Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,098,079	G→T	V271V (GTG→GTT)	yggW →	putative coproporphyrinogen‑III oxidase‑like protein YggW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,098,079	0	G	T	100.0%	193.1 / NA	56	V271V (GTG→GTT)	yggW	putative coproporphyrinogen‑III oxidase‑like protein YggW
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (29/27);  total (29/27)

TATTAACCGCAGCGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCG  >  NC_000913/3097946‑3098210                                                                                                                                      |                                                                                                                                    tattaACCGCAGCGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTc                                                                                                                               >  2:190601/1‑140 (MQ=255)     aaCCGCAGCGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCgg                                                                                                                           >  1:538276/1‑140 (MQ=255)          cAGCGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATggg                                                                                                                      >  2:21214/1‑140 (MQ=255)           aGCGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGgc                                                                                                                     >  2:53605/1‑140 (MQ=255)                 ttATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTc                                                                                                               >  2:515822/1‑140 (MQ=255)                          atatGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTacca                                                                                                      >  2:719087/1‑140 (MQ=255)                             tGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTAccacca                                                                                                   >  2:5434/1‑140 (MQ=255)                              gAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCTGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCaa                                                                                                  >  2:623989/1‑140 (MQ=255)                              gAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCaa                                                                                                  <  1:190601/140‑1 (MQ=255)                                aaCTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCaaaa                                                                                                >  2:276108/1‑140 (MQ=255)                                     ccGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGt                                                                                           >  2:342324/1‑140 (MQ=255)                                      cGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTc                                                                                          <  2:586325/140‑1 (MQ=255)                                      cGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTc                                                                                          <  1:42751/140‑1 (MQ=255)                                        cTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCAt                                                                                        >  2:124047/1‑140 (MQ=255)                                             gCCAAACCCGGTTATCAGTGCCTGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCcgcg                                                                                   <  1:623989/140‑1 (MQ=255)                                               cAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTg                                                                                 <  1:315014/140‑1 (MQ=255)                                                 aaCCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGt                                                                               <  1:82853/140‑1 (MQ=255)                                                       gTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCAt                                                                                        >  1:402770/1‑125 (MQ=255)                                                       gTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCAt                                                                                        <  2:402770/125‑1 (MQ=255)                                                       gTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATg                                                                         >  2:58/1‑140 (MQ=255)                                                          aTCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCaa                                                                      <  2:136382/140‑1 (MQ=255)                                                           tCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCaag                                                                     >  1:50284/1‑140 (MQ=255)                                                             aGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCaagga                                                                   <  1:5434/140‑1 (MQ=255)                                                             aGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCaagga                                                                   <  1:173684/140‑1 (MQ=255)                                                             aGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCaagga                                                                   <  1:719087/140‑1 (MQ=255)                                                                  cAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTa                                                              <  1:276108/140‑1 (MQ=255)                                                                       caATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTgg                                                         <  1:58/140‑1 (MQ=255)                                                                       caATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTgg                                                         <  1:21214/140‑1 (MQ=255)                                                                         aTCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCaaggaa                                                                  >  2:162676/1‑129 (MQ=255)                                                                         aTCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCaaggaa                                                                  <  1:162676/129‑1 (MQ=255)                                                                         aTCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGaa                                                       >  2:677048/1‑140 (MQ=255)                                                                                  actGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGt                                              <  2:447164/140‑1 (MQ=255)                                                                                    tGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCTAGGAAGGTATCTGGAAAGCCAGCGTGa                                            >  2:73507/1‑140 (MQ=255)                                                                                    tGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGa                                            >  1:545983/1‑140 (MQ=255)                                                                                      gcgcTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATg                                          >  1:229522/1‑140 (MQ=255)                                                                                      gcgcTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATg                                          >  2:541180/1‑140 (MQ=255)                                                                                        gcTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCCTCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTc                                        <  2:538276/140‑1 (MQ=255)                                                                                        gcTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTc                                        <  1:515822/140‑1 (MQ=255)                                                                                         cTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCg                                       <  1:124047/140‑1 (MQ=255)                                                                                         cTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCg                                       <  1:181563/140‑1 (MQ=255)                                                                                         cTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCg                                       <  1:342324/140‑1 (MQ=255)                                                                                          tttGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGa                                      >  1:590925/1‑140 (MQ=255)                                                                                          tttGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGa                                      >  2:309654/1‑140 (MQ=255)                                                                                             ggTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGc                                   <  2:50284/140‑1 (MQ=255)                                                                                             ggTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGc                                   <  1:677048/140‑1 (MQ=255)                                                                                                     cATCGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATa                           <  2:1239/140‑1 (MQ=255)                                                                                                        cGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGc                        <  1:309654/140‑1 (MQ=255)                                                                                                        cGGTATTGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGc                        <  1:541180/140‑1 (MQ=255)                                                                                                             ttGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGttt                   >  2:115899/1‑140 (MQ=255)                                                                                                             ttGGCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGttt                   >  1:249817/1‑140 (MQ=255)                                                                                                                gCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCca                                 >  2:380189/1‑123 (MQ=255)                                                                                                                gCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCca                                 <  1:380189/123‑1 (MQ=255)                                                                                                                gCTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAg                >  2:595037/1‑140 (MQ=255)                                                                                                                 cTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGt               >  1:361717/1‑140 (MQ=255)                                                                                                                 cTGCGGCGCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGt               <  2:590925/140‑1 (MQ=255)                                                                                                                    cggcgCACACGGCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCt            >  1:524707/1‑140 (MQ=255)                                                                                                                              ggCAAAGTTACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCAGAGATAAGCCGTTTGAGTTCTTTATGAATCg  >  1:171781/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                    TATTAACCGCAGCGGGTTATCAGCAATATGAAACTTCCGCTTACGCCAAACCCGGTTATCAGTGCCAGCACAATCTCAACTACTGGCGCTTTGGTGACTACATCGGTATTGGCTGCGGCGCACACGGCAAAGTGACCTTCCCGGATGGGCGCATTCTGCGTACCACCAAAACGCGTCATCCGCGTGGTTTTATGCAAGGAAGGTATCTGGAAAGCCAGCGTGATGTCGAAGCCACAGATAAGCCGTTTGAGTTCTTTATGAATCG  >  NC_000913/3097946‑3098210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑