Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,698,293	G→T	F86L (TTC→TTA)	pgpC ←	phosphatidylglycerophosphatase C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,698,293	0	G	T	100.0%	174.7 / NA	51	F86L (TTC→TTA)	pgpC	phosphatidylglycerophosphatase C
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (25/26);  total (25/26)

TCGAAATAAACCGCTTCAACCAGCGGCTGCGGAGAGCCGGTAATCAACCAGATATCAGCATCGGAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGGCTATAATCGGTAACAACGGCA  >  NC_000913/2698156‑2698426                                                                                                                                          |                                                                                                                                        tCGAAATAAACCGCTTCAACCAGCGGCTGCGGAGAGCCGGTAATCAACCAGATATCAGCATCGGAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTaa                                                                                                                                       >  2:287501/1‑140 (MQ=255)  cGAAATAAACCGCTTCAACCAGCGGCTGCGGAGAGCCGGTAATCAACCAGATATCAGCATCGGAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTaaa                                                                                                                                      <  2:273039/140‑1 (MQ=255)           ccGCTTCAACCAGCGGCTGCGGAGAGCCGGTAATCAACCAGATATCAGCATCGGAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCTTGc                                                                                                                             <  2:548279/140‑1 (MQ=255)               ttCAACCAGCGGCTGCGGAGAGCCGGTAATCAACCAGATATCAGCATCGGAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACg                                                                                                                         <  2:511350/140‑1 (MQ=255)                      aGCGGCTGCGGAGAGCCGGTAATCAACCAGATATCAGCATCGGAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTaa                                                                                                                  >  2:96369/1‑140 (MQ=255)                             gCGGAGAGCCGGTAATCAACCAGATATCAGCATCGGAACTTTACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTCACATTGTCGCGAAACCAGCGCACTAAATCGGCATGCAACGTCTGTAAACGTGCt                                                                                                           <  2:710411/140‑1 (MQ=255)                                     ccGGTAATCAACCAGATATCAGCATCGGAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCt‑gtg                                                                                                  >  1:574007/1‑140 (MQ=255)                                     acGGTGATAAATCAGATATCGGCATCGGAGCTTAACGGGGAGGTGTGGAATCGCTCCTGAACCAGCGGGAAGGCGGTAACGTTGTCGCGAAACCAGGCCACTAAATCGGCCTGCAACGTCTGGAAACGAGCTTCGCt‑gtg                                                                                                  >  2:546015/2‑140 (MQ=255)                                        gTAATCAACCAGATATCAGCATCGGAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGAcc                                                                                               >  2:673565/1‑140 (MQ=255)                                             cAACCAGATATCAGCATCGGAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAg                                                                                          >  1:245516/1‑140 (MQ=255)                                               aCCAGATATCAGCATCGGAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTg                                                                                        <  1:223376/140‑1 (MQ=255)                                               aCCAGATATCAGCATCGGAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTg                                                                                        <  2:245516/140‑1 (MQ=255)                                                ccAGATATCAGCATCGGAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGc                                                                                       >  2:317937/1‑140 (MQ=255)                                                     tatCAGCATCGGAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCAcccc                                                                                  >  2:159673/1‑140 (MQ=255)                                                       tCAGCATCGGAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCca                                                                                <  1:159673/140‑1 (MQ=255)                                                              cGGAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGc                                                                        >  1:600491/1‑140 (MQ=255)                                                              cGGAACTTAACAGGTAGGTGGATAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGc                                                                        >  2:597156/1‑140 (MQ=255)                                                                gAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAg                                                                      <  2:574007/140‑1 (MQ=255)                                                                gAACTTAACAGGTAGGTGGATAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAg                                                                      <  1:597156/140‑1 (MQ=255)                                                                           gtaggtGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGcc                                                           >  1:660622/1‑140 (MQ=255)                                                                              ggtggtTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGc                                                        >  2:93127/1‑140 (MQ=255)                                                                                  gtTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACTTTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGc                                                    >  2:709648/1‑140 (MQ=255)                                                                                  gtTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGc                                                    >  2:458611/1‑140 (MQ=255)                                                                                  gtTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGc                                                    >  2:40511/1‑140 (MQ=255)                                                                                  gtTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACG‑GCGTCGCTGGGGACCAAAAGGGC‑CCCCCACAAAAAGAAACCTCACCGGCAAGCGtcc                                                    >  2:7246/1‑138 (MQ=255)                                                                                      aTCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCa                                                <  2:532370/140‑1 (MQ=255)                                                                                        cGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACg                                              <  2:86536/140‑1 (MQ=255)                                                                                         gCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGa                                             >  1:721928/1‑140 (MQ=255)                                                                                         gCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGa                                             >  2:592234/1‑140 (MQ=255)                                                                                          cTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACgcc                                            <  1:546015/140‑3 (MQ=255)                                                                                          cTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGAc                                            >  2:303303/1‑140 (MQ=255)                                                                                          cTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGAc                                            <  2:612500/140‑1 (MQ=255)                                                                                          cTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGAc                                            <  1:233806/140‑1 (MQ=255)                                                                                          cTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGAc                                            <  1:96369/140‑1 (MQ=255)                                                                                            ccTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCt                                          >  1:260691/1‑140 (MQ=255)                                                                                               gAACCAGCGGAAAGGCGGTAACTTTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCtttt                                       <  1:709648/140‑1 (MQ=255)                                                                                               gAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCtttt                                       <  1:458611/140‑1 (MQ=255)                                                                                                     gCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAAt                                 <  2:303827/140‑1 (MQ=255)                                                                                                     gCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAAt                                 >  1:279008/1‑140 (MQ=255)                                                                                                     gCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAAt                                 >  1:573621/1‑140 (MQ=255)                                                                                                     gCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAAt                                 >  1:303827/1‑140 (MQ=255)                                                                                                     gCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAAt                                 <  1:40511/140‑1 (MQ=255)                                                                                                       ggAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAAt                                 >  2:302701/1‑138 (MQ=255)                                                                                                       ggAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAAt                                 <  1:302701/138‑1 (MQ=255)                                                                                                       ggAAAGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATaa                               >  2:572071/1‑140 (MQ=255)                                                                                                           aGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCa                           <  2:419187/140‑1 (MQ=255)                                                                                                           aGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCa                           <  2:260691/140‑1 (MQ=255)                                                                                                           aGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCa                           <  2:660622/140‑1 (MQ=255)                                                                                                           aGGCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGAATCATCGGCCAGCGTGCCGCACGCCCTTTTATCAATAACGCa                           <  2:234079/140‑1 (MQ=255)                                                                                                            ggCGGTAACATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCaa                          <  1:673565/140‑1 (MQ=255)                                                                                                                  aaCATTGTCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGGCta                    <  2:279008/140‑1 (MQ=255)                                                                                                                         tCGCGAAACCAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGGCTATAATCgg             >  2:331191/1‑140 (MQ=255)                                                                                                                                  cAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGGCTATAATCGGTAACAACgg    <  2:721928/140‑1 (MQ=255)                                                                                                                                  cAGCGCACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGGCTATAATCGGTAACAACgg    <  2:600491/140‑1 (MQ=255)                                                                                                                                    gcgcACTAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGGCTATAATCGGTAACAACGGCa  <  1:287501/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        TCGAAATAAACCGCTTCAACCAGCGGCTGCGGAGAGCCGGTAATCAACCAGATATCAGCATCGGAACTTAACAGGTAGGTGGTTAATCGCTCCTGAACCAGCGGAAAGGCGGTAACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCT‑GTGACCAAAAGTGCACCCCCAC‑AGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGGCTATAATCGGTAACAACGGCA  >  NC_000913/2698156‑2698426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑