Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 380,385 +GA coding (458/774 nt) yaiO ← outer membrane protein YaiO

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913380,3831.G94.4% 448.6 / 15.1 126coding (460/774 nt)yaiOouter membrane protein YaiO
Reads supporting (aligned to +/- strand):  ref base . (4/3);  new base G (66/53);  total (70/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01
*NC_000913380,3832.A94.4% 427.0 / 13.7 126coding (460/774 nt)yaiOouter membrane protein YaiO
Reads supporting (aligned to +/- strand):  ref base . (4/3);  new base A (66/53);  total (70/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 126 total aligned reads displayed.

TAAGCACCTGTTCCGCGGCTTAGCCATAGTTGCGTATAACCAGTGCCGCGCGGGTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGT‑‑GATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAGTGTAGCGATAACCGGTCGTGAAAAGCGTTTTTGGTAACAGGGCCAGGTTAATATCCTGA  >  NC_000913/380250‑380513
                                                                                                                                      ||                                                                                                                                  
 aaGCACCTGTTCCGCGGCTTAGCCATAGTTGCGTATAACCAGTGCCGCGCGGGTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGT‑‑Gagatca                                                                                                                             >  1:1330964/1‑135 (MQ=255)
  aGCACCTGTTCCGCGGCTTAGCCATAGTTGCGTATAACCAGTGCCGCGCGGGTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGT‑‑Gagatcac                                                                                                                            <  1:672600/140‑7 (MQ=255)
     aCCTGTTCCGCGGCTTAGCCATAGTTGCGTATAACCAGTGCCGCGCGGGTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGT‑‑Gagatcac                                                                                                                            >  1:626974/1‑131 (MQ=255)
      ccTGTTCCGCGGCTTAGCCATAGTTGCGTATAACCAGTGCCGCGCGGGTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCggg                                                                                                                          <  2:790146/140‑1 (MQ=255)
       cTGTTCCGCGGCTTAGCCATAGTTGCGTATAACCAGTGCCGCGCGGGTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGc                                                                                                                         <  2:773518/140‑1 (MQ=255)
       cTGTTCCGCGGCTTAGCCATAGTTGCGTATAACCAGTGCCGCGCGGGTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGc                                                                                                                         <  2:44477/140‑1 (MQ=255)
       cTGTTCCGCGGCTTAGCCATAGTTGCGTATAACCAGTGCCGCGCGGGTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGc                                                                                                                         <  1:1320452/140‑1 (MQ=255)
       cTGTTCCGCGGCTTAGCCATAGTTGCGTATAACCAGTGCCGCGCGGGTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGT‑‑Gagatcac                                                                                                                            >  2:911345/1‑129 (MQ=255)
       cTGTTCCGCGGCTTAGCCATAGTTGCGTATAACCAGTGCCGCGCGGGTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGT‑‑Gagatcac                                                                                                                            <  1:911345/135‑7 (MQ=255)
        tGTTCCGCGGCTTAGCCATAGTTGCGTATAACCAGTGCCGCGCGGGTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCggg                                                                                                                          >  1:1565774/1‑138 (MQ=255)
        tGTTCCGCGGCTTAGCCATAGTTGCGTATAACCAGTGCCGCGCGGGTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGcc                                                                                                                        >  2:697753/1‑140 (MQ=255)
        tGTTCCGCGGCTTAGCCATAGTTGCGTATAACCAGTGCCGCGCGGGTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGcc                                                                                                                        >  2:747066/1‑140 (MQ=255)
        tGTTCCGCGGCTTAGCCATAGTTGCGTATAACCAGTGCCGCGCGGGTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGc                                                                                                                         <  2:206123/139‑1 (MQ=255)
          ttCCGCGGCTTAGCCATAGTTGCGTATAACCAGTGCCGCGCGGGTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAg                                                                                                                      >  1:1694818/1‑140 (MQ=255)
          ttCCGCGGCTTAGCCATAGTTGCGTATAACCAGTGCCGCGCGGGTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAg                                                                                                                      >  1:847990/1‑140 (MQ=255)
            ccGCGGCTTAGCCATAGTTGCGTATAACCAGTGCCGCGCGGGTCATTCAGACGCACGGAAATCATATTGCTATAACTACCTCCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGta                                                                                                                    <  2:48454/140‑1 (MQ=255)
               cgGCTTAGCCATAGTTGCGTATAACCAGTGCCGCGCGGGTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGc                                                                                                                         <  2:1204357/132‑1 (MQ=255)
               cgGCTTAGCCATAGTTGCGTATAACCAGTGCCGCGCGGGTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGc                                                                                                                         >  1:1204357/1‑132 (MQ=255)
               cgGCTTAGCCATAGTTGCGTATAACCAGTGCCGCGCGGGTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATag                                                                                                                 >  1:30045/1‑140 (MQ=255)
                 gCTTAGCCATAGTTGCGTATAACCAGTGCCGCGCGGGTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGta                                                                                                                    <  1:1502412/135‑1 (MQ=255)
                    tAGCCATAGTTGCGTATAACCAGTGCCGCGCGGGTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGa                                                                                                            >  1:1754629/1‑140 (MQ=255)
                       ccATAGTTGCGTATAACCAGTGCCGCGCGGGTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATAc                                                                                                         <  2:197434/140‑1 (MQ=255)
                          tAGTTGCGTATAACCAGTGCCGCGCGGGTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATAcgcc                                                                                                      >  1:1606464/1‑140 (MQ=255)
                           aGTTGCGTATAACCAGTGCCGCGCGGGTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATAcgccg                                                                                                     <  1:1014201/140‑1 (MQ=255)
                                cGTATAACCAGTGCCGCGCGGGTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTg                                                                                                >  2:1550180/1‑140 (MQ=255)
                                cGTATAACCAGTGCCGCGCGGGTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTg                                                                                                >  1:818875/1‑140 (MQ=255)
                                 gTATAACCAGTGCCGCGCGGGTCATTCGGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATAcgccg                                                                                                     >  2:628487/1‑134 (MQ=255)
                                 gTATAACCAGTGCCGCGCGGGTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATAcgccg                                                                                                     >  2:289186/1‑134 (MQ=255)
                                 gTATAACCAGTGCCGCGCGGGTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATAcgccg                                                                                                     <  1:289186/134‑1 (MQ=255)
                                 gTATAACCAGTGCCGCGCGGGTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGc                                                                                               <  1:1212526/140‑1 (MQ=255)
                                  tataACCAGTGCCGCGCGGGTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCAAAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATAc                                                                                                         <  2:806518/129‑1 (MQ=255)
                                  tataACCAGTGCCGCGCGGGTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCAAAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATAc                                                                                                         >  1:806518/1‑129 (MQ=255)
                                          gTGCCGCGCGGGTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCg                                                                                      >  1:884493/1‑140 (MQ=255)
                                          gTGCCGCGCGGGTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCCGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCg                                                                                      >  1:507103/1‑140 (MQ=255)
                                                cgcgGGTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCg                                                                                >  2:903491/1‑140 (MQ=255)
                                                cgcgGGTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCg                                                                                >  2:1513035/1‑140 (MQ=255)
                                                cgcgGGTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCg                                                                                >  1:1067382/1‑140 (MQ=255)
                                                 gcgGGTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGa                                                                               >  1:1361473/1‑140 (MQ=255)
                                                 gcgGGTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGa                                                                               >  1:680062/1‑140 (MQ=255)
                                                    ggTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGAcat                                                                            <  2:1361473/140‑1 (MQ=255)
                                                    ggTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGAcat                                                                            <  2:680062/140‑1 (MQ=255)
                                                    ggTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGAcat                                                                            <  2:818875/140‑1 (MQ=255)
                                                     gTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGAcatc                                                                           >  1:804633/1‑140 (MQ=255)
                                                      tCATTCAGACGCACGGAAATCATATTGCTATAACTACCCCCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGTCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGAcatca                                                                          <  2:847990/140‑1 (MQ=255)
                                                      tCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGAcatca                                                                          <  1:757090/140‑1 (MQ=255)
                                                      tCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGAcatca                                                                          <  2:884493/140‑1 (MQ=255)
                                                      tCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGAcatca                                                                          <  2:1754629/140‑1 (MQ=255)
                                                          tCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGt                                                                      <  2:1606464/140‑1 (MQ=255)
                                                           cAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTa                                                                     <  1:1550180/140‑1 (MQ=255)
                                                               cGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAatat                                                                 <  1:697753/140‑1 (MQ=255)
                                                                  aCGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATAtt                                                                <  1:181408/138‑1 (MQ=255)
                                                                  aCGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTa                                                              >  1:923628/1‑140 (MQ=255)
                                                                   cGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAg                                                             >  1:840178/1‑140 (MQ=255)
                                                                   cGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAg                                                             >  2:1358308/1‑140 (MQ=255)
                                                                    ggAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAgt                                                            <  2:840178/140‑1 (MQ=255)
                                                                     gAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAgtg                                                           <  2:160135/140‑1 (MQ=255)
                                                                        aTCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAgt                                                            >  2:1309859/1‑136 (MQ=255)
                                                                            tatTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAgtg                                                           >  2:1509155/1‑133 (MQ=255)
                                                                            tatTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAgtg                                                           <  1:1509155/133‑1 (MQ=255)
                                                                               tGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAGTGTAGCGATAAc                                                 <  1:1513035/140‑1 (MQ=255)
                                                                                gCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAgt                                                            <  2:836975/128‑1 (MQ=255)
                                                                                gCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAgt                                                            >  1:836975/1‑128 (MQ=255)
                                                                                         accaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAGTGTAGCGATAACCGGt                                             <  1:608350/134‑1 (MQ=255)
                                                                                         accaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAGTGTAGCGATAACCGGt                                             >  2:608350/1‑134 (MQ=255)
                                                                                          ccaccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAGTGTAGCGATAACCGGTCGTGaaa                                      >  1:1009908/1‑140 (MQ=255)
                                                                                           caccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAGTGTAGCGATAACCGGt                                             >  2:1309448/1‑132 (MQ=255)
                                                                                           caccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAGTGTAGCGATAACCGGt                                             <  1:1309448/132‑1 (MQ=255)
                                                                                           caccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAGTGTAGCGATAACCGGTCGTGaaaa                                     >  1:1584186/1‑140 (MQ=255)
                                                                                           caccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAGTGTAGCGATAACCGGTCGTGaaaa                                     >  2:1284416/1‑140 (MQ=255)
                                                                                           caccTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAGTGTAGCGATAACCGGTCGTGaaaa                                     >  2:184467/1‑140 (MQ=255)
                                                                                            accTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAGTGTAGCGATAACCGGTCGTGAAAAg                                    <  2:1584186/140‑1 (MQ=255)
                                                                                              cTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAGTGTAGCGATAACCGGTCGTGAAAAGCg                                  >  1:239356/1‑140 (MQ=255)
                                                                                                 cATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAGTGTAGCGATAACCGGTCGTGAAAAGCGttt                               >  1:477337/1‑140 (MQ=255)
                                                                                                 cATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAGTGTAGCGATAACCGGTCGTGAAAAGCGttt                               >  1:1049055/1‑140 (MQ=255)
                                                                                                  aTCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAGTGTAGCGATAACCGGTCGTGAAAAGCGtttt                              >  2:1589247/1‑140 (MQ=255)
                                                                                                     gCTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAGTGTAGCGATAACCGGTCGTGAAAAGCGTTTTTgg                           >  1:401770/1‑140 (MQ=255)
                                                                                                      cTGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAGTGTAGCGATAACCGGTCGTGAAAAGCGTTTTTGGt                          >  2:1095714/1‑140 (MQ=255)
                                                                                                       tGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAGTGTAGCGATAACCGGTCGTGAAAAGCGTTTTTGGTa                         <  1:184467/140‑1 (MQ=255)
                                                                                                       tGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAGTGTAGCGATAACCGGTCGTGAAAAGCGTTTTTGGTa                         <  1:1284416/140‑1 (MQ=255)
                                                                                                       tGGAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAGTGTAGCGATAACCGGTCGTGAAAAGCGTTTTTGGTa                         >  2:1719297/1‑140 (MQ=255)
                                                                                                        ggAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAGTGTAGCGATAACCGGTCGTGAAAAGCGTTTTTGGTaa                        >  2:1488176/1‑140 (MQ=255)
                                                                                                         gAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAGTGTAGCGATAACCGGTCGTGAAAAGCGTTTTTGGTAAc                       <  2:507103/140‑1 (MQ=255)
                                                                                                         gAGTCATAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAGTGTAGCGATAACCGGTCGTGAAAAGCGTTTTTGGTAAc                       <  2:1067382/140‑1 (MQ=255)
                                                                                                              aTAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAGTGTAGCGATAACCGGTCGTGAAAAGCGTTTTTGGTAACAggg                   <  2:1543032/139‑1 (MQ=255)
                                                                                                              aTAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAGTGTAGCGATAACCGGTCGTGAAAAGCGTTTTTGGTAACAGGGc                  <  2:1068309/140‑1 (MQ=255)
                                                                                                              aTAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAGTGTAGCGATAACCGGTCGTGAAAAGCGTTTTTGGTAACAGGGc                  >  2:1403970/1‑140 (MQ=255)
                                                                                                              aTAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAGTGTAGCGATAACCGGTCGTGAAAAGCGTTTTTGGTAACAGGGc                  <  1:1488176/140‑1 (MQ=255)
                                                                                                              aTAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAGTGTAGCGATAACCGGTCGTGAAAAGCGTTTTTGGTAACAGGGc                  >  2:1652468/1‑140 (MQ=255)
                                                                                                               tAATGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAGTGTAGCGATAACCGGTCGTGAAAAGCGTTTTTGGTAACAGGGcc                 <  1:1355234/140‑1 (MQ=255)
                                                                                                                aaTGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAGTGTAGCGATAACCGGTCGTGAAAAGCGTTTTTGGTAACAGGGc                  <  1:984311/138‑1 (MQ=255)
                                                                                                                aaTGGGTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAGTGTAGCGATAACCGGTCGTGAAAAGCGTTTTTGGTAACAGGGCCa                <  1:158672/140‑1 (MQ=255)
                                                                                                                     gTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAGTGTAGCGATAACCGGTCGTGAAAAGCGTTTTTGGTAACAGGGCCa                <  1:638027/135‑1 (MQ=255)
                                                                                                                     gTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAGTGTAGCGATAACCGGTCGTGAAAAGCGTTTTTGGTAACAGGGCCa                >  2:638027/1‑135 (MQ=255)
                                                                                                                     gTATAGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAGTGTAGCGATAACCGGTCGTGAAAAGCGTTTTTGGTAACAGGGCCAGGTTa           >  1:1605772/1‑140 (MQ=255)
                                                                                                                        taGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAGTGTAGCGATAACCGGTCGTGAAAAGCGTTTTTGGTAACAGGGCCAGGTTAata        >  2:1752479/1‑140 (MQ=255)
                                                                                                                         aGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAGTGTAGCGATAACCGGTCGTGAAAAGCGTTTTTGGTAACAGGGCCAGGTTAatat       >  2:31202/1‑140 (MQ=255)
                                                                                                                         aGCGGTAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAGTGTAGCGATAACCGGTCGTGAAAAGCGTTTTTGGTAACAGGGCCAGGTTAatat       >  2:333598/1‑140 (MQ=255)
                                                                                                                              tAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAGTGTAGCGATAACCGGTCGTGAAAAGCGTTTTTGGTAACAGGGCCAGGTTAATATCCTGa  >  2:135241/1‑140 (MQ=255)
                                                                                                                              tAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAGTGTAGCGATAACCGGTCGTGAAAAGCGTTTTTGGTAACAGGGCCAGGTTAATATCCTGa  >  1:1713792/1‑140 (MQ=255)
                                                                                                                              tAGCTGGTGAGATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAGTGTAGCGATAACCGGTCGTGAAAAGCGTTTTTGGTAACAGGGCCAGGTTAATATCCTGa  >  2:531116/1‑140 (MQ=255)
                                                                                                                                      ||                                                                                                                                  
TAAGCACCTGTTCCGCGGCTTAGCCATAGTTGCGTATAACCAGTGCCGCGCGGGTCATTCAGACGCACGGAAATCATATTGCTATAACTACCACCTGCATCGCTGGAGTCATAATGGGTATAGCGGTAGCTGGT‑‑GATCACCGGGCCAGTATAGAGTGATACGCCGCCTTGCCAGGCATCGACTTCGACATCATCGTAATATTTAGTGTAGCGATAACCGGTCGTGAAAAGCGTTTTTGGTAACAGGGCCAGGTTAATATCCTGA  >  NC_000913/380250‑380513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑