Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,893,720	G→T	intergenic (+9/‑413)	yoaB → / → yoaC	RutC family protein YoaB/DUF1889 domain‑containing protein YoaC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,893,720	0	G	T	100.0%	198.1 / NA	57	intergenic (+9/‑413)	yoaB/yoaC	RutC family protein YoaB/DUF1889 domain‑containing protein YoaC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (20/37);  total (20/37)

TTCGCGGCGATGAATAAAGCGTGGGATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGACCAGAGAATAAGATATTACTGTTGATGCTTCTGCTAATTCATTATTTATATTTATAATTTCAATTTTATCTA  >  NC_000913/1893583‑1893851                                                                                                                                          |                                                                                                                                    ttCGCGGCGATGAATAAAGCGTGGGATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTaa                                                                                                                                   >  1:72291/1‑140 (MQ=255)  tCGCGGCGATGAATAAAGCGTGGGATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAg                                                                                                                                  <  2:1388470/140‑1 (MQ=255)   cgcgGCGATGAATAAAGCGTGGGATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGTACAAGCGGGGTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGc                                                                                                                                 >  1:749280/1‑140 (MQ=255)            gAATAAAGCGTGGGATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGt                                                                                                                        <  2:950766/140‑1 (MQ=255)            gAATAAAGCGTGGGATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGt                                                                                                                        <  2:108285/140‑1 (MQ=255)             aaTAAAGCGTGGGATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTc                                                                                                                       <  2:458159/140‑1 (MQ=255)                 aaGCGTGGGATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGAc                                                                                                                   <  1:1202791/140‑1 (MQ=255)                  aGCGTGGGATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACg                                                                                                                  >  1:24180/1‑140 (MQ=255)                  aGCGTGGGATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACg                                                                                                                  >  2:1178448/1‑140 (MQ=255)                       gggATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTATGCTTTATCTAAGCAAAATAAGTCAGACGATAAt                                                                                                             >  2:1113276/1‑140 (MQ=255)                       gggATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAAt                                                                                                             >  2:42585/1‑140 (MQ=255)                       gggATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAAt                                                                                                             >  2:326341/1‑140 (MQ=255)                       gggATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAAt                                                                                                             >  2:266543/1‑140 (MQ=255)                         gATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAAttt                                                                                                           <  2:460152/140‑1 (MQ=255)                         gATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAAttt                                                                                                           <  2:604284/140‑1 (MQ=255)                         gATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAAttt                                                                                                           <  2:902405/140‑1 (MQ=255)                         gATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAAttt                                                                                                           <  2:989796/140‑1 (MQ=255)                                   ttGTCGCGGGTCATGCGCCGGTGCGCTGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAATTTATCGataata                                                                                                 <  1:42585/140‑1 (MQ=255)                                     gTCGCGGGTCATGCGCCGGTGCGCTGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAATTTATCGATAGTACt                                                                                               >  1:216027/1‑140 (MQ=255)                                     gTCGCGGGTCATGCGCCGGTGCGCTGCACGGTACAAGCGGGGTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAATTTATCGATAATACt                                                                                               >  2:1011987/1‑140 (MQ=255)                                       cgcgGGTCATGCGCCGGTGCGCTGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTgg                                                                                             >  1:958006/1‑140 (MQ=255)                                        gcgGGTCATGCGCCGGTGCGCTGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTATGCTTTATCTAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGt                                                                                            <  1:1113276/140‑1 (MQ=255)                                        gcgGGTCATGCGCCGGTGCGCTGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGt                                                                                            <  1:326341/140‑1 (MQ=255)                                        gcgGGTCATGCGCCGGTGCGCTGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGt                                                                                            <  2:371117/140‑1 (MQ=255)                                           ggTCATGCGCCGGTGCGCTGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCgg                                                                                         >  1:833564/1‑140 (MQ=255)                                                           gcTGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGAc                                                                         <  2:830342/140‑1 (MQ=255)                                                           gcTGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGAc                                                                         <  2:645792/140‑1 (MQ=255)                                                            cTGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGAcc                                                                        <  1:143329/140‑1 (MQ=255)                                                             tGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGACCa                                                                       <  2:749280/140‑1 (MQ=255)                                                             tGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGACCa                                                                       <  1:309878/140‑1 (MQ=255)                                                             tGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGACCa                                                                       <  1:1178448/140‑1 (MQ=255)                                                               cACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGACCaga                                                                     >  1:229415/1‑140 (MQ=255)                                                                  ggTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGACCAGAGaa                                                                  <  1:1011987/140‑1 (MQ=255)                                                                    tACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGACCAGAGAATa                                                                <  2:958006/140‑1 (MQ=255)                                                                          cGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGACCAGAGAATAAGatat                                                          <  2:1060519/140‑1 (MQ=255)                                                                           gggTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGACCAGAGAATAAGATAtt                                                         <  2:983771/140‑1 (MQ=255)                                                                           gggTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGACCAGAGAATAAGATAtt                                                         <  1:984557/140‑1 (MQ=255)                                                                           gggTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGACCAGAGAATAAGATAtt                                                         <  1:266543/140‑1 (MQ=255)                                                                              tttGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGACCAGAGAATAAGATATTa                                                        <  1:1139768/138‑1 (MQ=255)                                                                              tttGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGACCAGAGAATAAGATATTa                                                        >  2:1139768/1‑138 (MQ=255)                                                                              tttGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGACCAGAGAATAAGATATTACt                                                      >  1:605708/1‑140 (MQ=255)                                                                              tttGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGACCAGAGAATAAGATATTACt                                                      <  2:953714/140‑1 (MQ=255)                                                                              tttGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGACCAGAGAATAAGATATTACt                                                      >  2:1263143/1‑140 (MQ=255)                                                                                 gatgaACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGACCAGAGAATAAGATATTACTGtt                                                   <  2:605708/140‑1 (MQ=255)                                                                                       cccGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTATATAAATCGACCAGAGAATAAGATATTACTGTTGATGCt                                             <  2:562520/140‑1 (MQ=255)                                                                                       cccGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGACCAGAGAATAAGATATTa                                                        >  1:1335965/1‑129 (MQ=255)                                                                                       cccGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGACCAGAGAATAAGATATTa                                                        <  2:1335965/129‑1 (MQ=255)                                                                                       cccGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGACCAGAGAATAAGATATTACTGTTGATGCt                                             <  1:1263143/140‑1 (MQ=255)                                                                                       cccGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGACCAGAGAATAAGATATTACTGTTGATGCt                                             >  1:1387463/1‑140 (MQ=255)                                                                                       cccGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGACCAGAGAATAAGATATTACTGTTGATGCt                                             <  2:229415/140‑1 (MQ=255)                                                                                        ccGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGACCAGAGAATAAGATATTACTGTTGATGCtt                                            <  2:216027/140‑1 (MQ=255)                                                                                                  aaGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGACCAGAGAATAAGATATTACTGTTGATGCTTCTGCTAATTc                                  <  2:833564/140‑1 (MQ=255)                                                                                                        aaaTTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGACCAGAGAATAAGATATTACTGTTGATGCTTCt                                          <  2:465818/126‑1 (MQ=255)                                                                                                        aaaTTAAGATTGTGGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGACCAGAGAATAAGATATTACTGTTGATGCTTCt                                          >  1:465818/1‑126 (MQ=255)                                                                                                                    tgGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGACCAGAGAATAAGATATTACTGTTGATGCTTCTGCTAATTCATTATTTATATTTATAAt                >  2:570673/1‑140 (MQ=255)                                                                                                                    tgGCTGCGGTGTAAGCTTTATCTAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGACCAGAGAATAAGATATTACTGTTGATGCTTCTGCTAATTCATTATTTATATTTATAAt                <  2:24180/140‑1 (MQ=255)                                                                                                                                  gCTTTATCTAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGACCAGAGAATAAGATATTACTGTTGATGCTTCTGCTAATTCATTATTTATATTTATAATTTCAATTTTATCta  <  2:471891/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                    TTCGCGGCGATGAATAAAGCGTGGGATGCTTGGGTTGTCGCGGGTCATGCGCCGGTGCGCTGCACGGTACAAGCGGGTTTGATGAACCCGAAGTATAAAGTTGAAATTAAGATTGTGGCTGCGGTGTAAGCTTTATCGAAGCAAAATAAGTCAGACGATAATTTATCGATAATACTGGTCGGTTTTACATAAATCGACCAGAGAATAAGATATTACTGTTGATGCTTCTGCTAATTCATTATTTATATTTATAATTTCAATTTTATCTA  >  NC_000913/1893583‑1893851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑