Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,368,220	G→T	A48S (GCG→TCG)	pspA →	phage shock protein A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,368,220	0	G	T	100.0%	71.7 / NA	23	A48S (GCG→TCG)	pspA	phage shock protein A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (14/9);  total (14/9)

TTTCTCGCTTTGCCGACATCGTGAATGCCAACATCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCGTATTGAACAAGCGTCGGCGCGTGAGGTTGAATGGCAGGAAAAAGCCGAACTGGCGCTGCTGAAAGAGAGAGAGGATCTGGCACGTGCAGCGTTAAT  >  NC_000913/1368089‑1368353                                                                                                                                    |                                                                                                                                      tttCTCGCTTTGCCGACATCGTGAATGCCAACTTCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGTcgcgcgcg                                                                                                                               <  2:219451/140‑1 (MQ=255)                              aaCATCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGTCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCg                                                                                                  >  1:132107/1‑140 (MQ=255)                                                     aGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGTCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCGTATTGAACAAGCGTCGGCGCGTg                                                                           >  1:200649/1‑140 (MQ=255)                                                       cGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGTCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCGTATTGAACAAGCGTCg                                                                                  >  1:182499/1‑131 (MQ=255)                                                       cGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGTCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCGTATTGAACAAGCGTCg                                                                                  <  2:78287/131‑1 (MQ=255)                                                       cGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGTCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCGTATTGAACAAGCGTCg                                                                                  <  2:182499/131‑1 (MQ=255)                                                       cGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGTCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCGTATTGAACAAGCGTCg                                                                                  >  1:78287/1‑131 (MQ=255)                                                       cGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGTCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCGTATTGAACAAGCGTCGGCGCGTGAg                                                                         >  1:300430/1‑140 (MQ=255)                                                       cGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGTCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCGTATTGAACAAGCGTCGGCGCGTGAg                                                                         >  1:235533/1‑140 (MQ=255)                                                                        cTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGTCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCGTATTGAACAAGCGTCGGCGCGTGAGGTTGAATGGCAGGaaaa                                                        >  2:252848/1‑140 (MQ=255)                                                                         tGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGTCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCGTATTGAACAAGCGTCGGCGCGTGAGGTTGAATGGCAGGaaaaa                                                       >  1:319444/1‑140 (MQ=255)                                                                                tCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGTCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCGTATTGAACAAGCGTCGGCGCGTGAGGTTGAATGGCAGGAAAAAGTCGAAc                                                >  1:31968/1‑140 (MQ=255)                                                                                        tCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGTCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCGTATTGAACAAGCGTCGGCGCGTGAGGTTGAATGGCAGGAAAAAGCCGAACTGGCgctg                                        <  1:41692/140‑1 (MQ=255)                                                                                        tCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGTCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCGTATTGAACAAGCGTCGGCGCGTGAGGTTGAATGGCAGGAAAAAGCCGAACTGGCgctg                                        <  2:667/140‑1 (MQ=255)                                                                                               gaTGGAAGATACACTGGTTGAAGTACGTTCTACTTCGTCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCGTATTGAACAAGCGTCGGCGCGTGAGGTTGAATGGCAGGAAAAAGCCGAACTGGCGCTGCTGAAag                                 <  1:241204/140‑1 (MQ=255)                                                                                                 tGGAAGATACACTGGTTGAAGTACGTTCTACTTCGTCGCGCGCGTTAGCAGAAAAGAAACAGCTGACTCGCCGTATTGAACAAGCGTCGGCGCGTGAGGTTGAATGGCAGGAAAAAGCCGAACTGGCGCTGCTGAAagag                               >  1:291579/1‑140 (MQ=255)                                                                                                     aGATACACTGGTTGAAGTACGTTCTACTTCGTCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCGTATTGAACAAGCGTCGGCGCGTGAGGTTGAATGGCAGGAAAAAGCCGAACTGGCGCTGCTGAAagagagag                           <  2:235533/140‑1 (MQ=255)                                                                                                         acacTGGTTGAAGTACGTTCTACTTCGTCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCGTATTGAACAAGCGTCGGCGCGTGAGGTTGAATGGCAGGAAAAAGCCGAACTGGCGCTGCTGAAAGAGAGAGAGGa                       >  2:42875/1‑140 (MQ=255)                                                                                                          cacTGGTTGAAGTACGTTCTACTTCGTCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCGTATTGAACAAGCGTCGGCGCGTGAGGTTGAATGGCAGGAAAAAGCCGAACTGGCGCTGCTGAAAGAGAGAGAGGAt                      <  2:132107/140‑1 (MQ=255)                                                                                                           acTGGTTGAAGTACGTTCTACTTCGTCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCGTATTGAACAAGCGTCGGCGCGTGAGGTTGAATGGCAGGAAAAAGCCGAACTGGCGCTGCTGAAAGAGAGAGAGGATc                     >  1:28573/1‑140 (MQ=255)                                                                                                                  gAAGTACGTTCTACTTCGTCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCGTATTGAACAAGCGTCGGCGCGTGAGGTTGAATGGCAGGAAAAAGCCGAACTGGCGCTGCTGAAAGAGAGAGAGGATCTGGCACg              >  1:182909/1‑140 (MQ=255)                                                                                                                    aGTACGTTCTACTTCGTCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCGTATTGAACAAGCGTCGGCGCGTGAGGTTGAATGGCAGGAAAAAGCCGAACTGGCGCTGCTGAAAGAGAGAGAGGATCTGGCACGTg            <  1:349729/140‑1 (MQ=255)                                                                                                                              aCTTCGTCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCGTATTGAACAAGCGTCGGCGCGTGAGGTTGAATGGCAGGAAAAAGCCGAACTGGCGCTGCTGAAAGAGAGAGAGGATCTGGCACGTGCAGCGTTAAt  >  2:271893/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                      TTTCTCGCTTTGCCGACATCGTGAATGCCAACATCAACGCTCTGTTAGAGAAAGCGGAAGATCCACAGAAACTGGTTCGTCTGATGATCCAGGAGATGGAAGATACACTGGTTGAAGTACGTTCTACTTCGGCGCGCGCGTTGGCAGAAAAGAAACAGCTGACTCGCCGTATTGAACAAGCGTCGGCGCGTGAGGTTGAATGGCAGGAAAAAGCCGAACTGGCGCTGCTGAAAGAGAGAGAGGATCTGGCACGTGCAGCGTTAAT  >  NC_000913/1368089‑1368353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑