Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,838,693	C→A	P149Q (CCG→CAG)	yicL →	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,838,693	0	C	A	100.0%	289.2 / NA	81	P149Q (CCG→CAG)	yicL	putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (35/46);  total (35/46)

GGTGCTGCAATTCCTCTCACCGACGATTATCGTCGCCTGGTTCTCACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGGCTGGAGTATG  >  NC_000913/3838556‑3838826                                                                                                                                          |                                                                                                                                      ggTGCTGCAATTCCTCTCACCGACGATTATCGTCGCCTGGTTCTCACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGa                                                                                                                                     <  1:1189851/140‑1 (MQ=255)   tgctgcAATTCCTCTCACCGACGATTATCGTCGCCTGGTTCTCACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACg                                                                                                                                   <  2:18082/140‑1 (MQ=255)     ctgcAATTCCTCTCACCGACGATTATCGTCGCCTGGTTCTCACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTc                                                                                                                                 >  2:1484938/1‑140 (MQ=255)       gcAATTCCTCTCACCGACGATTATCGTCGCCTGGTTCTCACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCAt                                                                                                                               >  2:191172/1‑140 (MQ=255)          aTTCCTCTCACCGACGATTATCGTCGCCTGGTTCTCACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTAt                                                                                                                            <  1:487864/140‑1 (MQ=255)          aTTCCTCTCACCGACGATTATCGTCGCCTGGTTCTCACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTAt                                                                                                                            <  2:1542193/140‑1 (MQ=255)            tCCTCTCACCGACGATTATCGTCGCCTGGTTCTCACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCg                                                                                                                          >  1:2000856/1‑140 (MQ=255)             ccTCTCACCGACGATTATCGTCGCCTGGTTCTCACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCa                                                                                                                                <  1:1367572/133‑1 (MQ=255)             ccTCTCACCGACGATTATCGTCGCCTGGTTCTCACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCa                                                                                                                                >  2:1367572/1‑133 (MQ=255)               tctcACCGACGATTATCGTCGCCTGGTTCTCACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGAtc                                                                                                                       >  1:573709/1‑140 (MQ=255)               tctcACCGACGATTATCGTCGCCTGGTTCTCACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGAtc                                                                                                                       >  2:1082952/1‑140 (MQ=255)                ctcACCGACGATTATCGTCGCCTGGTTCTCACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGAtct                                                                                                                      >  1:1350098/1‑140 (MQ=255)                  cACCGACGATTATCGTCGCCTGGTTCTCACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGAtctct                                                                                                                    >  2:87754/1‑140 (MQ=255)                    ccGACGATTATCGTCGCCTGGTTCTCACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTcc                                                                                                                  >  2:1928255/1‑140 (MQ=255)                        cgaTTATCGTCGCCTGGTTCTCACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGcc                                                                                                              >  1:852098/1‑140 (MQ=255)                           ttATCGTCGCCTGGTTCTCACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCcgcg                                                                                                           >  1:1922760/1‑140 (MQ=255)                           ttATCGTCGCCTGGTTCTCACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCcgcg                                                                                                           <  1:101989/140‑1 (MQ=255)                           ttATCGTCGCCTGGTTCTCACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCcgcg                                                                                                           <  2:269841/140‑1 (MQ=255)                           ttATCGTCGCCTGGTTCTCACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCcgcg                                                                                                           <  1:1082952/140‑1 (MQ=255)                            tATCGTCGCCTGGTTCTCACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCAATCTCTCCTGCCGCgt                                                                                                          <  1:191172/140‑1 (MQ=255)                               cgtcgCCTGGTTCTCACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCgttgt                                                                                                       <  1:1484938/140‑1 (MQ=255)                                 tcgCCTGGTTCTCACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTc                                                                                                     >  2:1933662/1‑140 (MQ=255)                                  cgCCTGGTTCTCACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCt                                                                                                    >  1:1140502/1‑140 (MQ=255)                                  cgCCTGGTTCACACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCt                                                                                                    <  1:1753749/140‑1 (MQ=255)                                    ccTGGTTCTCACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTgg                                                                                                  >  1:1891954/1‑140 (MQ=255)                                            tcACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGc                                                                                          <  1:57007/140‑1 (MQ=255)                                            tcACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCACTGc                                                                                          >  2:857504/1‑140 (MQ=255)                                             cACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGcc                                                                                         <  1:878418/140‑1 (MQ=255)                                             cACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGcc                                                                                         <  2:1019327/140‑1 (MQ=255)                                             cACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGcc                                                                                         <  1:1928255/140‑1 (MQ=255)                                             cACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGcc                                                                                         <  1:1496035/140‑1 (MQ=255)                                                  gTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGc                                                                                    >  2:1234085/1‑140 (MQ=255)                                                   tGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCa                                                                                   >  2:211597/1‑140 (MQ=255)                                                   tGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCa                                                                                   >  1:1608499/1‑140 (MQ=255)                                                     cGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCa                                                                                              <  1:657451/127‑1 (MQ=255)                                                     cGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCa                                                                                              >  2:657451/1‑127 (MQ=255)                                                     cGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCAtt                                                                                 >  2:1864986/1‑140 (MQ=255)                                                     cGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCAtt                                                                                 >  1:251179/1‑140 (MQ=255)                                                     cGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCAtt                                                                                 >  1:1698897/1‑140 (MQ=255)                                                      gTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCAttt                                                                                >  1:1032039/1‑140 (MQ=255)                                                       tAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTtg                                                                               <  2:1891954/140‑1 (MQ=255)                                                          aTCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTtgctg                                                                            <  2:2000856/140‑1 (MQ=255)                                                            cgcgCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTtgctgct                                                                          <  1:981002/140‑1 (MQ=255)                                                            cgcgCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTtgctgct                                                                          <  1:1864986/140‑1 (MQ=255)                                                            cgcgCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTtgctgct                                                                          <  1:1282255/140‑1 (MQ=255)                                                            cgcgCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTtgctgct                                                                          <  2:852098/140‑1 (MQ=255)                                                            cgcgCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTtgctgct                                                                          <  1:87754/140‑1 (MQ=255)                                                            cgcgCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCACTGCCTCGGCATTtgctgct                                                                          <  1:857504/140‑1 (MQ=255)                                                             gcgcCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTtgctgctg                                                                         <  1:1933662/140‑1 (MQ=255)                                                             gcgcCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTtgctgctg                                                                         <  2:183853/140‑1 (MQ=255)                                                               gcCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCa                                                                       <  2:753574/140‑1 (MQ=255)                                                               gcCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCa                                                                       <  2:251179/140‑1 (MQ=255)                                                               gcCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCa                                                                       <  2:1350098/140‑1 (MQ=255)                                                               gcCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCa                                                                       >  2:244254/1‑140 (MQ=255)                                                                cccGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCAt                                                                      >  1:997574/1‑140 (MQ=255)                                                                      cATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCtata                                                                <  1:80919/140‑1 (MQ=255)                                                                      cATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCtata                                                                <  1:211597/140‑1 (MQ=255)                                                                        ttCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATacc                                                              <  1:1234085/140‑1 (MQ=255)                                                                        ttCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATacc                                                              <  2:997574/140‑1 (MQ=255)                                                                         tCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCT‑GGGCATTGCCTCGGCATTTGCTGCTGCATTGTATaccac                                                            <  2:672258/140‑1 (MQ=255)                                                                          cTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATaccac                                                            <  2:1140502/140‑1 (MQ=255)                                                                                   tGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTc                                                   >  1:556477/1‑140 (MQ=255)                                                                                   tGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTc                                                   >  1:195142/1‑140 (MQ=255)                                                                                        tATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGc                                              <  2:127971/140‑1 (MQ=255)                                                                                          ttttGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTa                                            >  2:1113738/1‑140 (MQ=255)                                                                                                cATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGcc                                      <  2:195142/140‑1 (MQ=255)                                                                                                    gCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCt                                  >  2:217751/1‑140 (MQ=255)                                                                                                    gCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCt                                  >  2:227255/1‑140 (MQ=255)                                                                                                    gCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCt                                  >  1:1758439/1‑140 (MQ=255)                                                                                                        gTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATgg                              <  1:594800/140‑1 (MQ=255)                                                                                                        gTCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATgg                              >  1:81841/1‑140 (MQ=255)                                                                                                         tCGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGc                             <  2:1758439/140‑1 (MQ=255)                                                                                                          cGGGACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCa                            <  2:1339154/140‑1 (MQ=255)                                                                                                           gggACTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCAc                           <  1:1113738/140‑1 (MQ=255)                                                                                                              aCTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGtt                        >  2:434524/1‑140 (MQ=255)                                                                                                               cTTTTTTATTGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTa                       >  1:267232/1‑140 (MQ=255)                                                                                                                ttttttATTGCTGACACACGGTAATCAGACGTCATTATTGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGGTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCGCGTTAc                      <  2:1094239/140‑1 (MQ=255)                                                                                                                       ttGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAgtcgt               <  2:267232/140‑1 (MQ=255)                                                                                                                       ttGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAgtcgt               <  2:1698897/140‑1 (MQ=255)                                                                                                                       ttGGTGACACACGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAgtcgt               <  2:1922760/140‑1 (MQ=255)                                                                                                                              cacacGGTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGGCTgg        <  2:1032039/140‑1 (MQ=255)                                                                                                                                    gTAATCAGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGGCTGGAGTATg  >  2:428518/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      GGTGCTGCAATTCCTCTCACCGACGATTATCGTCGCCTGGTTCTCACTGGTGCGTAAATCGCGCCCGGGCATTCTGGTTTTCTGCGCTATTTTGACATCGCTGGTCGGGACTTTTTTATTGGTGACACACGGTAATCCGACGTCATTATCGATCTCTCCTGCCGCGTTGTTCTGGGGCATTGCCTCGGCATTTGCTGCTGCATTCTATACCACCTATCCCTCAACGCTAATTGCCCGCTATGGCACGTTACCAGTCGTCGGCTGGAGTATG  >  NC_000913/3838556‑3838826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑