Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,526,395	C→A	V154V (GTG→GTT)	yfeR ←	putative LysR‑type DNA‑binding transcriptional regulator YfeR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,526,395	0	C	A	100.0%	347.8 / NA	98	V154V (GTG→GTT)	yfeR	putative LysR‑type DNA‑binding transcriptional regulator YfeR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (39/59);  total (39/59)

GACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCCACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCACTGCTGCGGACGATCGTGCAGGACAAACTGAATATCTGGATAGCGGCGATGGCTTTCAGCAATGCATTGC  >  NC_000913/2526258‑2526530                                                                                                                                          |                                                                                                                                        gACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAAc                                                                                                                                       <  1:2365670/140‑1 (MQ=255) gACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAAc                                                                                                                                       <  2:1689670/140‑1 (MQ=255) gACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAAc                                                                                                                                       <  2:2184299/140‑1 (MQ=255) gACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATCGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAAc                                                                                                                                       <  2:1011463/140‑1 (MQ=255)       tAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGcc                                                                                                                                 <  1:175382/140‑1 (MQ=255)        aaTCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCt                                                                                                                                <  2:870442/140‑1 (MQ=255)        aaTCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCt                                                                                                                                <  1:965256/140‑1 (MQ=255)        aaTCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCt                                                                                                                                <  1:1446459/140‑1 (MQ=255)              gCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAAc                                                                                                                                       >  1:6622/1‑127 (MQ=255)                aaCACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGAt                                                                                                                        >  1:1174682/1‑140 (MQ=255)                  cacCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGTTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGa                                                                                                                      >  2:1827975/1‑140 (MQ=255)                    ccAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACg                                                                                                                    <  2:606586/140‑1 (MQ=255)                    ccAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACg                                                                                                                    >  2:393123/1‑140 (MQ=255)                       aTTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATg                                                                                                                 >  2:541730/1‑140 (MQ=255)                       aTTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATg                                                                                                                 <  1:1733974/140‑1 (MQ=255)                       aTTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATg                                                                                                                 >  1:979982/1‑140 (MQ=255)                         ttCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGcc                                                                                                               <  1:880998/140‑1 (MQ=255)                            gCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTTGATCGATGACGATGCCaaa                                                                                                            >  1:1509618/1‑140 (MQ=255)                                 cTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGa                                                                                                       <  2:1108002/140‑1 (MQ=255)                                   gTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACa                                                                                                     <  2:1212869/140‑1 (MQ=255)                                      aTGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACAtct                                                                                                  <  1:505920/140‑1 (MQ=255)                                       tgcctgccAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACAtctc                                                                                                 >  1:1228429/1‑140 (MQ=255)                                        gcctgccAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTAGGCCTGGATCGATGACGATGCCAAAATCGACATCTcc                                                                                                <  2:54790/140‑1 (MQ=255)                                        gcctgccAGGGTACATAATCTCCCACGGCCAAACCGCTATCGCGATGAAAAAGCAAAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGAGGTTGCAAAAATCGACATCTcc                                                                                                <  2:858338/140‑1 (MQ=255)                                          ctgccAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAAt                                                                                                          >  2:1767600/1‑128 (MQ=255)                                          ctgccAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAAt                                                                                                          <  1:1767600/128‑1 (MQ=255)                                          ctgccAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCt                                                                                              >  2:2073641/1‑140 (MQ=255)                                          ctgccAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCt                                                                                              >  2:2262565/1‑140 (MQ=255)                                           tgccAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATTGATGACGATGCCAAAATCGACATCTCCCTg                                                                                             >  1:743572/1‑140 (MQ=255)                                           tgccAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTg                                                                                             >  1:955198/1‑140 (MQ=255)                                            gccAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCa                                                                                                              >  1:2366680/1‑122 (MQ=255)                                            gccAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCa                                                                                                              <  2:2366680/122‑1 (MQ=255)                                            gccAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTgg                                                                                            >  2:2096117/1‑140 (MQ=255)                                            gccAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTgg                                                                                            >  1:1119589/1‑140 (MQ=255)                                            gccAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTgg                                                                                            >  1:576043/1‑140 (MQ=255)                                            gccAGGGTACATAATCCTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTgg                                                                                            >  2:1558635/1‑140 (MQ=255)                                              cAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCg                                                                                          <  2:594287/140‑1 (MQ=255)                                               aGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATTGATGACGATGCCAAAATCGACATCTCCCTGGCGa                                                                                         <  2:743572/140‑1 (MQ=255)                                                      aTAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACttt                                                                                  <  2:979982/140‑1 (MQ=255)                                                      aTAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACttt                                                                                  <  2:796061/140‑1 (MQ=255)                                                         aTCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCa                                                                               <  1:1370715/140‑1 (MQ=255)                                                         aTCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCa                                                                               <  1:105779/140‑1 (MQ=255)                                                           cTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCAtt                                                                             >  1:171782/1‑140 (MQ=255)                                                            ttCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTa                                                                            >  2:2365120/1‑140 (MQ=255)                                                            ttCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTa                                                                            >  1:816923/1‑140 (MQ=255)                                                            ttCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTa                                                                            >  2:258033/1‑140 (MQ=255)                                                             tCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCa                                                                               >  2:145319/1‑136 (MQ=255)                                                             tCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCa                                                                               <  1:145319/136‑1 (MQ=255)                                                             tCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTAc                                                                           >  1:2275307/1‑140 (MQ=255)                                                             tCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTAc                                                                           >  2:1320777/1‑140 (MQ=255)                                                             tCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTAc                                                                           >  1:402093/1‑140 (MQ=255)                                                               cACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCa                                                                               >  1:364415/1‑134 (MQ=255)                                                               cACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCa                                                                               <  2:364415/134‑1 (MQ=255)                                                               cACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTAccc                                                                         >  1:340288/1‑140 (MQ=255)                                                               cACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTAccc                                                                         >  2:1462571/1‑140 (MQ=255)                                                                   gCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTTGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCActg                                                                     <  2:1509618/140‑1 (MQ=255)                                                                   gCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCActg                                                                     <  1:1833034/140‑1 (MQ=255)                                                                   gCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCActg                                                                     <  1:2109998/140‑1 (MQ=255)                                                                   gCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCActg                                                                     <  2:1848775/140‑1 (MQ=255)                                                                      aaaGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCActgctg                                                                  <  1:1462571/140‑1 (MQ=255)                                                                        aGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCACTGCTGCg                                                                <  2:816923/140‑1 (MQ=255)                                                                        aGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCACTGCTGCg                                                                >  1:360260/1‑140 (MQ=255)                                                                             tATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCACTGCTGCGGACGa                                                           <  2:576043/140‑1 (MQ=255)                                                                                cgcgATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCACTGCTGCGGACGATCg                                                        <  1:2365120/140‑1 (MQ=255)                                                                                  cgATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCACTGCTGCGGACGATCGTg                                                      <  1:736323/140‑1 (MQ=255)                                                                                  cgATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCACTGCTGCGGACGATCGTg                                                      <  1:1320777/140‑1 (MQ=255)                                                                                      ggCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCACTGCTGCGGACGATCGTGCAgg                                                  <  2:955198/140‑1 (MQ=255)                                                                                      ggCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCACTGCTGCGGACGATCGTGCAgg                                                  <  2:171782/140‑1 (MQ=255)                                                                                         aaaGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCACTGCTGCg                                                                >  2:563114/1‑123 (MQ=255)                                                                                         aaaGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCACTGCTGCg                                                                <  1:563114/123‑1 (MQ=255)                                                                                         aaaGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCACTGCTGCGGACGATCGTGCAGGACa                                               <  2:2102703/140‑1 (MQ=255)                                                                                         aaaGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCACTGCTGCGGACGATCGTGCAGGACa                                               <  2:1119589/140‑1 (MQ=255)                                                                                           aGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCACTGCTGCGGACGATCGTGCAGGACaaa                                             >  2:1244336/1‑140 (MQ=255)                                                                                             cAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCACTGCTGCGGACGATCGTGCAGGACAAACt                                           <  1:59579/140‑1 (MQ=255)                                                                                              agaaagaaAGGCTCGGAAAGAATCGTTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCACTGCTGCGGACGATCGTGCAGGACAAACTg                                          <  1:1827975/140‑1 (MQ=255)                                                                                              agaaagaaAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCACTGCTGCGGACGATCGTGCAGGACAAACTg                                          <  2:402093/140‑1 (MQ=255)                                                                                              agaaagaaAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCACTGCTGCGGACGATCGTGCAGGACAAACTg                                          <  1:40030/140‑1 (MQ=255)                                                                                               gaaagaaaGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCACTGCTGCGGACGATCGTGCAGGACAAACTGa                                         <  1:2262565/140‑1 (MQ=255)                                                                                                 aagaaagGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCACTGCTGCGGACGATCGTg                                                      >  2:1298377/1‑125 (MQ=255)                                                                                                 aagaaagGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCACTGCTGCGGACGATCGTg                                                      <  1:1298377/125‑1 (MQ=255)                                                                                                 aagaaagGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCACTGCTGCGGACGATCGTGCAGGACAAACTGAat                                       >  1:240293/1‑140 (MQ=255)                                                                                                 aagaaagGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCACTGCTGCGGACGATCGTGCAGGACAAACTGAat                                       >  1:1454800/1‑140 (MQ=255)                                                                                                   gaaagGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCACTGCTGCGGACGATCGTGCAGGACAAACTGAatat                                     <  1:2344129/140‑1 (MQ=255)                                                                                                   gaaagGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCACTGCTGCGGACGATCGTGCAGGACAAACTGAatat                                     <  2:360260/140‑1 (MQ=255)                                                                                                   gaaagGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCACTGCTGCGGACGATCGTGCAGGACAAACTGAatat                                     <  2:340288/140‑1 (MQ=255)                                                                                                                 gAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCACTGCTGCGGACGATCGTGCAGGACAAACTGAATATCTGGATAGCGGCGa                       <  1:588996/140‑1 (MQ=255)                                                                                                                 gAATCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCACTGCTGCGGACGATCGTGCAGGACAAACTGAATATCTGGATAGCGGCGa                       <  2:1228429/140‑1 (MQ=255)                                                                                                                  aaTCGCTTCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCAACTGCTGCGGACGATCGTGCAGGACAAACTGAATATCTGGATAGCGGCGAt                      >  2:1051425/1‑140 (MQ=255)                                                                                                                        ttCACATTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCACTGCTGCGGACGATCGTGCAGGACAAACTGAATATCTGGATAGCGGCGATGGCttt                <  1:1558635/140‑1 (MQ=255)                                                                                                                           acaTTGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCACTGCTGCGGACGATCGTGCAGGACAAACTGAATATCTGGATAGCGGCGATGGCTTTCAg             <  1:541730/140‑1 (MQ=255)                                                                                                                               tGCAGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCACTGCTGCGGACGATCGTGCAGGACAAACTGAATATCTGGATAGCGGCGATGGCTTTCAGCAAt         >  2:281445/1‑140 (MQ=255)                                                                                                                                  aGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCACTGCTGCGGACGATCGTGCAGGACAAACTGAATATCTGGATAGCGGCGa                       <  2:2150812/123‑1 (MQ=255)                                                                                                                                  aGATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCACTGCTGCGGACGATCGTGCAGGACAAACTGAATATCTGGATAGCGGCGa                       >  1:2150812/1‑123 (MQ=255)                                                                                                                                   gATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATGCTTTCCATTACCCACTGCTGCGGACGATCGTGCAGGACAAACTGAATATCTGGATAGCG‑‑GATGGCTTTCAGCAATGCATTg   <  1:393123/140‑1 (MQ=255)                                                                                                                                   gATCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCACTGCTGCGGACGATCGTGCAGGACAAACTGAATATCTGGATAGCGGCGATGGCTTTCAGCAATGCAt     <  1:258033/140‑1 (MQ=255)                                                                                                                                    aTCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCACTGCTGCGGACGATCGTGCAGGACAAACTGAATATCTGGATAGCGGCGATGGCTTTCAGCAATGCAtt    <  2:1174682/140‑1 (MQ=255)                                                                                                                                    aTCGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCACTGCTGCGGACGATCGTGCAGGACAAACTGAATATCTGGATAGCGGCGATGGCTTTCAGCAATGCAtt    <  1:281445/140‑1 (MQ=255)                                                                                                                                      cGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCACTGCTGCGGACGATCGTGCAGGACAAACTGAATATCTGGATAGCGGCGATGGCTTTCAGCAATGCATTGc  <  2:240293/140‑1 (MQ=255)                                                                                                                                      cGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCACTGCTGCGGACGATCGTGCAGGACAAACTGAATATCTGGATAGCGGCGATGGCTTTCAGCAATGCATTGc  >  1:2250839/1‑140 (MQ=255)                                                                                                                                      cGCCAACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCAACTGCTGCGGACGATCGTGCAGGACAAACTGAATATCTGGATAGCGGCGATGGCTTTCAGCAATGCATTGc  <  1:1051425/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        GACGCGTAATCCTGCAACACCAATTTCGCTCCCTGTAATGCCTGCCAGGGTACATAATCTTCCACGGCCAAAGCGCTATCGCGATGGCAAAGCAGAAAGAAAGGCTCGGAAAGAATCGCTTCACATTGCAGATCGCCCACTGGGCCTGGATCGATGACGATGCCAAAATCGACATCTCCCTGGCGAATACTTTCCATTACCCACTGCTGCGGACGATCGTGCAGGACAAACTGAATATCTGGATAGCGGCGATGGCTTTCAGCAATGCATTGC  >  NC_000913/2526258‑2526530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑