Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,601,623	C→A	T141T (ACC→ACA)	hyfA →	hydrogenase 4 component A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,601,623	0	C	A	100.0%	110.4 / NA	33	T141T (ACC→ACA)	hyfA	hydrogenase 4 component A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (17/16);  total (17/16)

ATCAGCGCTTCAGGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACCGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCC  >  NC_000913/2601489‑2601758                                                                                                                                       |                                                                                                                                        aTCAGCGCTTCAGGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTcgc                                                                                                                                    <  2:55718/140‑1 (MQ=255)  tCAGCGCTTCAGGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTcgcg                                                                                                                                   <  2:55922/140‑1 (MQ=255)              gAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGAc                                                                                                                       >  1:149453/1‑140 (MQ=255)                     tCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAATGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCtgtgtg                                                                                                                >  2:522260/1‑140 (MQ=255)                     tCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCtgtgtg                                                                                                                >  2:552865/1‑140 (MQ=255)                     tCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCtgtgtg                                                                                                                >  2:53546/1‑140 (MQ=255)                     tCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCtgtgtg                                                                                                                >  2:343161/1‑140 (MQ=255)                                   aTGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGaa                                                                                                  >  2:436460/1‑140 (MQ=255)                                           tatGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGc                                                                                          >  2:27842/1‑140 (MQ=255)                                                  ttCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCt                                                                                        >  2:65683/1‑135 (MQ=255)                                                  ttCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCt                                                                                        <  1:65683/135‑1 (MQ=255)                                                      ggCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTcgcg                                                                               <  2:149453/140‑1 (MQ=255)                                                      ggCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTcgcg                                                                               <  1:27842/140‑1 (MQ=255)                                                             ggCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGccc                                                                        <  1:359217/140‑1 (MQ=255)                                                                  actcaAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATc                                                                   >  2:215206/1‑140 (MQ=255)                                                                        aGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGt                                                             <  1:436460/140‑1 (MQ=255)                                                                             gCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACgg                                                        <  1:123828/140‑1 (MQ=255)                                                                                 agaaAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGa                                                    <  1:522260/140‑1 (MQ=255)                                                                                              acacaacaTGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAg                                       <  1:215206/140‑1 (MQ=255)                                                                                                  aacaTGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTg                                   <  1:9500/140‑1 (MQ=255)                                                                                                    caTGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCa                                 >  1:307821/1‑140 (MQ=255)                                                                                                        cTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTg                                           >  2:378914/1‑126 (MQ=255)                                                                                                        cTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTg                                           <  1:378914/126‑1 (MQ=255)                                                                                                        cTTTGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGt                             >  2:562566/1‑140 (MQ=255)                                                                                                          ttGCTGCGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCa                           <  1:53546/140‑1 (MQ=255)                                                                                                            gctgcGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGa                         >  2:674219/1‑140 (MQ=255)                                                                                                             ctgcGCTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGAt                        <  2:676919/140‑1 (MQ=255)                                                                                                             ctgcGCTGGGAACCTGGTGGCCAGACAGTCGCTGTGAAATGGGACCTGTGTGATTTCTTGCCAGAAGGTCGGGCTTGCGTTCGCGCTTGCCAGAATCAGGCGTTGCGGCTGATTTCCGGTGATAGCCGGCAACGTCAGAt                        <  2:311180/140‑1 (MQ=255)                                                                                                                cgcTGGGAACCTGGTGTCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATgaa                     >  1:451751/1‑140 (MQ=255)                                                                                                                             gtgtCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAgcgc        >  1:150751/1‑140 (MQ=255)                                                                                                                              tgtCCAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGcc       <  1:674219/140‑1 (MQ=255)                                                                                                                                  cAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCt      >  2:156446/1‑137 (MQ=255)                                                                                                                                  cAGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCt      <  1:156446/137‑1 (MQ=255)                                                                                                                                   aGACAGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGcc  >  1:393459/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        ATCAGCGCTTCAGGAAGCCGTCCGGTGAATGCCCATGCGCAATATGTTTTTCAGGCTGAAGGCTCACTCAAAGACGGCGAAGAAAACGCGCCAACACAACATGCTTTGCTGCGCTGGGAACCTGGTGTCCAGACCGTCGCGGTGAAATGCGACCTGTGTGATTTCTTGCCAGAAGGTCCGGCCTGCGTTCGCGCTTGCCCGAATCAGGCGTTACGGCTGATCACCGGTGATAGCCTGCAACGTCAGATGAAAGAAAAACAGCGCCTTGCC  >  NC_000913/2601489‑2601758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑