Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,926,3660AC24.2% 216.4 / 31.0 95G30G (GGT→GGG) mioCflavoprotein MioC
Reads supporting (aligned to +/- strand):  ref base A (56/16);  new base C (0/23);  total (56/39)
Fisher's exact test for biased strand distribution p-value = 5.78e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 106 total aligned reads displayed.

TCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTG  >  NC_000913/3926227‑3926505
                                                                                                                                            |                                                                                                                                            
tCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTaaaa                                                                                                                                              >  1:1388943/1‑140 (MQ=255)
    ttCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCg                                                                                                                                         <  2:1561848/140‑1 (MQ=255)
      ccTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGCCCGTGCAGCGTTTC‑GGTGGTAAAC‑CCCGcc                                                                                                                                       <  1:1359995/140‑1 (MQ=255)
       cTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCct                                                                                                                                      >  2:1357000/1‑140 (MQ=255)
          cAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCtt                                                                                                                                   >  2:1155064/1‑140 (MQ=255)
                  cATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCttt                                                                                                                           >  2:947162/1‑140 (MQ=255)
                  cATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCttt                                                                                                                           >  1:1329456/1‑140 (MQ=255)
                    tAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTc                                                                                                                         >  2:265134/1‑140 (MQ=255)
                       aaaggagaaaggTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGc                                                                                                                      <  1:1592325/140‑1 (MQ=255)
                        aaggagaaaggTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGcc                                                                                                                     <  1:1749378/140‑1 (MQ=255)
                         aggagaaaggTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCa                                                                                                                    >  2:757808/1‑140 (MQ=255)
                         aggagaaaggTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCa                                                                                                                    >  2:571793/1‑140 (MQ=255)
                         aggagaaaggTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCa                                                                                                                    >  1:1440078/1‑140 (MQ=255)
                          ggagaaaggTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAC‑CCCGCCTCTTCCAGCTTTTCAGCCAg                                                                                                                   <  2:1129646/140‑1 (MQ=255)
                          ggagaaaggTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAg                                                                                                                   >  1:1745955/1‑140 (MQ=255)
                          ggagaaaggTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAg                                                                                                                   <  1:757808/140‑1 (MQ=255)
                            agaaaggTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGgt                                                                                                                 >  1:100899/1‑140 (MQ=255)
                             gaaaggTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGgtg                                                                                                                <  1:981069/140‑1 (MQ=255)
                              aaaggTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGgtgt                                                                                                               >  1:1567174/1‑140 (MQ=255)
                              aaaggTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGgtgt                                                                                                               >  2:48604/1‑140 (MQ=255)
                              aaaggTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGATAAATCTTCTAACAGCGGACCGTGCAACGTTTC‑GGTGGCAAAA‑CCCGGCTCTTCCAGCTTTTCCGCCAGgtgt                                                                                                               >  1:388812/1‑140 (MQ=255)
                               aaggTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGtt                                                                                                              <  1:947162/140‑1 (MQ=255)
                               aaggTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGtt                                                                                                              <  1:1357000/140‑1 (MQ=255)
                                 ggTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCt                                                                                                            >  1:522180/1‑140 (MQ=255)
                                 ggTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCt                                                                                                            >  2:639429/1‑140 (MQ=255)
                                    tGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATACCCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCt                                                                                                         <  2:1567174/140‑1 (MQ=255)
                                    tGTCCGGAATATCCCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGGGGACCGTGCAGCGTTTC‑GGGGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCt                                                                                                         <  1:678661/140‑1 (MQ=255)
                                     gTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAATCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTa                                                                                                        >  1:4217/1‑140 (MQ=255)
                                     gTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGtt                                                                                                              <  2:216524/134‑1 (MQ=255)
                                     gTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGtt                                                                                                              >  1:216524/1‑134 (MQ=255)
                                     gTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTa                                                                                                        >  2:1313072/1‑140 (MQ=255)
                                      tCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGGTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTAc                                                                                                       >  1:1165692/1‑140 (MQ=255)
                                      tCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCT‑ACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACa                                                                                                      >  2:214215/1‑140 (MQ=255)
                                                ctcCGGCCCGGTGGGGGGGGCTAATAACCAGCCAGACCCCGGGGGAGGGAAAATCTTCAAACGGCGGCCCGTGCGGCTTTTG‑GGTGGAAAAC‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCa                                                                                             <  2:1329456/140‑1 (MQ=255)
                                                ctcCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCa                                                                                             >  2:684944/1‑140 (MQ=255)
                                                     gCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCAccgcc                                                                                        >  1:1735287/1‑140 (MQ=255)
                                                          gtgggtggAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAggg                                                                                   >  1:1588929/1‑140 (MQ=255)
                                                           tgggtggAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGt                                                                                  >  1:330569/1‑140 (MQ=255)
                                                           tgggtggAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGt                                                                                  >  2:1471823/1‑140 (MQ=255)
                                                           tgggtggAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGt                                                                                  >  1:163594/1‑140 (MQ=255)
                                                             agcgaggCTGATACCCACCAAGCCCCCGGAGGCAGTTAATTCTTCAAACGGGGGCCGGGGCGGTGTTGG‑GGTGGAAAAC‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGtgc                                                                                <  2:870205/134‑1 (MQ=255)
                                                               gggAGCTGATACCCAGCCAGATCCCGGAGGCAGGAAATTCTTCAAACAGCGGCCCGTGCAGCTTTTC‑GGTGGAAAAC‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGtgctg                                                                              <  2:330569/139‑1 (MQ=255)
                                                                ggAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGtgctgc                                                                             >  1:857062/1‑140 (MQ=255)
                                                                 gAGCTGATACCCAGCCAGACCCCTGGGGCAGTTAAATTTTCAAACGGCGGCCCGTGCGGCGTTTG‑GGTGGAAAAC‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGcc                                                                            <  2:969214/140‑1 (MQ=255)
                                                                  aGCTGATACCCAGCCAGACCCCTGAGGCAGGAAAATCTTCTAACGGCGGCCCGTGCAGCTTTTC‑GGTGGAAAAC‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCg                                                                           <  2:4217/140‑1 (MQ=255)
                                                                   gCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTGAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGc                                                                          >  2:1220442/1‑140 (MQ=255)
                                                                   gCTGAAACCCACCCAGACCCCTGGGGAAGGAAAATTTTCAAACAGGGGCCGGTGCGGCTTTTG‑GGGGGAAAAC‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGc                                                                          <  2:522180/140‑1 (MQ=255)
                                                                       aaaaCCAGCCAGATCCCTGAGGCGGGTAAACCTTCTAACAGCGGCCCGTGCGGCGTTTC‑GGTGGTAAAC‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGAt                                                                      <  2:10926/138‑1 (MQ=255)
                                                                       aTACCCAGCCAGACCCCTGGGGAAGGAAAATCTTCAAACAGCGGACCGTGCGGCTTTTC‑GGTGGAAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGAt                                                                      <  2:1388943/140‑1 (MQ=255)
                                                                       aTAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGAt                                                                      >  1:1615025/1‑140 (MQ=255)
                                                                         aaCCAGCCAGACCCCTGGGGCAGGTAATTTTTCTAAAAGCGGCCGGTGCAGTGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATaa                                                                    <  2:1615025/140‑1 (MQ=255)
                                                                              gccagATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATactgtct                                                               >  1:870205/1‑134 (MQ=255)
                                                                              gccagATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTg                                                               >  1:274477/1‑140 (MQ=255)
                                                                                cagACCCCTGAGGCGGGAAAATCTTCAAACGGCGGACCGTGCAGCTTTTG‑GGTGGAAAAC‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGat                                                             <  2:973253/140‑1 (MQ=255)
                                                                                 agATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGata                                                            >  1:1019862/1‑140 (MQ=255)
                                                                                  gaccccTGAGGCGGGTAATTCTCATAACAGGGGCCGGTGCGGC‑TTTC‑GGGGGGAAAACCCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGatat                                                           <  2:274477/137‑1 (MQ=255)
                                                                                  gATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTgctgt                                                           >  1:1248937/1‑136 (MQ=255)
                                                                                   aTCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATc                                                          >  1:1272997/1‑140 (MQ=255)
                                                                                   aTCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATc                                                          >  1:684717/1‑140 (MQ=255)
                                                                                    ccccgggggCGGGAAATTCTTCAAACGGGGGCCGGGGCAG‑GTTTT‑GGGGGTAAAACCCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCt                                                         <  2:1272997/133‑1 (MQ=255)
                                                                                         gAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCAt                                                    >  2:399702/1‑140 (MQ=255)
                                                                                           ggCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATaa                                                  >  2:1046414/1‑140 (MQ=255)
                                                                                             cAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAAc                                                >  1:1413310/1‑140 (MQ=255)
                                                                                             cAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAAc                                                >  2:1568037/1‑140 (MQ=255)
                                                                                             cAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAAc                                                >  1:1155087/1‑140 (MQ=255)
                                                                                               ggTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCg                                              >  1:27985/1‑140 (MQ=255)
                                                                                               ggTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCg                                              >  1:1079016/1‑140 (MQ=255)
                                                                                               ggTAAATCTTCTAACAGCGAACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGGGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCg                                              >  1:1646683/1‑140 (MQ=255)
                                                                                                gTAATTCTTCAAACGGCGGCCGGTGCAGCTTTTG‑GGGGGAAAAC‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGc                                             <  2:684717/140‑1 (MQ=255)
                                                                                                gTAAATCTTCTAACAGCGGCCCGTGCAGCGTTTC‑GGTGGTAAAC‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGc                                             <  1:639429/140‑1 (MQ=255)
                                                                                                gTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGc                                             >  1:369185/1‑140 (MQ=255)
                                                                                                gTAAATCTTCAAACGGCGGCCCGGGCGGTTTTTG‑GGGGGAAAAC‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGc                                             <  2:163594/140‑1 (MQ=255)
                                                                                                aaaattctcctAACGGCGGCGCGGGCAGCTTTTC‑GGTGAAAAAC‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTCCATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGc                                             <  2:1165692/135‑1 (MQ=255)
                                                                                                aaaaaTCTTCAAACAGCGGCCGGGGCAGTTTTTG‑GGTGAAAAAC‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGc                                             <  2:1019862/138‑1 (MQ=255)
                                                                                                  aattcttcaAACGGGGGCCCGTGCGGTGTTTC‑GGTGAAAAAT‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCa                                           <  1:48604/137‑1 (MQ=255)
                                                                                                    aTCTTCTAACAGCGGCCCGTGCAGCTTTTC‑GGTGGTAAAC‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCAcc                                         <  1:265134/140‑1 (MQ=255)
                                                                                                    aTCTTCTAACAGCGGACCGTGCAGCGTTTG‑GGTGGTAAAC‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCAcc                                         <  1:194872/140‑1 (MQ=255)
                                                                                                     ttttcaAACGGCGGCCCGGGCGGGTTTTG‑GGTGGAAAAC‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCt                                        <  2:100899/138‑1 (MQ=255)
                                                                                                         ctAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTttat                                    >  1:1549099/1‑140 (MQ=255)
                                                                                                          aaacgggggACGGGGCAGC‑TTTT‑GGTGGTAAAACCCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTttatt                                   <  2:1488349/133‑1 (MQ=255)
                                                                                                                cGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGt                             >  2:1485073/1‑140 (MQ=255)
                                                                                                                cGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGt                             >  1:1488349/1‑140 (MQ=255)
                                                                                                                 ggcccGTGCAGCTTTTG‑GGTGGAAAAC‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTg                            <  1:998541/137‑1 (MQ=255)
                                                                                                                 ggACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTg                            >  1:657872/1‑140 (MQ=255)
                                                                                                                   cccGTGCAGCGTTTG‑GGGGGTAAAC‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGc                          <  1:571793/139‑1 (MQ=255)
                                                                                                                   cccGTGC‑GCCTTTCGGGTGGAAAAC‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTTTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGc                          <  2:1549099/139‑1 (MQ=255)
                                                                                                                   aCCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCt                                        >  2:1530182/1‑126 (MQ=255)
                                                                                                                     cGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGt                        >  1:995360/1‑140 (MQ=255)
                                                                                                                       tGCAGCGTTTC‑GGTGGTAAAC‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTg                            <  1:934294/134‑1 (MQ=255)
                                                                                                                       tGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTg                            >  2:934294/1‑134 (MQ=255)
                                                                                                                         cAGGGTTTC‑GGTGGTAAAC‑CCCGCCTCTTCCAGCTTTTGAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTg                    <  1:1220442/140‑1 (MQ=255)
                                                                                                                            cGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTAc                 >  1:1032995/1‑140 (MQ=255)
                                                                                                                             gTTTC‑GGTGGTAAAC‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACg                <  1:1471823/140‑1 (MQ=255)
                                                                                                                             gTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACg                >  2:973239/1‑140 (MQ=255)
                                                                                                                                     tgaaaata‑CCCGCCTCTTCCAGTTTTTCAGTCGGGGGTTGGGTTACATATTCGGCGCCGCCGAGGTTGCTGCCGCTGATAAGAGTGATATCTGCCATAAGCCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGaa         <  2:388812/133‑1 (MQ=255)
                                                                                                                                     gggaaaac‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGaa         <  2:1032995/136‑1 (MQ=255)
                                                                                                                                       gtAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAAcg       >  2:201242/1‑140 (MQ=255)
                                                                                                                                         aaac‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAAcgcg     <  2:1413310/140‑1 (MQ=255)
                                                                                                                                          aaa‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGt    >  1:947111/1‑140 (MQ=255)
                                                                                                                                            a‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTg  <  1:1568037/140‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
TCTGTTCCTGCAATGCTTCATAGAAAGGAGAAAGGTTGTCCGGAATATCTCCGGCACCGTGGGTGGAGCTGATAACCAGCCAGATCCCTGAGGCAGGTAAATCTTCTAACAGCGGACCGTGCAGCGTTTC‑GGTGGTAAAA‑CCCGCCTCTTCCAGCTTTTCAGCCAGGTGTTCTGCTACATATTCGGCACCGCCGAGGGTGCTGCCGCTGATAAGAGTGATATCTGCCATAAACCGCCACCTTTATTAAGAGTGGCGTATTGTACGCTGTGAACGCGTTG  >  NC_000913/3926227‑3926505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑