Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,235,936	G→T	S224S (TCG→TCT)	preA →	NAD‑dependent dihydropyrimidine dehydrogenase subunit PreA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,235,936	0	G	T	100.0%	154.6 / NA	44	S224S (TCG→TCT)	preA	NAD‑dependent dihydropyrimidine dehydrogenase subunit PreA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (25/19);  total (25/19)

GTGATATGTGCGAAGTGGCGCTGGCGGCGAAGCGCGGCGGCGCAGATGGCATTGCGGCGATTAACACCGTTAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGGTATCGGCGGCATTGAAACCTGGGAGGATGCGGCTG  >  NC_000913/2235803‑2236072                                                                                                                                      |                                                                                                                                         gTGATATGTGCGAAGTGGCGCTGGCGGCGAAGCGCGGCGGCGCAGATGGCATTGCGGCGATTAACACCGTTAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTAtt                                                                                                                                    >  1:596557/1‑140 (MQ=255)    atatGTGCGAAGTGGCGCTGGCGGCGAAGCGCGGCGGCGCAGATGGCATTGCGGCGATTAACACCGTTAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTcc                                                                                                                                 >  2:88399/1‑140 (MQ=255)       tgtgCGAAGTGGCGCTGGCGGCGAAGCGCGGCGGCGCAGATGGCATTGCGGCGATTAACACCGTTAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGa                                                                                                                              >  2:69841/1‑140 (MQ=255)       tgtgCGAAGTGGCGCTGGCGGCGAAGCGCGGCGGCGCAGATGGCATTGCGGCGATTAACACCGTTAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGa                                                                                                                              <  1:98488/140‑1 (MQ=255)         tgCGAAGTGGCGCTGGCGGCGAAGCGCGGCGGCGCAGATGGCATTGCGGCGATTAACACCGTTAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGata                                                                                                                            >  2:652937/1‑140 (MQ=255)          gCGAAGTGGCGCTGGCGGCGAAGCGCGGCGGCGCAGATGGCATTGCGGCGATTAACACCGTTAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGatat                                                                                                                           <  1:88399/140‑1 (MQ=255)           cGAAGTGGCGCTGGCGGCGAAGCGCGGCGGCGCAGATGGCATTGCGGCGATTAACACCGTTAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATAtt                                                                                                                          >  1:14888/1‑140 (MQ=255)                   cgcTGGCGGCGAAGCGCGGCGGCGCAGATGGCATTGCGGCGATTAACACCGTTAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTaaa                                                                                                                  >  1:682161/1‑140 (MQ=255)                   cgcTGGCGGCGAAGCGCGGCGGCGCAGATGGCATTGCGGCGATTAACACCGTTAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTaaa                                                                                                                  >  2:607325/1‑140 (MQ=255)                       ggcggcGAAGCGCGGCGGCGCAGATGGCATTGCGGCGATTAACACCGTTAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTaa                                                                                                                   <  2:604061/135‑1 (MQ=255)                       ggcggcGAAGCGCGGCGGCGCAGATGGCATTGCGGCGATTAACACCGTTAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTaa                                                                                                                   >  1:604061/1‑135 (MQ=255)                           gcgAAGCGCGGCGGCGCAGATGGCATTGCGGCGATTAACACCGTTAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTaaa                                                                                                          <  1:69841/140‑1 (MQ=255)                                gcgcGGCGGCGCAGATGGCATTGCGGCGATTAACACCGTTAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGa                                                                                                     <  2:121610/140‑1 (MQ=255)                                 cgcgGCGGCGCAGATGGCATTGCGGCGATTAACACCGTTAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGAt                                                                                                    <  2:596557/140‑1 (MQ=255)                                   cggcggcgCAGATGGCATTGCGGCGATTAACACCGTTAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCg                                                                                                  >  2:272152/1‑140 (MQ=255)                                     gcggcgCAGATGGCATTGCGGCGATTAACACCGTTAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGcc                                                                                                <  1:373460/140‑1 (MQ=255)                                     gcggcgCAGATGGCATTGCGGCGATTAACACCGTTAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGcc                                                                                                <  1:272152/140‑1 (MQ=255)                                                               aaCACCGTTAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCAc                                                                      <  1:595675/140‑1 (MQ=255)                                                                     gTTAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATcc                                                                >  2:679261/1‑140 (MQ=255)                                                                     gTTAAATCCATCACCAATACCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATcc                                                                >  1:149485/1‑140 (MQ=255)                                                                      ttAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCAc                                                                      <  1:75388/133‑1 (MQ=255)                                                                      ttAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCAc                                                                      <  1:649756/133‑1 (MQ=255)                                                                      ttAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCAc                                                                      >  2:75388/1‑133 (MQ=255)                                                                      ttAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCAc                                                                      >  2:649756/1‑133 (MQ=255)                                                                      ttAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCa                                                               >  2:208089/1‑140 (MQ=255)                                                                                cACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGcgcg                                                     <  1:607325/140‑1 (MQ=255)                                                                                cACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGcgcg                                                     >  2:599960/1‑140 (MQ=255)                                                                                           aTCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCaa                                            <  2:255422/138‑1 (MQ=255)                                                                                           aTCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCaa                                            >  1:255422/1‑138 (MQ=255)                                                                                           aTCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATc                                          >  2:114410/1‑140 (MQ=255)                                                                                             cTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAg                                        >  2:657001/1‑140 (MQ=255)                                                                                                aaTCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCgg                                     >  1:452744/1‑140 (MQ=255)                                                                                                  tCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGGTa                                   >  1:373870/1‑140 (MQ=255)                                                                                                             gTATGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGGTATCGGCGGCAtt                        <  1:599960/140‑1 (MQ=255)                                                                                                                tGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGGTATcggcg                             >  2:9226/1‑132 (MQ=255)                                                                                                                tGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGGTATcggcg                             <  1:9226/132‑1 (MQ=255)                                                                                                                tGCCGATCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGGTATCGGCGGCATTGaa                     >  1:368136/1‑140 (MQ=255)                                                                                                                     aTCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGGTATCGGCGGCATTGAAACCTg                >  1:648410/1‑140 (MQ=255)                                                                                                                      tCGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGGTATCGGCGGCATTGAAACCTgg               <  1:665683/140‑1 (MQ=255)                                                                                                                       cGTTAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGGTATCGGCGGCATTGAAACCTggg              >  2:307146/1‑140 (MQ=255)                                                                                                                          tAACGGAAAATCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGGTATCGGCGGCATTGAAACCTGggagg           <  2:14888/140‑1 (MQ=255)                                                                                                                                aaaaTCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGGTATCGGCGGCATTGAAACCTGGGAGGATGCgg     <  1:657001/140‑1 (MQ=255)                                                                                                                                  aaTCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGGTATCGGCGGCATTGAAACCTGGGAGGATGCGGCt   >  1:48340/1‑140 (MQ=255)                                                                                                                                   aTCTAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGGTATCGGCGGCATTGAAACCTGGGAGGATGCGGCTg  <  2:149485/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         GTGATATGTGCGAAGTGGCGCTGGCGGCGAAGCGCGGCGGCGCAGATGGCATTGCGGCGATTAACACCGTTAAATCCATCACCAATATCGATCTTAATCAGAAAATCGGTATGCCGATCGTTAACGGAAAATCGAGTATTTCCGGATATTCCGGTAAAGCGGTAAAACCGATCGCCCTGCGCTTCATTCAGCAAATGCGCACCCATCCAGAACTGCGCGATTTCCCAATCAGCGGTATCGGCGGCATTGAAACCTGGGAGGATGCGGCTG  >  NC_000913/2235803‑2236072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑