Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,825,0610GA34.7% 68.1 / 27.5 49intergenic (‑124/+79)ves/spyHutD family protein Ves/ATP‑independent periplasmic chaperone
Reads supporting (aligned to +/- strand):  ref base G (30/2);  new base A (0/17);  total (30/19)
Fisher's exact test for biased strand distribution p-value = 2.63e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCA  >  NC_000913/1824922‑1825200
                                                                                                                                           |                                                                                                                                           
tatCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGagaaaag                                                                                                                                             >  1:336483/1‑140 (MQ=255)
        tttcccATCCTCGCTCCGGGCCCTTTTTTTTTATTGTTATCGTTCAGGTACCGGGGGATGGTTATGGGCATCGGGGGTGTTTTACGTTAGGGGTGAGCGGTTTTTTTTTACAACTGTTGGAGAAGAAAAAAAAAAAccgc                                                                                                                                     <  2:221961/136‑1 (MQ=255)
        tATTCCATCCTCGCCCCCGCCCTTTTTTTTTCTTTGTTTTCGTTCAGGTAACGGGGGATGTTTATCGGCATCTGGGGTGATTTACGTTAGTGGTGATCGGGTTTTTTTTACAACTGTTTGAGAAGAAAAAAAAAAAccgc                                                                                                                                     <  2:336483/140‑1 (MQ=255)
           tCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGa                                                                                                                                  >  1:242424/1‑140 (MQ=255)
           tCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGa                                                                                                                                  >  1:300760/1‑140 (MQ=255)
           tCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGa                                                                                                                                  >  1:1354188/1‑140 (MQ=255)
           tCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGa                                                                                                                                  >  1:957935/1‑140 (MQ=255)
             cATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATc                                                                                                                                >  2:1872128/1‑140 (MQ=255)
               tACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCt                                                                                                                              >  1:415167/1‑140 (MQ=255)
                  tCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTc                                                                                                                           >  1:2155677/1‑140 (MQ=255)
                  tCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTATTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTc                                                                                                                           >  1:1014013/1‑140 (MQ=255)
                    ccccccGCCCCTTTTTTTTCATTTTTATCGTTCAGGTAACGGGGGATGGTTATCGGCAACTGGGGTGATTTACTTTGGTGGTGATCGGTTTTTTTTTACAACTTTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCa                                                                                                                         <  2:1507241/137‑1 (MQ=255)
                     cccccGCCCCGTTTTTTTCATTGTTATCGTTCAGGTACCCGTGGTTGTTTATCGGCAACTGGGGTGTTTTACTTTGGTGGTGATCGGTTTTTTTTTACACTTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCAc                                                                                                                        <  2:415167/138‑1 (MQ=255)
                      tCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCAcc                                                                                                                       >  2:968785/1‑140 (MQ=255)
                       cccGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAGAAGAAAACCGCCGATCCTGTCCACCg                                                                                                                      >  1:1896143/1‑140 (MQ=255)
                         cgccctgTTCTTTTCATTTTTACTGTCCGGGTACCGGGGGAGGGTTATCGGCAACTGGGGTGTTTTGCGTTAGTGGGGACCGGCTTTTTTTTACACTTGTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCa                                                                                                                    <  2:119445/134‑1 (MQ=255)
                         cGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCa                                                                                                                    >  2:988088/1‑140 (MQ=255)
                          ggCCCGTTTTTTTCATTGTTATCGTTCAGGTACCGGTGGATGTTTATCGGCAACTGGGGTGATTTACGTTGGTGGTGATCAGTTTTTTTTTACAACTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCAt                                                                                                                   <  2:1957013/140‑1 (MQ=255)
                          gccccGTTTTTTTATTTGTTATCGTTCAGGTAACGGTGGATGGTTATCGGCAACTGGGGTGTTTTACGTTAGTGGTGATCAGGTTTTTTTTACACCTTTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCAt                                                                                                                   <  2:1947678/138‑1 (MQ=255)
                            agagaatttttCTTTGTTATCTTTCAGGAAACCGTGGATGTTTTCCGGCAACGGGGGTGATTTACGTGGGTGGTGATCGGTCTTTTTTAACAATTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTa                                                                                                                 <  2:2253745/133‑1 (MQ=255)
                                ttCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGc                                                                                                             >  1:2136657/1‑140 (MQ=255)
                                ttCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGc                                                                                                             >  1:1204868/1‑140 (MQ=255)
                                ttCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGc                                                                                                             >  1:1957013/1‑140 (MQ=255)
                                ttCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGc                                                                                                             >  2:839107/1‑140 (MQ=255)
                                ttCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGc                                                                                                             >  1:1507241/1‑140 (MQ=255)
                                ttCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTAAAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGc                                                                                                             >  1:1042392/1‑140 (MQ=255)
                                      ttaTTTTTATCGTTCAGGTACCCGGGGATGTTTATCGGCAACTGGGGTGTTTTACGTTAGTGGTGATCAGTTTTTTTTAACAATTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTa                                                                                                       <  2:957935/138‑1 (MQ=255)
                                             tATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACa                                                                                                >  2:2343669/1‑140 (MQ=255)
                                                    agggTAACGGTGGATGGTTATCGGCAATGGGGGTGTTTTACGTTGGTGGTGATCAGTTTTTTTTAACAACTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcgg                                                                                         <  2:2068211/138‑1 (MQ=255)
                                                                     ttATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTg                                                                        >  2:901030/1‑140 (MQ=255)
                                                                      tATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGGCTTTAGttctgtct                                                                       >  1:1555142/1‑134 (MQ=255)
                                                                        tCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCt                                                                     >  1:1271990/1‑140 (MQ=255)
                                                                         cGGCACCTGGGGTTATTTACGTTAGTGGTGATCAGGTTTTTTTTACAACTGTTTGAGAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTg                                                                    <  2:1271990/140‑1 (MQ=255)
                                                                          ggCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGa                                                                   >  2:1615325/1‑140 (MQ=255)
                                                                           gCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCTCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGaa                                                                  <  2:1791849/140‑1 (MQ=255)
                                                                              cctggggTGTTTTACTTTATTGTTGATCAGTTTTTTTTAACAATTGTTTGAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGa                                                               <  2:1354188/136‑1 (MQ=255)
                                                                               cTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGat                                                              >  1:2112383/1‑140 (MQ=255)
                                                                                               tAGTGGTAATCGGGTTTTTTTTACAATTGTTAGAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAg                                              <  2:1229864/140‑1 (MQ=255)
                                                                                                  tggtgATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCa                                           >  1:739849/1‑140 (MQ=255)
                                                                                                       aTCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAttttt                                      >  1:1229864/1‑139 (MQ=255)
                                                                                                          gggttttttttACAGTTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCt                                   <  2:300760/136‑1 (MQ=255)
                                                                                                            gttttttttACAACTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCttt                                 <  1:1872128/138‑1 (MQ=255)
                                                                                                            gCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCttt                                 >  1:768963/1‑140 (MQ=255)
                                                                                                            gCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCttt                                 >  1:1408179/1‑140 (MQ=255)
                                                                                                                      cAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGa                       >  1:580568/1‑140 (MQ=255)
                                                                                                                        attttttGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACg                     <  1:968785/136‑1 (MQ=255)
                                                                                                                         ttGTTTGAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGt                    <  1:839107/139‑1 (MQ=255)
                                                                                                                          tGTTTGAGAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGtt                   <  1:2343669/140‑1 (MQ=255)
                                                                                                                            tttGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTTCCGCTGGACGTTCt                 >  1:488811/1‑140 (MQ=255)
                                                                                                                            tttGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCt                 >  1:1615780/1‑140 (MQ=255)
                                                                                                                                    aaaaaaaaaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACg         <  2:1408179/137‑1 (MQ=255)
                                                                                                                                     aaaaaaaaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGc        <  2:1071612/138‑1 (MQ=255)
                                                                                                                                           gaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCa  <  2:1042392/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
TATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCA  >  NC_000913/1824922‑1825200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑