Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,524,711	G→T	G56G (GGC→GGA)	xapA ←	xanthosine phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,524,711	0	G	T	100.0%	106.7 / NA	31	G56G (GGC→GGA)	xapA	xanthosine phosphorylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (18/13);  total (18/13)

CGAATTGCGTCAGTCATAATGGTCATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAGGTTTATAAGTCTTGATAATATCTATGCA  >  NC_000913/2524574‑2524842                                                                                                                                          |                                                                                                                                    cGAATTGCGTCAGTCATAATGGTCATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATcc                                                                                                                                   >  1:74263/1‑140 (MQ=255)                       tCATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAAtt                                                                                                             >  1:17625/1‑140 (MQ=255)                        cATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTg                                                                                                            >  1:427492/1‑140 (MQ=255)                          ttCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCg                                                                                                          >  1:487284/1‑140 (MQ=255)                             cACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACa                                                                                                       <  1:525100/140‑1 (MQ=255)                              aCGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAg                                                                                                      <  1:195885/140‑1 (MQ=255)                              aCGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGAGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAg                                                                                                      <  1:297773/140‑1 (MQ=255)                               cGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAg                                                                                                      <  2:350520/139‑1 (MQ=255)                               cGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAg                                                                                                      >  1:350520/1‑139 (MQ=255)                                gACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCg                                                                                                    >  1:30865/1‑140 (MQ=255)                                     tCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTtctc                                                                                               >  1:144104/1‑140 (MQ=255)                                     tCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTtctc                                                                                               >  1:374060/1‑140 (MQ=255)                                     tCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTtc                                                                                                 >  1:335791/1‑138 (MQ=255)                                     tCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTtc                                                                                                 <  2:335791/138‑1 (MQ=255)                                         aGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATc                                                                                           <  2:487284/140‑1 (MQ=255)                                                  cgcgACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGcc                                                                                  >  1:361813/1‑140 (MQ=255)                                                  cgcgACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGcc                                                                                  >  1:117000/1‑140 (MQ=255)                                                                 atacCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCg                                                                   <  2:361813/140‑1 (MQ=255)                                                                  tacCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCgg                                                                  <  2:292803/140‑1 (MQ=255)                                                                     caccGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAAc                                                               >  1:68091/1‑140 (MQ=255)                                                                     caccGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAAc                                                               >  2:307359/1‑140 (MQ=255)                                                                      accGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAAcc                                                              <  2:17625/140‑1 (MQ=255)                                                                          gTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTaaa                                                          >  2:101166/1‑140 (MQ=255)                                                                                        tGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCg                                            >  1:421112/1‑140 (MQ=255)                                                                                                   tAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAAt                                 <  2:144104/140‑1 (MQ=255)                                                                                                      cTCCCCCGCATGACCATGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAg                              <  2:451705/140‑1 (MQ=255)                                                                                                                      tGTACCGTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAGGTTTATAAGTCTTGat              <  2:421112/140‑1 (MQ=255)                                                                                                                            gTACTTACCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAGGTTTATAAGTCTTGATAATATc        >  2:247091/1‑140 (MQ=255)                                                                                                                                  aCCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAGGTTTATAAGTCTTGATAATATCTATGCa  >  2:485870/1‑140 (MQ=255)                                                                                                                                  aCCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAGGTTTATAAGTCTTGATAATATCTATGCa  >  1:367912/1‑140 (MQ=255)                                                                                                                                  aCCGGGAATCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAGGTTTATAAGTCTTGATAATATCTATGCa  <  2:74263/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                    CGAATTGCGTCAGTCATAATGGTCATTCCACGACCTTCGTAGAAATGTCCGCGACCTTTCATACATACCACCGGTACGCCCTGGAGATGGCCCAGCACTAACTCCCCCGCATGACCATGTACCGTACTTACCGGGAAGCCCGGCAGCTTTTCGTAGGAAATTGCGACAGCGTTCTCAATCTGATCGGCCAGCGCGCCGAGCCCGGAACCTAAAATAAAGGCCACTCGTGGCGTGAAATCAGGTTTATAAGTCTTGATAATATCTATGCA  >  NC_000913/2524574‑2524842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑