Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	385,349	C→A	Q40K (CAG→AAG)	tauA →	taurine ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	385,349	0	C	A	100.0%	155.0 / NA	45	Q40K (CAG→AAG)	tauA	taurine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (20/25);  total (20/25)

ACAGGGGAGCAGACAATCATGGCAATTTCATCGCGTAACACACTTCTTGCCGCACTGGCATTCATCGCTTTTCAGGCACAGGCGGTGAACGTCACCGTGGCGTATC‑AAACCTCAGCCGAACCGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGACTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTGCAAATCGGCAACCTCGGTTCCAGCCCGTTAG  >  NC_000913/385214‑385481                                                                                                                                         |                                                                                                                                     aCAGGGGAGCAGACAATCATGGCAATTTCATCGCGTAACACACTTCTTGCCGCACTGGCATTCATCGCTTTTCAGGCACAGGCGGTGAACGTCACCGTGGCGTATC‑AAACCTCAGCCGAACCGGCGAAAGTGGCTAAGGc                                                                                                                                  <  1:489661/140‑1 (MQ=255)  cAGGGGAGCAGACAATCATGGCAATTTCATCGCGTAACACACTTCTTGCCGCACTGGCATTCATCGCTTTTCAGGCACAGGCGGTGAACGTCACCGTGGCGTATC‑AAACCTCAGCCGAACCGGCGAAAGTGGCTAAGGcc                                                                                                                                 <  1:486869/140‑1 (MQ=255)     gggAGCAGACAATCATGGCAATTTCATCGCGTAACACACTTCTTGCCGCACTGGCATTCATCGCTTTTCAGGCACAGGCGGTGAACGTCACCGTGGCGTATC‑AAACCTCAGCCGAACCGGCGAAAGTGGCTAAGGCCGac                                                                                                                              >  1:684581/1‑140 (MQ=255)          cAGACAATCATGGCAATTTCATCGCGTAACACACTTCTTGCCGCACTGGCATTCATCGCTTTTCAGGCACAGGCGGTGAACGTCACCGTGGCGTATC‑AAACCTCAGCCGAACCGGCGAAAGTGGCTAAgg                                                                                                                                   <  2:398046/130‑1 (MQ=255)          cAGACAATCATGGCAATTTCATCGCGTAACACACTTCTTGCCGCACTGGCATTCATCGCTTTTCAGGCACAGGCGGTGAACGTCACCGTGGCGTATC‑AAACCTCAGCCGAACCGGCGAAAGTGGCTAAgg                                                                                                                                   >  1:398046/1‑130 (MQ=255)          cAGACAATCATGGCAATTTCATCGCGTAACACACTTCTTGCCGCACTGGCATTCATCGCTTTTCAGGCACAGGCGGTGAACGTCACCGTGGCGTATC‑AAACCTCAGCCGAACCGGCGAAAGTGGCTAAGGCCGACAacac                                                                                                                         >  1:50964/1‑140 (MQ=255)          cAGACAATCATGGCAATTTCATCGCGTAACACACTTCTTGCCGCACTGGCATTCATCGCTTTTCAGGCACAGGCGGTGAACGTCACCGTGGCGGATC‑AAACCTCAACCGAAACCGCCAAAGGGGCTAAGGCCGACAacac                                                                                                                         >  1:531190/1‑140 (MQ=255)               aaTCATGGCAATTTCATCGCGTAACACACTTCTTGCCGCACTGGCATTCATCGCTTTTCAGGCACAGGCGGTGAACGGCACCGGGGCGGCTC‑AAACCTCAGCCGAACCGGGGAAAGTGGCTAAGGCCGACAACACCTTTg                                                                                                                    >  1:144498/1‑140 (MQ=255)                 tCATGGCAATTTCATCGCGTAACACACTTCTTGCCGCACTGGCATTCATCGCTTTTCAGGCACAGGCGGTGAACGTCACCGTGGCGTATC‑AAACCTCAGCAGAACCGGCGAAAGTGGCTAAGGcc                                                                                                                                 <  1:676354/125‑1 (MQ=255)                 tCATGGCAATTTCATCGCGTAACACACTTCTTGCCGCACTGGCATTCATCGCTTTTCAGGCACAGGCGGTGAACGTCACCGTGGCGTATC‑AAACCTCAGCAGAACCGGCGAAAGTGGCTAAGGcc                                                                                                                                 >  2:676354/1‑125 (MQ=255)                       cAATTTCATCGCGTAACACACTTCTTGCCGCACTGGCATTCATCGCTTTTCAGGCACAGGCGGTGAACGTCACCGTGGCGTATC‑AAACCTCAGCCGAACCGGCGAAAGTGGCTAAGGCCGACAACACCTTTGCTaaagaa                                                                                                            <  2:559774/140‑1 (MQ=255)                         aTTTCATCGCGTAACACACTTCTTGCCGCACTGGCATTCATCGCTTTTCAGGCACAGGCGGTGAACGTCACCGTGGCGTATC‑AAACCTCAGCCGAACCGGCGAAAGTGGCTAAGGCCGACAACACCTTTGCTaaagaaag                                                                                                          <  2:615533/140‑1 (MQ=255)                              aTCGCGTAACACACTTCTTGCCGCACTGGCATTCATCGCTTTTCAGGCACAGGCGGTGAACGTCACCGTGGCGTATC‑AAACCTCAGCCGAACCGGCGAAAGTGGCTAAGGCCGACAACACCTTTGCTAAAGAAAGCGGAg                                                                                                     <  1:584736/140‑1 (MQ=255)                              aTCGCGTAACACACTTCTTGCCGCACTGGCATTCATCGCTTTTCAGGCACAGGCGGTGAACGTCACCGTGGCGTATC‑AAACCTCAGCCGAACCGGCGAAAGTGGCTAAGGCCGACAACACCTTTGCTAAAGAAAGCGGAg                                                                                                     <  1:635930/140‑1 (MQ=255)                                     aaCACACTTCTTGCCGCACTGGCATTCATCGCTTTTCAGGCACAGGCGGTGAACGTCACCGTGGCGTATC‑AAACCTCAGCCGAACCGGCGAAAGTGGCTAAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGt                                                                                              >  2:83514/1‑140 (MQ=255)                                       cacacTTCTTGCCGCACTGGCATTCATCGCTTTTCAGGCACAGGCGGTGAACGTCACCGTGGCGTATC‑AAACCTCAGCCGAACCGGCGAAAGTGGCTAAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTgg                                                                                            <  1:236462/140‑1 (MQ=255)                                       cacacTTCTTGCCGCACTGGCATTCATCGCTTTTCAGGCACAGGCGGTGAACGTCACCGTGGCGTATC‑AAACCTCAGCCGAACCGGCGAAAGTGGCTAAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTgg                                                                                            <  2:684581/140‑1 (MQ=255)                                         cacTTCTTGCCGCACTGGCATTCATCGCTTTTCAGGCACAGGCGGTGAACGTCACCGTGGCGTATC‑AAACCTCAGCCGAACCGGCGAAAGTGGCTAAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGAc                                                                                          <  1:530080/140‑1 (MQ=255)                                         cacTTCTTGCCGCACTGGCATTCATCGCTTTTCAGGCACAGGCGGTGAACGTCACCGTGGCGTATC‑AAACCTCAGCCGAACCGGCGAAAGTGGCTAAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGAc                                                                                          <  1:548426/140‑1 (MQ=255)                                              cttGCCGCACTGGCATTCATCGCTTTTCAGGCACAGGCGGCAAAAGTCACCGTGGCGTATCTAAACCTAACCCAAACCGGGCAAAG‑GGGTTAAGTCGACAACACCTTTTCTAAAAAAAACGGGGCAAACGTGGACTGGCg                                                                                     >  2:548426/1‑140 (MQ=255)                                               ttGCCGCACTGGCATTCATCGCTTTTCAGGCACAGGCGGTGAACGTCACCGTGGCGTATC‑AAACCTCAGCCGAACCGGCGAAAGTGGCTAAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGACTGGCGt                                                                                    <  2:328053/140‑1 (MQ=255)                                               ttGCCGCACTGGCATTCATCGCTTTTCAGGCACAGGCGGTGAACGTCACCGTGGCGTATC‑AAACCTCAGCCGAACCGGCGAAAGTGGCTAAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGACTGGCGt                                                                                    <  1:70573/140‑1 (MQ=255)                                                 gCCGCACTGGCATTCATCGCTTTTCAGGCACAGGCGGTGAACGTCACCGTGGCGTATC‑AAACCTCAGCCGAACCGGCGAAAGTGGCTAAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGACTGGCGTaa                                                                                  >  1:696800/1‑140 (MQ=255)                                                 gCCGCACTGGCATTCATCGCTTTTCAGGCACAGGCGGTGAACATCACCGTGGCGTATC‑AAACCTCAGCCGAACCGGCGAAAGTGGCTAAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGACTGGCGTaa                                                                                  >  1:83655/1‑140 (MQ=255)                                                    gCACTGGCATTCATCGCTTTTCAGGCACAGGCGGTGAACGTCACCGTGGCGTATC‑AAACCTCAGCCGAACCGGCGAAAGTGGCTAAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGACTGGCGTAAGtt                                                                               >  1:103484/1‑140 (MQ=255)                                                    gCACTGGCATTCATCGCTTTTCAGGCACAGGCGGTGAACGTCACCGTGGCGTATC‑AAACCTCAGCCGAACCGGCGAAAGTGGCTAAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGACTGGCGTAAGtt                                                                               >  1:371801/1‑140 (MQ=255)                                                    gCACTGGCATTCATCGCTTTTCAGGCACAGGCGGTGAACGTCACCGTGGCGTATC‑AAACCTCAGCCGAACCGGCGAAAGTGGCTAAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGACTGGCGTAAGtt                                                                               >  1:316977/1‑140 (MQ=255)                                                      aCTGGCATTCATCGCTTTTCAGGCACAGGCGGTGAACGTCACCGTGGCGTATC‑AAACCTCAGCCGAACCGGCGAAAGTGGCTAAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGa                                                                                           >  2:90606/1‑126 (MQ=255)                                                      aCTGGCATTCATCGCTTTTCAGGCACAGGCGGTGAACGTCACCGTGGCGTATC‑AAACCTCAGCCGAACCGGCGAAAGTGGCTAAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGa                                                                                           <  1:90606/126‑1 (MQ=255)                                                          gCATTCATCGCTTTTCAGGCACAGGCGGTGAACGTCACCGTGGCGTATC‑AAACCTCAGCCGAACCGGCGAAAGTGGCTAAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGACTGGCGTAAGTTTGACAg                                                                         >  2:587768/1‑140 (MQ=255)                                                           cATTCATCGCTTTTCAGGCACAGGCGGTGAACGTCACCGTGGCGTATC‑AAACCTCAGCCGAACCGGCGAAAGTGGCTAAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGACTGGCGTAAGTTTGACAGc                                                                        <  1:137603/140‑1 (MQ=255)                                                           cATTCATCGCTTTTCAGGCACAGGCGGTGAACGTCACCGTGGCGTATC‑AAACCTCAGCCGAACCGGCGAAAGTGGCTAAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGACTGGCGTAAGTTTGACAGc                                                                        <  1:224617/140‑1 (MQ=255)                                                           cATTCATCGCTTTTCAGGCACAGGCGGTGAACGTCACCGTGGCGTATC‑AAACCTCAGCCGAACCGGCGAAAGTGGCTAAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGACTGGCGTAAGTTTGACAGc                                                                        <  1:587768/140‑1 (MQ=255)                                                                  cGCTTTTCAGGCACAGGCGGGGAAAGTCAACGTGGCGTATC‑AAACCTCAACCGGACCGGCGAAAGTGGCTAAGACCGACAACAACTTTGCTAAAGAAAGCGGAGCAACCATGGACTGGCGCAAGTTTGACAGCGGAACCa                                                                 >  2:50861/1‑140 (MQ=255)                                                                                aGGCGGTGAACGTCACCGTGGCGTATC‑AAACCTCAGCCGAACCGGCGAAAGTGGCTAAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGACTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGgcg                                                   <  2:551687/140‑1 (MQ=255)                                                                                aGGCGGTGAACGTCACCGTGGCGTATC‑AAACCTCAGCCGAACCGGCGAAAGTGGCTAAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGACTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGgcg                                                   <  2:316977/140‑1 (MQ=255)                                                                                                 gTGGCGTATC‑AAACCTCAGCCGAACCGGCGAAAGTGGCTAAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGACTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGt                                  <  2:371801/140‑1 (MQ=255)                                                                                                  tGGCGTATC‑AAACCTCAGCCGAACCGGCGAAAGTGGCTAAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGACTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGtt                                 <  2:50964/140‑2 (MQ=255)                                                                                                  tGGCGTATC‑AAACCTCAGCCGAACCGGCGAAAGTGGCTAAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGACTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTg                                 >  1:97333/1‑140 (MQ=255)                                                                                                  tGGCGTATC‑AAACCTCAGCCGAACCGGCGAAAGTGGCTAAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGACTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTg                                 >  1:82966/1‑140 (MQ=255)                                                                                                    gCGTATC‑AAACCTCAGCCGAACCGGCGAAAGTGGCTAAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGACTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTGCa                               <  2:659634/140‑1 (MQ=255)                                                                                                     cGTATC‑AAACCTCAGCCGAACCGGCGAAAGTGGCTAAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGACTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTGCaa                              >  2:255224/1‑140 (MQ=255)                                                                                                       tATC‑AAACCTCAGCCGAACCGGCGAAAGTGGCTAAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGACTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTGCAAAt                            >  2:71491/1‑140 (MQ=255)                                                                                                         tC‑AAACCTCAGCCGAACCGGCGAAAGTGGCTAAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGACTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTGCAAATCg                          >  2:738/1‑140 (MQ=255)                                                                                                            aaaCCTCAGCCGAACCGGCGAAAGTGGCTAAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGACTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTGCAAATCGGc                        <  2:265232/140‑1 (MQ=255)                                                                                                                   aGCCGAACCGGCGAAAGTGGCTAAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGACTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTTGCTTCAGGCGACGTGCAAATCGGCAACCTCg                 >  2:459499/1‑140 (MQ=255)                                                                                                                        aaCCGGCGAAAGTGGCTAAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGACTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTGCAAATCGGCAACCTCGGTTcc            >  1:716907/1‑140 (MQ=255)                                                                                                                                  aGTGGCTAAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGACTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTGCAAATCGGCAACCTCGGTTCCAGCCCGTTAg  <  2:103484/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     ACAGGGGAGCAGACAATCATGGCAATTTCATCGCGTAACACACTTCTTGCCGCACTGGCATTCATCGCTTTTCAGGCACAGGCGGTGAACGTCACCGTGGCGTATC‑AAACCTCAGCCGAACCGGCGAAAGTGGCTCAGGCCGACAACACCTTTGCTAAAGAAAGCGGAGCAACCGTGGACTGGCGTAAGTTTGACAGCGGAGCCAGCATCGTGCGGGCGCTGGCTTCAGGCGACGTGCAAATCGGCAACCTCGGTTCCAGCCCGTTAG  >  NC_000913/385214‑385481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑