Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,341,141	C→A	R163L (CGA→CTA)	melR ←	DNA‑binding transcriptional dual regulator MelR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,341,141	0	C	A	100.0%	244.4 / NA	70	R163L (CGA→CTA)	melR	DNA‑binding transcriptional dual regulator MelR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (39/31);  total (39/31)

CAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGCCAGCGGCGCACTTCAAACGGGCTAAGTTGCTGTGTGGCCAGTGATTTGATCACCATGCCG  >  NC_000913/4341005‑4341275                                                                                                                                         |                                                                                                                                       cAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCt                                                                                                                                     >  1:105883/1‑140 (MQ=255)   gcgcCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTg                                                                                                                                   <  1:183438/140‑1 (MQ=255)    cgcCTGATCATAGTTTTCGGCAATAAATCCCAGCATCTTGGTAACATAAAATTGCGCATGGCGCGAGACGCTGCTTTTGTGATTGCGCGAGGCTTTATTGACCAGACTCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGa                                                                                                                                  >  2:291664/1‑140 (MQ=255)         gATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATc                                                                                                                             <  2:361359/140‑1 (MQ=255)            cATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAgg                                                                                                                          <  2:529047/140‑1 (MQ=255)             aTAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGc                                                                                                                         <  2:735554/140‑1 (MQ=255)                 ttttCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAAt                                                                                                                     >  1:208513/1‑140 (MQ=255)                 ttttCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAAt                                                                                                                     <  1:44681/140‑1 (MQ=255)                    tCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAAt                                                                                                                     <  2:638298/137‑1 (MQ=255)                    tCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAAt                                                                                                                     >  1:638298/1‑137 (MQ=255)                     cGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTtca                                                                                                                 >  1:52697/1‑140 (MQ=255)                           tAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATg                                                                                                           >  1:680928/1‑140 (MQ=255)                                 ccAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGc                                                                                                     >  1:354731/1‑140 (MQ=255)                                     cATCTGGCTAACATAAAATTGCGCATGGTGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGc                                                                                                 >  2:262033/1‑140 (MQ=255)                                       tCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGa                                                                                               >  2:18448/1‑140 (MQ=255)                                        cTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTATGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGaa                                                                                              >  1:50104/1‑140 (MQ=255)                                         tGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAAt                                                                                             >  2:432511/1‑140 (MQ=255)                                             tAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGc                                                                                         >  2:344599/1‑140 (MQ=255)                                              aaCATAAAATTGCGCATGGTGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCt                                                                                        <  1:262033/140‑1 (MQ=255)                                                 aTAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGt                                                                                     >  2:249119/1‑140 (MQ=255)                                                  tAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGtt                                                                                    <  1:689802/140‑1 (MQ=255)                                                    aaaTTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCg                                                                                  <  1:130575/140‑1 (MQ=255)                                                     aaTTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCgg                                                                                 <  2:208513/140‑1 (MQ=255)                                                      aTTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGa                                                                                <  1:117679/140‑1 (MQ=255)                                                      aTTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGa                                                                                <  1:564968/140‑1 (MQ=255)                                                      aTTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGa                                                                                <  2:533561/140‑1 (MQ=255)                                                      aTTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGa                                                                                <  1:432511/140‑1 (MQ=255)                                                       ttGCGCATGGCGCGAGACGCTGCTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGAc                                                                               <  2:441879/140‑1 (MQ=255)                                                          cgcATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGt                                                                            >  1:250621/1‑140 (MQ=255)                                                            cATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGttt                                                                          <  2:553312/140‑1 (MQ=255)                                                               ggCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAAt                                                                       >  1:33854/1‑140 (MQ=255)                                                                 cgcgAGTCGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGTCCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTc                                                                     >  2:532110/1‑140 (MQ=255)                                                                         gCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGcc                                                             >  1:170514/1‑140 (MQ=255)                                                                         gCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGcc                                                             >  1:317977/1‑140 (MQ=255)                                                                           tGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGCCAg                                                           >  1:437782/1‑140 (MQ=255)                                                                                     tgtgCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGCCAGCGGCGCACtt                                                 >  2:759449/1‑140 (MQ=255)                                                                                     tgtgCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGCCAGCGGCGCACtt                                                 >  1:439700/1‑140 (MQ=255)                                                                                         cgcgAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGCCAgcgg                                                        >  1:484536/1‑129 (MQ=255)                                                                                         cgcgAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGCCAgcgg                                                        <  2:484536/129‑1 (MQ=255)                                                                                         cgcgAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGCCAGCGGCGCACTTCaaa                                             >  1:95679/1‑140 (MQ=255)                                                                                         cgcgAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGCCAGCGGCGCACTTCaaa                                             >  1:407595/1‑140 (MQ=255)                                                                                         cgcgAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGCCAGCGGCGCACTTCaaa                                             >  1:671572/1‑140 (MQ=255)                                                                                               gTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGCCAGCGGCGCACTTCAAACGGGCt                                       >  2:242656/1‑140 (MQ=255)                                                                                               gTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGCCAGCGGCGCACTTCAAACGGGCt                                       <  2:337794/140‑1 (MQ=255)                                                                                                    aTTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGCCAGCGGCGCACTTCAAACGGGCTAAGtt                                  <  2:170514/140‑1 (MQ=255)                                                                                                       gACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGCCAGCGGCGCACTTCAAACGGGCTAAGTTGCt                               <  2:673843/140‑1 (MQ=255)                                                                                                        aCCAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGCCAGCGGCGCACTTCAAACGGGCTAAGTTGCtg                              >  1:622198/1‑140 (MQ=255)                                                                                                          cAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGCCAGCGGCGCACTTCAAACGGGCTAAg                                    <  1:211275/132‑1 (MQ=255)                                                                                                          cAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGCCAGCGGCGCACTTCAAACGGGCTAAg                                    >  2:211275/1‑132 (MQ=255)                                                                                                          cAGAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGCCAGCGGCGCACTTCAAACGGGCTAAGTTGCtgtg                            >  2:430268/1‑140 (MQ=255)                                                                                                            gAATCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGCCAGCGGCGCACTTCAAACGGGCTAAGTTGCtgtgtg                          >  2:410635/1‑140 (MQ=255)                                                                                                             aaTCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGCCAGCGGCGCACTTCAAACGGGCTAAGTTGCTGTGTgg                         >  1:692367/1‑140 (MQ=255)                                                                                                             aaTCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGCCAGCGGCGCACTTCAAACGGGCTAAGTTGCTGTGTgg                         >  1:685290/1‑140 (MQ=255)                                                                                                              aTCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGCCAGCGGCGCACTTCAAACGGGCTAAGTTGCTGTGTGGc                        <  1:291664/140‑1 (MQ=255)                                                                                                               tCGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGCCAGCGGCGCACTTCAAACGGGCTAAGTTGCTGTGTGGcc                       >  2:588698/1‑140 (MQ=255)                                                                                                                cGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGCCAGCGGCGCACTTCAAACGGGCTAAGTTGCTGTGTGGCCa                      <  2:775695/140‑1 (MQ=255)                                                                                                                cGGTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGCCAGCGGCGCACTTCAAACGGGCTAAGTTGCTGTGTGGCCa                      <  1:194774/140‑1 (MQ=255)                                                                                                                  gTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGCCAGCGGCGCACTTCAAACGGGCTAAGTTGCTGTGTGGCCAGt                    <  1:242656/140‑1 (MQ=255)                                                                                                                  gTTCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGCCAGCGGCGCACTTCAAACGGGCTAAGTTGCTGTGTGGCCAGt                    >  1:798458/1‑140 (MQ=255)                                                                                                                   ttCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGCCAGCGGCGCACTTCAAACGGGCTAAGTTGCTGTGTGGCCAGTg                   <  2:95679/140‑1 (MQ=255)                                                                                                                   ttCCCAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGCCAGCGGCGCACTTCAAACGGGCTAAGTTGCTGTGTGGCCAGTg                   <  1:514127/140‑1 (MQ=255)                                                                                                                     cccAGCCAGAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGCCAGCGGCGCACTTCAAACGGGCTAAGTTGCTGTGTGGCCAGTGAt                 <  2:671572/140‑1 (MQ=255)                                                                                                                       cagccagAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGCCAGCGGCGCACTTCAAACGGGCTAAGTTGCTGTGTGGCCAGTGAttt               >  1:58052/1‑140 (MQ=255)                                                                                                                       cagccagAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGCCAGCGGCACACTTCAAACGGGCTAAGTTGCTGTGTGGCCAGTGAttt               >  1:613432/1‑140 (MQ=255)                                                                                                                         gccagAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGCCAGCGGCGCACTTCAAACGGGCTAAGTTGCTGTGTGGCCAGTGATTTGa             <  1:18448/140‑1 (MQ=255)                                                                                                                         gccagAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGCCAGCGGCGCACTTCAAACGGGCTAAGTTGCTGTGTGGCCAGTGATTTGa             <  2:714715/140‑1 (MQ=255)                                                                                                                          ccagAGAGGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGCCAGCGGCGCACTTCAAACGGGCTAAGTTGCTGTGTGGCCAGTGATTTGAt            >  1:267267/1‑140 (MQ=255)                                                                                                                             gagagGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGCCAGCGGCGCACTTCAAACGGGCTAAGTTGCTGTGTGGCCAGTGATTTGATCAc         >  2:549241/1‑140 (MQ=255)                                                                                                                              agagGCTAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGCCAGCGGCGCACTTCAAACGGGCTAAGTTGCTGTGTGGCCAGTGATTTGATCAcc        <  1:557297/140‑1 (MQ=255)                                                                                                                                    tAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGCCAGCGGCGCACTTCAAACGGGCTAAGTTGCTGTGTGGCCAGTGATTTGATCACCATGCCg  >  1:42008/1‑140 (MQ=255)                                                                                                                                    tAAATAGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGCCAGCGGCGCACTTCAAACGGGCTAAGTTGCTGTGTGGCCAGTGATTTGATCACCATGCCg  >  1:350513/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       CAGCGCCTGATCATAGTTTTCGGCAATAAAGCCCAGCATCTGGCTAACATAAAATTGCGCATGGCGCGAGACGCTGTTTTTGTGTGTGCGCGAGGTTTTATTGACCAGAATCGGTTCCCAGCCAGAGAGGCTAAATCGCTTGAGCATCAGGCCAATTTCATCAATGGCGAGCTGGCGAATTTGCTCGTTCGGACTGTTTAATTCCTGCTGCCAGCGGCGCACTTCAAACGGGCTAAGTTGCTGTGTGGCCAGTGATTTGATCACCATGCCG  >  NC_000913/4341005‑4341275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑