Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	844,291	C→A	W397L (TGG→TTG)	ybiO ←	moderate conductance mechanosensitive channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	844,291	0	C	A	100.0%	255.7 / NA	72	W397L (TGG→TTG)	ybiO	moderate conductance mechanosensitive channel
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (39/33);  total (39/33)

AGCAACATTACCGCCACGCAGACTGTCAGAATACGCGCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACCCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATAAAATAGGC  >  NC_000913/844156‑844424                                                                                                                                        |                                                                                                                                      aGCAACATCACCGCCACGCAGACTGTCAGAATACGCGCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCg                                                                                                                                   <  2:1542957/140‑1 (MQ=255)    aaCATTACCGCCACGCAGACTGTCAGAATACGCGCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGa                                                                                                                                <  1:347616/140‑1 (MQ=255)    aaCATTACCGCCACGCAGACTGTCAGAATACGCGCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGa                                                                                                                                <  1:191980/140‑1 (MQ=255)        ttACCGCCACGCAGACTGTCAGAATACGCGCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCgg                                                                                                                                  <  2:740739/134‑1 (MQ=255)        ttACCGCCACGCAGACTGTCAGAATACGCGCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCgg                                                                                                                                  >  1:740739/1‑134 (MQ=255)        ttACCGCCACGCAGACTGTCAGAATACGCGCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAAc                                                                                                                            >  1:1256659/1‑140 (MQ=255)        ttACCGCCACGCAGACTGTCAGAATACGCGCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAAc                                                                                                                            >  2:436812/1‑140 (MQ=255)           ccGCCACGCAGACTGTCAGAATACGCGCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATa                                                                                                                         >  1:314/1‑140 (MQ=255)                aCGCAGACTGTCAGAATACGCGCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGa                                                                                                                    >  2:321649/1‑140 (MQ=255)                 cGCAGACTGTCAGAATACGCGCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGaa                                                                                                                   >  2:503965/1‑140 (MQ=255)                 cGCAGACTGTCAGAATACGCGCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGaa                                                                                                                   >  1:528599/1‑140 (MQ=255)                 cGCAGACTGTCAGAATACGCGCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGaa                                                                                                                   >  2:86318/1‑140 (MQ=255)                      aCTGTCAGAATACGCGCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGaaacaaa                                                                                                              <  2:1222779/140‑1 (MQ=255)                      aCTGTCAGAATACGCGCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGaaacaaa                                                                                                              <  2:24666/140‑1 (MQ=255)                        tGTCAGAATACGCGCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACg                                                                                                            >  2:1461267/1‑140 (MQ=255)                        tGTCAGAATACGCGCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACg                                                                                                            <  1:503965/140‑1 (MQ=255)                             gAATACGCGCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCg                                                                                                       <  2:1452064/140‑1 (MQ=255)                             gAATACGCGCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCg                                                                                                       <  2:1334627/140‑1 (MQ=255)                                  cgcgCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATAcc                                                                                                  >  2:533163/1‑140 (MQ=255)                                  cgcgCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATAcc                                                                                                  >  2:220094/1‑140 (MQ=255)                                    cgcCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGTTGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCg                                                                                                       >  2:1501495/1‑133 (MQ=255)                                    cgcCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTg                                                                                                         <  2:261743/131‑1 (MQ=255)                                    cgcCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTg                                                                                                         >  1:261743/1‑131 (MQ=255)                                    cgcCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCg                                                                                                       <  1:1501495/133‑1 (MQ=255)                                    cgcCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCaa                                                                                                >  2:1649823/1‑140 (MQ=255)                                               gcgcCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAgg                                                                                     <  1:321649/140‑1 (MQ=255)                                               gcgcCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAgg                                                                                     <  1:1649823/140‑1 (MQ=255)                                               gcgcCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAgg                                                                                     <  2:1215489/140‑1 (MQ=255)                                                    gccgACAGCCAGCCATTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTgcg                                                                                <  2:918500/140‑1 (MQ=255)                                                     ccgACAGCCAGCCATTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTgcgc                                                                               <  2:314/140‑1 (MQ=255)                                                      cgACAGCCAGCCATTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCa                                                                              <  1:86318/140‑1 (MQ=255)                                                              agccaTTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCa                                                                      <  2:1361066/140‑1 (MQ=255)                                                               gccaTTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCa                                                                      >  1:987083/1‑139 (MQ=255)                                                               gccaTTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCa                                                                      <  2:987083/139‑1 (MQ=255)                                                                 caTTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCAccc                                                                   <  1:383521/140‑1 (MQ=255)                                                                  aTTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTgcg                                                                                >  1:1571840/1‑126 (MQ=255)                                                                  aTTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTgcg                                                                                <  2:1571840/126‑1 (MQ=255)                                                                          gTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACCCATCATAAAt                                                          >  2:656248/1‑140 (MQ=255)                                                                              ttGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACCCATCATAAATTTCa                                                      <  1:220094/140‑1 (MQ=255)                                                                                gCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACCCATCATAAATTTCAgg                                                    >  1:87544/1‑140 (MQ=255)                                                                                  aGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACCCATCATAAATTTCAGGCt                                                  <  2:528599/140‑1 (MQ=255)                                                                                   gCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACCCATCATAAATTTCAGGCTg                                                 <  2:657410/140‑1 (MQ=255)                                                                                   gCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACCCATCATAAATTTCAGGCTg                                                 <  1:237046/140‑1 (MQ=255)                                                                                      ccGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACCCATCATAAATTTCAGGCTGTTg                                              >  2:581576/1‑140 (MQ=255)                                                                                            aGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACCCATCATAAATTTCAGGCTGTTGCCCGGa                                        <  1:656248/140‑1 (MQ=255)                                                                                              ttACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACCCATCATAAATTTCAGGCTGTTGCCCGGATc                                      >  1:36173/1‑140 (MQ=255)                                                                                                   cTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACCCATCATAAATTTCAGGCTGTTGCCCGGATCGaaca                                 >  1:167015/1‑140 (MQ=255)                                                                                                      aGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACCCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACg                              >  1:1082408/1‑140 (MQ=255)                                                                                                      aGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACCCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACg                              >  2:1485285/1‑140 (MQ=255)                                                                                                        tATGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACCCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACgaa                            >  2:53074/1‑140 (MQ=255)                                                                                                         aTGTGGCGAGAGAGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCAGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACCCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACgaaa                           <  2:1256659/140‑1 (MQ=255)                                                                                                                gagagaGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACCCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGaaaaa                    >  2:1622282/1‑140 (MQ=255)                                                                                                                gagagaGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACCCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGaaaaa                    >  2:362731/1‑140 (MQ=255)                                                                                                                gagagaGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACCCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGaaaaa                    >  1:725773/1‑140 (MQ=255)                                                                                                                gagagaGTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACCCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGaaaaa                    >  2:146781/1‑140 (MQ=255)                                                                                                                   agagTGCTGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACCCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAg                 <  2:1290002/140‑1 (MQ=255)                                                                                                                   agagTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACCCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAg                 <  1:362731/140‑1 (MQ=255)                                                                                                                   agagTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACCCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAg                 <  1:472119/140‑1 (MQ=255)                                                                                                                   agagTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACCCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAg                 <  2:87544/140‑1 (MQ=255)                                                                                                                    gagTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACCCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGc                >  2:136061/1‑140 (MQ=255)                                                                                                                     agTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACCCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCa               >  1:1173017/1‑140 (MQ=255)                                                                                                                     agTGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACCCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCa               >  2:4861/1‑140 (MQ=255)                                                                                                                       tGATGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACCCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACg             >  1:548110/1‑140 (MQ=255)                                                                                                                         aTGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACCCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAg                 <  2:1281892/134‑1 (MQ=255)                                                                                                                         aTGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACCCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAg                 >  1:1281892/1‑134 (MQ=255)                                                                                                                         aTGGTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACCCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGAt           >  2:1108188/1‑140 (MQ=255)                                                                                                                            gTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACCCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATaaa        >  2:274050/1‑140 (MQ=255)                                                                                                                            gTTTTCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACCCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATaaa        >  1:586342/1‑140 (MQ=255)                                                                                                                             ttttCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACCCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATaaaa       >  2:1493965/1‑140 (MQ=255)                                                                                                                               ttCGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACCCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATAAAATa     >  1:734503/1‑140 (MQ=255)                                                                                                                                 cGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACCCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATAAAAt      >  2:1372092/1‑137 (MQ=255)                                                                                                                                 cGCCAGCAAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACCCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATAAAAt      <  1:1372092/137‑1 (MQ=255)                                                                                                                                  gccagcaaGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACCCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATAAAATAGgc  <  1:136061/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                      AGCAACATTACCGCCACGCAGACTGTCAGAATACGCGCCGTTTTCAGCGCCGCCGACAGCCAGCCATTCAACCGTTTTTGCAGCTCCGGATAGTTACGCTGAGTATGTGGCGAGAGAGTGATGGTTTTCGCCAGCCAGCGGGAAAACATACCGGAAACAAACGCTGCGATACCAATAATCGCCAGGCTGCGCACCGTTGCACCCATCATAAATTTCAGGCTGTTGCCCGGATCGAACAACGAAAAGAAAAACAGCACGATAAAATAGGC  >  NC_000913/844156‑844424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑