Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,846,748	G→T	G140G (GGC→GGA)	uhpT ←	hexose‑6‑phosphate:phosphate antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,846,748	0	G	T	100.0%	206.3 / NA	59	G140G (GGC→GGA)	uhpT	hexose‑6‑phosphate:phosphate antiporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (27/32);  total (27/32)

GGTAATTTGCCCCGAACAGCGCCACACCTGCTGCGCCTGCACCGCCAAGGTTGTGAGAAATATTCCAGAAACCGAGGAATGTCCCGCGTTTACGACGCGGCGTCCATTTGGTGATGGTGGAGTAACTGCACGAACCGCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCGCTGCCCATACTGGCACTGAAGCCCAGCATACAAATAGCAGAGAGGATCAGCATGAACGGCAGGAATTGTT  >  NC_000913/3846612‑3846884                                                                                                                                         |                                                                                                                                         ggTAATTTGCCCCGAACAGCGCCACACCTGCTGCGCCTGCACCGCCAAGGTTGTGAGAAATATTCCAGAAACCGAGGAATGTCCCGCGTTTACGACGCGGCGTCCATTTGGTGATGGTGGAGTAACTGCACGAAcctccg                                                                                                                                       >  2:507027/1‑140 (MQ=255)       ttGCCCCGAACAGCGCCACACCTGCTGCGCCTGCACCGCCAAGGTTGTGAGAAATATTCCAGAAACCGAGGAATGTCCCGCGTTTACGACGCGGCGTCCATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTActc                                                                                                                                 <  2:671944/140‑1 (MQ=255)       ttGCCCCGAACAGCGCCACACCTGCTGCGCCTGCACCGCCAAGGTTGTGAGAAATATTCCAGAAACCGAGGAATGTCCCGCGTTTACGACGCGGCGTCCATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTActc                                                                                                                                 >  1:172596/1‑140 (MQ=255)               aaCAGCGCCACACCTGCTGCGCCTGTACCGCCAAGGTTGTGAGAAATATTCCAGAAACCGAGGAATGTCCCGCGTTTACGACGCGGCGTCCATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGaaaaa                                                                                                                         <  2:646828/140‑1 (MQ=255)               aaCAGCGCCACACCTGCTGCGCCTGCACCGCCAAGGTTGTGAGAAATATTCCAGAAACCGAGGAATGTCCCGCGTTTACGACGCGGCGTCCATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGaaaaa                                                                                                                         >  1:660055/1‑140 (MQ=255)                aCAGCGCCACACCTGCTGCGCCTGCACCGCCAAGGTTGTGAGAAATATTCCAGAAACCGAGGAATGTCCCGCGTTTACGACGCGGCGTCCATTTGGTGATGGTGGAGTAACTGCACGAANCTCNGGTACTCNGGAAAAAg                                                                                                                        <  1:487251/140‑1 (MQ=255)                 cAGCGCCACCCCTGCTGCGCCTGCACCGCCAAGGTTGTGAGAAATATTCCAGAAACCGAGGAATGTCCCGCGTTTACGACGCGGCGTCCATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGATAAAGc                                                                                                                       <  2:545905/140‑1 (MQ=255)                    cgcCACACCTGCTGCGCCTGCACCGCCAAGGTTGTGAGAAATATTCCAGAAACCGAGGAATGTCCCGCGTTTACGACGCGGCGTCCATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGc                                                                                                                    >  2:254613/1‑140 (MQ=255)                     gcCACACCTGCTGCGCCTGCACCGCCAAGGTTGTGAGAAATATTCCAGAAACCGAGGAATGTCCCGCGTTTACGACGCGGCGTCCATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCt                                                                                                                   <  2:507864/140‑1 (MQ=255)                              gctgcGCCTGCACCGCCAAGGTTGTGAGAAATATTCCAGAAACCGAGGAATGTCCCGCGTTTACGACGCGGCGTCCATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCt                                                                                                                   >  2:306791/1‑131 (MQ=255)                              gctgcGCCTGCACCGCCAAGGTTGTGAGAAATATTCCAGAAACCGAGGAATGTCCCGCGTTTACGACGCGGCGTCCATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCt                                                                                                                   <  1:306791/131‑1 (MQ=255)                                    ccTGCACCGCCAAGGTTGTGAGAAATATTCCAGAAACCGAGGAATGTCCCGCGTTTACGACGCGGCGTCCATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGc                                                                                                    >  2:111620/1‑140 (MQ=255)                                     cTGCACCGCCAAGGTTGTGAGAAATATTCCAGAAACCGAGGAATGTCCCGCGTTTACGACGCGGCGTCCATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCa                                                                                                   >  1:534459/1‑140 (MQ=255)                                      tGCACCGCCAAGGTTGTGAGAAATATTCCAGAAACCGAGGAATGTCCCGCGTTTACGACGCGGCGTCCATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCaa                                                                                                  <  1:254613/140‑1 (MQ=255)                                      tGCACCGCCAAGGTTGTGAGAAATATTCCAGAAACCGAGGAATGTCCCGCGTTTACGACGCGGCGTCCATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCaa                                                                                                  <  2:534459/140‑1 (MQ=255)                                      tGCACCGCCAAGGTTGTGAGAAATATTCCAGAAACCGAGGAATGTCCCGCGTTTACGACGCGGCGTCCATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCaa                                                                                                  <  1:549586/140‑1 (MQ=255)                                                   ttGTGAGAAATATTCCAGAAACCGAGGAATGTCCCGCGTTTACGACGCGGCGTCCATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAg                                                                                     <  2:172596/140‑1 (MQ=255)                                                     gtgAGAAATATTCCAGAAACCGAGGAATGTCCCGCGTTTACGACGCGGCGTCCATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGc                                                                                   >  2:8636/1‑140 (MQ=255)                                                     gtgAGAAATATTCCAGAAACCGAGGAATGTCCCGCGTTTACGACGCGGCGTCCATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGc                                                                                   >  2:260636/1‑140 (MQ=255)                                                           aaTATTCCAGAAACCGAGGAATGTCCCGCGTTTACGACGCGGCGTCCATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCg                                                                             <  2:151047/140‑1 (MQ=255)                                                                tCCAGAAACCGAGGAATGTCCCGCGTTTACGACGCGGCGTCCATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCg                                                                        <  1:8636/140‑1 (MQ=255)                                                                tCCAGAAACCGAGGAATGTCCCGCGTTTACGACGCGGCGTCCATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCg                                                                        <  1:507027/140‑1 (MQ=255)                                                                 ccAGAAACCGAGGAATGTCCCGCGTTTACGACGCGGCGTCCATTTGGTTATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCGc                                                                       >  1:369464/1‑140 (MQ=255)                                                                 ccAGAAACCGAGGAATGTCCCGCGTTTACGACGCGGCGTCCATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCGc                                                                       <  2:622893/140‑1 (MQ=255)                                                                     aaaCCGAGGAATGTCCCGCGTTTACGACGCGGCGTCCATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCGCTGcc                                                                   <  2:704677/140‑1 (MQ=255)                                                                         cGAGGAATGTCCCGCGTTTACGACGCGGCGTCCATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGcc                                                                         >  1:194902/1‑130 (MQ=255)                                                                         cGAGGAATGTCCCGCGTTTACGACGCGGCGTCCATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGcc                                                                         <  2:194902/130‑1 (MQ=255)                                                                           aGGAATGTCCCGCGTTTACGACGCGGCGTCCATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCg                                                                        >  2:398241/1‑129 (MQ=255)                                                                           aGGAATGTCCCGCGTTTACGACGCGGCGTCCATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCg                                                                        <  1:398241/129‑1 (MQ=255)                                                                           aGGAATGTCCCGCGTTTACGACGCGGCGTCCATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCGCTGCCCATACt                                                             >  1:461204/1‑140 (MQ=255)                                                                                       cgTTTACGACGCGGCGTCCATTTGGTTATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCGCTGCCCATACTGGCACTGAAGcc                                                 <  2:369464/140‑1 (MQ=255)                                                                                       cgTTTACGACGCGGCGTCCATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCGCTGCCCATACTGGCACTGAAGcc                                                 <  1:111620/140‑1 (MQ=255)                                                                                       cgTTTACGACGCGGCGTCCATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCGCTGCCCATACTGGCACTGAAGcc                                                 <  2:329092/140‑1 (MQ=255)                                                                                        gTTTACGACGCGGCGTCCATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCGCTGCCCATAc                                                              <  2:598456/126‑1 (MQ=255)                                                                                        gTTTACGACGCGGCGTCCATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCGCTGCCCATAc                                                              >  1:598456/1‑126 (MQ=255)                                                                                        gTTTACGACGCGGCGTCCATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCGCTGCCCATACTGGCACTGAAGccc                                                <  1:235481/140‑1 (MQ=255)                                                                                          ttACGACGCGGCGTCCATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCGCTGCCCATACTGGCACTGAAGCCCAg                                              >  1:112677/1‑140 (MQ=255)                                                                                           tACGACGCGGCGTCCATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCGCTGCCCATACTGGCACTGAAGCCCAGc                                             >  2:490471/1‑140 (MQ=255)                                                                                                cgcgGCGTCCATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCGCTGCCCATACTGGCACTGAAGCCCAGCATACa                                        <  1:260636/140‑1 (MQ=255)                                                                                                  cggcgTCCATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCGCTGCCCATACTGGCACTGAAGCCCAGCATACaaa                                      >  1:450102/1‑140 (MQ=255)                                                                                                  cggcgTCCATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCGCTGCCCATACTGGCACTGAAGCCCAGCATACaaa                                      <  1:490471/140‑1 (MQ=255)                                                                                                    gcgTCCATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCGCTGCCCATACTGGCACTGAAg                                                   >  2:251787/1‑125 (MQ=255)                                                                                                    gcgTCCATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCGCTGCCCATACTGGCACTGAAg                                                   <  1:251787/125‑1 (MQ=255)                                                                                                     cgTCCATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCGCTGCCCATACTGGCACTGAAGCCCAGCATACAAATAg                                   <  2:660055/140‑1 (MQ=255)                                                                                                     cgTCCATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCGCTGCCCATACTGGCACTGAAGCCCAGCATACAAATAg                                   <  2:450102/140‑1 (MQ=255)                                                                                                     cgTCCATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCGCTGCCCATACTGGCACTGAAGCCCAGCATACAAATAg                                   <  2:112677/140‑1 (MQ=255)                                                                                                         gacagggTGATGGTGGAGTAAATGCCCGACCCTGCGGTAGTCGAGAAAAAGCCGATTAAGGCGTAGAAGGCAATCAGCGGGAACAGGATAACCGAGCCGCTGACCATACTGGCACTGAAGCCCAGCATACAAATAGCaga                               <  2:43938/135‑1 (MQ=255)                                                                                                         cATTTGGTGATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCGCTGCCCATACTGGCACTGAAGCCCAGCATACAAATAGCaga                               <  2:286299/140‑1 (MQ=255)                                                                                                               gTGATGGTGGAGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCGCTGCCCATACTGGCACTGAAGCCCAGCATACAAATAGCAGAGAGGAt                         >  2:635590/1‑140 (MQ=255)                                                                                                                         aGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCGCTGCCCATACTGGCACTGAAGCCCAGCATACAAATAGCAGAGAGGATCAGCactgtc               >  1:43938/1‑135 (MQ=255)                                                                                                                         aGTAACTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCGCTGCCCATACTGGCACTGAAGCCCAGCATACAAATAGCAGAGAGGATCAGCATGAAc               <  2:461204/140‑1 (MQ=255)                                                                                                                              cTGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCGCTGCCCATACTGGCACTGAAGCCCAGCATACAAATAGCAGAGAGGATCAGCATGAACGGCAg          >  2:384993/1‑140 (MQ=255)                                                                                                                               tGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCGCTGCCCATACTGGCACTGAAGCCCAGCATACAAATAGCAGAGAGGATCAGCATGAACGGCAgg         >  2:408407/1‑140 (MQ=255)                                                                                                                               tGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCGCTGCCCATACTGGCACTGAAGCCCAGCATACAAATAGCAGAGAGGATCAGCATGAACGGCAgg         >  2:512589/1‑140 (MQ=255)                                                                                                                               tGCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCGCTGCCCATACTGGCACTGAAGCCCAGCATACAAATAGCAGAGAGGATCAGCATGAACGGCAgg         >  2:357278/1‑140 (MQ=255)                                                                                                                                gCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCGCTGCCCATACTGGCACTGAAGCCCAGCATACAAATAGCAGAGAGGATCAGCa                    <  2:477796/128‑1 (MQ=255)                                                                                                                                gCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCGCTGCCCATACTGGCACTGAAGCCCAGCATACAAATAGCAGAGAGGATCAGCa                    >  1:477796/1‑128 (MQ=255)                                                                                                                                gCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCGCTGCCCATACTGGCACTGAAGCCCAGCATACAAATAGCAGAGAGGATCAGCATGAACGGCAGGa        >  2:665608/1‑140 (MQ=255)                                                                                                                                gCACGAACCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCGCTGCCCATACTGGCACTGAAGCCCAGCATACAAATAGCAGAGAGGATCAGCATGAACGGCAGGa        >  2:283602/1‑140 (MQ=255)                                                                                                                                     aaCCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCGCTGCCCATACTGGCACTGAAGCCCAGCATACAAATAGCAGAGAGGATCAGCATGAACGGCAGGAATTGt   >  1:575835/1‑140 (MQ=255)                                                                                                                                      aCCTCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCGCTGCCCATACTGGCACTGAAGCCCAGCATACAAATAGCAGAGAGGATCAGCATGAACGGCAGGAATTGtt  <  2:575835/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         GGTAATTTGCCCCGAACAGCGCCACACCTGCTGCGCCTGCACCGCCAAGGTTGTGAGAAATATTCCAGAAACCGAGGAATGTCCCGCGTTTACGACGCGGCGTCCATTTGGTGATGGTGGAGTAACTGCACGAACCGCCGGTACTCTGGAAAAAGCCGCTTAAGGCGTAGAAGGCAATCATCAGGAACAGGCTAACCGAGCCGCTGCCCATACTGGCACTGAAGCCCAGCATACAAATAGCAGAGAGGATCAGCATGAACGGCAGGAATTGTT  >  NC_000913/3846612‑3846884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑