Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,847,131	G→T	L13M (CTG→ATG)	uhpT ←	hexose‑6‑phosphate:phosphate antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,847,131	0	G	T	100.0%	130.9 / NA	38	L13M (CTG→ATG)	uhpT	hexose‑6‑phosphate:phosphate antiporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (17/21);  total (17/21)

TTCTGCGCGATGTTAAAGTTCTTGCGAATCAGGTACATCGTCAGGTAGCCGATAAAGACCACCAGGTAGGATTGCATGAACGGTTTGAACCACATTTTGCGCCGCACTTCGAGCGGAAGGTCCAGGGTCGGCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGGAA  >  NC_000913/3847007‑3847260                                                                                                                             |                                                                                                                                  ttCTGCGCGATGTTAAAGTTCTTGCGAATCAGGTACATCGTCAGGTAGCGGATAAAGGCCACCAGGTAGGATTGCATGAACGGTTTGAACCACATTTTGCGGCGCGCTTCGAGCGGAAGGTCCATGGTCGGCTTGCGAAc                                                                                                                    <  2:637418/140‑1 (MQ=255) ttCTGCGCGATGTTAAAGTTCTTGCGAATCAGGTACATCGTCAGGTAGCCGATAAAGACCACCAGGTAGGATTGCATGAACGGTTTGAACCACATTTTGCGCCGCACTTCGAGCGGAAGGTCCATGGTCGGCTTGCGAAc                                                                                                                    >  1:474377/1‑140 (MQ=255)    tGCGCGATGTTAAAGTTCTTGCGAATCAGGTACATCGTCAGGTAGCCGATAAAGACCACCAGGTAGGATTGCATGAACGGTTTGAACCACATTTTGCGCCGCACTTCGAGCGGAAGGTCCATGGTCGGCTTGCGAACCTg                                                                                                                 >  2:629938/1‑140 (MQ=255)           tGTTAAAGTTCTTGCGAATCAGGTACATCGTCAGGTAGCCGATAAAGACCACCAGGTAGGATTGCATGAACGGTTTGAACCACATTTTGCGCCGCACTTCGAGCGGAAGGTCCATGGTCGGCTTGCGAACCTGGTTTAAg                                                                                                          >  2:77961/1‑140 (MQ=255)           tGTTAAAGTTCTTGCGAATCAGGTACATCGTCAGGTAGCCGATAAAGACCACCAGGTAGGATTGCATGAACGGTTTGAACCACATTTTGCGCCGCACTTCGAGCGGAAGGTCCATGGTCGGCTTGCGAACCTGGTTTAAg                                                                                                          >  2:615647/1‑140 (MQ=255)                     cTTGCGAATCAGGTACATCGTCAGGTAGCCGATAAAGACCACCAGGTAGGATTGCATGAACGGTTTGAACCACATTTTGCGCCGCACTTCGAGCGGAAGGTCCATGGTCGGCTTGCGAACCTGGTTTAAGAAAGc                                                                                                     >  1:288855/1‑135 (MQ=255)                     cTTGCGAATCAGGTACATCGTCAGGTAGCCGATAAAGACCACCAGGTAGGATTGCATGAACGGTTTGAACCACATTTTGCGCCGCACTTCGAGCGGAAGGTCCATGGTCGGCTTGCGAACCTGGTTTAAGAAAGc                                                                                                     <  2:288855/135‑1 (MQ=255)                     cTTGCGAATCAGGTACATCGTCAGGTAGCCGATAAAGACCACCAGGTAGGATTGCATGAACGGTTTGAACCACATTTTGCGCCGCACTTCGAGCGGAAGGTCCATGGTCGGCTTGCGAACCTGGTTTAAGAAAGCCAGCa                                                                                                >  2:303230/1‑140 (MQ=255)                     cTTGCGAATCAGGTACATCGTCAGGTAGCCGATAAAGACCACCAGGTAGGATTGCATGAACGGTTTGAACCACATTTTGCGCCGCACTTCGAGCGGAAGGTCCATGGTCGGCTTGCGAACCTGGTTTAAGAAAGCCAGCa                                                                                                >  2:213167/1‑140 (MQ=255)                     cTTGCGAATCAGGTACATCGTCAGGTAGCCGATAAAGACCACCAGGTAGGATTGCATGAACGGTTTGAACCACATTTTGCGCCGCACTTCGAGCGGAAGGTCCATGGTCGGCTTGCGAACCTGGTTTAAGAAAGCCAGCa                                                                                                >  2:213022/1‑140 (MQ=255)                          gAATCAGGTACATCGTCAGGTAGCCGATAAAGACCACCAGGTAGGATTGCATGAACGGTTTGAACCACATTTTGCGCCGCACTTCGAGCGGAAGGTCCATGGTCGGCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGt                                                                                           <  2:724333/140‑1 (MQ=255)                            aTCAGGTACATCGTCAGGTAGCCGATAAAGACCACCAGGTAGGATTGCATGAACGGTTTGAACCACATTTTGCGCCGCACTTCGAGCGGAAGGTCCATGGTCGGCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTa                                                                                         <  1:74700/140‑1 (MQ=255)                            aTCAGGTACATCGTCAGGTAGCCGATAAAGACCACCAGGTAGGATTGCATGAACGGTTTGAACCACATTTTGCGCCGCACTTCGAGCGGAAGGTCCATGGTCGGCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTa                                                                                         <  2:513888/140‑1 (MQ=255)                               aGGTACATCGTCAGGTAGCTGATAAAGACCACCAGGTAGGATTGCATGAACGGTTTGAACCACATTTTGCGCCGCACTTCGAGCGGAAGGTCCATGGTCGGCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTc                                                                                      <  1:364576/140‑1 (MQ=255)                                  tACATCGTCAGGTAGCCGATAAAGACCACCAGGTAGGATTGCATGAACGGTTTGAACCACATTTTGCGCCGCACTTCGAGCGGAAGGTCCATGGTCGGCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTg                                                                                   <  2:689584/140‑1 (MQ=255)                                  tACATCGTCAGGTAGCCGATAAAGACCACCAGGTAGGATTGCATGAACGGTTTGAACCACATTTTGCGCCGCACTTCGAGCGGAAGGTCCATGGTCGGCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTg                                                                                   <  1:40053/140‑1 (MQ=255)                                  tACATCGTCAGGTAGCCGATAAAGACCACCAGGTAGGATTGCATGAACGGTTTGAACCACATTTTGCGCCGCACTTCGAGCGGAAGGTCCATGGTCGGCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTg                                                                                   <  1:85325/140‑1 (MQ=255)                                  tACATCGTCAGGTAGCCGATAAAGACCACCAGGTAGGATTGCATGAACGGTTTGAACCACATTTTGCGCCGCACTTCGAGCGGAAGGTCCATGGTCGGCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTg                                                                                   <  2:208562/140‑1 (MQ=255)                                     aTCGTCAGGTAGCCGATAAAGACCACCAGGTAGGATTGCATGAACGGTTTGAACCACATTTTGCGCCGCACTTCGAGCGGAAGGTCCATGGTCGGCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGaaa                                                                                >  2:566262/1‑140 (MQ=255)                                         tCAGGTAGCCGATAAAGACCACCAGGTAGGATTGCATGAACGGTTTGAACCACATTTTGCGCCGCACTTCGAGCGGAAGGTCCATGGTCGGCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGaa                                                                            >  1:556468/1‑140 (MQ=255)                                          cAGGTAGCCGATAAAGACCACCAGGTAGGATTGCATGAACGGTTTGAACCACATTTTGCGCCGCACTTCGAGCGGAAGGTCCATGGTCGGCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAAt                                                                           >  2:371258/1‑140 (MQ=255)                                            ggTAGCCGATAAAGACCACCAGGTAGGATTGCATGAACGGTTTGAACCACATTTTGCGCCGCACTTCGAGCGGAAGGTCCATGGTCGGCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTCACTCCTGAAATGAATAc                                                                         <  1:303230/140‑1 (MQ=255)                                                gacGATAAAGACCACCAGGTAGGATTGCATGAACGGTTTGAACCACATTTTGCGCCGCACTTCGAGCGGAAGGTCCATGGTCGGATTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGc                                                                     <  2:608176/138‑1 (MQ=255)                                                  cGATAAAGACCACCAGGTAGGATTGCATGAACGGTTTGAACCACATTTTGCGCCGCACTTCGAGCGGAAGGTCCATGGTCGGCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGt                                                                   <  1:629938/140‑1 (MQ=255)                                                      aaaGACCACCAGGTAGGATTGCATGAACGGTTTGAACCACATTTTGCGCCGCACTTCGAGCGGAAGGTCCATGGTCGGCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAgg                                                               <  1:77961/140‑1 (MQ=255)                                                       aaGACCACCAGGTAGGATTGCATGAACGGTTTGAACCACATTTTGCGCCGCACTTCGAGCGGAAGGTCCATGGTCGGCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGgc                                                              <  2:685109/140‑1 (MQ=255)                                                           ccaccaGGTAGGATTGCATGAACGGTTTGAACCACATTTTGCGCCGCACTTCGAGCGGAAGGTCCATGGTCGGCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCg                                                                    >  1:11408/1‑130 (MQ=255)                                                           ccaccaGGTAGGATTGCATGAACGGTTTGAACCACATTTTGCGCCGCACTTCGAGCGGAAGGTCCATGGTCGGCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCg                                                                    <  2:11408/130‑1 (MQ=255)                                                            caccaGGTAGGATTGCATGAACGGTTTGAACCACATTTTGCGCCGCACTTCGAGCGGAAGGTCCATGGTCGGCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGgcatgca                                                         <  2:474377/140‑1 (MQ=255)                                                              ccaGGTAGGATTGCATGAACGGTTTGAACCACATTTTGCGCCGCACTTCGAGCGGAAGGTCCATGGTCGGCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCg                                                                    <  2:123235/127‑1 (MQ=255)                                                              ccaGGTAGGATTGCATGAACGGTTTGAACCACATTTTGCGCCGCACTTCGAGCGGAAGGTCCATGGTCGGCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCg                                                                    >  1:123235/1‑127 (MQ=255)                                                               caGGTAGGATTGCATGAACGGTTTGAACCACATTTTGCGCCGCACTTCGAGCGGAAGGTCCATGGTCGGCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTg                                                      >  1:717779/1‑140 (MQ=255)                                                                       aTTGCATGAACGGTTTGAACCACATTTTGCGCCGCACTTCGAGCGGAAGGTCCATGGTCGGCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATc                                              <  1:213022/140‑1 (MQ=255)                                                                           cATGAACGGTTTGAACCACATTTTGCGCCGCACTTCGAGCGGAAGGTCCATGGTCGGCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATcagca                                          >  2:349597/1‑140 (MQ=255)                                                                            aTGAACGGTTTGAACCACATTTTGCGCCGCACTTCGAGCGGAAGGTCCATGGTCGGCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATcagcag                                         <  1:349597/140‑1 (MQ=255)                                                                                  ggTTTGAACCACATTTTGCGCCGCACTTCGAGCGGAAGGTCCATGGTCGGCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATc                                   <  1:213167/140‑1 (MQ=255)                                                                                              aTTTTGCGCCGCACTTCGAGCGGAAGGTCCATGGTCGGCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATa                       >  1:580339/1‑140 (MQ=255)                                                                                                                   ggAAGGTCCATGGTCGGCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGGaa  >  1:427485/1‑140 (MQ=255)                                                                                                                             |                                                                                                                                  TTCTGCGCGATGTTAAAGTTCTTGCGAATCAGGTACATCGTCAGGTAGCCGATAAAGACCACCAGGTAGGATTGCATGAACGGTTTGAACCACATTTTGCGCCGCACTTCGAGCGGAAGGTCCAGGGTCGGCTTGCGAACCTGGTTTAAGAAAGCCAGCATGGGTTACTCCTGAAATGAATACCTGCGTGAGGCATGCATTGTAAAAATCAGCAGCAAATCAGGCCTGAGATAGCGTCCAGGCAAAACCTGGAA  >  NC_000913/3847007‑3847260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑