Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,292,111	C→A	L100L (CTG→CTT)	ccmH ←	holocytochrome c synthetase ‑ thiol:disulfide oxidoreductase CcmH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,292,111	0	C	A	100.0%	69.0 / NA	21	L100L (CTG→CTT)	ccmH	holocytochrome c synthetase ‑ thiol:disulfide oxidoreductase CcmH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (10/11);  total (10/11)

GCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTCAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCATACACTTTCTGACGCAGGTCGGT  >  NC_000913/2291977‑2292233                                                                                                                                       |                                                                                                                           gCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCTAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTAAgcgg                                                                                                                       >  1:244092/1‑140 (MQ=255)   aCGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTAAgcggcg                                                                                                                     >  1:199198/1‑140 (MQ=255)       ttACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTAAGCGGCGTTaa                                                                                                                 >  1:366846/1‑140 (MQ=255)                                gAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTAAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTg                                                                                        >  1:52719/1‑140 (MQ=255)                                gAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTAAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTg                                                                                        >  1:377128/1‑140 (MQ=255)                                   aCGCTCCCGGCACCACGCGTCCCCGACGCCGCGGGGGGGCTTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTAAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTGCCg                                                                                     <  1:365014/140‑1 (MQ=255)                                     gCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTAAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTGCCGTa                                                                                   >  1:324811/1‑140 (MQ=255)                                        cccGGCCCCCCGCGTCCCCGCCGCCGCGCGCGGGCGTATATCACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTAAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTGCCGTAAcg                                                                                <  1:520555/139‑1 (MQ=255)                                          cGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTAAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTGCCGTAAcgcg                                                                              <  1:330911/140‑1 (MQ=255)                                           ggCCCCACGGGTCCCCGACGCCGCGAGCGGGCGTATATGCCCAAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTAAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTGCCGTAACgcgc                                                                             <  1:86750/140‑1 (MQ=255)                                           gcccccccgcgTCCCCGCCGCGGCGGGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTAAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTGCCGTAACgcgc                                                                             <  2:466511/133‑1 (MQ=255)                                           gcccccccgcgTCCCCGCCGCCGGGGGGGGGCTTATATGCCCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTAAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTGCCGTAACgcgc                                                                             <  2:155876/133‑1 (MQ=255)                                           gcccccacGCGTACCCGGCCCGGCGGGGGGGCGTATATGACCCACCCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTAAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTGCCGTAACgcgc                                                                             <  2:366846/136‑1 (MQ=255)                                             ccccacGCGTCCCGGACGCCGCGGGCGGGCGTATATGCCCAAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTAAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTGCCGTAACGCGcca                                                                           <  1:257492/138‑1 (MQ=255)                                               ccccgcgTCCCCGCCGCCGCGGGCGGGCGTATATGCCCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTAAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTGCCGTAACGCGccacc                                                                         <  2:283442/137‑1 (MQ=255)                                                                                       aCCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTAAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCa                                 >  2:394602/1‑140 (MQ=255)                                                                                         cGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTAAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATc                               <  2:13475/140‑1 (MQ=255)                                                                                         cGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTAAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATc                               <  2:96779/140‑1 (MQ=255)                                                                                                          actactGGCAGCACCCACAGCAGCACGGTAAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCCTCCTGCATCAGTTCATACACTTTCTg              >  2:63666/1‑140 (MQ=255)                                                                                                                 gcagcaCCCACAGCAGCACGGTAAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCATACACTTTCTGACGCAgg       >  2:330095/1‑140 (MQ=255)                                                                                                                      aCCCACAGCAGCACGGTAAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCTTATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCATACACTTTCTGACGCAGGTCGGt  >  1:196880/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                           GCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTCAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTTACCTTCCTGCATCAGTTCATACACTTTCTGACGCAGGTCGGT  >  NC_000913/2291977‑2292233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑