BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,261,710	C→A	S14R (AGC→AGA)	dusA →	tRNA‑dihydrouridine synthase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,261,710	0	C	A	100.0%	710.1 / NA	199	S14R (AGC→AGA)	dusA	tRNA‑dihydrouridine synthase A
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (109/90); total (109/90)

Only 100 of 200 total aligned reads displayed.

TATGGTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGCGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGAAATGGTGA  >  NC_000913/4261575‑4261846

tATGGTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCaa                                                                                                                                        >  2:1480623/1‑140 (MQ=255)

       taGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGa                                                                                                                                 >  1:517997/1‑140 (MQ=255)

        aGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGaa                                                                                                                                >  1:68084/1‑140 (MQ=255)

         gTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGaaa                                                                                                                               >  1:1835982/1‑140 (MQ=255)

          tACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGaaaa                                                                                                                              >  2:1561194/1‑140 (MQ=255)

          tACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGaaaa                                                                                                                              >  2:1368587/1‑140 (MQ=255)

            ccccGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGaaaaaa                                                                                                                            >  2:603468/1‑140 (MQ=255)

              ccGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACt                                                                                                                          >  1:1705054/1‑140 (MQ=255)

               cGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTg                                                                                                                         >  2:711131/1‑140 (MQ=255)

               cGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTg                                                                                                                         >  2:1196929/1‑140 (MQ=255)

                 cTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGAc                                                                                                                       <  2:1998872/140‑1 (MQ=255)

                 cTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGAc                                                                                                                       <  1:1014148/140‑1 (MQ=255)

                  tATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACg                                                                                                                      <  2:484236/140‑1 (MQ=255)

                    ttGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGAc                                                                                                                       >  2:361369/1‑137 (MQ=255)

                    ttGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGtt                                                                                                                    >  2:1421166/1‑140 (MQ=255)

                    ttGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGtt                                                                                                                    >  1:1104187/1‑140 (MQ=255)

                       aGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGaa                                                                                                                                >  1:833518/1‑125 (MQ=255)

                       aGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGaa                                                                                                                                <  2:833518/125‑1 (MQ=255)

                           tcctGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTg                                                                                                                         >  1:1917633/1‑128 (MQ=255)

                           tcctGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGa                                                                                                           <  1:33977/140‑1 (MQ=255)

                                aaTAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGt                                                                                                      <  1:301768/140‑1 (MQ=255)

                                aaTAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGt                                                                                                      <  2:1705054/140‑1 (MQ=255)

                                       aTGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACACTGGAGTGGTCGGtt                                                                                                 >  1:555594/1‑140 (MQ=255)

                                       aTGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTa                                                                                               >  2:1149631/1‑140 (MQ=255)

                                       aTGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTa                                                                                               >  2:506407/1‑140 (MQ=255)

                                        tGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAg                                                                                              >  2:1359826/1‑140 (MQ=255)

                                          cTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCg                                                                                            <  2:1905313/140‑1 (MQ=255)

                                          cTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCg                                                                                            >  1:1991524/1‑140 (MQ=255)

                                               taacataacCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCa                                                                                       >  1:1080213/1‑140 (MQ=255)

                                               taacataacCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCa                                                                                       >  1:1797041/1‑140 (MQ=255)

                                                  cataacCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCa                                                                                    >  2:1822763/1‑140 (MQ=255)

                                                       ccccATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCt                                                                               >  1:1150217/1‑140 (MQ=255)

                                                       ccccATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCt                                                                               >  1:1222420/1‑140 (MQ=255)

                                                       ccccATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCt                                                                               >  1:52646/1‑140 (MQ=255)

                                                       ccccATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCt                                                                               >  2:1233889/1‑140 (MQ=255)

                                                       ccccATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCt                                                                               >  2:1170146/1‑140 (MQ=255)

                                                       ccccATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAACCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCt                                                                               >  2:432469/1‑140 (MQ=255)

                                                         ccATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCGATGCTCg                                                                             <  1:1369858/140‑1 (MQ=255)

                                                          cATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGa                                                                            >  1:446091/1‑140 (MQ=255)

                                                            tGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACt                                                                          >  1:912507/1‑140 (MQ=255)

                                                            tGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACt                                                                          >  1:595730/1‑140 (MQ=255)

                                                            tGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACt                                                                          >  2:1325624/1‑140 (MQ=255)

                                                            tGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACt                                                                          >  1:1135754/1‑140 (MQ=255)

                                                             gATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTg                                                                         >  1:391404/1‑140 (MQ=255)

                                                             gATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTg                                                                         <  2:68084/140‑1 (MQ=255)

                                                              atatatCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTgg                                                                        <  2:1135754/140‑1 (MQ=255)

                                                                atatCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTggac                                                                      <  2:1080213/140‑1 (MQ=255)

                                                                 tatCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTgga                                                                       >  1:1366183/1‑138 (MQ=255)

                                                                  atCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTggacgg                                                                    >  2:879164/1‑140 (MQ=255)

                                                                  atCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTggacgg                                                                    <  2:198419/140‑1 (MQ=255)

                                                                  atCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTggacgg                                                                    >  1:24876/1‑140 (MQ=255)

                                                                  atCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTggacgg                                                                    >  1:376293/1‑140 (MQ=255)

                                                                     gATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGgaca                                                                 >  1:782397/1‑140 (MQ=255)

                                                                      aTAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGgacag                                                                >  2:1618764/1‑140 (MQ=255)

                                                                      aTAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGgacag                                                                >  2:1024381/1‑140 (MQ=255)

                                                                       tAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTggacg                                                                     >  2:704881/1‑134 (MQ=255)

                                                                       tAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTggacg                                                                     <  1:704881/134‑1 (MQ=255)

                                                                       tAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGgacaga                                                               >  1:857844/1‑140 (MQ=255)

                                                                         aaaTAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGgacagaca                                                             <  2:1104187/140‑1 (MQ=255)

                                                                          aataatCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTgg                                                                        <  1:133105/128‑1 (MQ=255)

                                                                          aataatCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACAt                                                            <  2:283332/140‑1 (MQ=255)

                                                                           ataatCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACAtt                                                           <  1:273261/140‑1 (MQ=255)

                                                                              atCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGcc                                                        <  2:24876/140‑1 (MQ=255)

                                                                                    aCATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTAtt                                                  >  2:1643687/1‑140 (MQ=255)

                                                                                     cATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGgaca                                                                 >  1:836673/1‑124 (MQ=255)

                                                                                     cATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGgaca                                                                 <  2:836673/124‑1 (MQ=255)

                                                                                     cATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTAttt                                                 >  2:173467/1‑140 (MQ=255)

                                                                                          gAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGc                                            <  2:391404/140‑1 (MQ=255)

                                                                                             aaTGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGTAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTc                                         >  1:578607/1‑140 (MQ=255)

                                                                                               tGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTg                                       >  1:502776/1‑140 (MQ=255)

                                                                                                  aCGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCtt                                    <  2:595730/140‑1 (MQ=255)

                                                                                                  aCGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCtt                                    <  2:52646/140‑1 (MQ=255)

                                                                                                      tAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTccc                                >  1:946524/1‑140 (MQ=255)

                                                                                                       aaTTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCg                               <  1:1024381/140‑1 (MQ=255)

                                                                                                         ttCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCa                             <  1:879164/140‑1 (MQ=255)

                                                                                                            tGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACTGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATa                          <  2:517997/140‑1 (MQ=255)

                                                                                                            tGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATa                          <  1:1243347/140‑1 (MQ=255)

                                                                                                            tGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATa                          <  1:603468/140‑1 (MQ=255)

                                                                                                              aaaTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGAc                                                                                                                       >  1:427418/1‑47 (MQ=255)

                                                                                                              aaaTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACg                        <  2:857844/140‑1 (MQ=255)

                                                                                                              aaaTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACg                        <  2:1171792/140‑1 (MQ=255)

                                                                                                              aaaTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACg                        <  1:1561194/140‑1 (MQ=255)

                                                                                                                aTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACg                        <  1:470251/138‑1 (MQ=255)

                                                                                                                aTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACg                        >  2:470251/1‑138 (MQ=255)

                                                                                                                aTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGtt                      <  2:456943/140‑1 (MQ=255)

                                                                                                                 tGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTg                     <  1:12040/140‑1 (MQ=255)

                                                                                                                 tGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTg                     <  1:1857908/140‑1 (MQ=255)

                                                                                                                   cAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTcc                                 >  2:1060024/1‑126 (MQ=255)

                                                                                                                   cAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATa                          >  1:1586123/1‑133 (MQ=255)

                                                                                                                   cAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCt                   <  2:302020/140‑1 (MQ=255)

                                                                                                                   cAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCt                   >  2:745672/1‑140 (MQ=255)

                                                                                                                   cAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCt                   <  1:1064818/140‑1 (MQ=255)

                                                                                                                      aaaaTCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGta                <  2:946524/140‑1 (MQ=255)

                                                                                                                      aaaaTCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGta                <  1:745672/140‑1 (MQ=255)

                                                                                                                      aaaaTCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGCTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGta                <  1:1190220/140‑1 (MQ=255)

                                                                                                                        aaTCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGtata              <  2:1220698/140‑1 (MQ=255)

                                                                                                                           cAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCg           <  2:1934579/140‑1 (MQ=255)

                                                                                                                             accaaaccaGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGaa         <  2:1847363/140‑1 (MQ=255)

                                                                                                                                  accaGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGAAATGGt    <  1:1466530/140‑1 (MQ=255)

                                                                                                                                  accaGAGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGAAATGGt    <  1:1618764/140‑1 (MQ=255)

TATGGTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGCGCAATGCCTGAAAAAACTGACGTT‑‑CACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGAAATGGTGA  >  NC_000913/4261575‑4261846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`