| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,495,519 | G→T | Q231K (CAG→AAG) | ahr ← | aldehyde reductase, NADPH‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,495,519 | 0 | G | T | 95.7% | 154.4 / ‑3.3 | 47 | Q231K (CAG→AAG) | ahr | aldehyde reductase, NADPH‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (42/3); minor base C (0/2); total (42/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.25e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACAGCGGCGTGAGAACCGCACCGACCGTATGGAAATTA‑CCGCCATAGGTCAGCGCCTCAAAATAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCACCTCGCATCCCATTGCGTGCAGAAGTTT > NC_000913/4495405‑4495654 | acagCGGCGTGAGAACCGCACCGACCGTATGGAAATTA‑CCGCCATAGGTCAGCGCCTCAAAATAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGc > 2:915910/1‑140 (MQ=255)acagCGGCGTGAGAACCGCACCGACCGTATGGAAATTA‑CCGCCATAGGTCAGCGCCTCAAAATAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGc > 1:684315/1‑140 (MQ=255) cggcgTGAGAACCGCACCGACCGTATGGAAATTA‑CCGCCATAGGTCAGCGCCTCAAAATAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGAt > 2:344572/1‑140 (MQ=255) cgTGAGAACCGCACCGACCGTATGGAAATTA‑CCGCCATAGGTCAGCGCCTCAAAATAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATcgc > 2:760284/1‑140 (MQ=255) ccgaccgTATGGAAATTA‑CCGCCATAGGTCAGCGCCTCAAAATAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTcaccac > 1:489877/1‑140 (MQ=255) cccgttaggAAATTCCCCGCAATGGGTCAGCGCCTAAAAATAGGGCTGCCAGTCGAGGCTGACGTTGAGGGTGTTGAAAAGGAGACCAACCTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACtt < 2:173910/133‑1 (MQ=255) accgTATGGAAATTA‑CCGCCATAGGTCAGCGCCTCAAAATAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACttt > 1:676888/1‑140 (MQ=255) aaaatccccGCCATGGGTGAGCGCCTAAAAATAGGGCTGCCAGTCGAGGCTGACGTTGACGGTTTTGAAAATGAAATCAAACTCCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCAc < 2:957816/133‑1 (MQ=255) ccGCCATAGGTCAGCGCCTCAAAATAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGc > 2:1185982/1‑140 (MQ=255) ccATAGGTCAGCGCCTCAAAATAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGccgg > 1:1503680/1‑138 (MQ=255) ccATAGGTCAGCGCCTCAAAATAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAg > 1:487702/1‑140 (MQ=255) cATAGGTCGGCGCCTAAAAATAGGCCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAACCTCCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGc < 2:684315/140‑1 (MQ=255) cgcCTCAAAATAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGct > 1:261904/1‑140 (MQ=255) cgcCTCAAAATAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGct > 1:1078706/1‑140 (MQ=255) cgcCTCAAAATAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGct > 1:274622/1‑140 (MQ=255) cgcCTCAAAATAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGct > 1:537202/1‑140 (MQ=255) cgcCTCAAAATAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGct > 2:1092952/1‑140 (MQ=255) cgcCTCAAAATAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGct > 2:1314779/1‑140 (MQ=255) cgcCTCAAAATAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGct > 1:1195264/1‑140 (MQ=255) tCAAAATAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCtctgtctc > 1:1526715/1‑133 (MQ=255) cAAAATAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTc > 1:1079866/1‑140 (MQ=255) aaaTGGGGCTGCCAGTGGGGGCTGACTTTGGGGGTGTTGATAATGAAATCAACCTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGc < 2:777/140‑1 (MQ=255) aaaTGGGGCTGCCAGTCGGGGCTGGCGTTGAGGGTTTTGATAATGAGATAAACCTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGc < 2:603722/140‑1 (MQ=255) aaaTGGGGCTGCCAGTCGAGGCGGACGTTGAGGGTGTTGATAAGGAGATCAACCTCCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGc < 2:1195264/140‑1 (MQ=255) aaaTAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGc < 1:915910/140‑1 (MQ=255) aGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGa < 1:1185982/140‑1 (MQ=255) ggCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGAtt > 1:777/1‑140 (MQ=255) cTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAg > 2:703231/1‑140 (MQ=255) cTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAg > 1:581012/1‑140 (MQ=255) cTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAg > 1:603722/1‑140 (MQ=255) ccAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAAc > 2:438093/1‑140 (MQ=255) cAGTCGGGGCTGACGTTGCGGGTTTTAATAATGAAATAAACCTCCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACt < 1:760284/140‑1 (MQ=255) cGTTGGGGGTGTTGATAATGAGATCAAACTCCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCAcc < 2:1078706/140‑1 (MQ=255) cGTTGACGGTGTTGATAATGAGATCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCAcc > 2:116673/1‑140 (MQ=255) ttGACGGTGTTGATAATGAGATCAAACTCCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCACCTc < 1:116673/140‑1 (MQ=255) gACGGTGTTGATAATGAGATCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCACCTCGc > 1:433414/1‑140 (MQ=255) gACGGTGTTGATAATGAGATCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCACCTCGc < 2:489877/140‑1 (MQ=255) gtTAATAATGAAACCAACCTCCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCACCTCGCATCCCa < 1:344572/140‑1 (MQ=255) ttGATAATGAGATCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCACCTCGCATCCCAt > 1:1182424/1‑140 (MQ=255) gATAATGAGATCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCACCTCGCATCCCATTg > 1:869247/1‑140 (MQ=255) aaTGAGATCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCACCTCGCATCCCATTGCGt > 1:1404077/1‑140 (MQ=255) aTGAGATCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCACCTCGCATCCCATTGCGTg > 1:609949/1‑140 (MQ=255) tGAGATCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCACCTCGCATCCCATTGCGTGc < 1:703231/140‑1 (MQ=255) gagaTCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCACCTCGCATCCCATTGCGTGCa > 2:1143092/1‑140 (MQ=255) gagaTCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCACCTCGCATCCCATTGCGTGCa > 1:237080/1‑140 (MQ=255) gagaTCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCACCTCGCATCCCATTGCGTGCa > 2:854087/1‑140 (MQ=255) gagaTCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCACCTCGCATCCCATTGCGTGCa > 1:1009642/1‑140 (MQ=255) agacaaaaCTCCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCGGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCACCTCGCATCCCATTGCGTGCAg < 2:1503680/135‑1 (MQ=255) agaTCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCTCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCACCTCGCATCCCATTGCGTGCAg > 1:50514/1‑140 (MQ=255) agaTCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCACCTCGCATCCCATTGCGTGCAg > 1:1277884/1‑140 (MQ=255) agaTCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCACCTCGCATCCCATTGCGTGCAg < 1:1314779/140‑1 (MQ=255) gaTCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCACCTCGCATCCCATTGCGTGCAGa > 1:653317/1‑140 (MQ=255) gaTCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCACCTCGCATCCCATTGCGTGCAGa > 1:1492248/1‑140 (MQ=255) gaTCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCACCTCGCATCCCATTGCGTGCAGa > 1:1391657/1‑140 (MQ=255) gaTCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCACCTCGCATCCCATTGCGTGCAGa > 1:739333/1‑140 (MQ=255) tCAAACTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCACCTCGCATCCCATTGCGTGCAGAAg > 1:1453754/1‑140 (MQ=255) aaaCTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCACCTCGCATCCCATTGCGTGCAGAAGtt > 1:1278834/1‑140 (MQ=255) aaaCTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCACCTCGCATCCCATTGCGTGCAGAAGtt < 2:433414/140‑1 (MQ=255) aaCTTCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCACCTCGCATCCCATTGCGTGCAGAAGttt > 1:1421734/1‑140 (MQ=255) | ACAGCGGCGTGAGAACCGCACCGACCGTATGGAAATTA‑CCGCCATAGGTCAGCGCCTCAAAATAGGGCTGCCAGTCGAGGCTGACGTTGACGGTGTTGATAATGAGATCAAACTGCCCCGCCAGTGCTTTCAGTGCCTGCGGATCGCGGCTATTCACCACTTTATCGGCACCCATCGCCAGCACTTCCTGCTCTTTCGCCGGATTAGAACTAAAGGCTGTCACCTCGCATCCCATTGCGTGCAGAAGTTT > NC_000913/4495405‑4495654 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |