Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,270,816 C→A S53R (AGC→AGA)  yjbR → PF04237 family protein YjbR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,270,8160CA100.0% 349.8 / NA 101S53R (AGC→AGA) yjbRPF04237 family protein YjbR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (49/52);  total (49/52)

Only 100 of 103 total aligned reads displayed.

AATATTGCATGGCAAAACCAGGCGCAGAACAGAGCGTGCATAATGACTGGAAAGCGACGCAGATCAAAGTGGAAGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGCCCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACGGTTCGCTGCCAGATTCGCAAATCTATTATCTGGTGGATGC  >  NC_000913/4270680‑4270950
                                                                                                                                        |                                                                                                                                      
aaTATTGCATGGCAAAACCAGGCGCAGAACAGAGCGTGCATAATGACTGGAAAGCGACGCAGATCAAAGTGGAAGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCg                                                                                                                                     <  1:1273403/140‑1 (MQ=255)
     tGCATGGCAAAACCAGGCGCATAACAGAGCGTGCATAATGACTGGAAAGCGACGCAGATCAAAGTGGAAGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCt                                                                                                                                >  2:402848/1‑140 (MQ=255)
       cATGGCAAAACCAGGCGCAGAACAGAGCGTGCATAATGACTGGAAAGCGACGCAGATCAAAGTGGAAGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTgg                                                                                                                              >  2:452424/1‑140 (MQ=255)
         tGGCAAAACCAGGCGCAGAACAGAGCGTGCATAATGACTGGAAAGCGACGCAGATCAAAGTGGAAGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCg                                                                                                                            >  1:904247/1‑140 (MQ=255)
          ggCAAAACCAGGCGCAGAACAGAGCGTGCATAATGACTGGAAAGCGACGCAGATCAAAGTGGAAGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCgg                                                                                                                           >  2:805878/1‑140 (MQ=255)
          ggCAAAACCAGGCGCAGAACAGAGCGTGCATAATGACTGGAAAGCGACGCAGATCAAAGTGGAAGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCgg                                                                                                                           >  1:389859/1‑140 (MQ=255)
           gCAAAACCAGGCGCAGAACAGAGCGTGCATAATGACTGGAAAGCGACGCAGATCAAAGTGGAAGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGa                                                                                                                          <  1:151385/140‑1 (MQ=255)
            cAAAACCAGGCGCAGAACAGAGCGTGCATAATGACTGGAAAGCGACGCAGATCAAAGTGGAAGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAg                                                                                                                         >  1:739025/1‑140 (MQ=255)
            cAAAACCAGGCGCAGAACAGAGCGTGCATAATGACTGGAAAGCGACGCAGATCAAAGTGGAAGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAg                                                                                                                         >  2:174360/1‑140 (MQ=255)
                 ccAGGCGCAGAACAGAGCGTGCATAATGACTGGAAAGCGACGCAGATCAAAGTGGAAGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAgctgct                                                                                                                    <  2:904247/140‑1 (MQ=255)
                   aGGCGCAGAACAGAGCGTGCATAATGACTGGAAAGCGACGCAGATCAAAGTGGAAGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTAc                                                                                                                  <  1:901043/140‑1 (MQ=255)
                      cgcAGAACAGAGCGTGCATAATGACTGGACAGCGACGCAGATCAAAGTGGAAGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTc                                                                                                               >  2:1496093/1‑140 (MQ=255)
                        cAGAACAGAGCGTGCATAATGACTGGAAAGCGACGCAGATCAAAGTGGAAGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTcag                                                                                                             <  1:805878/140‑1 (MQ=255)
                         aGAACAGAGCGTGCATAATGACTGGAAAGCGACGCAGATCAAAGTGGAAGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTcagc                                                                                                            >  2:1015490/1‑140 (MQ=255)
                           aaCAGAGCGTGCATAATGACTGGAAAGCGACGCAGATCAAAGTGGAAGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTcagcag                                                                                                          <  1:1015490/140‑1 (MQ=255)
                                agCGTGCATAATGACTGGAAAGCGACGCAGATCAAAGTGGAAGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAg                                                                                                     <  2:565451/140‑1 (MQ=255)
                                 gCGTGCATAATGACTGGAAAGCGACGCAGATCAAAGTGGAAGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGc                                                                                                    >  2:1213021/1‑140 (MQ=255)
                                  cGTGCATAATGACTGGAAAGCGACGCAGATCAAAGTGGAAGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCg                                                                                                   <  1:174360/140‑1 (MQ=255)
                                   gTGCATAATGACTGGAAAGCGACGCAGATCAAAGTGGAAGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGa                                                                                                  >  1:85202/1‑140 (MQ=255)
                                      cATAATGACTGGAAAGCGACGCAGATCAAAGTGGAAGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGAtgt                                                                                               >  2:1241559/1‑140 (MQ=255)
                                          aTGACTGGAAAGCGACGCAGATCAAAGTGGAAGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGt                                                                                           <  1:1315956/140‑1 (MQ=255)
                                                 gAAAGCGACGCAGATCAAAGTGGAAGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAgcc                                                                                    <  1:781772/140‑1 (MQ=255)
                                                 gAAAGCGACGCAGATCAAAGTGGAAGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAgcc                                                                                    >  1:444016/1‑140 (MQ=255)
                                                 gAAAGCGACGCAGATCAAAGTGGAAGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAgcc                                                                                    >  1:556770/1‑140 (MQ=255)
                                                   aaGCGACGCAGATCAAAGTGGAAGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAgccgc                                                                                  <  2:556770/140‑1 (MQ=255)
                                                   aaGCGACGCAGATCAAAGTGGAAGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAgccgc                                                                                  <  2:1123840/140‑1 (MQ=255)
                                                        aCGCAGATCAAAGTGGAAGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCt                                                                             >  1:621930/1‑140 (MQ=255)
                                                         cGCAGATCAAAGTGGAAGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTg                                                                            >  2:525107/1‑140 (MQ=255)
                                                         cGCAGATCAAAGTGGAAGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTg                                                                            <  1:1433241/140‑1 (MQ=255)
                                                             gATCAAAGTGGAAGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATa                                                                        <  2:444016/140‑1 (MQ=255)
                                                               tCAAAGTGGAAGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATaaa                                                                      <  2:389859/140‑1 (MQ=255)
                                                                cAAAGTGGAAGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAg                                                                     <  1:1231641/140‑1 (MQ=255)
                                                                  aaGTGGAAGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAgcg                                                                   <  1:402848/140‑1 (MQ=255)
                                                                  aaGTGGAAGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAgcg                                                                   <  2:525765/140‑1 (MQ=255)
                                                                  aaGTGGAAGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAgcg                                                                   <  2:960291/140‑1 (MQ=255)
                                                                      ggAAGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACt                                                               <  2:341804/140‑1 (MQ=255)
                                                                        aaGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTgg                                                             >  2:796052/1‑140 (MQ=255)
                                                                        aaGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTgg                                                             >  2:702449/1‑140 (MQ=255)
                                                                        aaGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTgg                                                             >  1:1299799/1‑140 (MQ=255)
                                                                        aaGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTgg                                                             >  2:909432/1‑140 (MQ=255)
                                                                          gATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAg                                                           <  2:1299799/140‑1 (MQ=255)
                                                                            tGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGTTGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCa                                                         <  1:1496093/140‑1 (MQ=255)
                                                                            tGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCa                                                         <  2:739025/140‑1 (MQ=255)
                                                                            tGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCa                                                         >  2:850415/1‑140 (MQ=255)
                                                                             gTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCAc                                                        >  1:454326/1‑140 (MQ=255)
                                                                               aCTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCg                                                      <  1:850415/140‑1 (MQ=255)
                                                                               aCTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCg                                                      <  2:455231/140‑1 (MQ=255)
                                                                               aCTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCg                                                      >  1:235570/1‑140 (MQ=255)
                                                                               aCTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCg                                                      >  1:1031906/1‑140 (MQ=255)
                                                                               aCTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCg                                                      >  2:58351/1‑140 (MQ=255)
                                                                                   tttGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTa                                                  >  1:1020007/1‑140 (MQ=255)
                                                                                   tttGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTa                                                  >  1:1436878/1‑140 (MQ=255)
                                                                                     tGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCa                                                         >  1:863458/1‑131 (MQ=255)
                                                                                     tGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCa                                                         <  2:863458/131‑1 (MQ=255)
                                                                                     tGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTAtc                                                >  1:754974/1‑140 (MQ=255)
                                                                                     tGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTAtc                                                >  1:334916/1‑140 (MQ=255)
                                                                                      gCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTAtct                                               >  2:1244922/1‑140 (MQ=255)
                                                                                       cGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCgtg                                                    >  1:1307800/1‑134 (MQ=255)
                                                                                       cGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCgtg                                                    <  2:1307800/134‑1 (MQ=255)
                                                                                           ggTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGc                                                          <  2:374085/124‑1 (MQ=255)
                                                                                           ggTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGc                                                          >  1:374085/1‑124 (MQ=255)
                                                                                           ggTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACg                                          >  2:808347/1‑140 (MQ=255)
                                                                                           ggTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACg                                          >  1:22070/1‑140 (MQ=255)
                                                                                             tGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACGGt                                        >  2:887313/1‑140 (MQ=255)
                                                                                             tGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACGGt                                        <  1:1241559/140‑1 (MQ=255)
                                                                                             tGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACGGt                                        >  1:1406060/1‑140 (MQ=255)
                                                                                             tGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACGGt                                        >  2:529376/1‑140 (MQ=255)
                                                                                             agcaagaagTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGCCCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACGGt                                        >  2:590658/4‑140 (MQ=255)
                                                                                              gAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACGGtt                                       <  2:754974/140‑1 (MQ=255)
                                                                                               aaaGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCATCGTGTATCTCGACGGTTc                                      <  2:95691/140‑1 (MQ=255)
                                                                                                    agTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACGGtt                                       <  1:217370/134‑1 (MQ=255)
                                                                                                      tAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACGGtt                                       <  2:165118/132‑1 (MQ=255)
                                                                                                      tAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACGGtt                                       >  1:165118/1‑132 (MQ=255)
                                                                                                      tAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACGGTTCGCTGCCa                               >  1:788062/1‑140 (MQ=255)
                                                                                                      tAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACGGTTCGCTGCCa                               >  1:923946/1‑140 (MQ=255)
                                                                                                      tAGAAAATCGCCCAGCTGTTTCACTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACGGTTCGCTGCCa                               >  1:344393/1‑140 (MQ=255)
                                                                                                           aaTCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACGGTTCGCTGCCAGATTc                          >  1:583639/1‑140 (MQ=255)
                                                                                                             tCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACGGTTCGCTGCCAGATTCGc                        <  1:529376/140‑1 (MQ=255)
                                                                                                             tCGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACGGTTCGCTGCCAGATTCGc                        <  2:541019/140‑1 (MQ=255)
                                                                                                              cGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACGGTTCGCTGCCAGATTCGCa                       <  2:1020007/140‑1 (MQ=255)
                                                                                                              cGCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACGGTTCGCTGCCAGATTCGCa                       <  2:1436878/140‑1 (MQ=255)
                                                                                                              cGCCCAGCTGTTTCACTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACGGTTCGCTGCCAGATTCGCa                       <  2:344393/140‑1 (MQ=255)
                                                                                                               gCCCAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACGGTTCGCTGCCAGATTCGCaa                      <  1:58351/140‑1 (MQ=255)
                                                                                                                cccAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACGGTTCGCTGCCAGATTCGCaaa                     >  1:243897/1‑140 (MQ=255)
                                                                                                                cccAGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACGGTTCGCTGCCAGATTCGCaaa                     >  1:155929/1‑140 (MQ=255)
                                                                                                                   aGCTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACGGTTCGCTGCCAGATTCGCAAATCt                  <  2:621930/140‑1 (MQ=255)
                                                                                                                     cTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACGGTTCGCTGCCAGATTCGCAAAt                    <  2:1388750/136‑1 (MQ=255)
                                                                                                                     cTGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACGGTTCGCTGCCAGATTCGCAAAt                    >  1:1388750/1‑136 (MQ=255)
                                                                                                                      tGTTTCGCTGAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACGGTTCGCTGCCAGATTCGCAAATCtatt               <  1:909432/140‑1 (MQ=255)
                                                                                                                               gAAAACCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACGGTTCGCTGCCAGATTCGCAAATCTATTATCtggtgg      >  2:801956/1‑140 (MQ=255)
                                                                                                                                aaaaCCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACGGTTCGCTGCCAGATTCGCAAATCTATTATCTGGTGGa     <  1:1213021/140‑1 (MQ=255)
                                                                                                                                aaaaCCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACGGTTCGCTGCCAGATTCGCAAATCTATTATCTGGTGGa     <  2:1031906/140‑1 (MQ=255)
                                                                                                                                aaaaCCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACGGTTCGCTGCCAGATTCGCAAATCTATTATCTGGTGGa     >  2:1209572/1‑140 (MQ=255)
                                                                                                                                aaaaCCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACGGTTCGCTGCCAGATTCGCAAATCTATTATCTGGTGGa     <  1:808347/140‑1 (MQ=255)
                                                                                                                                aaaaCCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACGGTTCGCTGCCAGATTCGCAAATCTATTATCTGGTGGa     <  2:155929/140‑1 (MQ=255)
                                                                                                                                aaaaCCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACGGTTCGCTGCCAGATTCGCAAATCTATTATCTGGTGGa     <  1:702449/140‑1 (MQ=255)
                                                                                                                                aaaaCCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACGGTTCGCTGCCAGATTCGCAAATCTATTATCTGGTGGa     <  2:235570/140‑1 (MQ=255)
                                                                                                                                aaaaCCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACGGTTCGCTGCCAGATTCGCAAATCTATTATCTGGTGGa     <  2:243897/140‑1 (MQ=255)
                                                                                                                                aaaaCCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACGGTTCGCTGCCAGATTCGCAAATCTATTATCTGGTGGa     <  1:212868/140‑1 (MQ=255)
                                                                                                                                   aCCAGACCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACGGTTCGCTGCCAGATTCGCAAATCTATTATCTGGTGGATGc  <  1:423408/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                      
AATATTGCATGGCAAAACCAGGCGCAGAACAGAGCGTGCATAATGACTGGAAAGCGACGCAGATCAAAGTGGAAGATGTACTGTTTGCGATGGTGAAAGAAGTAGAAAATCGCCCAGCTGTTTCGCTGAAAACCAGCCCGGAGCTGGCGGAGCTGCTACGTCAGCAGCACAGCGATGTGCGTCCAAGCCGCCATCTGAATAAAGCGCACTGGAGCACCGTGTATCTCGACGGTTCGCTGCCAGATTCGCAAATCTATTATCTGGTGGATGC  >  NC_000913/4270680‑4270950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑