Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,606,471 G→T L70L (CTG→CTT)  hyfE → hydrogenase 4 component E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,606,4710GT100.0% 378.2 / NA 106L70L (CTG→CTT) hyfEhydrogenase 4 component E
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (35/71);  total (35/71)

Only 100 of 109 total aligned reads displayed.

AAAGCTATCGCCTGTCATGCGGATTTTACGCCTGCCAGTCACTGGTGCTGGTGTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTGCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGTCCGGCAATGATGGCACTGCTCGCGGCGTTAATTGTCCTGCTTTGCGCATTTGTCGTTCAGC  >  NC_000913/2606335‑2606604
                                                                                                                                        |                                                                                                                                     
aaaGCTATCGCCTGTCATGCGGATTTTACGCCTGCCAGTCACTGGTGCTGGTGTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGcttctg                                                                                                                                    <  2:1028285/140‑1 (MQ=255)
aaaGCTATCGCCTGTCATGCGGATTTTACGCCTGCCAGTCACTGGTGCTGGTGTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGcttctg                                                                                                                                    <  1:74943/140‑1 (MQ=255)
  aGCTATCGCCTGTCATGCGGATTTTACGCCTGCCAGTCACTGGTGCTGGTGTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGt                                                                                                                                  <  2:1576639/140‑1 (MQ=255)
   gCTATCGCCTGTCATGCGGATTTTACGCCTGCCAGTCACTGGTGCTGGTGTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTa                                                                                                                                 <  2:1518950/140‑1 (MQ=255)
   gCTATCGCCTGTCATGCGGATTTTACGCCTGCCAGTCACTGGTGCTGGTGTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTa                                                                                                                                 <  2:1459413/140‑1 (MQ=255)
    cTATCGCCTGTCATGCGGATTTTACGCCTGCCAGTCACTGGTGCTGGTGTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTAc                                                                                                                                >  1:1191353/1‑140 (MQ=255)
      aTCGCCTGTCATGCGGATTTTACGCCTGCCAGTCACTGGTGCTGGTGTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCg                                                                                                                              <  2:377416/140‑1 (MQ=255)
       tCGCCTGTCATGCGGATTTTACGCCTGCCAGTCACTGGTGCTGGTGTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGTTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGt                                                                                                                             <  2:1423277/140‑1 (MQ=255)
       tCGCCTGTCATGCGGATTTTACGCCTGCCAGTCACTGGTGCTGGTGTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGt                                                                                                                             <  1:621923/140‑1 (MQ=255)
       tCGCCTGTCATGCGGATTTTACGCCTGCCAGTCACTGGTGCTGGTGTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGt                                                                                                                             <  2:1115183/140‑1 (MQ=255)
            tGTCATGCGGATTTTACGCCTGCCAGTCACTGGTGCTGGTGTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATc                                                                                                                        <  1:1028794/140‑1 (MQ=255)
            tGTCATGCGGATTTTACGCCTGCCAGTCACTGGTGCTGGTGTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATc                                                                                                                        <  1:1859919/140‑1 (MQ=255)
            tGTCATGCGGATTTTACGCCTGCCAGTCACTGGTGCTGGTGTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATc                                                                                                                        <  1:779144/140‑1 (MQ=255)
            tGTCATGCGGATTTTACGCCTGCCAGTCACTGGTGCTGGTGTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATc                                                                                                                        <  1:489876/140‑1 (MQ=255)
            tGTCATGCGGATTTTACGCCTGCCAGTCACTGGTGCTGGTGTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATc                                                                                                                        <  1:1176185/140‑1 (MQ=255)
            tGTCATGCGGATTTTACGCCTGCCAGTCACTGGTGCTGGTGTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATc                                                                                                                        <  2:1528493/140‑1 (MQ=255)
              tCATGCGGATTTTACGCCTGCCAGTCACTGGTGCTGGTGTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGt                                                                                                                             <  2:693214/133‑1 (MQ=255)
              tCATGCGGATTTTACGCCTGCCAGTCACTGGTGCTGGTGTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGt                                                                                                                             >  1:693214/1‑133 (MQ=255)
                    ggATTTTACGCCTGCCAGTCACTGGTGCTGGTGTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTa                                                                                                                <  2:1018781/140‑1 (MQ=255)
                    ggATTTTACGCCTGCCAGTCACTGGTGCTGGTGTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTa                                                                                                                <  1:2084495/140‑1 (MQ=255)
                      aTTTTACGCCTGCCAGTCACTGGTGCTGGTGTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACg                                                                                                              <  1:2151658/140‑1 (MQ=255)
                        tttACGCCTGCCAGTCACTGGTGCTGGTGTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAAt                                                                                                                         >  1:2254281/1‑127 (MQ=255)
                        tttACGCCTGCCAGTCACTGGTGCTGGTGTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAAt                                                                                                                         <  2:2254281/127‑1 (MQ=255)
                        tttACGCCTGCCAGTCACTGGTGCTGGTGTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCt                                                                                                            >  2:164744/1‑140 (MQ=255)
                        tttACGCCTGCCAGTCACTGGTGCTGGTGTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCt                                                                                                            <  2:473507/140‑1 (MQ=255)
                        tttACGCCTGCCAGTCACTGGTGCTGGTGTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCt                                                                                                            <  2:1193225/140‑1 (MQ=255)
                           aCGCCTGCCAGTCACTGGTGCTGGTGTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCa                                                                                                         <  1:1266993/140‑1 (MQ=255)
                           aCGCCTGCCAGTCACTGGTGCTGGTGTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCa                                                                                                         <  2:1133558/140‑1 (MQ=255)
                                tGCCAGTCACTGGTGCTGGTGTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGaaa                                                                                                    >  2:1334017/1‑140 (MQ=255)
                                  ccAGTCACTGGTGCTGGTGTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACt                                                                                                                  <  2:1078332/124‑1 (MQ=255)
                                  ccAGTCACTGGTGCTGGTGTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACt                                                                                                                  >  1:1078332/1‑124 (MQ=255)
                                  ccAGTCACTGGTGCTGGTGTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAata                                                                                                  <  2:1727053/140‑1 (MQ=255)
                                  ccAGTCACTGGTGCTGGTGTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAata                                                                                                  <  2:550459/140‑1 (MQ=255)
                                  ccAGTCACTGGTGCTGGTGTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAata                                                                                                  <  2:2402543/140‑1 (MQ=255)
                                  ccAGTCACTGGTGCTGGTGTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAata                                                                                                  >  2:1534434/1‑140 (MQ=255)
                                  ccAGTCACTGGTGCTGGTGTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAata                                                                                                  <  2:1191353/140‑1 (MQ=255)
                                       cACTGGTGCTGGTGTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTccc                                                                                             <  1:594497/140‑1 (MQ=255)
                                       cACTGGTGCTGGTGTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTccc                                                                                             <  1:696790/140‑1 (MQ=255)
                                        aCTGGTGCTGGTGTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACg                                                                                                              <  2:1550744/122‑1 (MQ=255)
                                             tgctggtgTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAAc                                                                                       >  2:2229551/1‑140 (MQ=255)
                                             tgctggtgTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAAc                                                                                       >  1:2075717/1‑140 (MQ=255)
                                               ctggtgTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACAt                                                                                     >  2:393302/1‑140 (MQ=255)
                                                 ggtgTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATcc                                                                                   <  1:1334017/140‑1 (MQ=255)
                                                  gtgtCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATccc                                                                                  >  2:979934/1‑140 (MQ=255)
                                                    gtCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCgg                                                                                >  2:753720/1‑140 (MQ=255)
                                                    gtCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATT‑CCCAGAACATCCCGGa                                                                               >  1:1327792/1‑140 (MQ=255)
                                                      cTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGaa                                                                              <  2:2193356/140‑1 (MQ=255)
                                                       tATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGa                                                                                         >  1:69354/1‑128 (MQ=255)
                                                       tATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGa                                                                                         <  2:69354/128‑1 (MQ=255)
                                                       tATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGaaa                                                                             <  2:325928/140‑1 (MQ=255)
                                                       tATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGaaa                                                                             >  1:806844/1‑140 (MQ=255)
                                                       tATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGaaa                                                                             >  1:533615/1‑140 (MQ=255)
                                                          tttCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAg                                                                          <  2:2082799/140‑1 (MQ=255)
                                                            tCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCg                                                                        <  2:1102711/140‑1 (MQ=255)
                                                              gCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGtt                                                                      >  2:577338/1‑140 (MQ=255)
                                                              gCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGtt                                                                      >  2:1120291/1‑140 (MQ=255)
                                                                 aCTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTAtt                                                                   <  1:164744/140‑1 (MQ=255)
                                                                 aCTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTAtt                                                                   <  2:2075717/140‑1 (MQ=255)
                                                                     tctcGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGt                                                               <  2:2162254/140‑1 (MQ=255)
                                                                     tctcGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGt                                                               <  1:1120291/140‑1 (MQ=255)
                                                                     tctcGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGt                                                               <  2:2197249/140‑1 (MQ=255)
                                                                     tctcGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGt                                                               <  1:577338/140‑1 (MQ=255)
                                                                     tctcGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGt                                                               >  1:87227/1‑140 (MQ=255)
                                                                     tctcGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGt                                                               <  1:91270/140‑1 (MQ=255)
                                                                          tGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGTCCGGc                                                          >  2:1690366/1‑140 (MQ=255)
                                                                          tGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGTCCGGc                                                          <  1:2229551/140‑1 (MQ=255)
                                                                          tGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGTCCGGc                                                          >  1:1235541/1‑140 (MQ=255)
                                                                            ccTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGTCCGGCaa                                                        >  1:2120871/1‑140 (MQ=255)
                                                                             cTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGt                                                               >  1:683972/1‑132 (MQ=255)
                                                                             cTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGt                                                               <  2:683972/132‑1 (MQ=255)
                                                                               gTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGTCCGGc                                                          <  1:598173/135‑1 (MQ=255)
                                                                               gTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGTCCGGc                                                          >  2:598173/1‑135 (MQ=255)
                                                                               gTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGTCCGGCAatga                                                     >  1:938694/1‑140 (MQ=255)
                                                                                 tCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGTCCGGCAatgatg                                                   >  1:632528/1‑140 (MQ=255)
                                                                                          agCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGTCCGGCAATGATGGCACTGCTc                                          >  1:2403898/1‑140 (MQ=255)
                                                                                           gCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGTCCGGCAATGATGGCACTGCTcg                                         <  1:1927687/140‑1 (MQ=255)
                                                                                            cAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGTCCGGCAATGATGGCACTGCTcgc                                        <  2:2120871/140‑1 (MQ=255)
                                                                                            cAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGTCCGGCAATGATGGCACTGCTcgc                                        <  2:87227/140‑1 (MQ=255)
                                                                                            cAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGTCCGGCAATGATGGCACTGCTcgc                                        <  2:459032/140‑1 (MQ=255)
                                                                                            cAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGTCCGGCAATGATGGCACTGCTcgc                                        <  2:533615/140‑1 (MQ=255)
                                                                                            cAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGTCCGGCAATGATGGCACTGCTcgc                                        <  1:753720/140‑1 (MQ=255)
                                                                                              aCTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATT‑CCCAGAACATCCCGGAAAAAGCGTTATTCGGTCCGGCAATGATGGCACTGCTCgcggc                                     <  2:1327792/140‑1 (MQ=255)
                                                                                               ctgctgATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGTCCGGCAATGATGGCACTGCTCgcggc                                     <  1:290115/140‑1 (MQ=255)
                                                                                                   tgATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGTCCGGCAATGATGGCACTGCTCGCGGCGTTa                                 <  1:393302/140‑1 (MQ=255)
                                                                                                    gATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGTCCGGCAATGATGGCACTGCTCGCGGCGTTaa                                <  2:644171/140‑1 (MQ=255)
                                                                                                    gATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGTCCGGCAATGATGGCACTGCTCGCGGCGTTaa                                <  2:632528/140‑1 (MQ=255)
                                                                                                    gATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGTCCGGCAATGATGGCACTGCTCGCGGCGTTaa                                <  2:2328789/140‑1 (MQ=255)
                                                                                                    gATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGTCCGGCAATGATGGCACTGCTCGCGGCGTTaa                                <  2:938694/140‑1 (MQ=255)
                                                                                                    gATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGTCCGGCAATGATGGCACTGCTCGCGGCGTTaa                                >  2:1986956/1‑140 (MQ=255)
                                                                                                         ggTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGTCCGGCAATGATGGCACTGCTCGCGGCGTTAATTGTc                           <  1:1962148/140‑1 (MQ=255)
                                                                                                         ggTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGTCCGGCAATGATGGCACTGCTCGCGGCGTTAATTGTc                           <  1:462233/140‑1 (MQ=255)
                                                                                                          gTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGTCCGGCAATGATGGCACTGCTCGCGGCGTTAATTGTcc                          >  2:283008/1‑140 (MQ=255)
                                                                                                          gTCCGCCAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGTCCGGCAATGATGGCACTGCTCGCGGCGTTAATTGTcc                          >  1:118456/1‑140 (MQ=255)
                                                                                                               ccAGCGCCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGTCCGGCAATGATGGCACTGCTCGCGGCGTTAATTGTCCTGCtt                     >  2:148947/1‑140 (MQ=255)
                                                                                                                   cgcCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGTCCGGCAATGATGGCACTGCTCGCGGCGTTAATTGTCCTGCTTTgcg                 <  2:806844/140‑1 (MQ=255)
                                                                                                                    gcCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGTCCGGCAATGATGGCACTGCTCGCGGCGTTAATTGTCCTGCTTTgcgc                <  2:983678/140‑1 (MQ=255)
                                                                                                                    gcCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGTCCGGCAATGATGGCACTGCTCGCGGCGTTAATTGTCCTGCTTTgcgc                <  2:268145/140‑1 (MQ=255)
                                                                                                                    gcCTTTATCACCAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGTCCGGCAATGATGGCACTGCTCGCGGCGTTAATTGTCCTGCTTTgcgc                <  1:401216/140‑1 (MQ=255)
                                                                                                                               cAAAGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGTCCGGCAATGATGGCACTGCTCGCGGCGTTAATTGTCCTGCTTTGCGCATTTGTCGTTc     <  1:1534434/140‑1 (MQ=255)
                                                                                                                                 aaGTGCTTCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGTCCGGCAATGATGGCACTGCTCGCGGCGTTAATTGTCCTGCTTTGCGCATTTGTCGTTCAg   <  1:148947/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                     
AAAGCTATCGCCTGTCATGCGGATTTTACGCCTGCCAGTCACTGGTGCTGGTGTCTATTTTCGCCACTCTCTCGTGCCTGTTCGCCGCAGAGCAACTGCTGATCTGGTCCGCCAGCGCCTTTATCACCAAAGTGCTGCTGGTACCGTTAATCATGACTTACGCTGCACGAAATATTCCCCAGAACATCCCGGAAAAAGCGTTATTCGGTCCGGCAATGATGGCACTGCTCGCGGCGTTAATTGTCCTGCTTTGCGCATTTGTCGTTCAGC  >  NC_000913/2606335‑2606604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑