Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,294,130	G→T	L259M (CTG→ATG)	ccmF ←	holocytochrome c synthetase membrane subunit CcmF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,294,130	0	G	T	100.0%	60.9 / NA	19	L259M (CTG→ATG)	ccmF	holocytochrome c synthetase membrane subunit CcmF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (8/11);  total (8/11)

TGTACCGATACCAGCACGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCAGCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAGCGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCG  >  NC_000913/2293997‑2294242                                                                                                                                      |                                                                                                                 tgtACCGATACCAGCACGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCAGCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCATCGCAGt                                                                                                            <  2:20770/140‑1 (MQ=255)                  gCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCAGCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCATCGCAGTCCCCACCAGCCACGGCa                                                                                           >  1:283696/1‑140 (MQ=255)                   ccGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCAGCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCATCGCAGTCCCCACCAGCCACGGCAt                                                                                          >  1:310717/1‑140 (MQ=255)                          gCACGAGGAAGGTCCCCAACAGACAAAAGGAGAAGGCACTGATTGCCAGCAGTAATGTCCACGCTTTGAAGCTGGCCCGTTGCTCATTGACCGCCAGTGAGTGCATCATCGCAGTCCCCACCAGCCACGGCATAAACGAg                                                                                   <  2:351095/140‑1 (MQ=255)                          gCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCAGCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCATCGCAGTCCCCACCAGCCACGGCATAAACGAg                                                                                   <  2:129699/140‑1 (MQ=255)                                 gAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCAGCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCATCGCAGTCCCCACCAGCCACGGCATAAACGAGGCGtt                                                                              <  1:158980/138‑1 (MQ=255)                                 gAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCAGCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCATCGCAGTCCCCACCAGCCACGGCATAAACGAGGCGtt                                                                              >  2:158980/1‑138 (MQ=255)                                       ccccACCAGACACAACGAGAAGGCACTGATTGCCAGCAGTAATGTCCACGCTTGGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCATCGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCg                                                                      <  2:34019/140‑1 (MQ=255)                                                         gaAGGCACTGATTGCCAGCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCATCGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGc                                                    <  2:346769/140‑1 (MQ=255)                                                            ggCACTGATTGCCAGCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCATCGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCAc                                                 >  1:186116/1‑140 (MQ=255)                                                                  gATTGCCGGCAGTAATGCCCCCCCTTTGAAGCTGCGGCGTTTTTCGGTGACCGCCAGTGAGTGCATCATCGCAGTCCCCACCAGCCACGGCATAACCGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGc                                           <  2:364959/140‑1 (MQ=37)                                                                       ccAGCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCATCGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGt                                      <  2:115691/140‑1 (MQ=255)                                                                                  gTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCATCGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTCGTAATAGGc                           <  1:186045/140‑1 (MQ=255)                                                                                        gCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCATCGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCGCCCCAGCCGAGTTCGTAATAGGCCCATGc                     >  2:34850/1‑140 (MQ=255)                                                                                        gCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCATCGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTCGTAATAGGCCCATGc                     >  1:82828/1‑140 (MQ=255)                                                                                                    gcgcGTTGTTCAGTGACCGCCAGTGAGTGCATCATCGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCAc         >  2:238350/1‑140 (MQ=255)                                                                                                           gttCAGTGACCGCCAGTGAGTGCATCATCGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCg  <  2:186116/140‑1 (MQ=255)                                                                                                                   aCCGCCAGTGAGTGCATCATCGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGcc   <  1:37992/131‑1 (MQ=255)                                                                                                                   aCCGCCAGTGAGTGCATCATCGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGcc   >  2:37992/1‑131 (MQ=255)                                                                                                                                      |                                                                                                                 TGTACCGATACCAGCACGCCGGAACGCACGAGGAAAGTCCCCAACAGACACAACGAGAAGGCACTGATTGCCAGCAGTAATGTCCACGCTTTGAAGCTGGCGCGTTGTTCAGTGACCGCCAGTGAGTGCATCAGCGCAGTCCCCACCAGCCACGGCATAAACGAGGCGTTTTCTACCGGATCCCAGAACCACCAGCCACCCCAGCCGAGTTCGTAATAGGCCCATGCGGAACCGAGCACGATGCCG  >  NC_000913/2293997‑2294242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑