Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,766,621	G→T	L95F (TTG→TTT)	yibA →	putative lyase containing HEAT‑repeat

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,766,621	0	G	T	100.0%	85.9 / NA	26	L95F (TTG→TTT)	yibA	putative lyase containing HEAT‑repeat
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (14/12);  total (14/12)

GAGGTGGGCAAGACGCTGTTAGATTGGCAATTGAGTTCTGCTCTGATAAAAATTATATCCGTAGAGATATCGGAGCATTTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTACTGCTTTTG  >  NC_000913/3766485‑3766751                                                                                                                                         |                                                                                                                                   gagGTGGGCAAGACGCTGTTAGATTGGCAATTGAGTTCTGCTCTGATAAAAATTATATCCGTAGAGATATCGGAGCATTTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAAc                                                                                                                                 >  1:207479/1‑140 (MQ=255)      gggCAAGACGCTGTTAGATTGGCAATTGAGTTCTGCTCTGATAAAAATTATATCCGTAGAGATATCGGAGCATTTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAatat                                                                                                                            >  2:329133/1‑140 (MQ=255)       ggCAAGACGCTGTTAGATTGGCAATTGAGTTCTGCTCTGATAAAAATTATATCCGTAGAGATATCGGAGCATTTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAat                                                                                                                              <  2:96832/137‑1 (MQ=255)       ggCAAGACGCTGTTAGATTGGCAATTGAGTTCTGCTCTGATAAAAATTATATCCGTAGAGATATCGGAGCATTTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAat                                                                                                                              >  1:96832/1‑137 (MQ=255)       ggCAAGACGCTGTTAGATTGGCAATTGAGTTCTGCTCTGATAAAAATTATATCCGTAGAGATATCGGAGCATTTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAATATg                                                                                                                           >  1:40537/1‑140 (MQ=255)          aaGACGCTGTTAGATTGGCAATTGAGTTCTGCTCTGATAAAAATTATATCCGTAGAGATATCGGAGCATTTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAATATGGCa                                                                                                                        <  2:75728/140‑1 (MQ=255)           aGACGCTGTTAGATTGGCAATTGAGTTCTGCTCTGATAAAAATTATATCCGTAGAGATATCGGAGCATTTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAATATGGCAt                                                                                                                       <  2:165022/140‑1 (MQ=255)            gACGCTGTTAGATTGGCAATTGAGTTCTGCTCTGATAAAAATTATATCCGTAGAGATATCGGAGCATTTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAATATGGCAtt                                                                                                                      >  2:55526/1‑140 (MQ=255)             aCGCTGTTAGATTGGCAATTGAGTTCTGCTCTGATAAAAATTATATCCGTAGAGATATCGGAGCATTTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAATATGGCATTg                                                                                                                     <  2:306219/140‑1 (MQ=255)                             aaTTGAGTTCTGCTCTGATAAAAATTATATCCGTAGAGATATCGGAGCATTTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAATATGGCATTGAATGATAAGAGCGCtt                                                                                                     >  2:322409/1‑140 (MQ=255)                                  aGTTCTGCTCTGATAAAAATTATATCCGTAGAGATATCGGAGCATTTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAATATGGCATTGAATGATAAGAGCGCTTGCGtt                                                                                                <  2:69665/140‑1 (MQ=255)                                      cTGCTCTGATAAAAATTATATCCGTAGAGATATCGGAGCATTTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAg                                                                                            >  1:104243/1‑140 (MQ=255)                                              aTAAAAATTATATCCGTAGAGATATCGGAGCATTTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCa                                                                                    <  1:92988/140‑1 (MQ=37)                                                 aaaaTTATATCCGTAGAGATATCGGAGCATTTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATc                                                                                 <  2:6472/140‑1 (MQ=25)                                                     ttATATCCGTAGAGATATCGGAGCATTTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGt                                                                             >  2:205355/1‑140 (MQ=25)                                                        tatCCGTAGAGATATCGGAGCATTTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCaa                                                                          >  1:299883/1‑140 (MQ=25)                                                        tatCCGTAGAGATATCGGAGCATTTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCaa                                                                          <  2:104243/140‑1 (MQ=25)                                                                     atCGGAGCATTTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATg                                                             >  2:239537/1‑140 (MQ=255)                                                                                    cTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAAt                                              <  1:337490/140‑1 (MQ=255)                                                                                      cGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAAttt                                            >  2:187825/1‑140 (MQ=255)                                                                                       gggCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTa                                           >  1:308104/1‑140 (MQ=255)                                                                                         gCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTAtt                                         >  2:4216/1‑140 (MQ=255)                                                                                               aaaaaTTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTa                                   <  2:49391/140‑1 (MQ=255)                                                                                                aaaaTTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTaa                                  <  2:255768/140‑1 (MQ=255)                                                                                                aaaaTTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTTAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTaa                                  <  2:135673/140‑1 (MQ=255)                                                                                                                                tAATATTTTTAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTACTGCTTTTg  >  2:264842/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                   GAGGTGGGCAAGACGCTGTTAGATTGGCAATTGAGTTCTGCTCTGATAAAAATTATATCCGTAGAGATATCGGAGCATTTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTACTGCTTTTG  >  NC_000913/3766485‑3766751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑