Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	129,529	C→A	V578F (GTT→TTT)	yacH ←	DUF3300 domain‑containing protein YacH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	129,529	0	C	A	100.0%	346.5 / NA	98	V578F (GTT→TTT)	yacH	DUF3300 domain‑containing protein YacH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (51/47);  total (51/47)

TACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCATACTGCTGACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTAAACACCTGGCGCTGCTCTGGTGATGCGGA  >  NC_000913/129395‑129664                                                                                                                                       |                                                                                                                                        tACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCATACTGCTGACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTtct                                                                                                                                    <  1:1333427/140‑1 (MQ=255)        gCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCATACTGCTGACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGaaa                                                                                                                             >  2:1065750/1‑140 (MQ=255)               aCCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCATACTGCTGACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGa                                                                                                                      <  1:70042/140‑1 (MQ=255)                ccTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCATACTGCTGACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGAc                                                                                                                     >  2:40122/1‑140 (MQ=255)                 cTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCATACTGCTGACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACg                                                                                                                    <  1:40122/140‑1 (MQ=255)                 cTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCATACTGCTGACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACg                                                                                                                    <  1:1204794/140‑1 (MQ=255)                 cTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCATACTGCTGACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACg                                                                                                                    <  2:1624931/140‑1 (MQ=255)                 cTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCATACTGCTGACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACg                                                                                                                    <  2:919235/140‑1 (MQ=255)                           ttcttGCTCCACGTTCAGAGAGACGTTCGCATACTGCTGACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGt                                                                                                          >  2:1512189/1‑140 (MQ=255)                             cttGCTCCACGTTCAGAGAGACGTTCGCATACTGCTGACCGTTGGTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGa                                                                                                        >  2:1439536/1‑140 (MQ=255)                             cttGCTCCACGTTCAGAGAGACGTTCGCATACTGCTGACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACgttgtt                                                                                                               >  1:839249/1‑133 (MQ=255)                             cttGCTCCACGTTCAGAGAGACGTTCGCATACTGCTGACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACgttgtt                                                                                                               <  2:839249/133‑1 (MQ=255)                             cttGCTCCACGTTCAGAGAGACGTTCGCATACTGCTGACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACg                                                                                                                    >  2:1371131/1‑128 (MQ=255)                             cttGCTCCACGTTCAGAGAGACGTTCGCATACTGCTGACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACg                                                                                                                    <  1:1371131/128‑1 (MQ=255)                             cttGCTCCACGTTCAGAGAGACGTTCGCATACTGCTGACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGa                                                                                                        >  1:738310/1‑140 (MQ=255)                              ttGCTCCACGTTCAGAGAGACGTTCGCATACTGCTGACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGAt                                                                                                       <  2:1007897/140‑1 (MQ=255)                              ttGCTCCACGTTCAGAGAGACGTTCGCATACTGCTGACCGTTGCTCGTTATTCAGCCTGACAGTATGGATACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGa                                                                                                                      >  1:546079/1‑125 (MQ=255)                              ttGCTCCACGTTCAGAGAGACGTTCGCATACTGCTGACCGTTGCTCGTTATTCAGCCTGACAGTATGGATACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGa                                                                                                                      <  2:546079/125‑1 (MQ=255)                                      cGTTCAGAGAGACGTTCGCATACTGCTGACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGAt                                                                                                       >  2:1062689/1‑132 (MQ=255)                                      cGTTCAGAGAGACGTTCGCATACTGCTGACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGAt                                                                                                       <  1:1062689/132‑1 (MQ=255)                                      cGTTCAGAGAGACGTTCGCATACTGCTGACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTg                                                                                               >  2:1415897/1‑140 (MQ=255)                                      cGTTCAGAGAGACGTTCGCATACTGCTGACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTg                                                                                               >  2:877080/1‑140 (MQ=255)                                        ttCAGAGAGACGTTCGCATACTGCTGACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGaa                                                                                             <  1:412233/140‑1 (MQ=255)                                        ttCAGAGAGACGTTCGCATACTGCTGACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGaa                                                                                             <  2:471879/140‑1 (MQ=255)                                           agagagACGTTCGCATACTGCTGACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAacac                                                                                          >  2:571892/1‑140 (MQ=255)                                             agagaCGTTCGCATACTGCTGACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCAGACGTTGTTCAGGTGATGCCGATTGAACACGc                                                                                        >  2:1175550/1‑140 (MQ=255)                                                  cGTTCGCATACTGCTGACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTggcg                                                                                   >  2:94210/1‑140 (MQ=255)                                                            ctgctgACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCa                                                                         <  1:94210/140‑1 (MQ=255)                                                              gctgACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACg                                                                       <  2:42322/140‑1 (MQ=255)                                                              gctgACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACg                                                                       <  2:1409639/140‑1 (MQ=255)                                                               ctgACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCGGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGt                                                                      >  1:1622796/1‑140 (MQ=255)                                                               ctgACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTggcgg                                                                                  <  1:700467/128‑1 (MQ=255)                                                               ctgACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTggcgg                                                                                  >  2:700467/1‑128 (MQ=255)                                                               ctgACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTa                                                                            <  1:1196261/134‑1 (MQ=255)                                                               ctgACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTa                                                                            >  2:1196261/1‑134 (MQ=255)                                                               ctgACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGt                                                                      >  1:1450902/1‑140 (MQ=255)                                                               ctgACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGt                                                                      >  2:641841/1‑140 (MQ=255)                                                                tgACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGtt                                                                     <  1:641841/140‑1 (MQ=255)                                                                tgACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGtt                                                                     <  1:1415897/140‑1 (MQ=255)                                                                  aCCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGc                                                                   <  1:877080/140‑1 (MQ=255)                                                                  aCCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGc                                                                   <  1:1635786/140‑1 (MQ=255)                                                                      ttGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGtt                                                               >  2:209073/1‑140 (MQ=255)                                                                       tGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACg                                                                       >  2:595870/1‑131 (MQ=255)                                                                       tGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACg                                                                       <  1:595870/131‑1 (MQ=255)                                                                       tGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGt                                                                      >  1:856587/1‑132 (MQ=255)                                                                       tGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGt                                                                      <  2:982217/132‑1 (MQ=255)                                                                       tGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGt                                                                      >  1:982217/1‑132 (MQ=255)                                                                       tGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGt                                                                      <  2:856587/132‑1 (MQ=255)                                                                       tGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTg                                                              <  1:1512189/140‑1 (MQ=255)                                                                                 tCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTggg                                                    >  2:1434858/1‑140 (MQ=255)                                                                                   aGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGgcg                                                  >  2:1536569/1‑140 (MQ=255)                                                                                    gCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGgcgc                                                 >  1:1306699/1‑140 (MQ=255)                                                                                      cTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCgctg                                               >  2:173190/1‑140 (MQ=255)                                                                                          cAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGt                                           >  2:107480/1‑140 (MQ=255)                                                                                          cAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGt                                           >  1:106159/1‑140 (MQ=255)                                                                                               tGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACttt                                      >  1:1436170/1‑140 (MQ=255)                                                                                               tGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACttt                                      >  2:957666/1‑140 (MQ=255)                                                                                               tGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACttt                                      <  1:603718/140‑1 (MQ=255)                                                                                               tGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACttt                                      <  1:1065750/140‑1 (MQ=255)                                                                                                ggTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACtt                                       >  2:943258/1‑138 (MQ=255)                                                                                                ggTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACtt                                       <  1:943258/138‑1 (MQ=255)                                                                                                   tACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATTACGTTGCTGTTTGTTCAGTGGGCGCTGCTGTACTTTTTcc                                  >  2:888458/1‑140 (MQ=255)                                                                                                   tACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTcc                                  >  2:1036407/1‑140 (MQ=255)                                                                                                    aCTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCt                                 >  1:1029746/1‑140 (MQ=255)                                                                                                    aCTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCt                                 >  1:357508/1‑140 (MQ=255)                                                                                                    aCTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCt                                 >  1:1251743/1‑140 (MQ=255)                                                                                                    aCTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCt                                 >  1:1064272/1‑140 (MQ=255)                                                                                                       gTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGGTTCAGTGTGCGCTCCTGTACTTTTTCCTTaa                              >  2:229661/1‑140 (MQ=255)                                                                                                       gTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTaa                              >  2:506366/1‑140 (MQ=255)                                                                                                       gTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTaa                              >  2:1276714/1‑140 (MQ=255)                                                                                                       gTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTaa                              <  2:106159/140‑1 (MQ=255)                                                                                                       gTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTaa                              >  2:1017610/1‑140 (MQ=255)                                                                                                       gTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTaa                              >  1:24205/1‑140 (MQ=255)                                                                                                       gTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTaa                              >  2:89785/1‑140 (MQ=255)                                                                                                       gTCGTTTAGACGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTaa                              >  2:332387/1‑140 (MQ=255)                                                                                                                 cGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTAAACACCTGgcg                    <  1:1036407/140‑1 (MQ=255)                                                                                                                 cGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTAAACACCTGgcg                    <  1:107480/140‑1 (MQ=255)                                                                                                                 cGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTAAACACCTGgcg                    <  2:1436170/140‑1 (MQ=255)                                                                                                                 cGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTAAACACCTGgcg                    <  2:1306699/140‑1 (MQ=255)                                                                                                                 cGTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTAAACACCTGgcg                    <  1:209073/140‑1 (MQ=255)                                                                                                                  gTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTAAACACCTGgcgc                   <  1:173190/140‑1 (MQ=255)                                                                                                                  gTTGTGGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTAAACACCTGgcgc                   <  1:695245/140‑1 (MQ=255)                                                                                                                    tgtgGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTAAACACCTGGCgctg                 >  2:1419380/1‑140 (MQ=255)                                                                                                                     gtgGGCGGCTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTAAACACCTGGCgctgc                <  1:693672/140‑1 (MQ=255)                                                                                                                         gcggcTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCGGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTAAACACCTGGCGCTGCTCTg            <  2:1622796/140‑1 (MQ=255)                                                                                                                         gcggcTCTCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTAAACACCTGGCGCTGCTCTg            <  2:1058824/140‑1 (MQ=255)                                                                                                                             ctctCCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTAAACACCTGGCGCTGCTCTGGTGa        >  1:1566321/1‑140 (MQ=255)                                                                                                                               ctcCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTAAACACCTGGCGCTGCTCTGGTGATg      <  1:89785/140‑1 (MQ=255)                                                                                                                                tcCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTAAACACCTGGCGCTGCTCTGGTGATGc     >  2:703793/1‑140 (MQ=255)                                                                                                                                tcCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTAAACACCTGGCGCTGCTCTGGTGATGc     >  1:355421/1‑140 (MQ=255)                                                                                                                                tcCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTAAACACCTGGCGCTGCTCTGGTGATGc     <  1:218521/140‑1 (MQ=255)                                                                                                                                tcCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTAAACACCTGGCGCTGCTCTGGTGATGc     >  2:1359534/1‑140 (MQ=255)                                                                                                                                tcCTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTAAACACCTGGCGCTGCTCTGGTGATGc     >  2:218521/1‑140 (MQ=255)                                                                                                                                 ccTGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTAAACACCTGGCGCTGCTCTGGTGATGCg    <  2:1251743/140‑1 (MQ=255)                                                                                                                                   tGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTAAACACCTGGCGCTGCTCTGGTGATGCGGa  <  2:786289/140‑1 (MQ=255)                                                                                                                                   tGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTAAACACCTGGCGCTGCTCTGGTGATGCGGa  <  1:957666/140‑1 (MQ=255)                                                                                                                                   tGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTAAACACCTGGCGCTGCTCTGGTGATGCGGa  <  2:895541/140‑1 (MQ=255)                                                                                                                                   tGAAATTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTAAACACCTGGCGCTGCTCTGGTGATGCGGa  <  1:1359534/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        TACGTCTGCAATTTACCTTTCCAGTCTTCTTGCTCCACGTTCAGAGAGACGTTCGCATACTGCTGACCGTTGCTCGTTATTCAGCCTGACAGTATGGTTACTGTCGTTTAGACGTTGTGGGCGGCTCTCCTGAACTTTCTCCCGAAAAACCTGACGTTGTTCAGGTGATGCCGATTGAACACGCTGGCGGGCGTTATCACGTTGCTGTTGATTCAGTGGGCGCTGCTGTACTTTTTCCTTAAACACCTGGCGCTGCTCTGGTGATGCGGA  >  NC_000913/129395‑129664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑