Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,194,562	C→A	G513V (GGG→GTG)	thiC ←	phosphomethylpyrimidine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,194,562	0	C	A	100.0%	51.1 / NA	17	G513V (GGG→GTG)	thiC	phosphomethylpyrimidine synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (8/9);  total (8/9)

TCGAGGGCCAGATTAAACTGGTCTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACGTGAAGTGGTCATAGCCCGGCGCAATA  >  NC_000913/4194427‑4194696                                                                                                                                        |                                                                                                                                       tCGAGGGCCAGATTAAACTGGTCTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTGCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGcacccg                                                                                                                                    >  2:257558/1‑138 (MQ=255)                        ttCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGCACCTGCTTAACATCTTCTTTATTGGGCa                                                                                                             <  1:45600/140‑1 (MQ=255)                                    ttCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGCACCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGct                                                                                                 >  2:143935/1‑140 (MQ=255)                                           cGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGCACCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTgg                                                                                          <  2:157906/140‑1 (MQ=255)                                                      cATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGCACCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAAc                                                                               <  1:6614/140‑1 (MQ=255)                                                                                  ggATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGCACCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAat                                                   <  1:143935/140‑1 (MQ=255)                                                                                            tCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGCACCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCa                                         <  1:66734/140‑1 (MQ=255)                                                                                                             tGGGCAGCAATCTTATAGGTGATAAGCACCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCAc                                        <  2:31128/124‑1 (MQ=255)                                                                                                             tGGGCAGCAATCTTATAGGTGATAAGCACCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCAc                                        >  1:31128/1‑124 (MQ=255)                                                                                                             tGGGCAGCAATCTTATAGGTGATAAGCACCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACGTGaa                        <  1:233068/140‑1 (MQ=255)                                                                                                                                 gATAAGCACCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACGTGAAGTGGTCATAGCCCGGCGCaa    >  2:7439/1‑140 (MQ=255)                                                                                                                                 gATAAGCACCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACGTGAAGTGGTCATAGCCCGGCGCaa    <  2:136541/140‑1 (MQ=255)                                                                                                                                  aTAAGCACCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACGTGAAGTGGTCATAGCCCgg         <  2:257373/134‑1 (MQ=255)                                                                                                                                  aTAAGCACCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACGTGAAGTGGTCATAGCCCgg         >  1:257373/1‑134 (MQ=255)                                                                                                                                  aTAAGCACCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACGTGAAGTGGTCATAGCCCGGCGCAat   >  1:295243/1‑140 (MQ=255)                                                                                                                                  aTAAGCACCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACGTGAAGTGGTCATAGCCCGGCGCAat   >  2:279320/1‑140 (MQ=255)                                                                                                                                  aTAAGCACCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACGTGAAGTGGTCATAGCCCGGCGCAat   >  1:120838/1‑140 (MQ=255)                                                                                                                                   tAAGCACCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACGTGAAGTGGTCATAGCCCGGCGCAata  >  1:128332/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       TCGAGGGCCAGATTAAACTGGTCTTCCCAGCGAAATTCGAAGCGGGCTTTCGACATGGCGTTATCGCGAATTTGCGCGCCCGGATGCCCTTTCGCCAGGTCAGCGGCGTGGGCAGCAATCTTATAGGTGATAAGCCCCTGCTTAACATCTTCTTTATTGGGCAGACCCAGATGCTCTTTTGGCGTTACGTAACAGAGCATCGCGCAGCCAAACCAGCCAATCATCGCCGCACCAATCCCCGACGTGAAGTGGTCATAGCCCGGCGCAATA  >  NC_000913/4194427‑4194696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑