Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,291,179	G→T	S313I (AGC→ATC)	rssB →	regulator of RpoS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,291,179	0	G	T	100.0%	70.7 / NA	21	S313I (AGC→ATC)	rssB	regulator of RpoS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (10/11);  total (10/11)

TATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCGGTGGTCGACTGCGCTTGATGTTGTCTGCAGAATGAGCAAACGATAACGCGGGCTAAATTTGCATTACCTGCTAATGTCGGCTGG  >  NC_000913/1291043‑1291304                                                                                                                                         |                                                                                                                              tatCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGATCCa                                                                                                                            >  2:53971/1‑140 (MQ=255) tatCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGATCCa                                                                                                                            >  1:354286/1‑140 (MQ=255)      tcGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGATCCAgcgat                                                                                                                       >  2:373420/1‑140 (MQ=255)                  aaaTCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGATCCAGCGATGCGATGCCTGGc                                                                                                           <  1:303356/140‑1 (MQ=255)                  aaaTCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGATCCAGCGATGCGATGCCTGGc                                                                                                           <  1:32119/140‑1 (MQ=255)                                                     cGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGATCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCGGTGGTCGACTgcgc                                                                        <  2:359679/140‑1 (MQ=255)                                                        tAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGATCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCGGTGGTCGACTGCGCTTg                                                                     >  1:372112/1‑140 (MQ=255)                                                            tACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGATCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCGGTGGTCGACTGCGCTTGAtgt                                                                 <  2:372112/140‑1 (MQ=255)                                                               cGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGATCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCGGTGGTCGACTGCGCTTGAtgttgt                                                              >  1:68690/1‑140 (MQ=255)                                                               cGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGATCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCGGTGGTCGACTGCGCTTGAtgttgt                                                              >  1:297334/1‑140 (MQ=255)                                                                                     aGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGATCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCGGTGGTCGACTGCGCTTGATGTTGTCTGCAGAATGAGCAAACGATaa                                        >  2:232791/1‑140 (MQ=255)                                                                                           ggTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGATCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCGGTGGTCGACTGCGCTTGATGTTGTCTGCAGAATGAGCAAACGATAACGCggg                                  <  1:171677/140‑1 (MQ=255)                                                                                               ttCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGATCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCGGTGGTCGACTGCGCTTGATGTTGTCTGCAGAATGAGCAAACGATAACGCGGGCTaa                              >  2:133027/1‑140 (MQ=255)                                                                                                      aGGCACTTTAGGTAACGCTTATTTGAATCAATTGATCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCGGTGGTCGACTGCGCTTGATGTTGTCTGCAGAATGAGCAAACGATAACGCGGGCTAAATTTGCa                       >  2:103560/1‑140 (MQ=255)                                                                                                       ggCACTTTAGGTAACGCTTATTTGAATCAATTGATCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCGGTGGTCGACTGCGCTTGATGTTGTCTGCAGAATGAGCAAACGATAACGCGGGCTAAATTTGCAt                      <  1:135910/140‑1 (MQ=255)                                                                                                       ggCACTTTAGGTAACGCTTATTTGAATCAATTGATCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCGGTGGTCGACTGCGCTTGATGTTGTCTGCAGAATGAGCAAACGATAACGCGGGCTAAATTTGCAt                      >  1:260581/1‑140 (MQ=255)                                                                                                                       cTTATTTGAATCAATTGATCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCGGTGGTCGACTGCGCTTGATGTTGTCTGCAGAATGAGCAAACGATAACGCGGGCTAAATTTGCATTACCTGCTAATGTCgg      <  1:345520/140‑1 (MQ=255)                                                                                                                         tATTTGAATCAATTGATCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCGGTGGTCGACTGCGCTTGATGTTGTCTGCAGAATGAGCAAACGATAACGCGGGCTAAATTTGCATTACCTGCTAATGTCGGCt    <  1:28616/140‑1 (MQ=255)                                                                                                                          aTTTGAATCAATTGATCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCGGTGGTCGACTGCGCTTGATGTTGTCTGCAGAATGAGCAAACGATAACGCGGGCTAAATTTGCATTACCTGCTAATGTCGGCtg   <  2:40700/140‑1 (MQ=255)                                                                                                                           tttGAATCAATTGATCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCGGTGGTCGACTGCGCTTGATGTTGTCTGCAGAATGAGCAAACGATAACGCGGGCTAAATTTGCATTACCTGCTAATGTCGGCtgg  <  2:139851/140‑1 (MQ=255)                                                                                                                           tttGAATCAATTGATCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCGGTGGTCGACTGCGCTTGATGTTGTCTGCAGAATGAGCAAACGATAACGCGGGCTAAATTTGCATTACCTGCTAATGTCGGCtgg  <  2:218782/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                              TATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGACGTTAAATACCGGCGAACACCAGGTGCAAATCAGTAATGGTGTTCCGTTAGGCACTTTAGGTAACGCTTATTTGAATCAATTGAGCCAGCGATGCGATGCCTGGCAATGCCAAATATGGGGAACCGGTGGTCGACTGCGCTTGATGTTGTCTGCAGAATGAGCAAACGATAACGCGGGCTAAATTTGCATTACCTGCTAATGTCGGCTGG  >  NC_000913/1291043‑1291304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑