Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,106,335	A→C	L298V (TTG→GTG)	wbbH ←	putative O‑antigen polymerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,106,335	0	A	C	100.0%	304.1 / NA	80	L298V (TTG→GTG)	wbbH	putative O‑antigen polymerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (51/29);  total (51/29)

TCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAACGC  >  NC_000913/2106203‑2106470                                                                                                                                     |                                                                                                                                        tCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAcacccacc                                                                                                                                  <  1:393229/140‑1 (MQ=255)  cacaGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACacc                                                                                                                                      >  2:67572/1‑135 (MQ=255)  cacaGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACacc                                                                                                                                      <  1:67572/135‑1 (MQ=255)      gATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCcacac                                                                                                                             >  2:301332/1‑140 (MQ=255)           gTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAAtt                                                                                                                        <  1:341818/140‑1 (MQ=255)            tAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTc                                                                                                                       >  2:376662/1‑140 (MQ=255)             aaTTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCt                                                                                                                      <  1:69928/140‑1 (MQ=255)                 tCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTAt                                                                                                                  >  2:260878/1‑140 (MQ=255)                 tCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTAt                                                                                                                  >  2:275147/1‑140 (MQ=255)                 tCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTAt                                                                                                                  >  1:749417/1‑140 (MQ=255)                 tCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTAt                                                                                                                  >  1:320856/1‑140 (MQ=255)                 tCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTAt                                                                                                                  >  1:598661/1‑140 (MQ=255)                   gagaCAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGc                                                                                                                >  2:655602/1‑140 (MQ=255)                   gagaCAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGc                                                                                                                >  1:65915/1‑140 (MQ=255)                    agaCAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCTACACAAATTCTTTATGCa                                                                                                               <  2:44071/140‑1 (MQ=255)                    agaCAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCa                                                                                                               <  1:147397/140‑1 (MQ=255)                    agaCAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCa                                                                                                               <  1:33053/140‑1 (MQ=255)                    agaCAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCa                                                                                                               >  2:753918/1‑140 (MQ=255)                    agaCAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCa                                                                                                               >  2:697203/1‑140 (MQ=255)                     gaCAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGcga                                                                                                              <  1:699621/140‑3 (MQ=255)                     gaCAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCaa                                                                                                              <  1:470268/140‑1 (MQ=255)                      aCAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAAc                                                                                                             <  1:613767/140‑1 (MQ=255)                          tctcCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACa                                                                                                         >  1:529866/1‑140 (MQ=255)                                   aaCACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTc                                                                                                <  1:336179/140‑1 (MQ=255)                                   aaCACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTc                                                                                                <  2:529866/140‑1 (MQ=255)                                        cTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCAcc                                                                                            >  1:405760/1‑139 (MQ=255)                                        cTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCAcc                                                                                            <  2:405760/139‑1 (MQ=255)                                        cTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCt                                                                                           >  1:375059/1‑140 (MQ=255)                                        cTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCt                                                                                           >  1:209249/1‑140 (MQ=255)                                        cTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCt                                                                                           >  1:537102/1‑140 (MQ=255)                                           aaaTACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGtt                                                                                        >  2:511285/1‑140 (MQ=255)                                                cAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAg                                                                                   <  1:511285/140‑1 (MQ=255)                                                     aTGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCa                                                                              >  1:533707/1‑140 (MQ=255)                                                     aTGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCa                                                                              >  1:719527/1‑140 (MQ=255)                                                      tGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCAt                                                                             >  2:708503/1‑140 (MQ=255)                                                        aaTAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCa                                                                           >  1:751973/1‑140 (MQ=255)                                                             ccATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATa                                                                                    <  1:2088/126‑1 (MQ=255)                                                             ccATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATa                                                                                    >  2:2088/1‑126 (MQ=255)                                                             ccATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCtt                                                                      >  2:142730/1‑140 (MQ=255)                                                                   tcaAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCaaaa                                                                <  1:142730/140‑1 (MQ=255)                                                                        tagcttagctCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCa                                                           <  1:275147/140‑1 (MQ=255)                                                                         agcttagctCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCaa                                                          <  2:209249/140‑1 (MQ=255)                                                                           cttagctCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCaaaa                                                        >  1:315182/1‑140 (MQ=255)                                                                           cttagctCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCaaaa                                                        <  1:637848/140‑1 (MQ=255)                                                                           cttagctCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCaaaa                                                        >  2:637141/1‑140 (MQ=255)                                                                           cttagctCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCaaaa                                                        >  1:443362/1‑140 (MQ=255)                                                                           cttagctCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCaaaa                                                        >  1:326552/1‑140 (MQ=255)                                                                              agctCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGAc                                                     >  1:34200/1‑140 (MQ=255)                                                                              agctCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGAc                                                     >  1:320782/1‑140 (MQ=255)                                                                              agctCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGAc                                                     >  1:481078/1‑140 (MQ=255)                                                                                ctCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGTAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACAt                                                   >  2:134984/1‑140 (MQ=255)                                                                                   cgcgGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATgag                                                <  1:462838/140‑1 (MQ=255)                                                                                     cgGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGaa                                              >  1:746735/1‑140 (MQ=255)                                                                                     cgGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGaa                                              >  2:127594/1‑140 (MQ=255)                                                                                     cgGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGaa                                              >  1:544031/1‑140 (MQ=255)                                                                                     cgGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGaa                                              >  2:267783/1‑140 (MQ=255)                                                                                         aaTATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTgg                                          <  2:315182/140‑1 (MQ=255)                                                                                         aaTATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTgg                                          <  1:260878/140‑1 (MQ=255)                                                                                           tataAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCa                                        <  2:537102/140‑1 (MQ=255)                                                                                              aaaCATAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCagag                                     <  2:34200/140‑1 (MQ=255)                                                                                                   tAATCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGa                                >  1:607332/1‑140 (MQ=255)                                                                                                    aaTCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGAt                               >  1:226988/1‑140 (MQ=255)                                                                                                    aaTCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGAt                               >  1:447649/1‑140 (MQ=255)                                                                                                    aaTCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGAt                               >  2:446174/1‑140 (MQ=255)                                                                                                     aTCCGAAAAAGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATa                              >  2:254672/1‑140 (MQ=255)                                                                                                     aTCCGAAAAAGCAGTATAAACATTTGTCGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATa                              >  2:282694/1‑140 (MQ=255)                                                                                                           aaaaGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCt                        >  1:756634/1‑140 (MQ=255)                                                                                                              aGCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGaa                     >  2:498273/1‑140 (MQ=255)                                                                                                               gCAGTATAAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGaaa                    >  2:500170/1‑140 (MQ=255)                                                                                                                   tataAACATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACGGGCAGAGTTAGATTCTTGCTGAAAATaa                <  2:769590/140‑1 (MQ=255)                                                                                                                       aaCATTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACt            >  1:240298/1‑140 (MQ=255)                                                                                                                          aTTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCt         >  2:129695/1‑140 (MQ=255)                                                                                                                          aTTTGTTGGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCt         >  1:154268/1‑140 (MQ=255)                                                                                                                            ttgttgGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGa       <  2:746735/140‑1 (MQ=255)                                                                                                                             tgttgGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGaa      >  2:73515/1‑140 (MQ=255)                                                                                                                              gttgGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGaaa     <  2:326552/140‑1 (MQ=255)                                                                                                                               ttgGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAAc    >  2:52416/1‑140 (MQ=255)                                                                                                                               ttgGCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAAc    >  1:226430/1‑140 (MQ=255)                                                                                                                                 ggCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAACCTCCTGAACcgc  <  2:598661/140‑1 (MQ=255)                                                                                                                                 ggCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAAcgc  <  2:452323/140‑1 (MQ=255)                                                                                                                                 ggCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAAcgc  <  2:447649/140‑1 (MQ=255)                                                                                                                                 ggCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAAcgc  <  1:73515/140‑1 (MQ=255)                                                                                                                                 ggCACACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAAcgc  <  2:42797/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        TCACAGATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAACGC  >  NC_000913/2106203‑2106470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑