Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,221,548 C→A V314F (GTT→TTT)  arpA ← regulator of acetyl CoA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,221,5480CA96.4% 186.5 / 0.4 55V314F (GTT→TTT) arpAregulator of acetyl CoA synthetase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (20/33);  total (21/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

AGTTGCGTAAGTTGGTGATTCTGACCAATTGCCATTAGCAAATTCATCGTTGCAGGTGATAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTA  >  NC_000913/4221412‑4221681
                                                                                                                                        |                                                                                                                                     
aGTTGCGTAAGTTGGTGATTCTGACCAATTGCCATTAGCAAATTCATCGTTGCAGGTGATAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAga                                                                                                                                    <  2:674154/140‑1 (MQ=255)
     cGTAAGTTGGTGATTCTGACCAATTGCCATTAGCAAATTCATCGTTGCAGGTGATAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAgattgat                                                                                                                               >  2:412946/1‑140 (MQ=255)
      gTAAGTTGGTGATTCTGACCAATTGCCATTAGCAAATTCATCGTTGCAGGTGATAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAgattgatt                                                                                                                              <  2:696397/140‑1 (MQ=255)
             ggTGATTCTGACCAATTGCCATTAGCAAATTCATCGTTGCAGGTGATAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACa                                                                                                                       >  1:142835/1‑140 (MQ=255)
               tGATTCTGACCAATTGCCATTAGCAAATTCATCGTTGCAGGTGATAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATg                                                                                                                     <  2:142835/140‑1 (MQ=255)
                 aTTCTGACCAATTGCCATTAGCAAATTCATCGTTGCAGGTGATAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGAt                                                                                                                   >  1:502436/1‑140 (MQ=255)
                  ttCTGACCAATTGCCATTAGCAAATTCATCGTTGCAGGTGATAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATc                                                                                                                  >  2:569133/1‑140 (MQ=255)
                     tGACCAATTGCCATTAGCAAATTCATCGTTGCAGGTGATAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACTTGATCAt                                                                                                                <  1:156466/139‑1 (MQ=255)
                     tGACCAATTGCCATTAGCAAATTCATCGTTGCAGGTGATAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACTTGATCAt                                                                                                                >  2:156466/1‑139 (MQ=255)
                       aCCAATTGCCATTAGCAAATTCATCGTTGCAGGTGATAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTg                                                                                                             <  1:412946/140‑1 (MQ=255)
                         cAATTGCCATTAGCAAATTCATCGTTGCAGGTGATAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGAc                                                                                                           <  2:657155/140‑1 (MQ=255)
                               ccATTAGCAAATTCATCGTTGCAGGTGATAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTc                                                                                                     >  1:376701/1‑140 (MQ=255)
                                  ttAGCAAATTCATCGTTGCAGGTGATAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCagg                                                                                                  <  2:228597/140‑1 (MQ=255)
                                   tAGCAAATTCATCGTTGCAGGTGATAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCagga                                                                                                 <  2:475592/140‑1 (MQ=255)
                                       aaaTTCATCGTTGCAGGTGATAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCt                                                                                             <  2:675879/140‑1 (MQ=255)
                                          ttCATCGTTGCAGGTGATAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCtata                                                                                          >  1:430686/1‑140 (MQ=255)
                                            cATCGTTGCAGGTGATAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAAt                                                                                        >  1:284392/1‑140 (MQ=255)
                                               cGTTGCAGGTGATAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTa                                                                                     >  2:166320/1‑140 (MQ=255)
                                                         gATAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATaaa                                                                           >  1:31736/1‑140 (MQ=255)
                                                          aTAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATaaaa                                                                          <  2:376701/140‑1 (MQ=255)
                                                            aaTGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAAt                                                                                        <  1:405998/124‑1 (MQ=255)
                                                            aaTGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAAt                                                                                        >  2:405998/1‑124 (MQ=255)
                                                            aaTGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATc                                                                        >  1:613917/1‑140 (MQ=255)
                                                                 aGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCa                                                                   >  2:218563/1‑140 (MQ=255)
                                                                 aGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCa                                                                   <  1:205029/140‑1 (MQ=255)
                                                                   gggTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATATTCTACATTCATAAAATCCa                                                                      >  2:99705/1‑135 (MQ=255)
                                                                   gggTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCa                                                                      <  1:651325/135‑1 (MQ=255)
                                                                   gggTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCa                                                                      >  2:651325/1‑135 (MQ=255)
                                                                   gggTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCaga                                                                 <  1:218563/140‑1 (MQ=255)
                                                                   gggTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAAAAACATGATCATTTGACCATTTCAGGAGGCTATATTCTACATTCATAAAATCCa                                                                      <  1:99705/135‑1 (MQ=255)
                                                                      ttATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGaa                                                              <  2:488730/140‑1 (MQ=255)
                                                                        aTTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATa                                                            <  1:700067/140‑1 (MQ=255)
                                                                          ttCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAgt                                                          <  2:267427/140‑1 (MQ=255)
                                                                          ttCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAgt                                                          <  1:301963/140‑1 (MQ=255)
                                                                          ttCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAgt                                                          <  2:414014/140‑1 (MQ=255)
                                                                            cATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAgtgt                                                        <  2:635988/140‑1 (MQ=255)
                                                                             aTCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGtt                                                       <  1:378566/140‑1 (MQ=255)
                                                                                     aTCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATa                                                            >  2:407450/1‑127 (MQ=255)
                                                                                     aTCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATa                                                            <  1:407450/127‑1 (MQ=255)
                                                                                        cTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTctct                                            <  2:570321/140‑1 (MQ=255)
                                                                                           ttAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTg                                         >  2:301255/1‑140 (MQ=255)
                                                                                             aaCATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCt                                       >  2:386545/1‑140 (MQ=255)
                                                                                             aaCATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCt                                       <  1:479430/140‑1 (MQ=255)
                                                                                               cATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCttt                                     >  2:54471/1‑140 (MQ=255)
                                                                                                aTCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTc                                    <  2:430686/140‑1 (MQ=255)
                                                                                                         tGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAAt                           >  1:61049/1‑140 (MQ=255)
                                                                                                         tGTTCTTTCGGAAGTGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAAt                           <  2:30634/140‑1 (MQ=255)
                                                                                                             cTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCtt                       >  1:183708/1‑140 (MQ=255)
                                                                                                                       tGCTGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCgt             <  1:54471/140‑1 (MQ=255)
                                                                                                                          tGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCgttag          <  2:61049/140‑1 (MQ=255)
                                                                                                                          tGGAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCgttag          <  2:613917/140‑1 (MQ=255)
                                                                                                                           ggAATTATTGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCgttagt         <  2:284392/140‑1 (MQ=255)
                                                                                                                                tattGCAAAAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACAt    <  1:597464/140‑1 (MQ=255)
                                                                                                                                  ttGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTa  <  2:183708/140‑1 (MQ=255)
                                                                                                                                  ttGCAAAAGATTGATTATTAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTa  <  2:403865/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                     
AGTTGCGTAAGTTGGTGATTCTGACCAATTGCCATTAGCAAATTCATCGTTGCAGGTGATAATGGAGGGGTTATTTCATCCACAGATCCTTTTAACATCAATAGCTGTTCTTTCGGAAGTGCTGGAATTATTGCAACAGATTGATTAATAACATGATCATTTGACCATTTCAGGAGGCTATAATCTACATTCATAAAATCCATCAGAGAATAGTGTTTAATATTCTCTTTGCTTTCGCTCATAATTCTTATTTTATCGTTAGTTACATTA  >  NC_000913/4221412‑4221681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑