Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,367,561	+G	coding (491/969 nt)	arnC →	undecaprenyl‑phosphate 4‑deoxy‑4‑formamido‑L‑arabinose transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,367,560	1	.	G	100.0%	46.7 / NA	14	R164G (CGC→GGC)	arnC	undecaprenyl‑phosphate 4‑deoxy‑4‑formamido‑L‑arabinose transferase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (10/4);  total (10/4)

TACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGC‑GCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTT  >  NC_000913/2367450‑2367690                                                                                                                |                                                                                                                                   tACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGGCGCCTATCGCCGTCATATTGTcgatgc                                                                                                        >  1:214640/1‑140 (MQ=255) tACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGGCGCCTATCGCCGTCATATTGTcgatgc                                                                                                        >  2:118549/1‑140 (MQ=255)  aCGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGGCGCCTATCGCCGTCATATTGTcgatgcg                                                                                                       >  1:327272/1‑140 (MQ=255)       aGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGGCGCCTATCGCCGTCATATTGTCGATGCGATGtt                                                                                                  <  1:118549/140‑1 (MQ=255)            cGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACt                                                                                             <  1:329579/140‑1 (MQ=255)                    cAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGaa                                                                                     <  2:298993/140‑1 (MQ=255)                       cTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACgc                                                                                  >  1:51618/1‑140 (MQ=255)                                        cTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGAt                                                                 >  2:228139/1‑140 (MQ=255)                                        cTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGAt                                                                 >  2:249465/1‑140 (MQ=255)                                        cTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGAt                                                                 >  2:86598/1‑140 (MQ=255)                                         ttCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGAtt                                                                <  1:249465/140‑1 (MQ=255)                                                                     cgcACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGCGGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCa                                    >  2:106901/1‑140 (MQ=255)                                                                                   aGCGATGGGTGATTACGGTTGTATGCTGCGGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTa                      >  1:304007/1‑140 (MQ=255)                                                                                                       gTATGCTGCGGCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGtt  >  1:327708/1‑140 (MQ=255)                                                                                                                |                                                                                                                                   TACGCCAGAACCGCCAGGACAGCTGGTTTCGTAAAACCGCTTCGAAGATGATTAACCGGCTTATTCAGCGCACCACTGGCAAAGCGATGGGTGATTACGGTTGTATGCTGC‑GCGCCTATCGCCGTCATATTGTCGATGCGATGTTGCACTGCCATGAACGCAGCACCTTTATCCCGATTCTGGCGAATATCTTCGCCCGCCGTGCCATTGAAATTCCAGTACATCATGCCGAGCGTGAGTT  >  NC_000913/2367450‑2367690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑