Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,771,942	G→T	S71S (TCC→TCA)	yfjU ←	CP4‑57 prophage; ArsC family protein YfjU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,771,942	0	G	T	100.0%	78.5 / NA	23	S71S (TCC→TCA)	yfjU	CP4‑57 prophage; ArsC family protein YfjU
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (12/11);  total (12/11)

TTGGTCTAAGCGATGGTTTCATATGATCAACCATTCTTGATAAACCCATGAGACGGACTAACTTTTTCATTAAAATTAAAAAGATATGAATGAATACTGCGGATATCAACATGGCCAATCACATTCAAGGACAAGGCAGGCATCAGGTGACATTGCTCCCTGATGCACTCGATGATTTTGTCACTGAAGATATCTGAAATAAGTTTAATCAGTTCATCACGGGTCGGTGGCATATCGAGATAATTAATTATTGTCGG  >  NC_000913/2771814‑2772070                                                                                                                                 |                                                                                                                                 ttGGTCTAAGCGATGGTTTCATATGATCAACCATTCTTGATAAACCCATGAGACGGACTAACTTTTTCATTAAAATTAAAAAGATATGAATGAATACTGCGGATATCAACATGGCCAATCACATTCAATGACAaggcagg                                                                                                                       <  2:81582/140‑1 (MQ=255)        aaGCGATGGTTTCATATGATCAACCATTCTTGATAAACCCATGAGACGGACTAACTTTTTCATTAAAATTAAAAAGATATGAATGAACACTGCGGATATCAGCATGGCCAATCACATTCAATGACAAGGCAGGCATCAgg                                                                                                                <  2:7349/140‑1 (MQ=255)               ggTTTCATATGATCAACCATTCTTGATAAACCCATGAGACGGACTAACTTTTTCATTAAAATTAAAAAGATATGAATGAATACTGCGGATATCAACATGGCCAATCACATTCAATGACAAGGCAGGCATCAGGTGACAtt                                                                                                         >  2:113340/1‑140 (MQ=255)                gTTTCATATGATCAACCATTCTTGATAAACCCATGAGACGGACTAACTTTTTCATTAAAATTAAAAAGATATGAATGAATACTGCGGATATCAACATGGCCAATCACATTCAATGACAAGGCAGGCATCAGGTGACATTg                                                                                                        <  1:269793/140‑1 (MQ=255)                          aTCAACCATTCTTGATAAACCCATGAGACGGACTAACTTTTTCATTAAAATTAAAAAGATATGAATGAATACTGCGGATATCAACATGGCCAATCACATTCAATGACAAGGCAGGCATCAGGTGACATTGCTCCCTGATg                                                                                              >  2:196058/1‑140 (MQ=255)                          aTCAACCATTCTTGATAAACCCATGAGACGGACTAACTTTTTCATTAAAATTAAAAAGATATGAATGAATACTGCGGATATCAACATGGCCAATCACATTCAATGACAAGGCAGGCATCAGGTGACATTGCTCCCTGATg                                                                                              >  1:222887/1‑140 (MQ=255)                           tCAACCATTCTTGATAAACCCATGAGACGGACTAACTTTTTCATTAAAATTAAAAAGATATGAATGAATACTGCGGATATCAACATGGCCAATCACATTCAATGACAAGGCAGGCATCAGGTGACATTGCTCCCTGATGc                                                                                             >  1:70319/1‑140 (MQ=255)                               ccATTCTTGATAAACCCATGAGACGGACTAACTTTTTCATTAAAATTAAAAAGATATGAATGAATACTGCGGATATCAACATGGCCAATCACATTCAATGACAAGGCAGGCATCAGGTGACATTGCTCCCTGATGCACTc                                                                                         >  1:87037/1‑140 (MQ=255)                                  ttCTTGATAAACCCATGAGACGGACTAACTTTTTCATTAAAATTAAAAAGATATGAATGAATACTGCGGATATCAACATGGCCAATCACATTCAATGACAAGGCAGGCATCAGGTGACATTGCTCCCTGATGCACTCgat                                                                                      >  1:168950/1‑140 (MQ=255)                                            aCCCATGAGACGGACTAACTTTTTCATTAAAATTAAAAAGATATGAATGAATACTGCGGATATCAACATGGCCAATCACATTCAATGACAAGGCAGGCATCAGGTGACATTGCTCCCTGATGCACTCGATGATTTTGTCa                                                                            <  1:228860/140‑1 (MQ=255)                                                       ggACTAACTTTTTCATTAAAATTAAAAAGATATGAATGAATACTGCGGATATCAACATGGCCAATCACATTCAATGACAAGGCAGGCATCAGGTGACATTGCTCCCTGATGCACTCGATGATTTTGTCACTGAAGATATc                                                                 <  1:231403/140‑1 (MQ=255)                                                       ggACTAACTTTTTCATTAAAATTAAAAAGATATGAATGAATACTGCGGATATCAACATGGCCAATCACATTCAATGACAAGGCAGGCATCAGGTGACATTGCTCCCTGATGCACTCGATGATTTTGTCACTGAAGATATc                                                                 >  2:138359/1‑140 (MQ=255)                                                                                     tatGAATGAATACTGCGGATATCAACATGGCCAATCACATTCAATGACAAGGCAGGCATCAGGTGACATTGCTCCCTGATGCACTCGATGATTTTGTCACTGAAGATATCTGAAATAAGTTTAATCAGTTCATCACGGGt                                   <  1:317779/140‑1 (MQ=255)                                                                                     tatGAATGAATACTGCGGATATCAACATGGCCAATCACATTCAATGACAAGGCAGGCATCAGGTGACATTGCTCCCTGATGCACTCGATGATTTTGTCACTGAAGATATCTGAAATAAGTTTAATCAGTTCATCACGGGt                                   <  1:138359/140‑1 (MQ=255)                                                                                       tgaatgaaTACTGCGGATATCAACATGGCCAATCACATTCAATGACAAGGCAGGCATCAGGTGACATTGCTCCCTGATGCACTCGATGATTTTGTCACTGAAGATATCTGAAATAAGTTTAATCAGTTCATCACGGGTCg                                 <  1:154070/140‑1 (MQ=255)                                                                                         aatgaatACTGCGGATATCAACATGGCCAATCACATTCAATGACAAGGCAGGCATCAGGTGACATTGCTCCCTGATGCACTCGATGATTTTGTCACTGAAGATATCTGAAATAAGTTTAATCAGTTCATCACGGGTCGGt                               <  1:113340/140‑1 (MQ=255)                                                                                                   gCGGATATCAACATGGCCAATCACATTCAATGACAAGGCAGGCATCAGGTGACATTGCTCCCTGATGCACTCGATGATTTTGTCACTGAAGATATCTGAAATAAGTTTAATCAGTTCATCACGGGTCGGTGGCATATCga                     >  1:94630/1‑140 (MQ=255)                                                                                                   gCGGATATCAACATGGCCAATCACATTCAATGACAAGGCAGGCATCAGGTGACATTGCTCCCTGATGCACTCGATGATTTTGTCACTGAAGATATCTGAAATAAGTTTAATCAGTTCATCACGGGTCGGTGGCATATCga                     >  1:245034/1‑140 (MQ=255)                                                                                                    cGGATATCAACATGGCCAATCACATTCAATGACAAGGCAGGCATCAGGTGACATTGCTCCCTGATGCACTCGATGATTTTGTCACTGAAGATATCTGAAATAAGTTTAATCAGTTCATCACGGGTCGGTGGCATATCgag                    >  2:46703/1‑140 (MQ=255)                                                                                                       atatCAACATGGCCAATCACATTCAATGACAAGGCAGGCATCAGGTGACATTGCTCCCTGATGCACTCGATGATTTTGTCACTGAAGATATCTGAAATAAGTTTAATCAGTTCATCACGGGTCGGTGGCATATCGAGAta                 >  1:150703/1‑140 (MQ=255)                                                                                                          tCAACATGGCCAATCACATTCAATGACAAGGCAGGCATCAGGTGACATTGCTCCCTGATGCACTCGATGATTTTGTCACTGAAGATATCTGAAATAAGTTTAATCAGTTCATCACGGGTCGGTGGCATATCGAGAtaatt              <  2:70319/140‑1 (MQ=255)                                                                                                                   ccAATCACATTCAATGACAAGGCAGGCATCAGGTGACATTGCTCCCTGATGCACTCGATGATTTTGTCACTGAAGATATCTGAAATAAGTTTAATCAGTTCATCACGGGTCGGTGGCATATCGAGATAATTAATTATTGt     >  1:24586/1‑140 (MQ=255)                                                                                                                      aTCACATTCAATGACAAGGCAGGCATCAGGTGACATTGCTCCCTGATGCACTCGATGATTTTGTCACTGAAGATATCTGAAATAAGTTTAATCAGTTCATCACGGGTCGGTGGCATATCGAGATAATTAATTATTGTCgg  <  2:168950/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                 TTGGTCTAAGCGATGGTTTCATATGATCAACCATTCTTGATAAACCCATGAGACGGACTAACTTTTTCATTAAAATTAAAAAGATATGAATGAATACTGCGGATATCAACATGGCCAATCACATTCAAGGACAAGGCAGGCATCAGGTGACATTGCTCCCTGATGCACTCGATGATTTTGTCACTGAAGATATCTGAAATAAGTTTAATCAGTTCATCACGGGTCGGTGGCATATCGAGATAATTAATTATTGTCGG  >  NC_000913/2771814‑2772070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑