Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,274,8650TG28.9% 73.6 / 15.3 38T15P (ACC→CCC) garPgalactarate/glucarate/glycerate transporter GarP
Reads supporting (aligned to +/- strand):  ref base T (12/15);  new base G (11/0);  total (23/15)
Fisher's exact test for biased strand distribution p-value = 2.23e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GAAGATGTAACCCATCGAAACCGCACTTAACTGCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCGGGAA  >  NC_000913/3274731‑3275003
                                                                                                                                      |                                                                                                                                          
gaagaTGTAACCCATCGAAACCGCACTTAACTGCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCa                                                                                                                                       <  1:289644/140‑1 (MQ=255)
 aagaTGTAACCCATCGAAACCGCACTTAACTGCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGccc                                                                                                                                      >  1:448053/1‑138 (MQ=255)
             aTCGAAACCGCACTTAACTGCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCttttt                                                                                                                          <  2:165242/140‑1 (MQ=255)
             aTCGAAACCGCACTTAACTGCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCttttt                                                                                                                          <  2:475205/140‑1 (MQ=255)
             aTCGAAACCGCACTTAACTGCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGGATGCACGCCTTTCttttt                                                                                                                          >  1:482622/1‑140 (MQ=255)
              tCGAAACCGCACTTAACTGCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAAAAAAAAAAATCAGTAATATTAAATAGCGGGGATGCACGCCTTTCtttttt                                                                                                                         >  2:7669/1‑140 (MQ=255)
               cGAAACCGCACTTAACTGCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCttttttt                                                                                                                        >  1:357554/1‑140 (MQ=255)
                      gCACTTAACTGCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTC‑TTTTTTCGTCAACg                                                                                                                <  2:248798/140‑1 (MQ=255)
                            aaCTGCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTc                                                                                                           <  1:410763/140‑1 (MQ=255)
                            aaCTGCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGGATGCACGCCCTTCTTTTTTTTGTCAACGGGGTc                                                                                                           >  2:231997/1‑140 (MQ=255)
                                    ctctTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCa                                                                                                   <  2:357554/140‑1 (MQ=255)
                                    ctctTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAAAAATCAGTAATATTAAATAGCGGGGATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCa                                                                                                   >  1:387622/1‑140 (MQ=255)
                                               cTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTAcc                                                                                        <  2:482622/140‑1 (MQ=255)
                                                 tCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGGATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTc                                                                                      >  1:303437/1‑140 (MQ=255)
                                                 tCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGGAACAATAAAAAAAATCAGTAATATTAAATAGCGGGGATGCACGCCTTTCTTTTTTTTTTCAACGGTGGCCAGAATCATTTTTTTTACCTc                                                                                      >  2:376596/1‑140 (MQ=255)
                                                          gCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAAAAATCAGTAATATTAAATAGCGGGGATGCACGCCCTTCTTTTTTTTGTCAACGGTGTTCAGAATCATTTTATTTTCCTCcgggatttt                                                                             >  2:480947/1‑134 (MQ=255)
                                                                     cGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGGATGCACGCCTTTCTTTTTTTCGTCAACGGGGTCCAGAATCATTTTATTTACCTCGGGGACTTTTGCTGtttttt                                                                  >  1:6900/1‑140 (MQ=255)
                                                                     cGTTGCACGATCGGCGTAGTTAACGGCGGTAACAAAAAAAAAAATCAGTAAAATTAAATAGCGGGGATGCACGCCTTTCTTTTTTTTGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGAttttt                                                                  >  2:289442/1‑140 (MQ=255)
                                                                      gTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTtta                                                                 <  2:192904/140‑1 (MQ=255)
                                                                        tGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGAGGGGATGCACGCCTTTTTTTTTTTTGTCAAAGGGGTCCAGAATCATTTTTTTTACCTCGGGGACTTTTGtttttttttttt                                                               >  2:261289/1‑140 (MQ=255)
                                                                        tGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAAAAATCAGTAATATTAAATAGCGGGGATGCACGCCCTTTTTTTTTTTGTCCACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGTTTTttatt                                                               >  2:252982/1‑140 (MQ=255)
                                                                               tCGGCGTAGTTAACGGCGGTAACAATAAAAAAAATCAGGAATATTAAATAGCGGGGATGCACGCCTTTTTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATgg                                                        >  1:159044/1‑140 (MQ=255)
                                                                                  gCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGa                                                     <  1:376596/140‑1 (MQ=255)
                                                                                  gCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGa                                                     <  2:478554/140‑1 (MQ=255)
                                                                                    gTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAg                                                   <  2:517284/140‑1 (MQ=255)
                                                                                    gTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAg                                                   <  2:448053/140‑1 (MQ=255)
                                                                                    gTAGTTAACGGCGGTAACAATAAAAAAAATCAGTAATATTAAATAGCGGGGATGCACGCCTTTCTTTTTTTTGTCAACGGGGTCCAGAATCATTTTTTTTACCTCGGGTACTTTTTCTGGTTTTTTTTATTATGGGGGAg                                                   >  2:314201/1‑140 (MQ=255)
                                                                                            cggcggTAATAATAAAAATAAAAAGGAAAATTTAAAAGGGGGGATGCACGCCTTTCTTTTTTTTGTCAAAGGGGTCCAAAATCATTTTTTTTACCTCGGGGACTTATGGTTGTTTTTATTATTTTTGGGGAGGGGttttt                                           >  2:299958/1‑140 (MQ=255)
                                                                                                ggTAACAATAAAAATAATCAGGAATATTAAATAGCGGGTATGCACGCCCTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTTTTTACCTCGGGGACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATg                                       >  1:143956/1‑140 (MQ=255)
                                                                                                 gTAACAATAAAAATAATCAGTAATATTAAATAGCGGGGATGCACGCCTTTCTTTTTTTTGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGGACTTATGCTGATTTTTATTATTTTGGGGGAGGGGTTATTTATGa                                      >  1:425701/1‑140 (MQ=255)
                                                                                                  tAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAg                                     >  1:448871/1‑140 (MQ=255)
                                                                                                  tAACAATAAAAAAAATCAGTAATATTAAAAAACGGGGATGCACGCCTTTCTTTTTTTTGTCAACCGTGTCCAGAATCATTTTATTTTCCTCCGGTACTTATTTTGATTTTTTTTATTTTTGGGGAGGGGTTATTTTTGAg                                     >  2:143338/1‑140 (MQ=255)
                                                                                                            aataatCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTAt                           >  2:88861/1‑140 (MQ=255)
                                                                                                                atCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCg                       >  1:53814/1‑140 (MQ=255)
                                                                                                                atCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCg                       >  2:263136/1‑140 (MQ=255)
                                                                                                                  cAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTa                     >  2:99669/1‑140 (MQ=255)
                                                                                                                          ttAAATAGCGGGTATGCACGCTTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAAt             <  2:264551/140‑1 (MQ=255)
                                                                                                                               tAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACt        >  2:190948/1‑140 (MQ=255)
                                                                                                                                 gCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCg      <  1:282814/140‑1 (MQ=255)
                                                                                                                                  cGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGTCAATGAACTCgg     >  2:40884/1‑140 (MQ=255)
                                                                                                                                  cGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCgg     >  1:286556/1‑140 (MQ=255)
                                                                                                                                  cGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCgg     >  2:51606/1‑140 (MQ=255)
                                                                                                                                     gTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCGGGaa  <  1:231997/140‑1 (MQ=255)
                                                                                                                                     gTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCGGGaa  <  2:286556/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                          
GAAGATGTAACCCATCGAAACCGCACTTAACTGCAACTCTTTTGCCACTTCGGTACCAGCAATAGACAGCGTTGCACGATCGGCGTAGTTAACGGCGGTAACAATAAAAATAATCAGTAATATTAAATAGCGGGTATGCACGCCTTTCTTTTTTTCGTCAACGGTGTCCAGAATCATTTTATTTACCTCGGGTACTTATGCTGATTTTTATTATTATGGGGAAGGTGTTATTTATGAGTTTCATTTATGCCGTAACGACAATGAACTCGGGAA  >  NC_000913/3274731‑3275003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑