BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,518,027	G→T	intergenic (‑195/+14)	pdeA ← / ← alaX	putative c‑di‑GMP phosphodiesterase PdeA/tRNA‑Ala

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,518,027	0	G	T	100.0%	496.5 / NA	138	intergenic (‑195/+14)	pdeA/alaX	putative c‑di‑GMP phosphodiesterase PdeA/tRNA‑Ala
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (65/73); total (65/73)

Only 100 of 139 total aligned reads displayed.

ATCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAG  >  NC_000913/2517891‑2518163

aTCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAgg                                                                                                                                       >  1:711663/1‑140 (MQ=255)

 tCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAggg                                                                                                                                      >  2:568647/1‑140 (MQ=255)

  cgcgAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGt                                                                                                                                     <  1:1991911/140‑1 (MQ=255)

     gAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTgg                                                                                                                                  <  2:1105907/140‑1 (MQ=255)

              aGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTtggt                                                                                                                         <  2:2038283/140‑1 (MQ=255)

               gggggCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTtggtg                                                                                                                        <  2:1449562/140‑1 (MQ=255)

                 gggCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGa                                                                                                                      >  1:327539/1‑140 (MQ=255)

                  ggCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAg                                                                                                                     >  1:2384994/1‑140 (MQ=255)

                  ggCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAg                                                                                                                     <  2:2384994/140‑1 (MQ=255)

                  ggCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAg                                                                                                                     <  1:762230/140‑1 (MQ=255)

                   gCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAGAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGc                                                                                                                    >  2:760018/1‑140 (MQ=255)

                        ccTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGc                                                                                                               >  1:2287124/1‑140 (MQ=255)

                        ccTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGc                                                                                                               >  2:1136933/1‑140 (MQ=255)

                                cGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGa                                                                                                       <  2:711663/140‑1 (MQ=255)

                                    ttttCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCg                                                                                                   <  2:1586131/140‑1 (MQ=255)

                                    ttttCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCg                                                                                                   <  2:1788536/140‑1 (MQ=255)

                                      ttCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGc                                                                                                  <  2:1290831/139‑1 (MQ=255)

                                      ttCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGc                                                                                                  >  1:1290831/1‑139 (MQ=255)

                                      ttCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCt                                                                                                 <  2:2056386/140‑1 (MQ=255)

                                         aTCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGAc                                                                                              <  1:1458986/140‑1 (MQ=255)

                                          tCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGAcc                                                                                             <  2:327539/140‑1 (MQ=255)

                                          tCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGAcc                                                                                             <  2:904599/140‑1 (MQ=255)

                                                 atgCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGca                                                                                      <  2:1720233/140‑1 (MQ=255)

                                                 atgCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTAAAGCTAGGGTTGGAAATTTGGTGGAGTTAAGCGGGATCGAACCGCTGACCTCTTGca                                                                                      <  2:1089235/140‑1 (MQ=255)

                                                     tttCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGcatgcc                                                                                  <  2:869085/140‑1 (MQ=255)

                                                     tttCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGcatgcc                                                                                  >  1:1367682/1‑140 (MQ=255)

                                                       tCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGcatgccat                                                                                >  1:897291/1‑140 (MQ=255)

                                                         aCCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGcatgccatgc                                                                              >  1:271014/1‑140 (MQ=255)

                                                         aCCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGcatgccatgc                                                                              >  1:1725090/1‑140 (MQ=255)

                                                         aCCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGca                                                                                      >  2:770836/1‑132 (MQ=255)

                                                             ggcggcACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACctct                                                                                          <  1:163801/124‑1 (MQ=255)

                                                             ggcggcACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACctct                                                                                          >  2:163801/1‑124 (MQ=255)

                                                             ggcggcACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAgc                                                                          >  2:1705026/1‑140 (MQ=255)

                                                             ggcggcACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAgc                                                                          >  1:2033998/1‑140 (MQ=255)

                                                             ggcggcACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAgc                                                                          <  2:2033998/140‑1 (MQ=255)

                                                              gcggcACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAgcg                                                                         >  1:135904/1‑140 (MQ=255)

                                                              gcggcACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAgcg                                                                         <  2:1288124/140‑1 (MQ=255)

                                                              gcgacACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAgcg                                                                         >  2:450747/1‑140 (MQ=255)

                                                                ggcACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGct                                                                       >  1:653036/1‑140 (MQ=255)

                                                                  cACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGctct                                                                     <  2:897291/140‑1 (MQ=255)

                                                                   aCTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAGGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCtctc                                                                    >  1:1420926/1‑140 (MQ=255)

                                                                   aCTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAATAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCtctc                                                                    >  1:1255526/1‑140 (MQ=255)

                                                                    cTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTcc                                                                   <  2:1060784/140‑1 (MQ=255)

                                                                       aCCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAg                                                                <  2:909639/140‑1 (MQ=255)

                                                                         ccGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCt                                                              <  2:1891090/140‑1 (MQ=255)

                                                                         ccGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCt                                                              <  1:1589414/140‑1 (MQ=255)

                                                                          cGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTg                                                             <  1:1436872/140‑1 (MQ=255)

                                                                             aCGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGc                                                          <  2:1818745/140‑1 (MQ=255)

                                                                             aCGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGc                                                          <  2:1577751/140‑1 (MQ=255)

                                                                               gTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCta                                                        <  1:757706/140‑1 (MQ=255)

                                                                               gTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCta                                                        <  1:1429743/140‑1 (MQ=255)

                                                                                 aGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGctc                                                                      >  2:593453/1‑124 (MQ=255)

                                                                                 aGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGctc                                                                      <  1:593453/124‑1 (MQ=255)

                                                                                 aGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCtata                                                      >  1:2385119/1‑140 (MQ=255)

                                                                                       tATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCt                                                              >  1:1911720/1‑126 (MQ=255)

                                                                                       tATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGccc                                                  <  1:926314/138‑1 (MQ=255)

                                                                                       tATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGccc                                                  >  2:926314/1‑138 (MQ=255)

                                                                                       tATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGccc                                                  <  2:1120614/138‑1 (MQ=255)

                                                                                       tATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCa                                                >  2:1135506/1‑140 (MQ=255)

                                                                                        aTTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCAc                                               <  2:271014/140‑1 (MQ=255)

                                                                                         ttACAAAAGCGGCAAGAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACg                                              <  1:760018/140‑1 (MQ=255)

                                                                                         ttACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCt                                                              <  2:2048456/124‑1 (MQ=255)

                                                                                         ttACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCt                                                              >  1:2048456/1‑124 (MQ=255)

                                                                                         ttACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACg                                              <  2:1725090/140‑1 (MQ=255)

                                                                                          tACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGa                                             >  2:2334988/1‑140 (MQ=255)

                                                                                          tACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGa                                             <  2:1367682/140‑1 (MQ=255)

                                                                                                aGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGtt                                       <  2:653036/140‑1 (MQ=255)

                                                                                                aGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGtt                                       <  2:251485/140‑1 (MQ=255)

                                                                                                aGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGtt                                       <  1:450747/140‑1 (MQ=255)

                                                                                                 gCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATTGCCCCACg                                              <  2:1871883/132‑1 (MQ=255)

                                                                                                 gCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCAc                                               >  1:527673/1‑131 (MQ=255)

                                                                                                 gCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACg                                              >  1:1871883/1‑132 (MQ=255)

                                                                                                 gCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACg                                              >  1:1802521/1‑132 (MQ=255)

                                                                                                 gCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGttt                                      >  1:1058467/1‑140 (MQ=255)

                                                                                                 gCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGttt                                      >  2:428602/1‑140 (MQ=255)

                                                                                                 gCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGttt                                      >  2:776610/1‑140 (MQ=255)

                                                                                                 gCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGttt                                      >  2:1605708/1‑140 (MQ=255)

                                                                                                 gCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGttt                                      >  2:1391890/1‑140 (MQ=255)

                                                                                                 gCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGttt                                      >  1:903664/1‑140 (MQ=255)

                                                                                                 gCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGttt                                      >  1:1707684/1‑140 (MQ=255)

                                                                                                 gCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGttt                                      <  1:1605708/140‑1 (MQ=255)

                                                                                                 gCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGttt                                      >  1:1196571/1‑140 (MQ=255)

                                                                                                 gCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTAACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGttt                                      >  1:1539154/1‑140 (MQ=255)

                                                                                                 gCGGCAAAAAGCAGAGAC‑AAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGccc                                                  <  2:1973339/127‑1 (MQ=255)

                                                                                                  cGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTa                                     <  2:540805/140‑1 (MQ=255)

                                                                                                      aaaaaGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTa                                 <  1:2400405/140‑1 (MQ=255)

                                                                                                       aaaaGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTAc                                <  2:2368000/140‑1 (MQ=255)

                                                                                                       aaaaGCAGAGACAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTAc                                <  1:984414/140‑1 (MQ=255)

                                                                                                                 aCAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCt                      <  1:1725715/140‑1 (MQ=255)

                                                                                                                  cAAAAAACCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGtt                                       <  1:1068238/122‑1 (MQ=255)

                                                                                                                        aCCCCCGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCa               <  1:1581180/140‑1 (MQ=255)

                                                                                                                           cccGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCaaaa            >  2:2210930/1‑140 (MQ=255)

                                                                                                                           cccGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCaaaa            >  2:173171/1‑140 (MQ=255)

                                                                                                                           cccGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCaaaa            >  2:968059/1‑140 (MQ=255)

                                                                                                                           cccGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCaaaa            >  2:970922/1‑140 (MQ=255)

                                                                                                                             cGCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAAtt          >  2:2370964/1‑140 (MQ=255)

                                                                                                                              gCTTTGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAAttt         >  1:2340360/1‑140 (MQ=255)

                                                                                                                                tttGCAGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAACGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTtgg       <  1:1112890/140‑1 (MQ=255)

                                                                                                                                     aGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAg  <  2:135904/140‑1 (MQ=255)

                                                                                                                                     aGCTAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAg  <  1:1659955/140‑1 (MQ=255)

ATCGCGAAGCAAACAGGGGGCCAGCCTGTTGCCGATTTTTCATCCAATGATGCTTTCACCAGGCGGCACTAACCCGGACGTAGACTGTATTACAAAAGCGGCAAAAAGCAGAGACAAAAAACCCCCGCTTTGCAGCGAGGGTTGGAAATTTGGTGGAGCTAAGCGGGATCGAACCGCTGACCTCTTGCATGCCATGCAAGCGCTCTCCCAGCTGAGCTATAGCCCCACGATGCGTTTACGTACCAAGTTTGCTGGGTGCAAAATTTGGTGGAG  >  NC_000913/2517891‑2518163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`