Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,940,353	C→T	W297* (TGG→TGA)	rlmM ←	23S rRNA 2'‑O‑ribose C2498 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,940,353	0	C	T	100.0%	53.0 / NA	17	W297* (TGG→TGA)	rlmM	23S rRNA 2'‑O‑ribose C2498 methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/10);  total (7/10)

CCGCGCCTGAATCTGAGCATTTATGCCATGTTCATCAAGCTGTGCCTGAATATACGCCAGATTGTGTGACACTTCTTCGTAGCGTTTTTTCATCGGCAGTTTGAGGTTGAAAATGGTTTCACGGCACCAGCCATTAACCAGCCACTGCGCCATCAATGCCGCAACTTTCGCCGGTTTTTCAACCATATCGCATACCATCCAGGAGATATTGCTGCGCGTCGGACGGAATTTGAAACCGTCTTCCCGCAGCCACGTCAC  >  NC_000913/2940227‑2940484                                                                                                                               |                                                                                                                                    ccGCGCCTGAATCTGAGCATTTATGCCATGTTCATCAAGCTGAGCCTGAATATACGCCAGATTGTGTGACACTTCTTCGTAGCGTTTTTTCATCGGCAGTTTGAGGTTGAAAATGGTTTCACGGCATCAGCCATTAAACa                                                                                                                        >  2:51710/1‑140 (MQ=255)   gcgcCTGAATCTGAGCATTTATGCCATGTTCATCAAGCTGTGCCTGAATATACGCCAGATTGTGTGACACTTCTTCGTAGCGTTTTTTCATCGGCAGTTTGAGGTTGAAAATGGTTTCACGGCATCAGCCATTAACCAGc                                                                                                                      >  1:36353/1‑140 (MQ=255)   gcgcCTGAATCTGAGCATTTATGCCATGTTCATCAAGCTGTGCCTGAATATACGCCAGATTGTGTGACACTTCTTCGTAGCGTTTTTTCATCGGCAGTTTGAGGTTGAAAATGGTTTCACGGCATCAGCCATTAACCAGc                                                                                                                      >  2:13095/1‑140 (MQ=255)    cgcCTGAATCTGAGCATTTATGCCATGTTCATCAAGCTGTGCCTGAATATACGCCAGATTGTGTGACACTTCTTCGTAGCGTTTTTTCATCGGCAGTTTGAGGTTGAAAATGGTTTCACGGCATCAGCCATTAACCAGcc                                                                                                                     >  2:27461/1‑140 (MQ=255)         gAATCTGAGCATTTATGCCATGTTCATCAAGCTGTGCCTGAATATACGCCAGATTGTGTGACACTTCTTCGTAGCGTTTTTTCATCGGCAGTTTGAGGTTGAAAATGGTTTCACGGCATCAGCCATTAACCAGCCACTgc                                                                                                                <  2:13304/140‑1 (MQ=255)                    tttATGCCATGTTCATCAAGCTGTGCCTGAATATACGCCAGATTGTGTGACACTTCTTCGTAGCGTTTTTTCATCGGCAGTTTGAGGTTGAAAATGGTTTCACGGCATCAGCCATTAACCAGCCACTGCGCCATCAATGc                                                                                                     >  2:261319/1‑140 (MQ=255)                                 catcaAGCTGTGCCTGAATATACGCCAGATTGTGTGACACTTCTTCGTAGCGTTTTTTCATCGGCAGTTTGAGGTTGAAAATGGTTTCACGGCATCAGCCATTAACCAGCCACTGCGCCATCAATGCCGCAACTTTCGcc                                                                                        <  2:294791/140‑1 (MQ=255)                                           ggCCTGAATATACGCCAGATTGTGTGACACTTCTTCGTAGCGTTTTTTCATCGGCAGTTTGAGGTTGAAAATGGTTTCACGGCATCAGCCATTAACCAGCCACTGCGCCATCAATGCCGCAACTTTCGCCGGTTTTTCaa                                                                              >  2:108424/2‑140 (MQ=255)                                              cTGAATATACGCCAGATTGTGTGACACTTCTTCGTAGCGTTTTTTCATCGGCAGTTTGAGGTTGAAAATGGTTTCACGGCATCAGCCATTAACCAGCCACTGCGCCATCAATGCCGCAACTTTCGCCGGTTTTTCAACCa                                                                           <  1:56624/140‑1 (MQ=255)                                                     taCGCCAGATTGTGTGACACTTCTTCGTAGCGTTTTTTCATCGGCAGTTTGAGGTTGAAAATGGTTTCACGGCATCAGCCATTAACCAGCCACTGCGCCATCAATGCCGCAACTTTCGCCGGTTTTTCAACCATATCGCa                                                                    >  1:155776/1‑140 (MQ=255)                                                     taCGCCAGATTGTGTGACACTTCTTCGTAGCGTTTTTTCATCGGCAGTTTGAGGGTGAAAATGGATTCACGGGATCCGCCATTAACCAGCCACTGCGCCCTCAATGCCGCAACTTTCGCCGGTTTTTCAACCATATCGCa                                                                    >  2:250420/1‑140 (MQ=255)                                                       cGCCAGATTGTGTGACACTTCTTCGTAGCGTTTTTTCATCGGCAGTTTGAGGTTGAAAATGGTTTCACGGCATCAGCCATTAACCAGCCACTGCGCCATCAATGCCGCAACTTTCGCCGGTTTTTCAACCATATCGCATa                                                                  <  1:261319/140‑1 (MQ=255)                                                         ccAGATTGTGTGACACTTCTTCGTAGCGTTTTTTCATCGGCAGTTTGAGGTTGAAAATGGTTTCACGGCATCAGCCATTAACCAGCCACTGCGCCATCAATGCCGCAACTTTCGCCGGTTTTTCAACCATATCGCATAcc                                                                <  1:27461/140‑1 (MQ=255)                                                           aGATTGTGTGACACTTCTTCGTAGCGTTTTTTCATCGGCAGTTTGAGGTTGAAAATGGTTTCACGGCATCAGCCATTAACCAGCCACTGCGCCATCAATGCCGCAACTTTCGCCGGTTTTTCAACCATATCGCATACCAt                                                              <  2:344526/140‑1 (MQ=255)                                                                gtgtgACACTTCTTCGTAGCGTTTTTTCATCGGCAGTTTGAGGTTGAAAATGGTTTCACGGCATCAGCCATTAACCAGCCACTGCGCCATCAATGCCGCAACTTTCGCCGGTTTTTCAACCATATCGCATACCATCCAgg                                                         <  2:343819/140‑1 (MQ=255)                                                                                    gTTTTTTCATCGGCAGTTTGAGGTTGAAAATGGTTTCACGGCATCAGCCATTAACCAGCCACTGCGCCATCAATGCCGCAACTTTCGCCGGTTTTTCAACCATATCGCATACCATCCAGGAGATATTGCTGCGCGTcgga                                     <  2:36353/140‑1 (MQ=255)                                                                                                         ggTTGAAAATGGTTTCACGGCATCAGCCATTAACCAGCCACTGCGCCATCAATGCCGCAACTTTCGCCGGTTTTTCAACCATATCGCATACCATCCAGGAGATATTGCTGCGCGTCGGACGGAATTTGAAACCGTCTTcc                <  2:332870/140‑1 (MQ=255)                                                                                                              aaaaTGGTTTCACGGCATCAGCCATTAACCAGCCACTGCGCCATCAATGCCGCAACTTTCGCCGGTTTTTCAACCATATCGCATACCATCCAGGAGATATTGCTGCGCGTCGGACGGAATTTGAAACCGTCTTCCCGCAg           >  1:164720/1‑140 (MQ=255)                                                                                                                       tCACGGCATCAGCCATTAACCAGCCACTGCGCCATCAATGCCGCAACTTTCGCCGGTTTTTCAACCATATCGCATACCATCCAGGAGATATTGCTGCGCGTCGGACGGAATTTGAAACCGTCTTCCCGCAGCCACGTCAc  <  2:2642/140‑1 (MQ=255)                                                                                                                               |                                                                                                                                    CCGCGCCTGAATCTGAGCATTTATGCCATGTTCATCAAGCTGTGCCTGAATATACGCCAGATTGTGTGACACTTCTTCGTAGCGTTTTTTCATCGGCAGTTTGAGGTTGAAAATGGTTTCACGGCACCAGCCATTAACCAGCCACTGCGCCATCAATGCCGCAACTTTCGCCGGTTTTTCAACCATATCGCATACCATCCAGGAGATATTGCTGCGCGTCGGACGGAATTTGAAACCGTCTTCCCGCAGCCACGTCAC  >  NC_000913/2940227‑2940484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑