Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	807,640	G→T	G90G (GGC→GGA)	ybhB ←	putative kinase inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	807,640	0	G	T	100.0%	177.3 / NA	53	G90G (GGC→GGA)	ybhB	putative kinase inhibitor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (27/26);  total (27/26)

CTGGCACCTTCATCGACATCAATACGTTCTATATCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACGCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTAATACGCGGGTATCAGCGGGTAAGTTAACAACTACCCAGTGCCACCAGCCGGAGCCGGTTGGCGCATCCGGGTCGTAGCAGGTGAC  >  NC_000913/807506‑807768                                                                                                                                       |                                                                                                                                 cTGGCACCTTCATCGACATCAATACGTTCTATATCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTc                                                                                                                             >  2:354124/1‑140 (MQ=255)     cACCTTCATCGACATCAATACGTTCTATATCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGc                                                                                                                         <  1:647828/140‑1 (MQ=255)         ttCATCGACATCAATACGTTCTATATCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTg                                                                                                                     >  2:452213/1‑140 (MQ=255)              cGACATCAATACGTTCTATATCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTacc                                                                                                                <  1:657628/140‑1 (MQ=255)                  aTCAATACGTTCTATATCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTaccagac                                                                                                            <  2:732348/140‑1 (MQ=255)                   tCAATACGTTCTATATCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTaccagacc                                                                                                           <  2:251203/140‑1 (MQ=255)                    cAATACGTTCTATATCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTaccagacca                                                                                                          >  1:98285/1‑140 (MQ=255)                    cAATACGTTCTATATCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTaccagacca                                                                                                          >  2:10432/1‑140 (MQ=255)                          gTTCTATATCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCa                                                                                                    <  2:549765/140‑1 (MQ=255)                              tataTCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAAcc                                                                                                >  2:670444/1‑140 (MQ=255)                                 atCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCtt                                                                                             <  2:256535/140‑1 (MQ=255)                                 atCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCtt                                                                                             >  2:371665/1‑140 (MQ=255)                                 atCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCtt                                                                                             >  2:444693/1‑140 (MQ=255)                                  tCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTg                                                                                            <  1:140112/140‑1 (MQ=255)                                   ccAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGc                                                                                           >  2:948/1‑140 (MQ=255)                                    cAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCg                                                                                          >  1:310447/1‑140 (MQ=255)                                     aGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCgg                                                                                         >  1:611411/1‑140 (MQ=255)                                     aGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCgg                                                                                         >  1:591039/1‑140 (MQ=255)                                     aGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCgg                                                                                         >  2:574668/1‑140 (MQ=255)                                     aGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCgg                                                                                         >  2:784695/1‑140 (MQ=255)                                       cgcgTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTa                                                                                       >  1:375784/1‑140 (MQ=255)                                       cgcgTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTa                                                                                       >  2:712209/1‑140 (MQ=255)                                       cgcgTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTACAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTa                                                                                       >  2:540868/1‑140 (MQ=255)                                        gcgTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTg                                                                                            >  1:583043/1‑134 (MQ=255)                                        gcgTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTg                                                                                            <  2:583043/134‑1 (MQ=255)                                        gcgTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTaa                                                                                      >  2:3134/1‑140 (MQ=255)                                        gcgTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTaa                                                                                      >  2:220765/1‑140 (MQ=255)                                         cgTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTAAt                                                                                     <  1:10432/140‑1 (MQ=255)                                          gTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTAATa                                                                                    >  2:125490/1‑140 (MQ=255)                                            gAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTAATAcg                                                                                  <  2:189701/140‑1 (MQ=255)                                             aaCGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTAATAcgc                                                                                 >  1:246580/1‑140 (MQ=255)                                                   aaaaaTGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTAATACGCGGGTAt                                                                           <  1:712209/140‑1 (MQ=255)                                                    aaaaTGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTAATACGCGGGTATc                                                                          <  1:371665/140‑1 (MQ=255)                                                     aaaTGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTAATACGCGGGTATCa                                                                         <  1:281053/140‑1 (MQ=255)                                                        tGTAGCGATGAGTTTCGCCTTCCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTAATACGCGGGTATCAGCg                                                                      <  1:736830/140‑1 (MQ=255)                                                           aGCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTAATACGCGGGTATCAGCGGGt                                                                   <  1:125490/140‑1 (MQ=255)                                                           aGCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTAATACGCGGGTATCAGCGGGt                                                                   <  2:372077/140‑1 (MQ=255)                                                            gCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTAATACGCGGGTATCAGCGGGTa                                                                  >  2:72106/1‑140 (MQ=255)                                                                tGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTAATACGCGGGTATCAGCGGGTAAGtt                                                              <  1:497808/140‑1 (MQ=255)                                                                     ttCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTAATACGCGGGTATCAGCGGGTAAGTTaacaa                                                         <  2:50054/140‑1 (MQ=255)                                                                       cGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTAATACGCGGGGATCAGCGGGGAAGTTAACAACt                                                       >  2:623433/1‑140 (MQ=255)                                                                        gCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTAATACGCGGGTATCAGCGGGTAAGTTAACAACTa                                                      <  1:354124/140‑1 (MQ=255)                                                                         ccTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTAATACGCGGGTATCAGCGGGTAAGTTAACAACTAc                                                     <  2:310447/140‑1 (MQ=255)                                                                          cTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTAATACGCGGGTATCAGCGGGTAAGTTAACAACTAcc                                                    <  2:375784/140‑1 (MQ=255)                                                                            ttCGGCGGTGCTGCGCCATCGGACCCGGGTTTAACAAAGGCGGTACGCGTCTGCAAAACACCGTCTGGCACTGCTACCAGGCCAGAGCCAAACCCTTTCGGTAATACGCGGGTATCACCGGGTGAGATAAAAACCAcccc                                                  >  2:600593/1‑139 (MQ=255)                                                                              cggcggTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTAATACGCGGGTATCAGCGGGTAAGTTAACAACTACCCAGt                                                <  1:72106/140‑1 (MQ=255)                                                                                    tgctgcGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTAATACGCGGGTATCAGCGGGTAAGTTAACAACTACCCAGTGccacc                                          >  1:100413/1‑140 (MQ=255)                                                                                      ctgcGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTAATACGCGGGTATCAGCGGGTAAGTTAACAACTACCCAGTGCCACCag                                        >  1:79008/1‑140 (MQ=255)                                                                                                          ttACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTAATACGCGGGTATCAGCGGGTAAGTTAACAACTACCCAGTGCCACCAGCCGGAGCCGGTTGGCGCATc                    <  2:100413/140‑1 (MQ=255)                                                                                                           tACCAAAGTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTAATACGCGGGTATCAGCGGGTAAGTTAACAACTACCCAGTGCCACCAGCCGGAGCCGGTTGGCGCATcc                   >  2:380148/1‑140 (MQ=255)                                                                                                                  gTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTAATACGCGGGTATCAGCGGGTAAGTTAACAACTACCCAGTGCCACCAGCCGGAGCCGGTTGGCGCATc                    >  1:743896/1‑132 (MQ=255)                                                                                                                  gTCGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTAATACGCGGGTATCAGCGGGTAAGTTAACAACTACCCAGTGCCACCAGCCGGAGCCGGTTGGCGCATc                    <  2:743896/132‑1 (MQ=255)                                                                                                                    cGGTACGCGTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTAATACGCGGGTATCAGCGGGTAAGTTAACAACTACCCAGTGCCACCAGCCGGAGCCGGTTGGCGCATCCGGGTCGTAg          <  1:784695/140‑1 (MQ=255)                                                                                                                         cgcgTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTAATACGCGGGTATCAGCGGGTAAGTTAACAACTACCCAGTGCCACCGGCCGGAGCCGGTTGGCGCATCCGGGTCGTAGCAGGt     <  1:87730/140‑1 (MQ=255)                                                                                                                            gTCTGCAAAACTCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTAATACGCGGGTATCAGCGGGTAAGTTAACAACTACCCAGTGCCACCAGCCGGAGCCGGTTGGCGCATCCGGGTCGTAGCAGGTGac  >  1:243113/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                 CTGGCACCTTCATCGACATCAATACGTTCTATATCCAGCGCGTGAACGGTAAAAATGTAGCGATGAGTTTCGCCTTTCGGCGGTGCTGCGCCATCGTACCCGGTTTTACCAAAGTCGGTACGCGTCTGCAAAACGCCGTCTGGCATTGCTACCAGACCAGAGCCAAACCCTTGCGGTAATACGCGGGTATCAGCGGGTAAGTTAACAACTACCCAGTGCCACCAGCCGGAGCCGGTTGGCGCATCCGGGTCGTAGCAGGTGAC  >  NC_000913/807506‑807768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑