ=255) tCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAAT‑GCCCCCCTTTTTCAATAATGTTTTCGCCTCGCGCCGTAGCCAgcgc > 1:488775/1‑140 (MQ=255) gTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGAAT‑GCCCCCCTTTTTCAATAATGTTTGCGCCTCCCGCCGTAGGCAGCGCAgg > 1:32763/1‑140 (MQ=255) gTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAAA‑GCCCCCCTTTTTCAATAATTTTTTCGCCCCCCGCCGTAGCCAGCGCCgg > 1:189611/1‑140 (MQ=255) tAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAAT‑GCCCCCCTTTTTTCATAATTTTTTCGCCCCCCGCCGTAGCCAGCGCCGGc > 2:74983/1‑140 (MQ=255) cAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGCAG‑GAACCCCTTTTTCTATAAAATTTGTGCCTCGCCCCGCTGCCAGCCCACGcccccc > 2:176942/1‑140 (MQ=255) cAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGGT‑GCCCCCCTTTTTGGATAAAGTTTGGCGCTCGCGCCGTAAGCAGCGCAGGgccccc > 2:580872/1‑140 (MQ=255) cAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGAT‑GCCCCCCTTTTTCTATAATGTTTTCGCCCCCCCCCGTAGCCCGCGCCGGcgcccc > 2:687660/1‑140 (MQ=255) aGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCt < 2:677544/140‑1 (MQ=255) ccgccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAAA‑AACCCCCTTTTTCAATAAAATTTGTGCCTCGCCCTTTTGGCCGCGCCGGCCCCCCCCGCCAcc > 2:576628/1‑140 (MQ=255) gccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACcgc < 1:576628/140‑1 (MQ=255) ccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACcgcg < 2:143794/140‑1 (MQ=255) ccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAAT‑GCCCCCTTTTT‑CAATAATGTTTTCGCCTCCCGCTGTAGCCAGCGCAGGCCCCCCCCCCCACcccgc > 1:414392/1‑140 (MQ=255) gttcgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTa < 1:445778/140‑1 (MQ=255) tGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGcc < 1:442171/140‑1 (MQ=255) tCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCAt < 1:40025/140‑1 (MQ=255) aCTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGt < 1:74983/140‑1 (MQ=255) tGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAg < 2:162410/140‑1 (MQ=255) tGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTATAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAg < 2:752596/140‑1 (MQ=255) gagaTAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCa < 1:580872/140‑1 (MQ=255) gcggGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGc < 1:402803/140‑1 (MQ=255) ttCGAAT‑G‑CCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAg < 2:414392/140‑1 (MQ=255) | GTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAT‑GCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAG > NC_000913/646619‑646887 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |