| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,418,675 | C→A | V317V (GTG→GTT) | ulaG ← | L‑ascorbate‑6‑phosphate lactonase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,418,675 | 0 | C | A | 98.2% | 190.8 / ‑4.1 | 57 | V317V (GTG→GTT) | ulaG | L‑ascorbate‑6‑phosphate lactonase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (34/22); minor base G (0/1); total (34/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01 | |||||||||||
CTACCCGGTATAAACGACAGATTACAGGAATGACTTGAACGGCAGATCCGGTTCAATAGTGAAGCAATCATCGAAACCGCGCGGATAGTGGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCCACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCA > NC_000913/4418540‑4418811 | cTACCCGGTATAAACGACAGATTACAGGAATGACTTGAACGGCAGATCCGGTTCAATAGTGAAGCAATCATCGAAACCGCGCGGATAGTGGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCt < 1:433859/140‑1 (MQ=255)cTACCCGGTATAAACGACAGATTACAGGAATGACTTGAACGGCAGATCCGGTTCAATAGTGAAGCAATCATCGAAACCGCGCGGATAGTGGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCt < 1:547049/140‑1 (MQ=255) cccGGTATAAACGACAGATTACAGGAATGACTTGAACGGCAGATCCGGTTCAATAGTGAAGCAATCATCGAAACCGCGCGGATAGTGGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGcc > 1:699013/1‑140 (MQ=255) cccGGTATAAACGACAGATTACAGGAATGACTTGAACGGCAGATCCGGTTCAATAGTGAAGCAATCATCGAAACCGCGCGGATAGTGGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGcc > 2:660655/1‑140 (MQ=255) ataAACGACAGATTACAGGAATGACTTGAACGGCAGATCCGGTTCAATAGTGAAGCAATCATCG‑AACCGCGCGGGTAGTGGTACCCGAAGTTGTCTTTATCCAACCGCCAGGGAAATTTGCCACCAACCTGCCAGATaaa > 2:129057/1‑140 (MQ=255) aCGACAGATTACAGGAATGACTTGAACGGCAGATCCGGTTCAATAGTGAAGCAATCATCGAAACCGCGCGGATAGTGGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACgg > 1:620386/1‑140 (MQ=255) aCGACAGATTACAGGAATGACTTGAACGGCAGATCCGGTTCAATAGTGAAGCAATCATCGAAACCGCGCGGATAGTGGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACgg > 1:443780/1‑140 (MQ=255) aGATTACAGGAATGACTTGAACGGCAGATCCGGTTCAATAGTGAAGCAATCATCGAAACCGCGCGGATAGTGGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGa > 1:198663/1‑140 (MQ=255) aGATTACAGGAATGACTTGAACGGCAGATCCGGTTCAATAGTGAAGCAATCATCGAAACCGCGCGGATAGTGGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGa < 2:33602/140‑1 (MQ=255) aGATTACAGGAATGACTTGAACGGCAGATCCGGTTCAATAGTGAAGCAATCATCGAAACCGCGCGGATAGTGGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGa > 1:684307/1‑140 (MQ=255) aTTACAGGAATGACTTGAACGGCAGATCCGGTTCAATAGTGAAGCAATCATCGAAACCGCGCGGATAGTGGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAg > 1:588939/1‑140 (MQ=255) tACAGGAATGACTTGAACGGCAGATCCGGTTCAATAGTGAAGCAATCATCGAAACCGCGCGGATAGTGGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGcc < 2:588939/140‑1 (MQ=255) tGACTTGAACGGCAGATCCGGTTCAATAGTGAAGCAATCATCGAAACCGCGCGGATAGTGGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCa > 2:400184/1‑140 (MQ=255) aCTTGAACGGCAGATCCGGTTCAATAGTGAAGCAATCATCGAAACCGCGCGGATAGTGGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAgg > 2:53193/1‑140 (MQ=255) cAGATCCGGTTCAATAGTGAAGCAATCATCGAAACCGCGCGGATAGTGGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCttttt < 1:531859/140‑1 (MQ=255) aTCCGGTTCAATAGTGAAGCAATCATCGAAACCGCGCGGATAGTGGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCa < 1:53193/140‑1 (MQ=255) aTCCGGTTCAATAGTGAAGCAATCATCGAAACCGCGCGGATAGTGGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCa < 2:625992/140‑1 (MQ=255) tCCGGTTCAATAGTGAAGCAATCATCGAAACCGCGCGGATAGTGGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCGACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCAt < 1:294482/140‑1 (MQ=255) tCAATAGTGAAGCAATCATCGAAACCGCGCGGATAGTGGTACTTGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCca < 2:718324/140‑1 (MQ=255) tCAATAGTGAAGCAATCATCGAAACCGCGCGGATAGTGGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCca < 1:54353/140‑1 (MQ=255) aTAGTGAAGCAATCATCGAAACCGCGCGGATAGTGGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAg < 1:776172/140‑1 (MQ=255) aaGCAATCATCGAAACCGCGCGGATAGTGGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCAcgcg > 1:693189/1‑140 (MQ=255) cAATCATCGAAACCGCGCGGATAGTGGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGAt > 1:623549/1‑140 (MQ=255) atcatcGAAACCGCGCGGATAGTGGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGAtct > 1:618407/1‑140 (MQ=255) atcatcGAAACCGCGCGGATAGTGGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGAtct > 1:218495/1‑140 (MQ=255) atcGAAACCGCGCGGATAGTGGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCtt > 2:386546/1‑140 (MQ=255) atcGAAACCGCGCGGATAGTGGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCtt > 1:495367/1‑140 (MQ=255) aCCGCGCGGATAGTGGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGAt < 2:420773/140‑1 (MQ=255) aCCGCGCGGATAGTGGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGAt < 1:421431/140‑1 (MQ=255) aCCGCGCGGATAGTGGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGAt < 1:527892/140‑1 (MQ=255) cgcgcgGATAGTGGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCg > 1:744212/1‑140 (MQ=255) cgcgGATAGTGGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGc > 2:659334/1‑140 (MQ=255) aTAGTGGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGCCCTGGa > 2:109140/1‑140 (MQ=255) gTGGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGt < 2:620386/140‑1 (MQ=255) tGGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGtt > 2:286115/1‑140 (MQ=255) gTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTg < 1:81788/140‑1 (MQ=255) gTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTg < 1:659334/140‑1 (MQ=255) tACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGa < 1:523102/140‑1 (MQ=255) cTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGAcc < 2:684307/140‑1 (MQ=255) tCGAAGTTGTCTTTATCCAGCGGCGAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCa > 1:597376/1‑140 (MQ=255) tCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCa > 2:75006/1‑140 (MQ=255) tCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCa > 1:236682/1‑140 (MQ=255) tCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCa > 1:603428/1‑140 (MQ=255) gAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGa > 2:640117/1‑140 (MQ=255) aGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGata > 1:564446/1‑140 (MQ=255) aGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGata > 1:732459/1‑140 (MQ=255) aGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGata > 1:672471/1‑140 (MQ=255) tGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCg > 2:386080/1‑140 (MQ=255) tCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCgtg > 2:147054/1‑140 (MQ=255) tCTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCgtg > 2:407886/1‑140 (MQ=255) cTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCgtgg < 2:198663/140‑1 (MQ=255) cTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCgtgg > 2:297764/1‑140 (MQ=255) cTTTATCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCgtgg < 1:75006/140‑1 (MQ=255) aTCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGa < 1:129057/140‑1 (MQ=255) tCCAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGaa < 2:612597/140‑1 (MQ=255) ccAGCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAAc < 1:407886/140‑1 (MQ=255) gCGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACggg > 2:430806/1‑140 (MQ=255) cGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGa > 1:91594/1‑140 (MQ=255) cGGCCAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGACCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGa > 1:562463/1‑140 (MQ=255) ccAGGTAAATTTGCCACCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCa > 2:66079/1‑140 (MQ=255) ccaccaacCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCAtt < 2:672471/140‑1 (MQ=255) accaaccTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCa < 2:443780/140‑1 (MQ=255) | CTACCCGGTATAAACGACAGATTACAGGAATGACTTGAACGGCAGATCCGGTTCAATAGTGAAGCAATCATCGAAACCGCGCGGATAGTGGTACTCGAAGTTGTCTTTATCCAGCGGCCAGGTAAATTTGCCACCCACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATCACTACTTTCGCATTCA > NC_000913/4418540‑4418811 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |