Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,184,920	C→A	A128S (GCT→TCT)	thiM ←	hydroxyethylthiazole kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,184,920	0	C	A	100.0%	346.3 / NA	96	A128S (GCT→TCT)	thiM	hydroxyethylthiazole kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (44/52);  total (44/52)

CTCGCCAGTGACCACGACGATTGCGCCAGTTTCCCGTGCCAGTGTTTGTGCAGCGGGTATCGCGTTAGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGCATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAG  >  NC_000913/2184783‑2185051                                                                                                                                          |                                                                                                                                    ctcGCCAGTGACCACGACGATTGCGCCAGTTTCCCGTGCCAGTGTTTGTGCAGCGGGTATCGCGTTAGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAAt                                                                                                                                   >  2:277770/1‑140 (MQ=255) ctcGCCAGTGACCACGACGATTGCGCCAGTTTCCCGTGCCAGTGTTTGTGCAGCGGGTATCGCGTTAGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAAt                                                                                                                                   >  1:1187761/1‑140 (MQ=255) ctcGCCAGTGACCACGACGATTGCGCCAGTTTCCCGTGCCAGTGTTTGTGCAGCGGGTATCGCGTTAGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAAt                                                                                                                                   >  1:1439845/1‑140 (MQ=255) ctcGCCAGTGACCACGACGATTGCGCCAGTTTCCCGTGCCAGTGTTTGTGCAGCGGGTATCGCGTTAGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAAt                                                                                                                                   >  2:1097694/1‑140 (MQ=255)  tcGCCAGTGACCACGACGATTGCGCCAGTTTCCCGTGCCAGTGTTTGTGCAGCGGGTATCGCGTTAGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAAtt                                                                                                                                  >  1:1242407/1‑140 (MQ=255)  tcGCCAGTGACCACGACGATTGCGCCAGTTTCCCGTGCCAGTGTTTGTGCAGCGGGTATCGCGTTAGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAAtt                                                                                                                                  >  2:638550/1‑140 (MQ=255)   cGCCAGTGACCACGACGATTGCGCCAGTTTCCCGTGCCAGTGTTTGTGCAGCGGGTATCGCGTTAGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTa                                                                                                                                 <  1:576054/140‑1 (MQ=255)    gCCAGTGACCACGACGATTGCGCCAGTTTCCCGTGCCAGTGTTTGTGCAGCGGGTATCGCGTTAGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTAc                                                                                                                                <  1:1097694/140‑1 (MQ=255)     ccAGTGACCACGACGATTGCGCCAGTTTCCCGTGCCAGTGTTTGTGCAGCGGGTATCGCGTTAGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTAcc                                                                                                                               >  2:591923/1‑140 (MQ=255)      cAGTGACCACGACGATTGCGCCAGTTTCCCGTGCCAGTGTTTGTGCAGCGGGTATCGTGTTAGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCa                                                                                                                              <  1:1484432/140‑1 (MQ=255)          gACCACGACGATTGCGCCAGTTTCCCGTGCCAGTGTTTGTGCAGCGGGTATCGCGTTAGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTa                                                                                                                          >  2:1373509/1‑140 (MQ=255)          gACCACGACGATTGCGCCAGTTTCCCGTGCCAGTGTTTGTGCAGCGGGTATCGCGTTAGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATGACCACGTa                                                                                                                          >  1:1401926/1‑140 (MQ=255)               cgacgaTTGCGCCAGTTTCCCGTGCCAGTGTTTGTGCAGCGGGTATCGCGTTAGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCt                                                                                                                     <  1:591923/140‑1 (MQ=255)                 acgaTTGCGCCAGTTTCCCGTGCCAGTGTTTGTGCAGCGGGTATCGCGTTAGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGc                                                                                                                   <  2:1187761/140‑1 (MQ=255)                  cgaTTGCGCCAGTTTCCCGTGCCAGTGTTTGTGCAGCGGGTATCGCGTTAGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGcc                                                                                                                  <  1:978193/140‑1 (MQ=255)                   gaTTGCGCCAGTTTCCCGTGCCAGTGTTTGTGCAGCGGGTATCGCGTTAGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCg                                                                                                                 >  2:133060/1‑140 (MQ=255)                        cgcCAGTTTCCCGTGCCAGTGTTTGTGCAGCGGGTATCGCGTTAGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTa                                                                                                            <  2:1003097/140‑1 (MQ=255)                        cgcCAGTTTCCCGTGCCAGTGTTTGTGCAGCGGGTATCGCGTTAGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTa                                                                                                            >  2:516831/1‑140 (MQ=255)                         gcCAGTTTCCCGTGCCAGTGTTTGTGCAGCGGGTATCGCGTTAGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTaa                                                                                                           <  2:1479835/140‑1 (MQ=255)                          ccAGTTTCCCGTGCCAGTGTTTGTGCAGCGGGTATCGCGTTAGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTaaa                                                                                                          >  1:863112/1‑140 (MQ=255)                             gTTTCCCGTGCCAGTGTTTGTGCAGCGGGTATCGCGTTAGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGa                                                                                                       >  2:403219/1‑140 (MQ=255)                                tCCCGTGCCAGTGTTTGTGCAGCGGGTATCGCGTTAGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATaa                                                                                                    <  2:863112/140‑1 (MQ=255)                                tCCCGTGCCAGTGTTTGTGCAGCGGGTATCGCGTTAGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATaa                                                                                                    <  2:1437542/140‑1 (MQ=255)                                tCCCGTGCCAGTGTTTGTGCAGCGGGTATCGCGTTAGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCAGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATaa                                                                                                    >  2:1283884/1‑140 (MQ=255)                                       ccAGTGTTTGTGCAGCGGGTATCGCGTTAGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCa                                                                                             >  1:419036/1‑140 (MQ=255)                                       ccAGTGTTTGTGCAGCGGGTATCGCGTTAGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCa                                                                                             >  1:585691/1‑140 (MQ=255)                                        cAGTGTTTGTGCAGCGGGTATCGCGTTAGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCAt                                                                                            <  2:1433696/140‑1 (MQ=255)                                          gtgtTTGTGCAGCGGGTATCGCGTTAGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGa                                                                                          >  2:1120764/1‑140 (MQ=255)                                              ttGTGCAGCGGGTATCGCGTTAGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACaaa                                                                                      <  2:1520569/140‑1 (MQ=255)                                                 tgCAGCGGGTATCGCGTTAGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAAt                                                                                   >  2:1511382/1‑140 (MQ=255)                                                  gCAGCGGGTAGCGCGTTAGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATg                                                                                  <  1:1120764/140‑1 (MQ=255)                                                     gCGGGTATCGCGTTAGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATggcg                                                                               >  2:1064230/1‑140 (MQ=255)                                                     gCGGGTATCGCGTTAGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATggcg                                                                               >  1:547763/1‑140 (MQ=255)                                                     gCGGGTATCGCGTTAGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATggc                                                                                >  1:436292/1‑139 (MQ=255)                                                     gCGGGTATCGCGTTAGCTGCGGCGTCAGGGGTATCCCCTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATggc                                                                                <  2:436292/139‑1 (MQ=255)                                                      cGGGTATCGCGTTAGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATggcgg                                                                              >  2:302196/1‑140 (MQ=255)                                                      cGGGTATCGCGTTAGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATggcgg                                                                              >  1:196227/1‑140 (MQ=255)                                                      cGGGTATCGCGTTAGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATggcgg                                                                              >  1:140245/1‑140 (MQ=255)                                                        ggTATCGCGTTAGCTGCGGCGTCAGTGGTATCCACTCCCTGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGgcggcg                                                                            <  2:1439845/140‑1 (MQ=255)                                                             cgcgTTAGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAAt                                                                       <  2:2680/140‑1 (MQ=255)                                                             cgcgTTAGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCAGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAAt                                                                       <  1:1283884/140‑1 (MQ=255)                                                                  tAGCTGCGGCGTCAGTGGTATCCCCTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAgc                                                                  <  2:626789/140‑1 (MQ=255)                                                                  tAGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAgc                                                                  >  2:548094/1‑140 (MQ=255)                                                                   aGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAgcg                                                                 <  1:951184/140‑1 (MQ=255)                                                                      tGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGcac                                                              <  2:1242407/140‑1 (MQ=255)                                                                        cggcgTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGca                                                               >  2:100725/1‑137 (MQ=255)                                                                        cggcgTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGca                                                               <  1:100725/137‑1 (MQ=255)                                                                          gcgTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGcacccac                                                          <  2:140245/140‑1 (MQ=255)                                                                          gcgTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGcacccac                                                          <  2:390429/140‑1 (MQ=255)                                                                          gcgTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGcacccac                                                          <  2:585691/140‑1 (MQ=255)                                                                          gcgTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGcacccac                                                          <  2:1200701/140‑1 (MQ=255)                                                                          gcgTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGcacccac                                                          <  1:1064230/140‑1 (MQ=255)                                                                            gTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCg                                                        <  1:1373509/140‑1 (MQ=255)                                                                                gTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTAc                                                    <  1:750050/140‑1 (MQ=255)                                                                                gTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTAc                                                    <  1:516831/140‑1 (MQ=255)                                                                                  ggTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTg                                                  <  1:1055654/140‑1 (MQ=255)                                                                                   gTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTgg                                                 <  1:403219/140‑1 (MQ=255)                                                                                     aTCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGAt                                               >  2:1395199/1‑140 (MQ=255)                                                                                       ccccTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCa                                             <  2:236131/137‑1 (MQ=255)                                                                                       ccACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTAc                                                    <  1:481728/133‑1 (MQ=255)                                                                                       ccACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTAc                                                    >  2:481728/1‑133 (MQ=255)                                                                                       ccACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCa                                             <  2:1473786/140‑1 (MQ=255)                                                                                       ccACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCa                                             <  2:937342/140‑1 (MQ=255)                                                                                        cACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCaa                                            <  1:881088/140‑1 (MQ=255)                                                                                         ccTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAg                                           <  1:1260024/139‑1 (MQ=255)                                                                                          cTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGc                                          <  1:133060/140‑1 (MQ=255)                                                                                           tCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCg                                         >  1:420846/1‑140 (MQ=255)                                                                                             cccGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTc                                       >  1:392250/1‑140 (MQ=255)                                                                                              ccGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCa                                             <  1:848689/133‑1 (MQ=255)                                                                                              ccGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCa                                             >  2:848689/1‑133 (MQ=255)                                                                                              ccGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTcc                                      >  2:1302088/1‑140 (MQ=255)                                                                                               cGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCa                                     <  1:277770/140‑1 (MQ=255)                                                                                                gTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTa                                                     <  1:689270/123‑1 (MQ=255)                                                                                                gTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTa                                                     >  2:689270/1‑123 (MQ=255)                                                                                                 tCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAgg                                   >  1:509552/1‑140 (MQ=255)                                                                                                  ccGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAggg                                  >  2:944422/1‑140 (MQ=255)                                                                                                   cGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCa                                             <  2:303311/128‑1 (MQ=255)                                                                                                   cGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCa                                             >  1:303311/1‑128 (MQ=255)                                                                                                    gCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGgtg                                <  2:196227/140‑1 (MQ=255)                                                                                                         tAGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTga                           <  1:548094/140‑1 (MQ=255)                                                                                                          aGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTgag                          <  1:1302088/140‑1 (MQ=255)                                                                                                          aGCAATGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTgag                          <  1:1511382/140‑1 (MQ=255)                                                                                                               tGCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCtt                     <  1:638550/140‑1 (MQ=255)                                                                                                                gCCAGCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCttt                    >  1:652986/1‑140 (MQ=255)                                                                                                                    gCTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTttgc                >  2:12561/1‑140 (MQ=255)                                                                                                                     cTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTttgct               <  1:302196/140‑1 (MQ=255)                                                                                                                     cTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTttgct               <  1:12561/140‑1 (MQ=255)                                                                                                                     cTAATGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTttgct               >  2:989307/1‑140 (MQ=255)                                                                                                                         tGCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTtgcttgct           >  2:1274151/1‑140 (MQ=255)                                                                                                                          gCCATGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTc          >  1:535724/1‑140 (MQ=255)                                                                                                                              tGATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACg      <  1:889319/140‑1 (MQ=255)                                                                                                                               gATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACgg     >  2:827208/1‑140 (MQ=255)                                                                                                                               gATTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACgg     >  2:1252814/1‑140 (MQ=255)                                                                                                                                aTTTCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGgc    >  2:546311/1‑140 (MQ=255)                                                                                                                                  ttCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCCCCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGgaag  <  2:547763/140‑1 (MQ=255)                                                                                                                                  ttCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGgcag  <  2:509552/140‑1 (MQ=255)                                                                                                                                  ttCCGAAGAATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGgcag  <  2:886964/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                    CTCGCCAGTGACCACGACGATTGCGCCAGTTTCCCGTGCCAGTGTTTGTGCAGCGGGTATCGCGTTAGCTGCGGCGTCAGTGGTATCCACTCCCCGTCCGCCATTAGCAATGCCAGCTAATGCCATGATTTCCGAAGCATTACCACGTATCGCTGCCGGTTTAAAAGATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAG  >  NC_000913/2184783‑2185051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑