Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	193,169	G→T	V100F (GTT→TTT)	frr →	ribosome‑recycling factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	193,169	0	G	T	100.0%	148.8 / NA	43	V100F (GTT→TTT)	frr	ribosome‑recycling factor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (19/24);  total (19/24)

CAAGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGATTATGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTGTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCGTGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAG  >  NC_000913/193035‑193304                                                                                                                                       |                                                                                                                                        cAAGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGATTATGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTTTTCCg                                                                                                                                    >  2:376288/1‑140 (MQ=255)    gCGTAACGGTAGAAGATTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGATTATGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTTTTCCGCTg                                                                                                                                 <  2:93478/140‑1 (MQ=255)       tAACGGTAGAAGATTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGATTATGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTTTTCCGCTgccg                                                                                                                              <  2:11599/140‑1 (MQ=255)          cGGTAGAAGATTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGATTATGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTTTTCCGCTGccgccg                                                                                                                           <  1:295056/140‑1 (MQ=255)              agaagaTTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGATTATGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTTTTCCGCTGCCGCCGCTGa                                                                                                                       <  1:425774/140‑1 (MQ=255)              agaagaTTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGATTATGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTTTTCCGCTGCCGCCGCTGa                                                                                                                       <  1:488454/140‑1 (MQ=255)                     tCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGATTATGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTTTTCCGCTGCCGCCGCTGACGgaaga                                                                                                                >  2:645396/1‑140 (MQ=255)                      cccGTACACTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGATTATGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTTTTCCGCTGCCGCCGCTGACGgaagaa                                                                                                               <  1:267787/140‑1 (MQ=255)                      cccGTACACTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGATTATGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTTTTCCGCTGCCGCCGCTGACGgaagaa                                                                                                               <  2:665579/140‑1 (MQ=255)                       ccGTACACTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGATTATGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTTTTCCGCTGCCGCCGCTGACGGAAGAAc                                                                                                              >  1:305110/1‑140 (MQ=255)                       ccGTACACTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGATTATGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTTTTCCGCTGCCGCCGCTGACGGAAGAAc                                                                                                              >  2:439283/1‑140 (MQ=255)                             acTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGATTATGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTTTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTa                                                                                                        <  2:693440/140‑1 (MQ=255)                               tGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGATTATGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTTTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTaaa                                                                                                      >  1:46015/1‑140 (MQ=255)                               tGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGATTATGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTTTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTaaa                                                                                                      >  2:157493/1‑140 (MQ=255)                                 aaaaTCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGATTATGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTTTTCCGCTGCCGCCGCTGACGGAAGAAc                                                                                                              >  1:275353/1‑130 (MQ=255)                                 aaaaTCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGATTATGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTTTTCCGCTGCCGCCGCTGACGGAAGAAc                                                                                                              <  2:275353/130‑1 (MQ=255)                                         cGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGATTATGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTTTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCa                                                                                            >  1:701401/1‑140 (MQ=255)                                                       cAATGTCTCCGGCCGTTGAAAAAGCGATTATGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTTTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCgtggt                                                                              >  2:3603/1‑140 (MQ=255)                                                        aaTGTCTCCGGCCGTTGAAAAAGCGATTATGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTTTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCgtggtg                                                                             >  2:154249/1‑140 (MQ=255)                                                           gTCTCCGGCCGTTGAAAAAGCGATTATGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTTTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCGTGGTGAAg                                                                          >  1:561647/1‑140 (MQ=255)                                                                  gCCGTTGAAAAAGCGATTATGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTTTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCGTGGTGAATCAGAACa                                                                   >  1:761171/1‑140 (MQ=255)                                                                   ccGTTGAAAAAGCGATTATGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTTTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCGTGGTGAAGCAGAACaa                                                                  <  1:498002/140‑1 (MQ=255)                                                                      ttGAAAAAGCGATTATGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTTTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCGTGGTGAAGCAGAACAAgcg                                                               >  2:545360/1‑140 (MQ=255)                                                                          aaaaGCGATTATGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTTTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCGTGGTGAAGCAGAACAAGCGCgtg                                                           <  2:380949/140‑1 (MQ=255)                                                                             aGCGATTATGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTTTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCGTGGTGAAGCAGAACAAGCGCGTGTTg                                                        <  2:502371/140‑1 (MQ=255)                                                                              gCGATTATGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTTTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCGTGGTGAAGCAGAACAAGCGCGTGTTGc                                                       <  1:645396/140‑1 (MQ=255)                                                                              gCGATTATGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTTTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCGTGGTGAAGCAGAACAAGCGCGTGTTGc                                                       <  2:712988/140‑1 (MQ=255)                                                                               cGATTATGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTTTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCGTGGTGAAGCAGAACAAGCGCGTGTTGCa                                                      <  1:513090/140‑1 (MQ=255)                                                                                                    gCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTTTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCGTGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGcgtcgt                                    <  1:246753/137‑1 (MQ=255)                                                                                                    gCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTTTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCGTGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGcgtcgt                                    >  2:246753/1‑137 (MQ=255)                                                                                                    gCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTTTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCGTGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGAc                                 >  1:606791/1‑140 (MQ=255)                                                                                                         aaCCCGAACTCTGCGGGTAGCGACATCCGTTTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCGTGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGaa                            >  2:65906/1‑140 (MQ=255)                                                                                                         aaCCCGAACTCTGCGGGTAGCGACATCCGTTTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCGTGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGaa                            >  2:390768/1‑140 (MQ=255)                                                                                                             cGAACTCTGCGGGTAGCGACATCCGTTTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCGTGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGAc                        <  1:3603/140‑1 (MQ=255)                                                                                                                      cGGGTAGCGACATCCGTTTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCGTGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGaaa               >  2:563783/1‑140 (MQ=255)                                                                                                                        ggTAGCGACATCCGTTTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCGTGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGc             <  2:310418/140‑1 (MQ=255)                                                                                                                        ggTAGCGACATCCGTTTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCGTGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGc             <  1:661672/140‑1 (MQ=255)                                                                                                                         gTAGCGACATCCGTTTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCGTGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCa            <  1:157493/140‑1 (MQ=255)                                                                                                                         gTAGCGACATCCGTTTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCGTGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCa            <  1:376288/140‑1 (MQ=255)                                                                                                                                cATCCGTTTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCGTGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGa     <  2:708432/140‑1 (MQ=255)                                                                                                                                  tCCGTTTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCGTGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGtt       >  2:126606/1‑136 (MQ=255)                                                                                                                                  tCCGTTTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCGTGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGtt       <  1:126606/136‑1 (MQ=255)                                                                                                                                   ccGTTTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCGTGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAg  <  2:406775/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        CAAGCGTAACGGTAGAAGATTCCCGTACACTGAAAATCAACGTGTTTGATCGTTCAATGTCTCCGGCCGTTGAAAAAGCGATTATGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTGTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCGTGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAG  >  NC_000913/193035‑193304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑