Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,618	C→A	P273Q (CCG→CAG)	thrB →	homoserine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,618	0	C	A	100.0%	64.2 / NA	20	P273Q (CCG→CAG)	thrB	homoserine kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (8/12);  total (8/12)

AACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATC  >  NC_000913/3495‑3749                                                                                                                            |                                                                                                                                    aaCCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCAGGAAACCGCCCAgcgc                                                                                                                     >  1:378689/1‑140 (MQ=255)  aCCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCAGGAAACCGCCCAGcgcg                                                                                                                    <  1:25737/140‑1 (MQ=255)             aaCGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCAGGAAACCGCCCAGCGCGTTGCCGACTgg                                                                                                         >  2:583029/1‑140 (MQ=255)              aCGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCAGGAAACCGCCCAGCGCGTTGCCGACTGGt                                                                                                        >  1:180779/1‑140 (MQ=255)              aCGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCAGGAAACCGCCCAGCGCGTTGCCGACTGGt                                                                                                        <  2:378689/140‑1 (MQ=255)                          aGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCAGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACt                                                                                            <  1:721218/140‑1 (MQ=255)                           ggCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCAGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTa                                                                                           >  2:524890/1‑140 (MQ=255)                                ccGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCAGGAAACCGCCCAGCGCGTTGCCGACTGGTTgg                                                                                                     <  1:168733/125‑1 (MQ=255)                                ccGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCAGGAAACCGCCCAGCGCGTTGCCGACTGGTTgg                                                                                                     >  2:168733/1‑125 (MQ=255)                                               ggcgGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCAGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGtt                                                                       <  2:260986/140‑1 (MQ=255)                                                             tCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCAGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGc                                                         <  2:232943/140‑1 (MQ=255)                                                             tCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCAGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGc                                                         <  2:429427/140‑1 (MQ=255)                                                                  gcgGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCAGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCt                                                    >  1:290131/1‑140 (MQ=255)                                                                           agcgGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCAGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATAcggc                                           <  2:438156/140‑1 (MQ=255)                                                                                   ctccggctccggCCCGACCTTGTTCGCTCTGTGTGACAAGCAGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCAc                                   <  1:583029/140‑1 (MQ=255)                                                                                      cggctccggcCCGACCTTGTTCGCTCTGTGTGACAAGCAGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAg                                <  1:450333/140‑1 (MQ=255)                                                                                                   aCCTTGTTCGCTCTGTGTGACAAGCAGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCAc                                   >  2:676250/1‑124 (MQ=255)                                                                                                   aCCTTGTTCGCTCTGTGTGACAAGCAGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCAc                                   <  1:676250/124‑1 (MQ=255)                                                                                                      ttGTTCGCTCTGTGTGACAAGCAGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGGGCGGGAACTGGGAAAAAAAAt                >  2:135284/1‑140 (MQ=255)                                                                                                                    agACAGGCAGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATc  <  1:37387/139‑1 (MQ=255)                                                                                                                            |                                                                                                                                    AACCCTACCGTGAACGGTTACTGCCAGGCTTCCGGCAGGCGCGGCAGGCGGTCGCGGAAATCGGCGCGGTAGCGAGCGGTATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATC  >  NC_000913/3495‑3749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑