Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,731,280	T→C	intergenic (‑123/+320)	rrsG ← / ← clpB	16S ribosomal RNA/ClpB chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,731,280	0	T	C	100.0%	67.4 / NA	21	intergenic (‑123/+320)	rrsG/clpB	16S ribosomal RNA/ClpB chaperone
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/6);  total (15/6)

GCGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACC  >  NC_000913/2731238‑2731416                                           |                                                                                                                                         gCGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGCCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTg                                         <  1:1089914/140‑1 (MQ=18) gCGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGCCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTg                                         >  1:141881/1‑140 (MQ=18) gCGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGCCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTg                                         <  2:657808/140‑1 (MQ=18) gCGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGCCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTg                                         >  1:1641058/1‑140 (MQ=18) gCGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGCCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTg                                         >  1:808633/1‑140 (MQ=18) gCGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGCCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTg                                         >  1:844686/1‑140 (MQ=18)       ttAAGAATCCGTATCTTCGAGTGCCCACACAGATTGCCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtc                                   >  2:69630/1‑140 (MQ=255)       ttAAGAATCCGTATCTTCGAGTGCCCACACAGATTGCCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtc                                   <  2:679502/140‑1 (MQ=38)       ttAAGAATCCGTATCTTCGAGTGCCCACACAGATTGCCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtc                                   >  1:493512/1‑140 (MQ=38)         aaGAATCCGTATCTTCGAGTGCCCACACAGATTGCCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtctc                                 <  2:989574/140‑1 (MQ=255)                cGTATCTTCGAGTGCCCACACAGATTGCCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTgag                          <  2:525582/140‑1 (MQ=255)                      ttCGAGTGCCCACACAGATTGCCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATtctc                    >  1:1374106/1‑140 (MQ=255)                      ttCGAGTGCCCACACAGATTGCCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGATGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATtctc                    >  2:1226029/1‑140 (MQ=255)                        cGAGTGCCCACACAGATTGCCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAg                  >  1:1224032/1‑140 (MQ=255)                              cccACACAGATTGCCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAAc            >  2:451463/1‑140 (MQ=255)                              cccACACAGATTGCCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAAc            >  1:1505065/1‑140 (MQ=255)                              cccACACAGATTGCCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAAc            >  2:918130/1‑140 (MQ=255)                                      gATTGCCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGa    >  2:1672307/1‑140 (MQ=255)                                       aTTGCCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGAc   >  1:827407/1‑140 (MQ=255)                                        ttGCCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGAcc  <  2:141881/140‑1 (MQ=255)                                        ttGCCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGAcc  >  1:1265837/1‑140 (MQ=255)                                           |                                                                                                                                         GCGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACC  >  NC_000913/2731238‑2731416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑