Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,061,902	C→A	A35E (GCG→GAG)	torD →	trimethylamine‑N‑oxide reductase‑specific chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,061,902	0	C	A	100.0%	35.5 / NA	12	A35E (GCG→GAG)	torD	trimethylamine‑N‑oxide reductase‑specific chaperone
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (11/1);  total (11/1)

GCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGC  >  NC_000913/1061768‑1062007                                                                                                                                       |                                                                                                          gCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGAGCAGa                                                                                                      <  1:91757/140‑1 (MQ=255) gCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGAGCAGa                                                                                                      >  2:93405/1‑140 (MQ=255)            cccGCGTCGCAGGTGAAATCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACGGACGCAAATCGCCAGTGAGCAGATGGCTGAATgg                                                                                           >  1:363237/1‑140 (MQ=255)               gcgTCGCAGGTGAAATCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGAGCAGATGGCTGAATGGttt                                                                                        >  1:109125/1‑140 (MQ=255)                                   aCCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGAGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAAcc                                                                    >  1:152209/1‑140 (MQ=255)                                          tGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGAGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAACCGCCGCTc                                                             >  2:87980/1‑140 (MQ=255)                                                cacaACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGAGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAACCGCCGCTCACTgcg                                                       >  2:87800/1‑140 (MQ=255)                                                            ccTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGAGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAACCGCCGCTCACTGCGGCGGTGAACGAg                                           >  1:247727/1‑140 (MQ=255)                                                               gtgtTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGAGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAACCGCCGCTCACTGCGGCGGTGAACGAGCTg                                        >  2:107510/1‑140 (MQ=255)                                                                tgtTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGAGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAACCGCCGCTCACTGCGGCGGTGAACGAGCTgg                                       >  2:350081/1‑140 (MQ=255)                                                                                      ttgttCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGAGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGAc                 >  2:306733/1‑140 (MQ=255)                                                                                              ccGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGAGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCCGATTGCCAAGCTGACAGGACGTg         >  1:86137/1‑140 (MQ=255)                                                                                                     cTGGACGATGAACAACTGACGCAAATCGCCAGTGAGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGc  <  2:152209/140‑1 (MQ=255)                                                                                                                                       |                                                                                                          GCGAATATGTTCCCGCGTCGCAGGTGAAATCATGACCACGCTGACAGCACAACAGATTGCCTGTGTTTACGCCTGGCTAGCGCAGTTGTTCTCCCGTGAGCTGGACGATGAACAACTGACGCAAATCGCCAGTGCGCAGATGGCTGAATGGTTTTCGTTGCTGAAAAGCGAACCGCCGCTCACTGCGGCGGTGAACGAGCTGGAAAACCGTATTGCCACGCTGACAGTACGTGACGATGC  >  NC_000913/1061768‑1062007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑