Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	650,809	0	T	G	21.4%	134.9 / 12.1	56	T350P (ACC→CCC)	citC	citrate lyase synthetase
Reads supporting (aligned to +/- strand):  ref base T (16/28);  new base G (12/0);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 1.09e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCGGATAT  >  NC_000913/650673‑650947                                                                                                                                         |                                                                                                                                           aaCGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCtt                                                                                                                                         >  1:1222275/1‑140 (MQ=255)    gctgctATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTg                                                                                                                                      <  1:827476/140‑1 (MQ=255)     ctgctATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTTACCTGTTTCTCATGCGGGGGTCTTTTGa                                                                                                                                     >  1:668061/1‑140 (MQ=255)     ctgctATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGa                                                                                                                                     >  1:1226829/1‑140 (MQ=255)                ttGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCcgc                                                                                                                          >  2:186326/1‑140 (MQ=255)                  gCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGAGGGTCTTTTGACGAGCTGCCGCGt                                                                                                                        <  1:779996/140‑1 (MQ=255)                   cAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTc                                                                                                                       >  2:1563090/1‑140 (MQ=255)                   cAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTc                                                                                                                       >  2:17430/1‑140 (MQ=255)                     ggTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCAAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCt                                                                                                                     >  2:1183695/1‑140 (MQ=255)                          atatCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCgg                                                                                                                <  2:1222275/140‑1 (MQ=255)                           tatCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCggg                                                                                                               <  1:1563090/140‑1 (MQ=255)                            atCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGa                                                                                                              <  2:668061/140‑1 (MQ=255)                             tCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAg                                                                                                             <  2:1226829/140‑1 (MQ=255)                                    tATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCaa                                                                                                      >  2:577529/1‑140 (MQ=255)                                     aTCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTTACGAGCTGCCGCGTCCTGGCGGGGGGGGTCaaa                                                                                                     >  2:1159901/1‑139 (MQ=255)                                      cagAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGc                                                                                                    <  1:789901/138‑1 (MQ=255)                                       cGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGa                                                                                                              >  1:1650405/1‑129 (MQ=255)                                       cGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGa                                                                                                              <  2:1650405/129‑1 (MQ=255)                                        gAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAg                                                                                                  <  2:452421/140‑1 (MQ=255)                                         aGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTTACCTGTTTTTCATGCGGGGGGCTTTTTACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAGCAgg                                                                                                 >  1:716678/1‑140 (MQ=255)                                             gggCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCt                                                                                             <  1:1578684/140‑1 (MQ=255)                                                    gCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTTATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCCCAAGCAGGGTCTGCAaatg                                                                                      >  2:789901/1‑139 (MQ=255)                                                        atcatcACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTTACGAGCTGCCGCGTCCTGGCGGGGGGGGTCCAACAGGTTCTTCCAATAAAGc                                                                                  >  1:1241975/1‑140 (MQ=255)                                                         tcatcaCATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCa                                                                                 <  2:1198393/140‑1 (MQ=255)                                                               caTCCCCAGACTCAAGGGTGCCTGCAACGGCGGGGTGGGTTATTTTCTTTTTTTACCTGTTTCTCATGCGGGGGGCTTTTTACGAGCTGCCGCGTCCTGGCGGGGGGGCTCCAACAGGGTTTGCCAATAATGCAGccggg                                                                           >  2:830703/1‑136 (MQ=255)                                                               caTCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGa                                                                           <  1:17430/140‑1 (MQ=255)                                                               caTCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGa                                                                           >  1:1297251/1‑140 (MQ=255)                                                                   cccAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGc                                                                       <  1:1160083/140‑1 (MQ=255)                                                                    ccAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCa                                                                      <  2:1581234/140‑1 (MQ=255)                                                                            aaGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAg                                                              >  1:597000/1‑140 (MQ=255)                                                                            aaGGGTGCCTGCAACGGCGGGCTGGGTTATTTTCATTTTTTACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGTGCTCAAGCAGGGTTTGCAAAAAATGCAGCGGGGCTGCAGGGGCCAg                                                              >  2:1160083/1‑140 (MQ=255)                                                                                 tGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAACAGGGTCTGCAAAAAAATCAGCGGGGCTGCAGGGGCCAgcgggg                                                         >  2:719753/1‑138 (MQ=255)                                                                                   ccTGCAACGGCGGGCTGGTTTATTTTCATTTTTCCCCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGcgcg                                                       <  2:1636845/140‑1 (MQ=255)                                                                                   ccTGCAACGGCGGGCTGGGTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCCAACAGGGTTTGCAAATAAATCAGCGTGGCCGCAGGGACCAGCGGcgcg                                                       >  2:1361670/1‑140 (MQ=255)                                                                                      gCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATa                                                    <  1:830703/140‑1 (MQ=255)                                                                                            gcggGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTg                                              <  1:1620344/140‑1 (MQ=255)                                                                                                           ttCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGcc                               <  1:1159901/140‑1 (MQ=255)                                                                                                             cATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCcag                             <  1:1006378/140‑1 (MQ=255)                                                                                                             cATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCcag                             <  1:186326/140‑1 (MQ=255)                                                                                                              aTTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCcagc                            >  2:1575881/1‑140 (MQ=255)                                                                                                              aTTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCcagc                            >  1:538717/1‑140 (MQ=255)                                                                                                               tttttCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCcag                             >  2:453134/1‑138 (MQ=255)                                                                                                               tttttCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCcag                             <  1:453134/138‑1 (MQ=255)                                                                                                                  ttCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGtt                        <  2:831525/140‑1 (MQ=255)                                                                                                                      ccTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACg                    <  1:719753/140‑1 (MQ=255)                                                                                                                         gTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCATGAGATCGTTTTTCGCCAGCAGTTGACGTAc                 <  2:50163/140‑1 (MQ=255)                                                                                                                            tctcATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCg              >  1:638707/1‑140 (MQ=255)                                                                                                                              tcATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCggg            <  1:13605/140‑1 (MQ=255)                                                                                                                                 tGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAg         >  1:346466/1‑140 (MQ=255)                                                                                                                                 tGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAg         >  2:1397530/1‑140 (MQ=255)                                                                                                                                 tGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAg         <  1:1183695/140‑1 (MQ=255)                                                                                                                                  gCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGc        >  1:1391813/1‑140 (MQ=255)                                                                                                                                    gggggTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCgg      >  1:1212853/1‑140 (MQ=255)                                                                                                                                     ggggTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCGGa     >  1:238013/1‑140 (MQ=255)                                                                                                                                      gggTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCGGat    <  2:1297251/140‑1 (MQ=255)                                                                                                                                       ggTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCGGata   <  2:716678/140‑1 (MQ=255)                                                                                                                                        gTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCGGatat  >  2:1383306/1‑140 (MQ=255)                                                                                                                                        gTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCGGatat  >  1:1324520/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                           AACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCAGGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCGGATAT  >  NC_000913/650673‑650947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑