Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,651,105	C→A	D395Y (GAT→TAT)	yfhM ←	alpha2‑macroglobulin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,651,105	0	C	A	100.0%	14.9 / NA	6	D395Y (GAT→TAT)	yfhM	alpha2‑macroglobulin
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (4/2);  total (4/2)

GCCCATCGGGTTTAATCACGTCTAACTTGATGGGTTGATTGGGCAACGCTTTACCGTCTGCATCACGCAGCAAACCATTGAGGATTACCGTTTCACCTGGGCGATAAAGATCGCGTGGGCCAAACATGAAAAACTGTTTGCTATAGCCTGGCGCGCCAGCAATGTTAAATTCTGCTAAGTCCAGCGCCGGAAGTTTTAAATCGAGTAGCGTTGTCTGACCGTCTTTACGCGCCAACAG  >  NC_000913/2650994‑2651231                                                                                                                |                                                                                                                               gCCCATCGGGTTTAATCACGTCTAACTTGATGGGTTGATTGGGCAACGCTTTACCGTCTGCATCACGCAGCAAACCATTGAGGATTACCGTTTCACCTGGGCGATAAAGATAGCGTGGGCCAAACATGAAAAACTGTTTg                                                                                                    >  2:152859/1‑140 (MQ=255)                      cTAACTTGATGGGTTGATTGGGCAACGCTTTACCGTCTGCATCACGCAGCAAACCATTGAGGATTACCGTTTCACCTGGGCGATAAAGATAGCGTGGGCCAAACATGAAAAACTGTTTGCTATAGCCTGGCGCGCCAGCa                                                                               <  2:315835/140‑1 (MQ=255)                              aTGGGTTGATTGGGCAACGCTTTACCGTCTGCATCACGCAGCAAACCATTGAGGATTACCGTTTCACCTGGGCGATAAAGATAGCGTGGGCCAAACATGAAAAACTGTTTGCTATAGCCTGGCGCGCCAGCAATGTTaaa                                                                       >  1:28864/1‑140 (MQ=255)                                   ttGATTGGGCAACGCTTTACCGTCTGCATCACGCAGCAAACCATTGAGGATTACCGTTTCACCTGGGCGTTAAAGATAGCGTGGGCCAAACATGAAAAACTGTTTGCTATAGCCTGGCGCGCCAGCAATGTTAAATTCTg                                                                  >  1:163387/1‑140 (MQ=255)                                                                 aCGCAGCAAACCATTGAGGATTACCGTTTCACCTGGGCGATAAAGATAGCGTGGGCCAAACATGAAAAACTGTTTGCTATAGCCTGGCGCGCCAGCAATGTTAAATTCTGCTAAGTCCAGCGCCGGAAGTTTTAAATCGa                                    <  1:220008/140‑1 (MQ=255)                                                                                                   gggCGATAAAGATAGCGTGGGCCAAACATGAAAAACTGTTTGCTATAGCCTGGCGCGCCAGCAATGTTAAATTCTGCTAAGTCCAGCGCCGGAAGTTTTAAATCGAGTAGCGTTGTCTGACCGTCTTTACGCGCCAACAg  >  2:261995/1‑140 (MQ=255)                                                                                                                |                                                                                                                               GCCCATCGGGTTTAATCACGTCTAACTTGATGGGTTGATTGGGCAACGCTTTACCGTCTGCATCACGCAGCAAACCATTGAGGATTACCGTTTCACCTGGGCGATAAAGATCGCGTGGGCCAAACATGAAAAACTGTTTGCTATAGCCTGGCGCGCCAGCAATGTTAAATTCTGCTAAGTCCAGCGCCGGAAGTTTTAAATCGAGTAGCGTTGTCTGACCGTCTTTACGCGCCAACAG  >  NC_000913/2650994‑2651231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑