Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,542,685	C→A	D282Y (GAC→TAC)	cysP ←	thiosulfate/sulfate ABC transporter periplasmic binding protein CysP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,542,685	0	C	A	100.0%	93.0 / NA	27	D282Y (GAC→TAC)	cysP	thiosulfate/sulfate ABC transporter periplasmic binding protein CysP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (11/16);  total (11/16)

CGCCGCTGGTGAAGTGGGTTTTCATCACTTCCGGCCAGGAGCCAAATTTGTCTTCCACGCGGAACAGCTCGGTCTGCGGGAATTTGTCTTTCAGTTTGTCCATCACCTCCGGGTTATTCACGCGGTAGTAATAGTCGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTTCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCCGCCAGAATGTTGGTTTTCGGAAT  >  NC_000913/2542550‑2542820                                                                                                                                        |                                                                                                                                        cgccgcTGGTGAAGTGGGTTTTCATCACTTCCGGCCAGGAGCCAAATTTGTCTTCCACGCGGAACAGCTCGGTCTGCGGGAATTTGTCTTTCAGTTTGTCCATCACCTCCGGGTTATTCACGCGGTAGTAATAGTAGGtg                                                                                                                                     >  2:191260/1‑140 (MQ=255) cgccgcTGGTGAAGTGGGTTTTCATCACTTCCGGCCAGGAGCCAAATTTGTCTTCCACGCGGAACAGCTCGGTCTGCGGGAATTTGTCTTTCAGTTTGTCCATCACCTCCGGGTTATTCACGCGGTAGTAATAGTAGGtg                                                                                                                                     >  1:572717/1‑140 (MQ=255) cgccgcCGGTGAAGGGGGTTTTCATCACTTCCGGCCAGGCGCCAAATTTGTCTTCAACGCGGAACAGCTCGGTCTGAGGGAATTTGTCTTTCAGTTTGTCCATCACCTCCGGGTTATTCACGCGGGAGTAATAGTAGGtg                                                                                                                                     >  2:325406/1‑140 (MQ=255)     gcTGGTGAAGTGGGTTTTCATCACTTCCGGCCAGGAGCCAAATTTGTCTTCCACGCGGAACAGCTCGGTCTGCGGGAATTTGTCTTTCAGTTTGTCCATCACCTCCGGGTTATTCACGCGGTAGTAATAGTAGGtgatga                                                                                                                                 <  1:191260/140‑1 (MQ=255)                 ggTTTTCATCACTTCCGGCCAGGAGCCAAATTTGTCTTCCACGCGGAACAGCTCGGTCTGCGGGAATTTGTCTTTCAGTTTGTCCATCACCTCCGGGTTATTCACGCGGTAGTAATAGTAGGTGATGATGGTTTGCGCCt                                                                                                                     <  2:116497/140‑1 (MQ=255)                 ggTTTTCATCACTTCCGGCCAGGAGCCAAATTTGTCTTCCACGCGGAACAGCTCGGTCTGCGGGAATTTGTCTTTCAGTTTGTCCATCACCTCCGGGTTATTCACGCGGTAGTAATAGTAGGTGATGATGGTTTGCGCCt                                                                                                                     <  1:397071/140‑1 (MQ=255)                 ggTTTTCATCACTTCCGGCCAGGAGCCAAATTTGTCTTCCACGCGGAACAGCTCGGTCTGCGGGAATTTGTCTTTCAGTTTGTCCATCACCTCCGGGTTATTCACGCGGTAGTAATAGTAGGTGATGATGGTTTGCGCCt                                                                                                                     <  1:708348/140‑1 (MQ=255)                    tttCATCACTTCCGGCCAGGAGCCAAATTTGTCTTCCACGCGGAACAGCTCGGTCTGCGGGAATTTGTCTTTCAGTTTGTCCATCACCTCCGGGTTATTCACGCGGTAGTAATAGTAGGTGATGATGGTTTGCGCCTGCg                                                                                                                  <  2:291405/140‑1 (MQ=255)                    tttCATCACTTCCGGCCAGGAGCCAAATTTGTCTTCCACGCGGAACAGCTCGGTCTGCGGGAATTTGTCTTTCAGTTTGTCCATCACCTCCGGGTTATTCACGCGGTAGTAATAGTAGGTGATGATGGTTTGCGCCTGCg                                                                                                                  <  1:664525/140‑1 (MQ=255)                     ttCATCACTTCCGGCCAGGAGCCAAATTTGTCTTCCACGCGGAACAGCTCGGTCTGCGGGAATTTGTCTTTCAGTTTGTCCATCACCTCCGGGTTATTCACGCGGTAGTAATAGTAGGTGATGATGGTTTGCGCCTGCgg                                                                                                                 <  1:640379/140‑1 (MQ=255)                          caCTTCCGGCCAGGAGCCAAATTTGTCTTCCACGCGGAACAGCTCGGTCTGCGGGAATTTGTCTTTCAGTTTGTCCATCACCTCCGGGTTATTCACGCGGTAGTAATAGTAGGTGATGATGGTTTGCGCCTGCGGGCtat                                                                                                            <  1:209815/140‑1 (MQ=255)                           aCTTCCGGCCAGGAGCCAAATTTGTCTTCCACGCGGAACAGCTCGGTCTGCGGGAATTTGTCTTTCAGTTTGTCCATCACCTCCGGGTTATTCACGCGGTAGTAATAGTAGGTGATGATGGTTTGCGCCTGCg                                                                                                                  >  1:266411/1‑133 (MQ=255)                           aCTTCCGGCCAGGAGCCAAATTTGTCTTCCACGCGGAACAGCTCGGTCTGCGGGAATTTGTCTTTCAGTTTGTCCATCACCTCCGGGTTATTCACGCGGTAGTAATAGTAGGTGATGATGGTTTGCGCCTGCg                                                                                                                  <  2:266411/133‑1 (MQ=255)                                 ggCCAGGAGCCAAATTTGTCTTCCACGCGGAACAGCTCGGTCTGCGGGAATTTGTCTTTCAGTTTGTCCATCACCTCCGGGTTATTCACGCGGTAGTAATAGTAGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCa                                                                                                     >  1:399831/1‑140 (MQ=255)                                       gAGCCAAATTTGTCTTCCACGCGGAACAGCTCGGTCTGCGGGAATTTGTCTTTCAGTTTGTCCATCACCTCCGGGTTATTCACGCGGTAGTAATAGTAGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAg                                                                                               >  2:214773/1‑140 (MQ=255)                                        aGCCAAATTTGTCTTCCACGCGGAACAGCTCGGTCTGCGGGAATTTGTCTTTCAGTTTGTCCATCACCTCCGGGTTATTCACGCGGTAGTAATAGTAGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGa                                                                                              <  1:772151/140‑1 (MQ=255)                                        aGCCAAATTTGTCTTCCACGCGGAACAGCTCGGTCTGCGGGAATTTGTCTTTCAGTTTGTCCATCACCTCCGGGTTATTCACGCGGTAGTAATAGTAGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGa                                                                                              <  2:619637/140‑1 (MQ=255)                                                             ggAACAGCTCGGTCTGCGGGAATTTGTCTTTCAGTTTGTCCATCACCTCCGGGTTATTCACGCGGTAGTAATAGTAGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTTcc                                                                         >  1:195323/1‑140 (MQ=255)                                                                aCAGCTCGGTCTGCGGGAATTTGTCTTTCAGTTTGTCCATCACCTCCGGGTTATTCACGCGGTAGTAATAGTAGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTTCCGTa                                                                      <  1:253756/140‑1 (MQ=255)                                                                        gTCTGCGGGAATTTGTCTTTCAGTTTGTCCATCACCTCCGGGTTATTCACGCGGTAGTAATAGTAGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTTCCGTACCGTTGGc                                                              <  1:214773/140‑1 (MQ=255)                                                                                gAATTTGTCTTTCAGTTTGTCCATCACCTCCGGGTTATTCACGCGGTAGTAATAGTAGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTTCCGTACCGTTGGCCTGCACGt                                                      <  2:399831/140‑1 (MQ=255)                                                                                               tttGTCCATCACCTCCGGGTTATTCACGCGGTAGTAATAGTAGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTTCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACg                                       <  2:416955/140‑1 (MQ=255)                                                                                                                     ttCACGCGGTAGTAATAGTAGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTTCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCCGCCa                     >  1:734188/1‑136 (MQ=255)                                                                                                                     ttCACGCGGTAGTAATAGTAGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTTCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCCGCCa                     <  2:734188/136‑1 (MQ=255)                                                                                                                     ttCACGCGGTAGTAATAGTAGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTTCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCCGCCAGAAt                 >  2:321879/1‑140 (MQ=255)                                                                                                                      tCACGCGGTAGTAATAGTAGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTTCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCCGCCAGAATg                >  2:531678/1‑140 (MQ=255)                                                                                                                          gcgGTAGTAATAGTAGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTTCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCCGCCAGAATGTTgg            >  2:53128/1‑140 (MQ=255)                                                                                                                            ggTAGTAATAGTAGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTTCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCCGCCAGAATGTTGGtt          >  2:399284/1‑140 (MQ=255)                                                                                                                                    tagtagGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTTCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCCGCCAGAATGTTGGTTTTCGGAAt  <  2:583081/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        CGCCGCTGGTGAAGTGGGTTTTCATCACTTCCGGCCAGGAGCCAAATTTGTCTTCCACGCGGAACAGCTCGGTCTGCGGGAATTTGTCTTTCAGTTTGTCCATCACCTCCGGGTTATTCACGCGGTAGTAATAGTCGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTTCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCCGCCAGAATGTTGGTTTTCGGAAT  >  NC_000913/2542550‑2542820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑