Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,089,446	C→A	T388N (ACC→AAC)	galP →	galactose:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,089,446	0	C	A	100.0%	78.7 / NA	24	T388N (ACC→AAC)	galP	galactose:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (8/16);  total (8/16)

ACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGCTGGGTAACGCCAACACCTTCTGGGTGTATGCGGCTCTGAACGTACTGTTTATCCTGC  >  NC_000913/3089311‑3089574                                                                                                                                        |                                                                                                                                 aCTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCAACTGc                                                                                                                              >  1:96136/1‑140 (MQ=255)    ctcCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCAACTGCTcc                                                                                                                           >  1:233881/1‑140 (MQ=255)          cGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCAACTGCTCCACTGcc                                                                                                                     <  2:251824/140‑1 (MQ=255)               cAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCAACTGCTCCACTGCCACCaa                                                                                                                <  2:127936/140‑1 (MQ=255)                     ttCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCAACTGCTCCACTGCCACCAACTGGAt                                                                                                          <  1:118070/140‑1 (MQ=255)                       cgccatcgccatGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCAACTGCTCCACTGCCACCAACTGGATTg                                                                                                        >  2:172456/1‑140 (MQ=255)                             cgccatGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCAACTGCTCCACTGCCACCAACTGGATTGCCAACa                                                                                                  >  1:213943/1‑140 (MQ=255)                                                       gTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCAACTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGAcc                                                                        <  1:195910/140‑1 (MQ=255)                                                                                   ttGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCAACTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGCTGGGTAACGCCAaca                                            <  2:255300/140‑1 (MQ=255)                                                                                   ttGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCAACTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGCTGGGTAACGCCAaca                                            <  1:234025/140‑1 (MQ=255)                                                                                             tgCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCAACTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGCTGGGTAACGCCAACACCTTCTgggg                                  >  1:35524/1‑139 (MQ=255)                                                                                             tgCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCAACTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGCTGGGTAACGCCAACACCTTCTGGgt                                  >  2:115792/1‑140 (MQ=255)                                                                                               cTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCAACTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGCTGGGTAACGCCAACACCTTCTGGgtgt                                >  2:200131/1‑140 (MQ=255)                                                                                                tCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCAACTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGCTGGGTAACGCCAACACCTTCTGGGTGTa                               >  1:175799/1‑140 (MQ=255)                                                                                                    aaaTTCAGCCGCTGAAAGGCCGCGATTTTGGCATCAACTGCTCCACTGCCACCAGCTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGCTGGGTAACGCCAACACCTTCTGGGTGTATGCg                           <  2:233881/140‑1 (MQ=255)                                                                                                           gCCGCTGAAAGGCCGCGATTTTGGCATCAACTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGCTGGGTAACGCCAACACCTTCTGGGTGTATGCGGCTCTGa                    <  1:172456/140‑1 (MQ=255)                                                                                                              gCTGAAAGGCCGCGATTTTGGCATCAACTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGCTGGGTAACGCCAACACCTTCTGGGTGTATGCGGCTCTGAACg                 <  2:45679/140‑1 (MQ=255)                                                                                                              gCTGAAAGGCCGCGATTTTGGCATCAACTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGCTGGGTAACGCCAACACCTTCTGGGTGTATGCGGCTCTGAACg                 <  2:213943/140‑1 (MQ=255)                                                                                                               cTGAAAGGCCGCGATTTTGGCATCAACTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGCTGGGTAACGCCAACACCTTCTGGGTGTATGCGGCTCTGAACGt                <  2:147452/140‑1 (MQ=255)                                                                                                               cTGAAAGGCCGCGATTTTGGCATCAACTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGCTGGGTAACGCCAACACCTTCTGGGTGTATGCGGCTCTGAACGt                <  2:219910/140‑1 (MQ=255)                                                                                                                tGAAAGGCCGCGATTTTGGCATCAACTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGCTGGGTAACGCCAACACCTTCTGGGTGTATGCGGCTCTGAACGTa               <  2:126320/140‑1 (MQ=255)                                                                                                                       ccGCGATTTTGGCATCAACTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGCTGGGTAACGCCAACACCTTCTGGGTGTATGCGGCTCTGAACGTACTGTTTa        <  2:96136/140‑1 (MQ=255)                                                                                                                           gATTTTGGCATCAACTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGCTGGGTAACGCCAACACCTTCTGGGTGTATGCGGCTCTGAACGTACTGTTTATCct    <  1:252726/140‑1 (MQ=255)                                                                                                                             ttttGGCATCAACTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGCTGGGTAACGCCAACACCTTCTGGGTGTATGCGGCTCTGAACGTACTGTTTATCctgc  <  1:281090/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                 ACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGCTGGGTAACGCCAACACCTTCTGGGTGTATGCGGCTCTGAACGTACTGTTTATCCTGC  >  NC_000913/3089311‑3089574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑